Nocardioides euryhalodurans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3773 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P7GLC7|A0A4P7GLC7_9ACTN Thioredoxin OS=Nocardioides euryhalodurans OX=2518370 GN=trxA PE=3 SV=1
MM1 pKa = 6.51TRR3 pKa = 11.84SRR5 pKa = 11.84ITSTACILASAALVTTGCAGPTPASEE31 pKa = 4.34GGEE34 pKa = 4.06WTPEE38 pKa = 3.43GDD40 pKa = 3.19MTMVVPFAAGGGSDD54 pKa = 3.37LSGRR58 pKa = 11.84AMAAGLEE65 pKa = 4.38GVQDD69 pKa = 3.99GLNVAVEE76 pKa = 4.18NRR78 pKa = 11.84DD79 pKa = 3.51GASGAVGYY87 pKa = 10.68SYY89 pKa = 11.35FLTKK93 pKa = 10.55EE94 pKa = 3.52GDD96 pKa = 3.56PNYY99 pKa = 10.79VLATEE104 pKa = 4.32TALLALPLTQDD115 pKa = 3.22VEE117 pKa = 4.21FDD119 pKa = 3.77YY120 pKa = 11.52TSFTPIMKK128 pKa = 10.04VGEE131 pKa = 4.61DD132 pKa = 3.5FTLLVVDD139 pKa = 4.83ADD141 pKa = 4.43SPYY144 pKa = 9.13EE145 pKa = 4.01TCTDD149 pKa = 3.24VVDD152 pKa = 3.73AAKK155 pKa = 10.25SEE157 pKa = 4.11RR158 pKa = 11.84VIAGISGEE166 pKa = 4.23TGLDD170 pKa = 3.06NVVFTLTEE178 pKa = 3.57QQMDD182 pKa = 3.84VEE184 pKa = 4.69FDD186 pKa = 3.39RR187 pKa = 11.84VPFEE191 pKa = 4.45SGSEE195 pKa = 3.81LTAALLGGTVDD206 pKa = 3.73IASLNPGEE214 pKa = 5.06VIGQLEE220 pKa = 4.25SGDD223 pKa = 3.61IKK225 pKa = 11.14ALCAYY230 pKa = 10.23ADD232 pKa = 3.44EE233 pKa = 5.65RR234 pKa = 11.84YY235 pKa = 10.25DD236 pKa = 3.78YY237 pKa = 10.87PEE239 pKa = 5.15LSDD242 pKa = 3.79IPTAKK247 pKa = 10.21EE248 pKa = 3.39QGIDD252 pKa = 3.31VSFAQFRR259 pKa = 11.84GLLATGGITEE269 pKa = 4.5SEE271 pKa = 3.96KK272 pKa = 10.88QGWIEE277 pKa = 3.76AAEE280 pKa = 4.16AFADD284 pKa = 3.4SDD286 pKa = 3.95AYY288 pKa = 9.79TEE290 pKa = 4.31YY291 pKa = 11.3VEE293 pKa = 5.33SNYY296 pKa = 9.95LQPVTAFGDD305 pKa = 3.79DD306 pKa = 3.47FTSYY310 pKa = 11.12LEE312 pKa = 4.52SNSEE316 pKa = 3.97TLAGVLEE323 pKa = 4.36

Molecular weight: 33.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P7GKJ7|A0A4P7GKJ7_9ACTN Multidrug efflux SMR transporter OS=Nocardioides euryhalodurans OX=2518370 GN=EXE57_09890 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3773

0

3773

1218950

11

3629

323.1

34.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.892 ± 0.056

0.72 ± 0.011

6.622 ± 0.037

6.07 ± 0.037

2.757 ± 0.022

9.39 ± 0.039

2.245 ± 0.022

3.12 ± 0.025

1.622 ± 0.026

10.519 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.831 ± 0.016

1.576 ± 0.021

5.632 ± 0.032

2.723 ± 0.019

7.971 ± 0.049

5.098 ± 0.023

6.054 ± 0.033

9.69 ± 0.037

1.569 ± 0.017

1.899 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski