Halorubrum sp. AJ67

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halorubrum; unclassified Halorubrum

Average proteome isoelectric point is 5.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3364 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V6DWC7|V6DWC7_9EURY Isoform of V6DXC1 Nucleotidyltransferase OS=Halorubrum sp. AJ67 OX=1173487 GN=C483_00770 PE=4 SV=1
MM1 pKa = 7.35SFDD4 pKa = 4.2TIFVPAFGSDD14 pKa = 2.8ASRR17 pKa = 11.84ILEE20 pKa = 4.16IDD22 pKa = 3.55NEE24 pKa = 4.54TGDD27 pKa = 3.73TVAFHH32 pKa = 6.6SVSDD36 pKa = 3.83SVGGIDD42 pKa = 5.22RR43 pKa = 11.84DD44 pKa = 3.46ADD46 pKa = 3.74GNVYY50 pKa = 10.49AIVGQYY56 pKa = 9.49LKK58 pKa = 10.6KK59 pKa = 10.69YY60 pKa = 9.4SPDD63 pKa = 3.22MNLIWTSGEE72 pKa = 4.34TYY74 pKa = 8.7STTVVEE80 pKa = 4.79VADD83 pKa = 3.74NGNAIFVGGGSRR95 pKa = 11.84IIRR98 pKa = 11.84LDD100 pKa = 3.48PDD102 pKa = 3.99GSIVWNSYY110 pKa = 8.13DD111 pKa = 3.82TNIGCLDD118 pKa = 3.47MSYY121 pKa = 11.31DD122 pKa = 3.7GGDD125 pKa = 3.53YY126 pKa = 10.77LYY128 pKa = 11.16VQAADD133 pKa = 3.7DD134 pKa = 4.21SEE136 pKa = 3.84DD137 pKa = 3.36RR138 pKa = 11.84RR139 pKa = 11.84YY140 pKa = 11.06GFANGNDD147 pKa = 3.66SAEE150 pKa = 4.14EE151 pKa = 3.72LVGYY155 pKa = 10.56NDD157 pKa = 4.18DD158 pKa = 3.56SSGILHH164 pKa = 6.86HH165 pKa = 6.72NGSLYY170 pKa = 9.91STEE173 pKa = 4.17LTTSNLWRR181 pKa = 11.84SDD183 pKa = 3.31TFVEE187 pKa = 5.22GSTINDD193 pKa = 3.42GNFTVSNYY201 pKa = 9.67TNLDD205 pKa = 3.11MDD207 pKa = 4.15RR208 pKa = 11.84GGLYY212 pKa = 9.57TAGSGGKK219 pKa = 8.69IAHH222 pKa = 7.13LDD224 pKa = 3.62FDD226 pKa = 6.1GNTQWEE232 pKa = 4.46VSISGSPYY240 pKa = 9.6FVGIDD245 pKa = 3.48VSPDD249 pKa = 3.61TLWASSDD256 pKa = 3.15AGLYY260 pKa = 10.46RR261 pKa = 11.84IDD263 pKa = 4.16KK264 pKa = 10.77SDD266 pKa = 4.8GSVLWKK272 pKa = 9.39NTDD275 pKa = 3.12TQFNSTAHH283 pKa = 6.27EE284 pKa = 4.31SLGGFPNLDD293 pKa = 3.61SYY295 pKa = 11.9GGANWEE301 pKa = 4.11VTVQISGTATVDD313 pKa = 3.33GASTSDD319 pKa = 3.56VEE321 pKa = 4.47ILVIDD326 pKa = 4.09EE327 pKa = 4.92DD328 pKa = 4.68SNAFGGRR335 pKa = 11.84TTTDD339 pKa = 2.85SNGNWTVEE347 pKa = 4.18APDD350 pKa = 3.63STLHH354 pKa = 6.66VVAQYY359 pKa = 11.3KK360 pKa = 10.57DD361 pKa = 3.0ADD363 pKa = 3.67GNVFNTEE370 pKa = 3.7SYY372 pKa = 10.54PYY374 pKa = 10.61VNSS377 pKa = 3.6

Molecular weight: 40.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V6DTB7|V6DTB7_9EURY Isoform of V6DQ87 Transposase IS605 OrfB family protein OS=Halorubrum sp. AJ67 OX=1173487 GN=C468_01355 PE=4 SV=1
MM1 pKa = 7.37GVPVVGGKK9 pKa = 7.96AHH11 pKa = 6.86RR12 pKa = 11.84GVGXXXXXXXPSRR25 pKa = 11.84RR26 pKa = 11.84ARR28 pKa = 11.84GRR30 pKa = 11.84SAPTAASRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84LSATT44 pKa = 3.11

Molecular weight: 3.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

519

2845

3364

684466

37

1978

203.5

22.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.647 ± 0.07

0.791 ± 0.016

8.322 ± 0.057

8.354 ± 0.065

3.43 ± 0.035

8.139 ± 0.054

2.05 ± 0.03

4.52 ± 0.037

2.176 ± 0.032

8.497 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.878 ± 0.024

2.698 ± 0.034

4.7 ± 0.035

2.642 ± 0.029

7.009 ± 0.071

6.186 ± 0.049

6.413 ± 0.042

8.519 ± 0.052

1.158 ± 0.022

2.805 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski