Rhizobium oryziradicis
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4576 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q8ZQ71|A0A1Q8ZQ71_9RHIZ Inositol-1-monophosphatase OS=Rhizobium oryziradicis OX=1867956 GN=BJF95_05810 PE=3 SV=1
MM1 pKa = 7.75 AEE3 pKa = 3.92 HH4 pKa = 6.36 TGEE7 pKa = 4.29 EE8 pKa = 4.15 EE9 pKa = 4.71 QMNCPICDD17 pKa = 3.61 DD18 pKa = 3.55 QFQIGDD24 pKa = 4.24 LCALDD29 pKa = 4.55 IEE31 pKa = 5.77 LGMCHH36 pKa = 6.74 AACLKK41 pKa = 10.27 GSPVVDD47 pKa = 4.58 LDD49 pKa = 3.52 TGEE52 pKa = 4.68 EE53 pKa = 3.94 IGGDD57 pKa = 3.25 MSTFRR62 pKa = 11.84 YY63 pKa = 9.84 GKK65 pKa = 10.08 DD66 pKa = 3.04 VV67 pKa = 3.09
Molecular weight: 7.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 3.884
IPC_protein 3.808
Toseland 3.617
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.528
Solomon 3.77
Lehninger 3.732
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.923
Patrickios 0.477
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A1Q8ZT19|A0A1Q8ZT19_9RHIZ YceI domain-containing protein OS=Rhizobium oryziradicis OX=1867956 GN=BJF95_18085 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.15 GGRR28 pKa = 11.84 KK29 pKa = 9.31 VLVARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.99
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4576
0
4576
1498857
28
2831
327.5
35.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.433 ± 0.046
0.851 ± 0.009
5.754 ± 0.029
5.416 ± 0.032
3.987 ± 0.024
8.023 ± 0.029
2.119 ± 0.016
5.818 ± 0.03
4.129 ± 0.029
10.066 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.734 ± 0.018
3.117 ± 0.02
4.751 ± 0.03
3.452 ± 0.023
6.037 ± 0.035
6.03 ± 0.03
5.473 ± 0.023
7.233 ± 0.03
1.256 ± 0.014
2.322 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here