Tuber aestivum mitovirus
Average proteome isoelectric point is 8.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F6LFY6|F6LFY6_9VIRU RNA-dependent RNA polymerase OS=Tuber aestivum mitovirus OX=1037523 PE=4 SV=1
MM1 pKa = 7.36 MNPFIRR7 pKa = 11.84 LGSLLMGKK15 pKa = 6.16 TTKK18 pKa = 10.47 EE19 pKa = 4.05 LIIGLYY25 pKa = 10.26 SCVQTLHH32 pKa = 7.06 KK33 pKa = 10.06 IYY35 pKa = 10.37 LSQGSRR41 pKa = 11.84 GLAIRR46 pKa = 11.84 LKK48 pKa = 10.79 ASSTYY53 pKa = 11.03 LMKK56 pKa = 10.5 SWSGDD61 pKa = 3.37 PLLDD65 pKa = 3.93 CKK67 pKa = 11.2 AFGPTMSLTPRR78 pKa = 11.84 GIPIWIPSAWRR89 pKa = 11.84 CQISSRR95 pKa = 11.84 NLAIFRR101 pKa = 11.84 VTMTLCNLYY110 pKa = 10.22 RR111 pKa = 11.84 VLPYY115 pKa = 10.07 QGKK118 pKa = 9.84 LKK120 pKa = 10.8 LSTITDD126 pKa = 3.59 DD127 pKa = 4.69 RR128 pKa = 11.84 KK129 pKa = 11.09 GSTPDD134 pKa = 3.68 LMFSFIKK141 pKa = 10.71 DD142 pKa = 3.1 KK143 pKa = 10.62 FAPNITNRR151 pKa = 11.84 VPYY154 pKa = 9.86 PGIFSWDD161 pKa = 3.66 PKK163 pKa = 10.32 PLLTRR168 pKa = 11.84 GPGAQKK174 pKa = 10.5 EE175 pKa = 4.37 KK176 pKa = 10.08 NTMSGFGMALRR187 pKa = 11.84 AIHH190 pKa = 5.34 EE191 pKa = 4.43 TGMLDD196 pKa = 3.51 HH197 pKa = 6.92 LKK199 pKa = 10.57 AYY201 pKa = 10.49 ALYY204 pKa = 10.01 TGLNPQLEE212 pKa = 4.49 SLKK215 pKa = 10.78 VIWEE219 pKa = 4.43 GTRR222 pKa = 11.84 KK223 pKa = 9.49 HH224 pKa = 6.48 CSIGPIGKK232 pKa = 9.04 LAFKK236 pKa = 10.41 QEE238 pKa = 4.09 PGKK241 pKa = 10.85 VRR243 pKa = 11.84 VFAMVDD249 pKa = 4.5 CITQWFLHH257 pKa = 6.46 PLHH260 pKa = 7.0 KK261 pKa = 10.84 YY262 pKa = 9.69 LFSVLRR268 pKa = 11.84 TTKK271 pKa = 10.5 EE272 pKa = 3.65 DD273 pKa = 3.2 ATFDD277 pKa = 3.55 QEE279 pKa = 4.6 KK280 pKa = 10.45 GINLVRR286 pKa = 11.84 LALSKK291 pKa = 11.14 KK292 pKa = 9.26 LDD294 pKa = 3.41 KK295 pKa = 11.2 SVFSFDD301 pKa = 4.34 LSAATDD307 pKa = 4.13 RR308 pKa = 11.84 LPMDD312 pKa = 3.3 IQMVILNSLTPFWLAEE328 pKa = 3.75 KK329 pKa = 10.26 AVRR332 pKa = 11.84 HH333 pKa = 5.04 GVKK336 pKa = 10.43 GGLGDD341 pKa = 3.64 AWADD345 pKa = 3.57 LLVDD349 pKa = 3.81 RR350 pKa = 11.84 DD351 pKa = 4.02 YY352 pKa = 11.8 