Tuber aestivum mitovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Mitovirus; unclassified Mitovirus

Average proteome isoelectric point is 8.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6LFY6|F6LFY6_9VIRU RNA-dependent RNA polymerase OS=Tuber aestivum mitovirus OX=1037523 PE=4 SV=1
MM1 pKa = 7.36MNPFIRR7 pKa = 11.84LGSLLMGKK15 pKa = 6.16TTKK18 pKa = 10.47EE19 pKa = 4.05LIIGLYY25 pKa = 10.26SCVQTLHH32 pKa = 7.06KK33 pKa = 10.06IYY35 pKa = 10.37LSQGSRR41 pKa = 11.84GLAIRR46 pKa = 11.84LKK48 pKa = 10.79ASSTYY53 pKa = 11.03LMKK56 pKa = 10.5SWSGDD61 pKa = 3.37PLLDD65 pKa = 3.93CKK67 pKa = 11.2AFGPTMSLTPRR78 pKa = 11.84GIPIWIPSAWRR89 pKa = 11.84CQISSRR95 pKa = 11.84NLAIFRR101 pKa = 11.84VTMTLCNLYY110 pKa = 10.22RR111 pKa = 11.84VLPYY115 pKa = 10.07QGKK118 pKa = 9.84LKK120 pKa = 10.8LSTITDD126 pKa = 3.59DD127 pKa = 4.69RR128 pKa = 11.84KK129 pKa = 11.09GSTPDD134 pKa = 3.68LMFSFIKK141 pKa = 10.71DD142 pKa = 3.1KK143 pKa = 10.62FAPNITNRR151 pKa = 11.84VPYY154 pKa = 9.86PGIFSWDD161 pKa = 3.66PKK163 pKa = 10.32PLLTRR168 pKa = 11.84GPGAQKK174 pKa = 10.5EE175 pKa = 4.37KK176 pKa = 10.08NTMSGFGMALRR187 pKa = 11.84AIHH190 pKa = 5.34EE191 pKa = 4.43TGMLDD196 pKa = 3.51HH197 pKa = 6.92LKK199 pKa = 10.57AYY201 pKa = 10.49ALYY204 pKa = 10.01TGLNPQLEE212 pKa = 4.49SLKK215 pKa = 10.78VIWEE219 pKa = 4.43GTRR222 pKa = 11.84KK223 pKa = 9.49HH224 pKa = 6.48CSIGPIGKK232 pKa = 9.04LAFKK236 pKa = 10.41QEE238 pKa = 4.09PGKK241 pKa = 10.85VRR243 pKa = 11.84VFAMVDD249 pKa = 4.5CITQWFLHH257 pKa = 6.46PLHH260 pKa = 7.0KK261 pKa = 10.84YY262 pKa = 9.69LFSVLRR268 pKa = 11.84TTKK271 pKa = 10.5EE272 pKa = 3.65DD273 pKa = 3.2ATFDD277 pKa = 3.55QEE279 pKa = 4.6KK280 pKa = 10.45GINLVRR286 pKa = 11.84LALSKK291 pKa = 11.14KK292 pKa = 9.26LDD294 pKa = 3.41KK295 pKa = 11.2SVFSFDD301 pKa = 4.34LSAATDD307 pKa = 4.13RR308 pKa = 11.84LPMDD312 pKa = 3.3IQMVILNSLTPFWLAEE328 pKa = 3.75KK329 pKa = 10.26AVRR332 pKa = 11.84HH333 pKa = 5.04GVKK336 pKa = 10.43GGLGDD341 pKa = 3.64AWADD345 pKa = 3.57LLVDD349 pKa = 3.81RR350 pKa = 11.84DD351 pKa = 4.02YY352 pKa = 11.8YY353 pKa = 10.39LPRR356 pKa = 11.84WSGYY360 pKa = 10.65ARR362 pKa = 11.84DD363 pKa = 3.79SKK365 pKa = 11.13VRR367 pKa = 11.84YY368 pKa = 9.03AVGQPMGALSSWAMLALTHH387 pKa = 6.72HH388 pKa = 6.94MIVQFAAASVGVTGWFKK405 pKa = 11.06EE406 pKa = 3.79YY407 pKa = 9.9MVLGDD412 pKa = 5.78DD413 pKa = 3.29IVIYY417 pKa = 10.56NSEE420 pKa = 3.96VAKK423 pKa = 10.58AYY425 pKa = 7.24STLMGTLGVGISDD438 pKa = 3.93TKK440 pKa = 11.32SLTSKK445 pKa = 10.23IGVFEE450 pKa = 3.99FAKK453 pKa = 10.55RR454 pKa = 11.84LMDD457 pKa = 5.63LEE459 pKa = 5.37GPCQGLPLAEE469 pKa = 4.04FAAARR474 pKa = 11.84FNLSILFQSFRR485 pKa = 11.84SRR487 pKa = 11.84TLYY490 pKa = 10.25PKK492 pKa = 9.83ISTFMRR498 pKa = 11.84FLGFGYY504 pKa = 10.21KK505 pKa = 10.08VLGSLGMRR513 pKa = 11.84LGDD516 pKa = 3.25MRR518 pKa = 11.84KK519 pKa = 9.03RR520 pKa = 11.84SGYY523 pKa = 10.03FEE525 pKa = 4.19TVAYY529 pKa = 10.09SPLVTDD535 pKa = 4.13KK536 pKa = 11.25SVNKK540 pKa = 9.15WSEE543 pKa = 3.63FFKK546 pKa = 10.95RR547 pKa = 11.84FSTYY551 pKa = 10.99DD552 pKa = 3.68LYY554 pKa = 10.7PLVEE558 pKa = 4.11ACVYY562 pKa = 9.98KK563 pKa = 10.48AYY565 pKa = 10.71HH566 pKa = 7.23LIPALTKK573 pKa = 9.9FDD575 pKa = 3.9RR576 pKa = 11.84WNLWRR581 pKa = 11.84TLFPHH586 pKa = 7.01FDD588 pKa = 3.63DD589 pKa = 5.68KK590 pKa = 11.43KK591 pKa = 10.08ILLIPEE597 pKa = 3.75VAGRR601 pKa = 11.84HH602 pKa = 5.26LEE604 pKa = 4.12SLFTSMLDD612 pKa = 3.49NKK614 pKa = 8.67VAKK617 pKa = 10.24YY618 pKa = 10.22KK619 pKa = 10.4ADD621 pKa = 2.98HH622 pKa = 5.86SVYY625 pKa = 10.69VNRR628 pKa = 11.84LNRR631 pKa = 11.84AEE633 pKa = 4.32VPTNDD638 pKa = 3.2WEE640 pKa = 5.92AIDD643 pKa = 3.85TLLSIMEE650 pKa = 4.42TSPPTSDD657 pKa = 5.05LLQDD661 pKa = 3.59PDD663 pKa = 3.8MNEE666 pKa = 3.88LGFGLSVSNYY676 pKa = 10.82DD677 pKa = 3.89EE678 pKa = 4.99DD679 pKa = 4.6NKK681 pKa = 11.28SKK683 pKa = 9.13TCSLSLPYY691 pKa = 10.13TVFTKK696 pKa = 10.29IPQLLDD702 pKa = 4.64SIDD705 pKa = 3.9DD706 pKa = 3.6WVEE709 pKa = 3.61PIIPDD714 pKa = 3.25KK715 pKa = 11.01HH716 pKa = 6.99PEE718 pKa = 3.59VSADD722 pKa = 3.33VNRR725 pKa = 11.84PLIHH729 pKa = 6.83KK730 pKa = 8.61PGKK733 pKa = 10.42VFVKK737 pKa = 10.46GIATGKK743 pKa = 9.91NEE745 pKa = 3.99NNKK748 pKa = 9.43VEE750 pKa = 4.23ITKK753 pKa = 9.28MLQDD757 pKa = 4.09KK758 pKa = 10.71KK759 pKa = 10.17FLAYY763 pKa = 9.5IIEE766 pKa = 4.4SGKK769 pKa = 8.25ITIYY773 pKa = 10.87SPAFQAMIRR782 pKa = 11.84ASWAKK787 pKa = 9.89AQSGSKK793 pKa = 10.14

Molecular weight: 89.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6LFY6|F6LFY6_9VIRU RNA-dependent RNA polymerase OS=Tuber aestivum mitovirus OX=1037523 PE=4 SV=1
MM1 pKa = 7.36MNPFIRR7 pKa = 11.84LGSLLMGKK15 pKa = 6.16TTKK18 pKa = 10.47EE19 pKa = 4.05LIIGLYY25 pKa = 10.26SCVQTLHH32 pKa = 7.06KK33 pKa = 10.06IYY35 pKa = 10.37LSQGSRR41 pKa = 11.84GLAIRR46 pKa = 11.84LKK48 pKa = 10.79ASSTYY53 pKa = 11.03LMKK56 pKa = 10.5SWSGDD61 pKa = 3.37PLLDD65 pKa = 3.93CKK67 pKa = 11.2AFGPTMSLTPRR78 pKa = 11.84GIPIWIPSAWRR89 pKa = 11.84CQISSRR95 pKa = 11.84NLAIFRR101 pKa = 11.84VTMTLCNLYY110 pKa = 10.22RR111 pKa = 11.84VLPYY115 pKa = 10.07QGKK118 pKa = 9.84LKK120 pKa = 10.8LSTITDD126 pKa = 3.59DD127 pKa = 4.69RR128 pKa = 11.84KK129 pKa = 11.09GSTPDD134 pKa = 3.68LMFSFIKK141 pKa = 10.71DD142 pKa = 3.1KK143 pKa = 10.62FAPNITNRR151 pKa = 11.84VPYY154 pKa = 9.86PGIFSWDD161 pKa = 3.66PKK163 pKa = 10.32PLLTRR168 pKa = 11.84GPGAQKK174 pKa = 10.5EE175 pKa = 4.37KK176 pKa = 10.08NTMSGFGMALRR187 pKa = 11.84AIHH190 pKa = 5.34EE191 pKa = 4.43TGMLDD196 pKa = 3.51HH197 pKa = 6.92LKK199 pKa = 10.57AYY201 pKa = 10.49ALYY204 pKa = 10.01TGLNPQLEE212 pKa = 4.49SLKK215 pKa = 10.78VIWEE219 pKa = 4.43GTRR222 pKa = 11.84KK223 pKa = 9.49HH224 pKa = 6.48CSIGPIGKK232 pKa = 9.04LAFKK236 pKa = 10.41QEE238 pKa = 4.09PGKK241 pKa = 10.85VRR243 pKa = 11.84VFAMVDD249 pKa = 4.5CITQWFLHH257 pKa = 6.46PLHH260 pKa = 7.0KK261 pKa = 10.84YY262 pKa = 9.69LFSVLRR268 pKa = 11.84TTKK271 pKa = 10.5EE272 pKa = 3.65DD273 pKa = 3.2ATFDD277 pKa = 3.55QEE279 pKa = 4.6KK280 pKa = 10.45GINLVRR286 pKa = 11.84LALSKK291 pKa = 11.14KK292 pKa = 9.26LDD294 pKa = 3.41KK295 pKa = 11.2SVFSFDD301 pKa = 4.34LSAATDD307 pKa = 4.13RR308 pKa = 11.84LPMDD312 pKa = 3.3IQMVILNSLTPFWLAEE328 pKa = 3.75KK329 pKa = 10.26AVRR332 pKa = 11.84HH333 pKa = 5.04GVKK336 pKa = 10.43GGLGDD341 pKa = 3.64AWADD345 pKa = 3.57LLVDD349 pKa = 3.81RR350 pKa = 11.84DD351 pKa = 4.02YY352 pKa = 11.8YY353 pKa = 10.39LPRR356 pKa = 11.84WSGYY360 pKa = 10.65ARR362 pKa = 11.84DD363 pKa = 3.79SKK365 pKa = 11.13VRR367 pKa = 11.84YY368 pKa = 9.03AVGQPMGALSSWAMLALTHH387 pKa = 6.72HH388 pKa = 6.94MIVQFAAASVGVTGWFKK405 pKa = 11.06EE406 pKa = 3.79YY407 pKa = 9.9MVLGDD412 pKa = 5.78DD413 pKa = 3.29IVIYY417 pKa = 10.56NSEE420 pKa = 3.96VAKK423 pKa = 10.58AYY425 pKa = 7.24STLMGTLGVGISDD438 pKa = 3.93TKK440 pKa = 11.32SLTSKK445 pKa = 10.23IGVFEE450 pKa = 3.99FAKK453 pKa = 10.55RR454 pKa = 11.84LMDD457 pKa = 5.63LEE459 pKa = 5.37GPCQGLPLAEE469 pKa = 4.04FAAARR474 pKa = 11.84FNLSILFQSFRR485 pKa = 11.84SRR487 pKa = 11.84TLYY490 pKa = 10.25PKK492 pKa = 9.83ISTFMRR498 pKa = 11.84FLGFGYY504 pKa = 10.21KK505 pKa = 10.08VLGSLGMRR513 pKa = 11.84LGDD516 pKa = 3.25MRR518 pKa = 11.84KK519 pKa = 9.03RR520 pKa = 11.84SGYY523 pKa = 10.03FEE525 pKa = 4.19TVAYY529 pKa = 10.09SPLVTDD535 pKa = 4.13KK536 pKa = 11.25SVNKK540 pKa = 9.15WSEE543 pKa = 3.63FFKK546 pKa = 10.95RR547 pKa = 11.84FSTYY551 pKa = 10.99DD552 pKa = 3.68LYY554 pKa = 10.7PLVEE558 pKa = 4.11ACVYY562 pKa = 9.98KK563 pKa = 10.48AYY565 pKa = 10.71HH566 pKa = 7.23LIPALTKK573 pKa = 9.9FDD575 pKa = 3.9RR576 pKa = 11.84WNLWRR581 pKa = 11.84TLFPHH586 pKa = 7.01FDD588 pKa = 3.63DD589 pKa = 5.68KK590 pKa = 11.43KK591 pKa = 10.08ILLIPEE597 pKa = 3.75VAGRR601 pKa = 11.84HH602 pKa = 5.26LEE604 pKa = 4.12SLFTSMLDD612 pKa = 3.49NKK614 pKa = 8.67VAKK617 pKa = 10.24YY618 pKa = 10.22KK619 pKa = 10.4ADD621 pKa = 2.98HH622 pKa = 5.86SVYY625 pKa = 10.69VNRR628 pKa = 11.84LNRR631 pKa = 11.84AEE633 pKa = 4.32VPTNDD638 pKa = 3.2WEE640 pKa = 5.92AIDD643 pKa = 3.85TLLSIMEE650 pKa = 4.42TSPPTSDD657 pKa = 5.05LLQDD661 pKa = 3.59PDD663 pKa = 3.8MNEE666 pKa = 3.88LGFGLSVSNYY676 pKa = 10.82DD677 pKa = 3.89EE678 pKa = 4.99DD679 pKa = 4.6NKK681 pKa = 11.28SKK683 pKa = 9.13TCSLSLPYY691 pKa = 10.13TVFTKK696 pKa = 10.29IPQLLDD702 pKa = 4.64SIDD705 pKa = 3.9DD706 pKa = 3.6WVEE709 pKa = 3.61PIIPDD714 pKa = 3.25KK715 pKa = 11.01HH716 pKa = 6.99PEE718 pKa = 3.59VSADD722 pKa = 3.33VNRR725 pKa = 11.84PLIHH729 pKa = 6.83KK730 pKa = 8.61PGKK733 pKa = 10.42VFVKK737 pKa = 10.46GIATGKK743 pKa = 9.91NEE745 pKa = 3.99NNKK748 pKa = 9.43VEE750 pKa = 4.23ITKK753 pKa = 9.28MLQDD757 pKa = 4.09KK758 pKa = 10.71KK759 pKa = 10.17FLAYY763 pKa = 9.5IIEE766 pKa = 4.4SGKK769 pKa = 8.25ITIYY773 pKa = 10.87SPAFQAMIRR782 pKa = 11.84ASWAKK787 pKa = 9.89AQSGSKK793 pKa = 10.14

Molecular weight: 89.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

793

793

793

793.0

89.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.683 ± 0.0

1.135 ± 0.0

5.675 ± 0.0

3.657 ± 0.0

5.044 ± 0.0

6.557 ± 0.0

1.892 ± 0.0

5.801 ± 0.0

7.818 ± 0.0

11.475 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.405 ± 0.0

3.026 ± 0.0

5.296 ± 0.0

2.396 ± 0.0

4.792 ± 0.0

7.945 ± 0.0

5.927 ± 0.0

5.549 ± 0.0

2.144 ± 0.0

3.783 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski