Streptococcus phage Javan226
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AZ58|A0A4D6AZ58_9CAUD Uncharacterized protein OS=Streptococcus phage Javan226 OX=2548055 GN=Javan226_0033 PE=4 SV=1
MM1 pKa = 7.88 TIPKK5 pKa = 9.57 FRR7 pKa = 11.84 VYY9 pKa = 11.1 DD10 pKa = 3.47 KK11 pKa = 10.83 VEE13 pKa = 4.16 RR14 pKa = 11.84 MMITTSDD21 pKa = 3.91 YY22 pKa = 11.53 EE23 pKa = 4.16 DD24 pKa = 5.06 LSDD27 pKa = 5.21 LFCFLKK33 pKa = 10.78 ADD35 pKa = 4.27 ADD37 pKa = 3.6 TGYY40 pKa = 10.83 YY41 pKa = 9.49 SEE43 pKa = 5.61 LMQSTGLYY51 pKa = 7.91 TVPLCPGLDD60 pKa = 3.66 YY61 pKa = 10.85 EE62 pKa = 4.53 RR63 pKa = 11.84 KK64 pKa = 10.0 EE65 pKa = 3.92 IFEE68 pKa = 4.2 GDD70 pKa = 3.21 ILKK73 pKa = 9.71 WYY75 pKa = 8.68 YY76 pKa = 10.99 SEE78 pKa = 5.92 GIEE81 pKa = 4.53 LQQQGTFVVVFEE93 pKa = 4.76 NGAFRR98 pKa = 11.84 PNKK101 pKa = 10.33 GNDD104 pKa = 3.05 ITLHH108 pKa = 6.34 EE109 pKa = 4.49 MLEE112 pKa = 4.35 DD113 pKa = 4.32 CDD115 pKa = 3.63 WAEE118 pKa = 4.46 VIGNLYY124 pKa = 9.8 EE125 pKa = 4.19 NPEE128 pKa = 3.83 ILEE131 pKa = 4.06 EE132 pKa = 4.41 LEE134 pKa = 4.03
Molecular weight: 15.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.817
IPC2_protein 4.062
IPC_protein 3.999
Toseland 3.821
ProMoST 4.088
Dawson 3.961
Bjellqvist 4.151
Wikipedia 3.834
Rodwell 3.834
Grimsley 3.732
Solomon 3.948
Lehninger 3.897
Nozaki 4.075
DTASelect 4.215
Thurlkill 3.846
EMBOSS 3.859
Sillero 4.113
Patrickios 1.074
IPC_peptide 3.948
IPC2_peptide 4.101
IPC2.peptide.svr19 4.017
Protein with the highest isoelectric point:
>tr|A0A4D6AUK2|A0A4D6AUK2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan226 OX=2548055 GN=Javan226_0012 PE=4 SV=1
MM1 pKa = 7.5 VINSNDD7 pKa = 3.3 RR8 pKa = 11.84 FHH10 pKa = 6.99 RR11 pKa = 11.84 QAQAVMTRR19 pKa = 11.84 RR20 pKa = 11.84 IKK22 pKa = 10.54 ALAMYY27 pKa = 8.98 EE28 pKa = 4.15 VTAKK32 pKa = 10.12 KK33 pKa = 10.14 DD34 pKa = 3.39 KK35 pKa = 10.51 QMKK38 pKa = 8.88 PFSPEE43 pKa = 3.97 RR44 pKa = 11.84 PCHH47 pKa = 6.81 LIVTVYY53 pKa = 10.74 KK54 pKa = 8.57 PTNRR58 pKa = 11.84 RR59 pKa = 11.84 LDD61 pKa = 3.66 TPNLYY66 pKa = 8.39 PTVKK70 pKa = 10.53 ALVDD74 pKa = 3.55 GMTEE78 pKa = 3.96 AKK80 pKa = 9.77 IWTDD84 pKa = 4.01 DD85 pKa = 3.4 NDD87 pKa = 3.75 NVIKK91 pKa = 9.64 STKK94 pKa = 8.97 FQLGGLSGKK103 pKa = 9.62 KK104 pKa = 9.49 GYY106 pKa = 10.32 YY107 pKa = 9.6 RR108 pKa = 11.84 FVLTVEE114 pKa = 4.5 EE115 pKa = 4.55 VV116 pKa = 2.92
Molecular weight: 13.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.028
IPC2_protein 9.165
IPC_protein 9.107
Toseland 9.911
ProMoST 9.56
Dawson 10.101
Bjellqvist 9.75
Wikipedia 10.248
Rodwell 10.584
Grimsley 10.16
Solomon 10.131
Lehninger 10.101
Nozaki 9.897
DTASelect 9.736
Thurlkill 9.955
EMBOSS 10.306
Sillero 10.014
Patrickios 10.218
IPC_peptide 10.131
IPC2_peptide 8.273
IPC2.peptide.svr19 8.088
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
11371
48
1523
214.5
24.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.976 ± 0.862
0.413 ± 0.086
5.98 ± 0.314
7.317 ± 0.74
3.931 ± 0.269
6.191 ± 0.49
1.161 ± 0.176
6.947 ± 0.301
8.311 ± 0.566
7.783 ± 0.234
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.506 ± 0.232
5.998 ± 0.499
2.55 ± 0.17
4.327 ± 0.285
3.395 ± 0.231
6.842 ± 0.602
6.657 ± 0.651
6.561 ± 0.264
0.967 ± 0.139
4.186 ± 0.465
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here