Pectobacterium phage Zenivior
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8FJI4|A0A3G8FJI4_9CAUD Peptidase_M28 domain-containing protein OS=Pectobacterium phage Zenivior OX=2489635 PE=4 SV=1
MM1 pKa = 7.63 TYY3 pKa = 10.34 YY4 pKa = 10.64 EE5 pKa = 4.26 SADD8 pKa = 3.72 VIITRR13 pKa = 11.84 ARR15 pKa = 11.84 AVQEE19 pKa = 3.92 LRR21 pKa = 11.84 RR22 pKa = 11.84 HH23 pKa = 6.67 GITDD27 pKa = 3.43 TAEE30 pKa = 4.04 FDD32 pKa = 3.64 SDD34 pKa = 3.38 MGICTEE40 pKa = 4.26 YY41 pKa = 10.78 NAQDD45 pKa = 3.39 VLQWLGYY52 pKa = 10.06
Molecular weight: 6.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.036
IPC2_protein 4.406
IPC_protein 4.215
Toseland 4.037
ProMoST 4.368
Dawson 4.19
Bjellqvist 4.355
Wikipedia 4.126
Rodwell 4.062
Grimsley 3.961
Solomon 4.177
Lehninger 4.139
Nozaki 4.329
DTASelect 4.507
Thurlkill 4.088
EMBOSS 4.126
Sillero 4.342
Patrickios 1.99
IPC_peptide 4.177
IPC2_peptide 4.317
IPC2.peptide.svr19 4.255
Protein with the highest isoelectric point:
>tr|A0A3G8FJI0|A0A3G8FJI0_9CAUD Uncharacterized protein OS=Pectobacterium phage Zenivior OX=2489635 PE=4 SV=1
MM1 pKa = 7.67 SYY3 pKa = 10.98 AKK5 pKa = 10.36 KK6 pKa = 9.81 LQKK9 pKa = 10.7 SFTGLSQLQGGALQKK24 pKa = 10.68 RR25 pKa = 11.84 RR26 pKa = 11.84 DD27 pKa = 3.87 KK28 pKa = 11.25 LNQAGVHH35 pKa = 5.77 HH36 pKa = 6.98 EE37 pKa = 4.13 KK38 pKa = 10.53 QRR40 pKa = 11.84 ATFDD44 pKa = 3.36 TNTRR48 pKa = 11.84 ATEE51 pKa = 3.83 KK52 pKa = 10.52 RR53 pKa = 11.84 RR54 pKa = 11.84 GCSKK58 pKa = 10.42 PPRR61 pKa = 11.84 GLL63 pKa = 3.38
Molecular weight: 7.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 9.926
IPC_protein 10.847
Toseland 11.272
ProMoST 11.213
Dawson 11.301
Bjellqvist 11.067
Wikipedia 11.564
Rodwell 11.564
Grimsley 11.33
Solomon 11.55
Lehninger 11.491
Nozaki 11.242
DTASelect 11.067
Thurlkill 11.242
EMBOSS 11.696
Sillero 11.242
Patrickios 11.316
IPC_peptide 11.55
IPC2_peptide 10.175
IPC2.peptide.svr19 8.684
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
12814
50
1263
237.3
26.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.888 ± 0.525
1.116 ± 0.156
6.142 ± 0.233
5.275 ± 0.324
2.895 ± 0.153
7.484 ± 0.289
2.201 ± 0.195
4.675 ± 0.175
4.62 ± 0.322
8.717 ± 0.243
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.607 ± 0.199
4.362 ± 0.268
3.957 ± 0.192
4.464 ± 0.208
5.673 ± 0.341
6.555 ± 0.306
6.594 ± 0.462
7.437 ± 0.237
1.35 ± 0.141
3.988 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here