Apis mellifera associated microvirus 37

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTF9|A0A3S8UTF9_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 37 OX=2494766 PE=4 SV=1
MM1 pKa = 6.67THH3 pKa = 5.37VSKK6 pKa = 10.8FIEE9 pKa = 4.62APQDD13 pKa = 3.77PEE15 pKa = 5.6DD16 pKa = 4.29LLQPSYY22 pKa = 11.57AVVNPKK28 pKa = 9.23TGALKK33 pKa = 10.17EE34 pKa = 4.37YY35 pKa = 7.76PACMAPIAADD45 pKa = 3.55AQRR48 pKa = 11.84LDD50 pKa = 3.71DD51 pKa = 4.76RR52 pKa = 11.84GAEE55 pKa = 3.93IFTKK59 pKa = 10.37GQLVNPLHH67 pKa = 6.75KK68 pKa = 10.58LPSIKK73 pKa = 10.92SMVDD77 pKa = 2.6AAEE80 pKa = 3.89RR81 pKa = 11.84SGRR84 pKa = 11.84LMQMHH89 pKa = 6.72RR90 pKa = 11.84QAQNLKK96 pKa = 10.16LAEE99 pKa = 5.12DD100 pKa = 5.21DD101 pKa = 5.56DD102 pKa = 5.8PDD104 pKa = 3.79FQGYY108 pKa = 8.93DD109 pKa = 3.65RR110 pKa = 11.84NWLEE114 pKa = 4.09EE115 pKa = 4.0NFVTEE120 pKa = 4.71FEE122 pKa = 4.38FEE124 pKa = 4.2FMRR127 pKa = 11.84EE128 pKa = 3.9RR129 pKa = 11.84LPVSAPSQPAPDD141 pKa = 3.87APKK144 pKa = 9.89EE145 pKa = 4.32VPATTPADD153 pKa = 3.76KK154 pKa = 10.76ADD156 pKa = 4.36PPPASSNADD165 pKa = 3.06

Molecular weight: 18.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTM5|A0A3S8UTM5_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 37 OX=2494766 PE=4 SV=1
MM1 pKa = 7.51ARR3 pKa = 11.84RR4 pKa = 11.84GRR6 pKa = 11.84SKK8 pKa = 10.79SSRR11 pKa = 11.84TKK13 pKa = 8.93SSRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84AYY20 pKa = 10.03HH21 pKa = 6.45PPASLPRR28 pKa = 11.84LHH30 pKa = 6.84RR31 pKa = 11.84RR32 pKa = 11.84YY33 pKa = 10.75DD34 pKa = 3.67FAPTPSFDD42 pKa = 3.16TLYY45 pKa = 10.2PSRR48 pKa = 11.84PLRR51 pKa = 11.84RR52 pKa = 11.84PPVQRR57 pKa = 11.84FVFPARR63 pKa = 11.84VLTSPARR70 pKa = 11.84RR71 pKa = 11.84VASVPNRR78 pKa = 11.84YY79 pKa = 8.87IPKK82 pKa = 10.01LSVPVPRR89 pKa = 11.84ITIRR93 pKa = 11.84DD94 pKa = 3.68LVCAKK99 pKa = 9.97RR100 pKa = 11.84AIRR103 pKa = 11.84RR104 pKa = 11.84EE105 pKa = 3.85VLFASGKK112 pKa = 9.88GGSKK116 pKa = 9.18VARR119 pKa = 11.84PKK121 pKa = 9.29FTSVSKK127 pKa = 10.62IKK129 pKa = 10.78CC130 pKa = 3.37

Molecular weight: 14.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1365

130

480

273.0

30.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.399 ± 1.602

0.733 ± 0.3

5.788 ± 0.83

4.762 ± 0.689

4.176 ± 0.553

7.253 ± 1.283

2.711 ± 0.269

4.469 ± 0.474

5.421 ± 0.569

9.304 ± 0.812

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.491 ± 0.315

3.736 ± 0.785

7.106 ± 1.28

4.615 ± 0.633

7.179 ± 1.827

6.593 ± 0.88

5.275 ± 0.395

5.788 ± 0.469

1.538 ± 0.403

3.663 ± 0.567
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski