Amino acid dipepetide frequency for Gordonia phage Sproutie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.308AlaAla: 18.308 ± 2.376
0.808AlaCys: 0.808 ± 0.261
8.279AlaAsp: 8.279 ± 0.967
9.087AlaGlu: 9.087 ± 0.817
2.625AlaPhe: 2.625 ± 0.503
9.356AlaGly: 9.356 ± 0.851
2.288AlaHis: 2.288 ± 0.434
7.135AlaIle: 7.135 ± 0.602
3.904AlaLys: 3.904 ± 0.46
8.885AlaLeu: 8.885 ± 1.007
3.635AlaMet: 3.635 ± 0.451
4.173AlaAsn: 4.173 ± 0.565
5.519AlaPro: 5.519 ± 0.648
4.644AlaGln: 4.644 ± 0.57
7.875AlaArg: 7.875 ± 0.744
7.067AlaSer: 7.067 ± 0.81
8.01AlaThr: 8.01 ± 1.051
7.808AlaVal: 7.808 ± 0.943
1.683AlaTrp: 1.683 ± 0.303
2.423AlaTyr: 2.423 ± 0.414
0.0AlaXaa: 0.0 ± 0.0
Cys
1.077CysAla: 1.077 ± 0.301
0.135CysCys: 0.135 ± 0.104
0.538CysAsp: 0.538 ± 0.275
0.538CysGlu: 0.538 ± 0.217
0.135CysPhe: 0.135 ± 0.088
0.875CysGly: 0.875 ± 0.383
0.135CysHis: 0.135 ± 0.094
0.067CysIle: 0.067 ± 0.067
0.202CysLys: 0.202 ± 0.111
0.404CysLeu: 0.404 ± 0.136
0.067CysMet: 0.067 ± 0.064
0.135CysAsn: 0.135 ± 0.1
0.471CysPro: 0.471 ± 0.182
0.269CysGln: 0.269 ± 0.161
0.875CysArg: 0.875 ± 0.268
0.606CysSer: 0.606 ± 0.217
0.538CysThr: 0.538 ± 0.173
0.404CysVal: 0.404 ± 0.161
0.269CysTrp: 0.269 ± 0.129
0.202CysTyr: 0.202 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
7.74AspAla: 7.74 ± 0.782
0.74AspCys: 0.74 ± 0.266
4.981AspAsp: 4.981 ± 0.684
4.51AspGlu: 4.51 ± 0.624
2.087AspPhe: 2.087 ± 0.366
6.462AspGly: 6.462 ± 0.655
1.548AspHis: 1.548 ± 0.389
2.692AspIle: 2.692 ± 0.457
1.413AspLys: 1.413 ± 0.239
7.135AspLeu: 7.135 ± 0.693
1.346AspMet: 1.346 ± 0.325
2.019AspAsn: 2.019 ± 0.455
5.048AspPro: 5.048 ± 0.517
1.885AspGln: 1.885 ± 0.341
4.981AspArg: 4.981 ± 0.59
2.76AspSer: 2.76 ± 0.407
3.971AspThr: 3.971 ± 0.479
4.51AspVal: 4.51 ± 0.487
1.548AspTrp: 1.548 ± 0.286
1.481AspTyr: 1.481 ± 0.282
0.0AspXaa: 0.0 ± 0.0
Glu
7.337GluAla: 7.337 ± 0.763
0.808GluCys: 0.808 ± 0.289
2.558GluAsp: 2.558 ± 0.47
2.827GluGlu: 2.827 ± 0.632
1.952GluPhe: 1.952 ± 0.375
4.106GluGly: 4.106 ± 0.562
1.144GluHis: 1.144 ± 0.354
3.298GluIle: 3.298 ± 0.494
1.817GluLys: 1.817 ± 0.334
5.519GluLeu: 5.519 ± 0.745
1.481GluMet: 1.481 ± 0.323
1.413GluAsn: 1.413 ± 0.276
3.365GluPro: 3.365 ± 0.456
3.231GluGln: 3.231 ± 0.574
5.25GluArg: 5.25 ± 0.603
3.5GluSer: 3.5 ± 0.58
3.365GluThr: 3.365 ± 0.544
2.827GluVal: 2.827 ± 0.406
