Pteronotus davyi polyomavirus 1
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0GAK6|L0GAK6_9POLY Minor capsid protein OS=Pteronotus davyi polyomavirus 1 OX=1891773 PE=3 SV=1
MM1 pKa = 7.85 APPQKK6 pKa = 10.21 RR7 pKa = 11.84 SPKK10 pKa = 9.76 LPAPAAVPKK19 pKa = 10.55 LLIKK23 pKa = 10.78 GGIEE27 pKa = 4.01 VLDD30 pKa = 4.61 LKK32 pKa = 10.08 TGPDD36 pKa = 3.99 SITQIEE42 pKa = 4.82 LFLNPRR48 pKa = 11.84 MGQPTTSADD57 pKa = 2.93 WVGFSDD63 pKa = 3.81 NVTVSASWSADD74 pKa = 3.01 SPAANQTPCYY84 pKa = 9.18 SCARR88 pKa = 11.84 VSLPMINEE96 pKa = 4.21 DD97 pKa = 3.41 MTSDD101 pKa = 5.06 KK102 pKa = 10.99 ILMWEE107 pKa = 3.97 AVSCKK112 pKa = 9.79 TEE114 pKa = 3.96 VVGVSSLTNVHH125 pKa = 5.55 SFKK128 pKa = 10.99 KK129 pKa = 10.03 KK130 pKa = 9.17 LHH132 pKa = 6.27 DD133 pKa = 3.8 NEE135 pKa = 5.77 GIGLPVEE142 pKa = 4.11 GLNYY146 pKa = 10.63 HH147 pKa = 6.25 MFAVGGEE154 pKa = 3.94 PLEE157 pKa = 4.1 LQFLVEE163 pKa = 4.13 NHH165 pKa = 5.3 HH166 pKa = 5.56 TVYY169 pKa = 10.74 GAGLKK174 pKa = 9.27 TIQNPGASAQTLNPALKK191 pKa = 9.2 GTLDD195 pKa = 3.48 KK196 pKa = 11.46 DD197 pKa = 3.42 GAYY200 pKa = 9.49 PIEE203 pKa = 4.63 AWCPDD208 pKa = 3.35 PSKK211 pKa = 11.45 NEE213 pKa = 3.52 NTRR216 pKa = 11.84 YY217 pKa = 9.48 FGSYY221 pKa = 9.33 TGGQNTPPVLQFTNTVTTILLDD243 pKa = 3.88 EE244 pKa = 5.0 NGVGPLCKK252 pKa = 10.12 GDD254 pKa = 4.39 GLYY257 pKa = 10.67 LSCADD262 pKa = 2.71 IVGFHH267 pKa = 5.61 TQAGNNKK274 pKa = 8.22 MKK276 pKa = 10.48 FRR278 pKa = 11.84 GLPRR282 pKa = 11.84 YY283 pKa = 9.98 FNVTLRR289 pKa = 11.84 KK290 pKa = 9.25 RR291 pKa = 11.84 VVKK294 pKa = 10.6 NPYY297 pKa = 9.06 PVSSLLNSLFTKK309 pKa = 9.75 LIPKK313 pKa = 9.34 MDD315 pKa = 4.33 GQPMSGDD322 pKa = 3.06 NGQVEE327 pKa = 4.68 EE328 pKa = 4.15 VRR330 pKa = 11.84 VYY332 pKa = 10.83 EE333 pKa = 4.28 GTEE336 pKa = 4.02 EE337 pKa = 4.39 VPGDD341 pKa = 3.93 PDD343 pKa = 3.24 MVRR346 pKa = 11.84 YY347 pKa = 8.68 IDD349 pKa = 5.08 KK350 pKa = 9.77 YY351 pKa = 9.33 GQMEE355 pKa = 4.48 TEE357 pKa = 4.6 VPQQ360 pKa = 3.9
Molecular weight: 39.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.26
IPC2_protein 5.347
IPC_protein 5.308
Toseland 5.385
ProMoST 5.499
Dawson 5.372
Bjellqvist 5.474
Wikipedia 5.283
Rodwell 5.308
Grimsley 5.359
Solomon 5.372
Lehninger 5.347
Nozaki 5.537
DTASelect 5.703
Thurlkill 5.461
EMBOSS 5.423
Sillero 5.614
Patrickios 3.973
IPC_peptide 5.385
IPC2_peptide 5.614
IPC2.peptide.svr19 5.618
Protein with the highest isoelectric point:
>tr|L0GCI1|L0GCI1_9POLY Minor capsid protein OS=Pteronotus davyi polyomavirus 1 OX=1891773 PE=3 SV=1
MM1 pKa = 7.49 ALVPYY6 pKa = 9.8 MPDD9 pKa = 3.09 PDD11 pKa = 3.46 IFFPGVSTFARR22 pKa = 11.84 YY23 pKa = 9.82 ANYY26 pKa = 10.46 LDD28 pKa = 4.28 PFHH31 pKa = 7.1 WAPDD35 pKa = 3.59 LYY37 pKa = 10.54 HH38 pKa = 6.53 QVSRR42 pKa = 11.84 WIWEE46 pKa = 4.19 SLMHH50 pKa = 6.47 EE51 pKa = 4.53 GRR53 pKa = 11.84 RR54 pKa = 11.84 QIGYY58 pKa = 10.03 AGQEE62 pKa = 4.04 LVRR65 pKa = 11.84 YY66 pKa = 7.48 TSTTVQDD73 pKa = 3.22 TLARR77 pKa = 11.84 FFEE80 pKa = 4.17 NARR83 pKa = 11.84 WAVSHH88 pKa = 6.96 FSTNIYY94 pKa = 9.89 TSLHH98 pKa = 6.17 DD99 pKa = 4.42 YY100 pKa = 10.5 YY101 pKa = 11.3 RR102 pKa = 11.84 EE103 pKa = 3.99 LPKK106 pKa = 10.7 LKK108 pKa = 10.23 PEE110 pKa = 4.03 QARR113 pKa = 11.84 QLSRR117 pKa = 11.84 LLKK120 pKa = 10.33 EE121 pKa = 4.57 KK122 pKa = 10.64 IPDD125 pKa = 3.54 KK126 pKa = 11.39 VNLEE130 pKa = 5.01 GIDD133 pKa = 3.5 QHH135 pKa = 5.99 HH136 pKa = 6.84 TSAEE140 pKa = 4.23 FIDD143 pKa = 4.69 KK144 pKa = 10.75 VGAPGGANQRR154 pKa = 11.84 HH155 pKa = 5.3 TPDD158 pKa = 2.67 WMLPLILGLYY168 pKa = 10.42 GDD170 pKa = 5.39 LTPTWKK176 pKa = 10.43 KK177 pKa = 9.4 VVEE180 pKa = 4.3 EE181 pKa = 4.52 EE182 pKa = 4.17 EE183 pKa = 5.07 HH184 pKa = 5.63 IHH186 pKa = 7.13 GPSTKK191 pKa = 9.85 KK192 pKa = 10.3 KK193 pKa = 9.09 PKK195 pKa = 8.72 TSSPSSSTKK204 pKa = 9.24 AAYY207 pKa = 9.21 KK208 pKa = 9.81 RR209 pKa = 11.84 RR210 pKa = 11.84 HH211 pKa = 5.67 RR212 pKa = 11.84 SARR215 pKa = 11.84 PQNRR219 pKa = 11.84 ARR221 pKa = 3.86
Molecular weight: 25.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.841
IPC2_protein 8.99
IPC_protein 8.975
Toseland 9.487
ProMoST 9.399
Dawson 9.794
Bjellqvist 9.502
Wikipedia 9.999
Rodwell 9.999
Grimsley 9.897
Solomon 9.838
Lehninger 9.78
Nozaki 9.443
DTASelect 9.502
Thurlkill 9.604
EMBOSS 9.926
Sillero 9.692
Patrickios 4.914
IPC_peptide 9.823
IPC2_peptide 8.039
IPC2.peptide.svr19 7.886
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1781
175
684
356.2
40.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.019 ± 1.241
2.302 ± 0.861
5.222 ± 0.415
6.794 ± 0.518
3.762 ± 0.317
6.008 ± 0.844
2.864 ± 0.466
3.93 ± 0.434
6.569 ± 0.619
9.994 ± 0.583
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.92 ± 0.456
4.099 ± 0.592
6.008 ± 0.781
4.38 ± 0.335
4.773 ± 0.576
6.176 ± 0.524
5.671 ± 0.446
6.064 ± 0.646
1.853 ± 0.299
3.593 ± 0.38
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here