YY353 pKa = 10.39 LPRR356 pKa = 11.84 WSGYY360 pKa = 10.65 ARR362 pKa = 11.84 DD363 pKa = 3.79 SKK365 pKa = 11.13 VRR367 pKa = 11.84 YY368 pKa = 9.03 AVGQPMGALSSWAMLALTHH387 pKa = 6.72 HH388 pKa = 6.94 MIVQFAAASVGVTGWFKK405 pKa = 11.06 EE406 pKa = 3.79 YY407 pKa = 9.9 MVLGDD412 pKa = 5.78 DD413 pKa = 3.29 IVIYY417 pKa = 10.56 NSEE420 pKa = 3.96 VAKK423 pKa = 10.58 AYY425 pKa = 7.24 STLMGTLGVGISDD438 pKa = 3.93 TKK440 pKa = 11.32 SLTSKK445 pKa = 10.23 IGVFEE450 pKa = 3.99 FAKK453 pKa = 10.55 RR454 pKa = 11.84 LMDD457 pKa = 5.63 LEE459 pKa = 5.37 GPCQGLPLAEE469 pKa = 4.04 FAAARR474 pKa = 11.84 FNLSILFQSFRR485 pKa = 11.84 SRR487 pKa = 11.84 TLYY490 pKa = 10.25 PKK492 pKa = 9.83 ISTFMRR498 pKa = 11.84 FLGFGYY504 pKa = 10.21 KK505 pKa = 10.08 VLGSLGMRR513 pKa = 11.84 LGDD516 pKa = 3.25 MRR518 pKa = 11.84 KK519 pKa = 9.03 RR520 pKa = 11.84 SGYY523 pKa = 10.03 FEE525 pKa = 4.19 TVAYY529 pKa = 10.09 SPLVTDD535 pKa = 4.13 KK536 pKa = 11.25 SVNKK540 pKa = 9.15 WSEE543 pKa = 3.63 FFKK546 pKa = 10.95 RR547 pKa = 11.84 FSTYY551 pKa = 10.99 DD552 pKa = 3.68 LYY554 pKa = 10.7 PLVEE558 pKa = 4.11 ACVYY562 pKa = 9.98 KK563 pKa = 10.48 AYY565 pKa = 10.71 HH566 pKa = 7.23 LIPALTKK573 pKa = 9.9 FDD575 pKa = 3.9 RR576 pKa = 11.84 WNLWRR581 pKa = 11.84 TLFPHH586 pKa = 7.01 FDD588 pKa = 3.63 DD589 pKa = 5.68 KK590 pKa = 11.43 KK591 pKa = 10.08 ILLIPEE597 pKa = 3.75 VAGRR601 pKa = 11.84 HH602 pKa = 5.26 LEE604 pKa = 4.12 SLFTSMLDD612 pKa = 3.49 NKK614 pKa = 8.67 VAKK617 pKa = 10.24 YY618 pKa = 10.22 KK619 pKa = 10.4 ADD621 pKa = 2.98 HH622 pKa = 5.86 SVYY625 pKa = 10.69 VNRR628 pKa = 11.84 LNRR631 pKa = 11.84 AEE633 pKa = 4.32 VPTNDD638 pKa = 3.2 WEE640 pKa = 5.92 AIDD643 pKa = 3.85 TLLSIMEE650 pKa = 4.42 TSPPTSDD657 pKa = 5.05 LLQDD661 pKa = 3.59 PDD663 pKa = 3.8 MNEE666 pKa = 3.88 LGFGLSVSNYY676 pKa = 10.82 DD677 pKa = 3.89 EE678 pKa = 4.99 DD679 pKa = 4.6 NKK681 pKa = 11.28 SKK683 pKa = 9.13 TCSLSLPYY691 pKa = 10.13 TVFTKK696 pKa = 10.29 IPQLLDD702 pKa = 4.64 SIDD705 pKa = 3.9 DD706 pKa = 3.6 WVEE709 pKa = 3.61 PIIPDD714 pKa = 3.25 KK715 pKa = 11.01 HH716 pKa = 6.99 PEE718 pKa = 3.59 VSADD722 pKa = 3.33 VNRR725 pKa = 11.84 PLIHH729 pKa = 6.83 KK730 pKa = 8.61 PGKK733 pKa = 10.42 VFVKK737 pKa = 10.46 GIATGKK743 pKa = 9.91 NEE745 pKa = 3.99 NNKK748 pKa = 9.43 VEE750 pKa = 4.23 ITKK753 pKa = 9.28 MLQDD757 pKa = 4.09 KK758 pKa = 10.71 KK759 pKa = 10.17 FLAYY763 pKa = 9.5 IIEE766 pKa = 4.4 SGKK769 pKa = 8.25 ITIYY773 pKa = 10.87 SPAFQAMIRR782 pKa = 11.84 ASWAKK787 pKa = 9.89 AQSGSKK793 pKa = 10.14
Molecular weight: 89.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.795
IPC2_protein 8.77
IPC_protein 8.639
Toseland 9.385
ProMoST 9.194
Dawson 9.677
Bjellqvist 9.399
Wikipedia 9.838
Rodwell 9.97
Grimsley 9.75
Solomon 9.692
Lehninger 9.648
Nozaki 9.502
DTASelect 9.355
Thurlkill 9.516
EMBOSS 9.823
Sillero 9.619
Patrickios 4.876
IPC_peptide 9.692
IPC2_peptide 7.966
IPC2.peptide.svr19 7.82
Protein with the highest isoelectric point:
>tr|F6LFY6|F6LFY6_9VIRU RNA-dependent RNA polymerase OS=Tuber aestivum mitovirus OX=1037523 PE=4 SV=1
MM1 pKa = 7.36 MNPFIRR7 pKa = 11.84 LGSLLMGKK15 pKa = 6.16 TTKK18 pKa = 10.47 EE19 pKa = 4.05 LIIGLYY25 pKa = 10.26 SCVQTLHH32 pKa = 7.06 KK33 pKa = 10.06 IYY35 pKa = 10.37 LSQGSRR41 pKa = 11.84 GLAIRR46 pKa = 11.84 LKK48 pKa = 10.79 ASSTYY53 pKa = 11.03 LMKK56 pKa = 10.5 SWSGDD61 pKa = 3.37 PLLDD65 pKa = 3.93 CKK67 pKa = 11.2 AFGPTMSLTPRR78 pKa = 11.84 GIPIWIPSAWRR89 pKa = 11.84 CQISSRR95 pKa = 11.84 NLAIFRR101 pKa = 11.84 VTMTLCNLYY110 pKa = 10.22 RR111 pKa = 11.84 VLPYY115 pKa = 10.07 QGKK118 pKa = 9.84 LKK120 pKa = 10.8 LSTITDD126 pKa = 3.59 DD127 pKa = 4.69 RR128 pKa = 11.84 KK129 pKa = 11.09 GSTPDD134 pKa = 3.68 LMFSFIKK141 pKa = 10.71 DD142 pKa = 3.1 KK143 pKa = 10.62 FAPNITNRR151 pKa = 11.84 VPYY154 pKa = 9.86 PGIFSWDD161 pKa = 3.66 PKK163 pKa = 10.32 PLLTRR168 pKa = 11.84 GPGAQKK174 pKa = 10.5 EE175 pKa = 4.37 KK176 pKa = 10.08 NTMSGFGMALRR187 pKa = 11.84 AIHH190 pKa = 5.34 EE191 pKa = 4.43 TGMLDD196 pKa = 3.51 HH197 pKa = 6.92 LKK199 pKa = 10.57 AYY201 pKa = 10.49 ALYY204 pKa = 10.01 TGLNPQLEE212 pKa = 4.49 SLKK215 pKa = 10.78 VIWEE219 pKa = 4.43 GTRR222 pKa = 11.84 KK223 pKa = 9.49 HH224 pKa = 6.48 CSIGPIGKK232 pKa = 9.04 LAFKK236 pKa = 10.41 QEE238 pKa = 4.09 PGKK241 pKa = 10.85 VRR243 pKa = 11.84 VFAMVDD249 pKa = 4.5 CITQWFLHH257 pKa = 6.46 PLHH260 pKa = 7.0 KK261 pKa = 10.84 YY262 pKa = 9.69 LFSVLRR268 pKa = 11.84 TTKK271 pKa = 10.5 EE272 pKa = 3.65 DD273 pKa = 3.2 ATFDD277 pKa = 3.55 QEE279 pKa = 4.6 KK280 pKa = 10.45 GINLVRR286 pKa = 11.84 LALSKK291 pKa = 11.14 KK292 pKa = 9.26 LDD294 pKa = 3.41 KK295 pKa = 11.2 SVFSFDD301 pKa = 4.34 LSAATDD307 pKa = 4.13 RR308 pKa = 11.84 LPMDD312 pKa = 3.3 IQMVILNSLTPFWLAEE328 pKa = 3.75 KK329 pKa = 10.26 AVRR332 pKa = 11.84 HH333 pKa = 5.04 GVKK336 pKa = 10.43 GGLGDD341 pKa = 3.64 AWADD345 pKa = 3.57 LLVDD349 pKa = 3.81 RR350 pKa = 11.84 DD351 pKa = 4.02 YY352 pKa = 11.8 YY353 pKa = 10.39 LPRR356 pKa = 11.84 WSGYY360 pKa = 10.65 ARR362 pKa = 11.84 DD363 pKa = 3.79 SKK365 pKa = 11.13 VRR367 pKa = 11.84 YY368 pKa = 9.03 AVGQPMGALSSWAMLALTHH387 pKa = 6.72 HH388 pKa = 6.94 MIVQFAAASVGVTGWFKK405 pKa = 11.06 EE406 pKa = 3.79 YY407 pKa = 9.9 MVLGDD412 pKa = 5.78 DD413 pKa = 3.29 IVIYY417 pKa = 10.56 NSEE420 pKa = 3.96 VAKK423 pKa = 10.58 AYY425 pKa = 7.24 STLMGTLGVGISDD438 pKa = 3.93 TKK440 pKa = 11.32 SLTSKK445 pKa = 10.23 IGVFEE450 pKa = 3.99 FAKK453 pKa = 10.55 RR454 pKa = 11.84 LMDD457 pKa = 5.63 LEE459 pKa = 5.37 GPCQGLPLAEE469 pKa = 4.04 FAAARR474 pKa = 11.84 FNLSILFQSFRR485 pKa = 11.84 SRR487 pKa = 11.84 TLYY490 pKa = 10.25 PKK492 pKa = 9.83 ISTFMRR498 pKa = 11.84 FLGFGYY504 pKa = 10.21 KK505 pKa = 10.08 VLGSLGMRR513 pKa = 11.84 LGDD516 pKa = 3.25 MRR518 pKa = 11.84 KK519 pKa = 9.03 RR520 pKa = 11.84 SGYY523 pKa = 10.03 FEE525 pKa = 4.19 TVAYY529 pKa = 10.09 SPLVTDD535 pKa = 4.13 KK536 pKa = 11.25 SVNKK540 pKa = 9.15 WSEE543 pKa = 3.63 FFKK546 pKa = 10.95 RR547 pKa = 11.84 FSTYY551 pKa = 10.99 DD552 pKa = 3.68 LYY554 pKa = 10.7 PLVEE558 pKa = 4.11 ACVYY562 pKa = 9.98 KK563 pKa = 10.48 AYY565 pKa = 10.71 HH566 pKa = 7.23 LIPALTKK573 pKa = 9.9 FDD575 pKa = 3.9 RR576 pKa = 11.84 WNLWRR581 pKa = 11.84 TLFPHH586 pKa = 7.01 FDD588 pKa = 3.63 DD589 pKa = 5.68 KK590 pKa = 11.43 KK591 pKa = 10.08 ILLIPEE597 pKa = 3.75 VAGRR601 pKa = 11.84 HH602 pKa = 5.26 LEE604 pKa = 4.12 SLFTSMLDD612 pKa = 3.49 NKK614 pKa = 8.67 VAKK617 pKa = 10.24 YY618 pKa = 10.22 KK619 pKa = 10.4 ADD621 pKa = 2.98 HH622 pKa = 5.86 SVYY625 pKa = 10.69 VNRR628 pKa = 11.84 LNRR631 pKa = 11.84 AEE633 pKa = 4.32 VPTNDD638 pKa = 3.2 WEE640 pKa = 5.92 AIDD643 pKa = 3.85 TLLSIMEE650 pKa = 4.42 TSPPTSDD657 pKa = 5.05 LLQDD661 pKa = 3.59 PDD663 pKa = 3.8 MNEE666 pKa = 3.88 LGFGLSVSNYY676 pKa = 10.82 DD677 pKa = 3.89 EE678 pKa = 4.99 DD679 pKa = 4.6 NKK681 pKa = 11.28 SKK683 pKa = 9.13 TCSLSLPYY691 pKa = 10.13 TVFTKK696 pKa = 10.29 IPQLLDD702 pKa = 4.64 SIDD705 pKa = 3.9 DD706 pKa = 3.6 WVEE709 pKa = 3.61 PIIPDD714 pKa = 3.25 KK715 pKa = 11.01 HH716 pKa = 6.99 PEE718 pKa = 3.59 VSADD722 pKa = 3.33 VNRR725 pKa = 11.84 PLIHH729 pKa = 6.83 KK730 pKa = 8.61 PGKK733 pKa = 10.42 VFVKK737 pKa = 10.46 GIATGKK743 pKa = 9.91 NEE745 pKa = 3.99 NNKK748 pKa = 9.43 VEE750 pKa = 4.23 ITKK753 pKa = 9.28 MLQDD757 pKa = 4.09 KK758 pKa = 10.71 KK759 pKa = 10.17 FLAYY763 pKa = 9.5 IIEE766 pKa = 4.4 SGKK769 pKa = 8.25 ITIYY773 pKa = 10.87 SPAFQAMIRR782 pKa = 11.84 ASWAKK787 pKa = 9.89 AQSGSKK793 pKa = 10.14
Molecular weight: 89.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.795
IPC2_protein 8.77
IPC_protein 8.639
Toseland 9.385
ProMoST 9.194
Dawson 9.677
Bjellqvist 9.399
Wikipedia 9.838
Rodwell 9.97
Grimsley 9.75
Solomon 9.692
Lehninger 9.648
Nozaki 9.502
DTASelect 9.355
Thurlkill 9.516
EMBOSS 9.823
Sillero 9.619
Patrickios 4.876
IPC_peptide 9.692
IPC2_peptide 7.966
IPC2.peptide.svr19 7.82
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
793
793
793
793.0
89.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.683 ± 0.0
1.135 ± 0.0
5.675 ± 0.0
3.657 ± 0.0
5.044 ± 0.0
6.557 ± 0.0
1.892 ± 0.0
5.801 ± 0.0
7.818 ± 0.0
11.475 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.405 ± 0.0
3.026 ± 0.0
5.296 ± 0.0
2.396 ± 0.0
4.792 ± 0.0
7.945 ± 0.0
5.927 ± 0.0
5.549 ± 0.0
2.144 ± 0.0
3.783 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here