0.808GluTrp: 0.808 ± 0.226
1.817GluTyr: 1.817 ± 0.41
0.0GluXaa: 0.0 ± 0.0
Phe
3.567PheAla: 3.567 ± 0.478
0.135PheCys: 0.135 ± 0.086
2.76PheAsp: 2.76 ± 0.529
1.346PheGlu: 1.346 ± 0.343
0.808PhePhe: 0.808 ± 0.235
3.096PheGly: 3.096 ± 0.62
0.269PheHis: 0.269 ± 0.159
0.808PheIle: 0.808 ± 0.221
0.673PheLys: 0.673 ± 0.209
1.481PheLeu: 1.481 ± 0.281
0.404PheMet: 0.404 ± 0.16
0.538PheAsn: 0.538 ± 0.209
0.942PhePro: 0.942 ± 0.257
0.538PheGln: 0.538 ± 0.173
1.75PheArg: 1.75 ± 0.332
1.346PheSer: 1.346 ± 0.257
2.288PheThr: 2.288 ± 0.412
1.885PheVal: 1.885 ± 0.403
1.481PheTrp: 1.481 ± 0.4
0.269PheTyr: 0.269 ± 0.148
0.0PheXaa: 0.0 ± 0.0
Gly
8.144GlyAla: 8.144 ± 0.909
0.337GlyCys: 0.337 ± 0.195
6.462GlyAsp: 6.462 ± 0.641
4.846GlyGlu: 4.846 ± 0.573
2.154GlyPhe: 2.154 ± 0.43
7.808GlyGly: 7.808 ± 1.289
1.548GlyHis: 1.548 ± 0.28
4.51GlyIle: 4.51 ± 0.518
3.567GlyLys: 3.567 ± 0.467
6.462GlyLeu: 6.462 ± 0.719
1.885GlyMet: 1.885 ± 0.395
2.894GlyAsn: 2.894 ± 0.51
4.106GlyPro: 4.106 ± 0.513
3.029GlyGln: 3.029 ± 0.501
6.798GlyArg: 6.798 ± 0.584
5.183GlySer: 5.183 ± 0.688
5.587GlyThr: 5.587 ± 0.82
6.462GlyVal: 6.462 ± 0.651
1.212GlyTrp: 1.212 ± 0.25
2.558GlyTyr: 2.558 ± 0.402
0.0GlyXaa: 0.0 ± 0.0
His
1.615HisAla: 1.615 ± 0.312
0.337HisCys: 0.337 ± 0.156
1.346HisAsp: 1.346 ± 0.285
1.077HisGlu: 1.077 ± 0.342
0.404HisPhe: 0.404 ± 0.173
1.817HisGly: 1.817 ± 0.48
0.673HisHis: 0.673 ± 0.208
0.673HisIle: 0.673 ± 0.219
0.606HisLys: 0.606 ± 0.202
1.885HisLeu: 1.885 ± 0.434
0.202HisMet: 0.202 ± 0.117
0.471HisAsn: 0.471 ± 0.192
1.548HisPro: 1.548 ± 0.332
0.942HisGln: 0.942 ± 0.254
1.01HisArg: 1.01 ± 0.285
0.606HisSer: 0.606 ± 0.236
1.144HisThr: 1.144 ± 0.228
0.942HisVal: 0.942 ± 0.26
0.673HisTrp: 0.673 ± 0.216
0.606HisTyr: 0.606 ± 0.212
0.0HisXaa: 0.0 ± 0.0
Ile
5.99IleAla: 5.99 ± 0.724
0.269IleCys: 0.269 ± 0.148
4.712IleAsp: 4.712 ± 0.494
3.029IleGlu: 3.029 ± 0.36
1.481IlePhe: 1.481 ± 0.404
4.712IleGly: 4.712 ± 0.437
0.942IleHis: 0.942 ± 0.263
1.615IleIle: 1.615 ± 0.332
1.144IleLys: 1.144 ± 0.329
3.096IleLeu: 3.096 ± 0.445
0.538IleMet: 0.538 ± 0.18
1.279IleAsn: 1.279 ± 0.341
2.356IlePro: 2.356 ± 0.356
2.49IleGln: 2.49 ± 0.355
3.567IleArg: 3.567 ± 0.425
2.894IleSer: 2.894 ± 0.365
3.298IleThr: 3.298 ± 0.424
4.24IleVal: 4.24 ± 0.499
0.337IleTrp: 0.337 ± 0.166
1.481IleTyr: 1.481 ± 0.33
0.0IleXaa: 0.0 ± 0.0
Lys
3.837LysAla: 3.837 ± 0.504
0.067LysCys: 0.067 ± 0.065
2.087LysAsp: 2.087 ± 0.398
1.279LysGlu: 1.279 ± 0.374
0.74LysPhe: 0.74 ± 0.261
2.154LysGly: 2.154 ± 0.412
0.538LysHis: 0.538 ± 0.196
1.212LysIle: 1.212 ± 0.258
0.74LysLys: 0.74 ± 0.288
2.962LysLeu: 2.962 ± 0.535
0.808LysMet: 0.808 ± 0.255
0.808LysAsn: 0.808 ± 0.221
1.952LysPro: 1.952 ± 0.366
1.144LysGln: 1.144 ± 0.285
2.087LysArg: 2.087 ± 0.375
1.817LysSer: 1.817 ± 0.402
1.952LysThr: 1.952 ± 0.356
2.558LysVal: 2.558 ± 0.349
0.538LysTrp: 0.538 ± 0.2
0.74LysTyr: 0.74 ± 0.261
0.0LysXaa: 0.0 ± 0.0
Leu
10.567LeuAla: 10.567 ± 0.995
0.673LeuCys: 0.673 ± 0.275
5.183LeuAsp: 5.183 ± 0.649
3.769LeuGlu: 3.769 ± 0.459
1.817LeuPhe: 1.817 ± 0.518
7.673LeuGly: 7.673 ± 0.767
1.212LeuHis: 1.212 ± 0.351
3.635LeuIle: 3.635 ± 0.611
1.952LeuLys: 1.952 ± 0.417
5.25LeuLeu: 5.25 ± 0.644
1.548LeuMet: 1.548 ± 0.32
2.019LeuAsn: 2.019 ± 0.349
4.981LeuPro: 4.981 ± 0.489
2.221LeuGln: 2.221 ± 0.417
5.519LeuArg: 5.519 ± 0.73
4.51LeuSer: 4.51 ± 0.502
5.587LeuThr: 5.587 ± 0.719
6.26LeuVal: 6.26 ± 0.83
1.212LeuTrp: 1.212 ± 0.272
1.413LeuTyr: 1.413 ± 0.256
0.0LeuXaa: 0.0 ± 0.0
Met
2.962MetAla: 2.962 ± 0.655
0.404MetCys: 0.404 ± 0.172
0.942MetAsp: 0.942 ± 0.226
0.808MetGlu: 0.808 ± 0.201
0.471MetPhe: 0.471 ± 0.157
1.615MetGly: 1.615 ± 0.41
0.337MetHis: 0.337 ± 0.167
1.346MetIle: 1.346 ± 0.331
0.538MetLys: 0.538 ± 0.198
1.75MetLeu: 1.75 ± 0.303
0.538MetMet: 0.538 ± 0.17
0.74MetAsn: 0.74 ± 0.251
1.279MetPro: 1.279 ± 0.267
0.606MetGln: 0.606 ± 0.22
1.346MetArg: 1.346 ± 0.305
1.75MetSer: 1.75 ± 0.309
2.356MetThr: 2.356 ± 0.415
0.942MetVal: 0.942 ± 0.215
0.269MetTrp: 0.269 ± 0.116
0.269MetTyr: 0.269 ± 0.09
0.0MetXaa: 0.0 ± 0.0
Asn
3.702AsnAla: 3.702 ± 0.547
0.067AsnCys: 0.067 ± 0.063
1.481AsnAsp: 1.481 ± 0.337
1.413AsnGlu: 1.413 ± 0.307
0.875AsnPhe: 0.875 ± 0.402
2.76AsnGly: 2.76 ± 0.487
0.673AsnHis: 0.673 ± 0.364
1.346AsnIle: 1.346 ± 0.326
0.942AsnLys: 0.942 ± 0.24
1.885AsnLeu: 1.885 ± 0.346
0.404AsnMet: 0.404 ± 0.167
0.606AsnAsn: 0.606 ± 0.155
2.221AsnPro: 2.221 ± 0.431
1.279AsnGln: 1.279 ± 0.354
2.558AsnArg: 2.558 ± 0.502
1.615AsnSer: 1.615 ± 0.303
1.952AsnThr: 1.952 ± 0.424
1.481AsnVal: 1.481 ± 0.271
0.404AsnTrp: 0.404 ± 0.178
0.875AsnTyr: 0.875 ± 0.214
0.0AsnXaa: 0.0 ± 0.0
Pro
7.337ProAla: 7.337 ± 0.862
0.471ProCys: 0.471 ± 0.198
4.914ProAsp: 4.914 ± 0.716
4.577ProGlu: 4.577 ± 0.638
0.875ProPhe: 0.875 ± 0.234
5.183ProGly: 5.183 ± 0.554
1.144ProHis: 1.144 ± 0.26
2.49ProIle: 2.49 ± 0.399
1.817ProLys: 1.817 ± 0.383
3.298ProLeu: 3.298 ± 0.501
1.077ProMet: 1.077 ± 0.243
1.952ProAsn: 1.952 ± 0.379
3.365ProPro: 3.365 ± 0.459
1.615ProGln: 1.615 ± 0.236
4.106ProArg: 4.106 ± 0.685
2.692ProSer: 2.692 ± 0.39
3.5ProThr: 3.5 ± 0.748
3.635ProVal: 3.635 ± 0.522
1.481ProTrp: 1.481 ± 0.316
1.01ProTyr: 1.01 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
3.971GlnAla: 3.971 ± 0.604
0.269GlnCys: 0.269 ± 0.127
1.615GlnAsp: 1.615 ± 0.3
1.413GlnGlu: 1.413 ± 0.277
1.144GlnPhe: 1.144 ± 0.318
2.019GlnGly: 2.019 ± 0.355
0.808GlnHis: 0.808 ± 0.251
2.76GlnIle: 2.76 ± 0.419
1.212GlnLys: 1.212 ± 0.325
2.76GlnLeu: 2.76 ± 0.37
1.615GlnMet: 1.615 ± 0.29
1.077GlnAsn: 1.077 ± 0.279
2.087GlnPro: 2.087 ± 0.337
2.087GlnGln: 2.087 ± 0.528
3.567GlnArg: 3.567 ± 0.77
1.885GlnSer: 1.885 ± 0.315
2.49GlnThr: 2.49 ± 0.389
2.962GlnVal: 2.962 ± 0.368
0.808GlnTrp: 0.808 ± 0.254
0.606GlnTyr: 0.606 ± 0.243
0.0GlnXaa: 0.0 ± 0.0
Arg
7.74ArgAla: 7.74 ± 0.667
0.606ArgCys: 0.606 ± 0.281
3.904ArgAsp: 3.904 ± 0.533
4.914ArgGlu: 4.914 ± 0.708
2.625ArgPhe: 2.625 ± 0.494
4.846ArgGly: 4.846 ± 0.607
1.144ArgHis: 1.144 ± 0.33
4.375ArgIle: 4.375 ± 0.536
3.231ArgLys: 3.231 ± 0.487
5.923ArgLeu: 5.923 ± 0.683
1.817ArgMet: 1.817 ± 0.328
2.49ArgAsn: 2.49 ± 0.326
4.173ArgPro: 4.173 ± 0.66
2.558ArgGln: 2.558 ± 0.471
6.462ArgArg: 6.462 ± 0.965
4.039ArgSer: 4.039 ± 0.58
4.981ArgThr: 4.981 ± 0.626
5.856ArgVal: 5.856 ± 0.571
1.615ArgTrp: 1.615 ± 0.355
1.817ArgTyr: 1.817 ± 0.43
0.0ArgXaa: 0.0 ± 0.0
Ser
7.269SerAla: 7.269 ± 0.83
0.471SerCys: 0.471 ± 0.156
3.163SerAsp: 3.163 ± 0.469
2.827SerGlu: 2.827 ± 0.378
1.75SerPhe: 1.75 ± 0.325
6.125SerGly: 6.125 ± 0.787
0.606SerHis: 0.606 ± 0.212
3.096SerIle: 3.096 ± 0.413
1.615SerLys: 1.615 ± 0.311
4.577SerLeu: 4.577 ± 0.599
0.673SerMet: 0.673 ± 0.242
1.279SerAsn: 1.279 ± 0.264
3.365SerPro: 3.365 ± 0.588
2.019SerGln: 2.019 ± 0.364
3.702SerArg: 3.702 ± 0.579
2.827SerSer: 2.827 ± 0.461
3.635SerThr: 3.635 ± 0.482
3.365SerVal: 3.365 ± 0.485
1.817SerTrp: 1.817 ± 0.362
1.144SerTyr: 1.144 ± 0.248
0.0SerXaa: 0.0 ± 0.0
Thr
9.49ThrAla: 9.49 ± 1.57
0.471ThrCys: 0.471 ± 0.169
4.644ThrAsp: 4.644 ± 0.686
3.635ThrGlu: 3.635 ± 0.479
1.144ThrPhe: 1.144 ± 0.297
6.26ThrGly: 6.26 ± 0.854
1.077ThrHis: 1.077 ± 0.312
4.577ThrIle: 4.577 ± 0.576
1.413ThrLys: 1.413 ± 0.34
5.519ThrLeu: 5.519 ± 0.535
0.942ThrMet: 0.942 ± 0.304
1.817ThrAsn: 1.817 ± 0.436
3.5ThrPro: 3.5 ± 0.484
2.087ThrGln: 2.087 ± 0.378
3.837ThrArg: 3.837 ± 0.453
4.24ThrSer: 4.24 ± 0.647
4.779ThrThr: 4.779 ± 0.754
5.519ThrVal: 5.519 ± 0.774
1.481ThrTrp: 1.481 ± 0.297
0.875ThrTyr: 0.875 ± 0.208
0.0ThrXaa: 0.0 ± 0.0
Val
8.414ValAla: 8.414 ± 0.844
0.606ValCys: 0.606 ± 0.248
6.731ValAsp: 6.731 ± 0.588
4.308ValGlu: 4.308 ± 0.453
2.221ValPhe: 2.221 ± 0.431
5.048ValGly: 5.048 ± 0.695
1.144ValHis: 1.144 ± 0.308
2.962ValIle: 2.962 ± 0.515
1.885ValLys: 1.885 ± 0.32
4.51ValLeu: 4.51 ± 0.61
1.413ValMet: 1.413 ± 0.295
1.952ValAsn: 1.952 ± 0.422
4.039ValPro: 4.039 ± 0.554
2.558ValGln: 2.558 ± 0.457
6.192ValArg: 6.192 ± 0.646
3.904ValSer: 3.904 ± 0.651
5.048ValThr: 5.048 ± 0.713
6.327ValVal: 6.327 ± 0.874
1.077ValTrp: 1.077 ± 0.304
1.212ValTyr: 1.212 ± 0.303
0.0ValXaa: 0.0 ± 0.0
Trp
1.952TrpAla: 1.952 ± 0.385
0.202TrpCys: 0.202 ± 0.11
1.279TrpAsp: 1.279 ± 0.355
1.01TrpGlu: 1.01 ± 0.222
0.74TrpPhe: 0.74 ± 0.263
1.683TrpGly: 1.683 ± 0.354
0.404TrpHis: 0.404 ± 0.161
0.404TrpIle: 0.404 ± 0.163
0.404TrpLys: 0.404 ± 0.15
2.49TrpLeu: 2.49 ± 0.432
0.606TrpMet: 0.606 ± 0.201
0.471TrpAsn: 0.471 ± 0.172
1.212TrpPro: 1.212 ± 0.321
0.808TrpGln: 0.808 ± 0.208
1.885TrpArg: 1.885 ± 0.326
1.144TrpSer: 1.144 ± 0.254
1.212TrpThr: 1.212 ± 0.261
1.077TrpVal: 1.077 ± 0.228
0.74TrpTrp: 0.74 ± 0.241
0.269TrpTyr: 0.269 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.49TyrAla: 2.49 ± 0.488
0.067TyrCys: 0.067 ± 0.074
1.279TyrAsp: 1.279 ± 0.353
1.212TyrGlu: 1.212 ± 0.387
0.337TyrPhe: 0.337 ± 0.121
1.952TyrGly: 1.952 ± 0.37
0.942TyrHis: 0.942 ± 0.294
0.471TyrIle: 0.471 ± 0.141
0.942TyrLys: 0.942 ± 0.24
1.279TyrLeu: 1.279 ± 0.253
0.202TyrMet: 0.202 ± 0.095
0.404TyrAsn: 0.404 ± 0.154
1.01TyrPro: 1.01 ± 0.281
1.144TyrGln: 1.144 ± 0.317
1.75TyrArg: 1.75 ± 0.414
0.942TyrSer: 0.942 ± 0.208
1.615TyrThr: 1.615 ± 0.293
2.49TyrVal: 2.49 ± 0.39
0.538TyrTrp: 0.538 ± 0.188
0.404TyrTyr: 0.404 ± 0.159
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (14858 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski