Legionella sp. TUM19329
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3144 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6F8T433|A0A6F8T433_9GAMM Glutamate--tRNA ligase OS=Legionella sp. TUM19329 OX=2708020 GN=gltX PE=3 SV=1
MM1 pKa = 6.69 VTISNSTISANLVHH15 pKa = 6.91 GSFVAGVLNNNSTEE29 pKa = 4.15 GLGKK33 pKa = 9.36 GTVSITGSTITVNADD48 pKa = 3.18 DD49 pKa = 4.94 AGLAGGVFNQANNPNGISSNINIDD73 pKa = 3.22 QSAISVTSNNNGGGTAAGVLTSGNGTFDD101 pKa = 4.25 FNNSSINVAGNSGSIAGVVVGDD123 pKa = 3.95 PTATANLQDD132 pKa = 4.21 TIISLVTSGTAVGAPIINGGTLNDD156 pKa = 3.86 NGGNQCFQNGAPVPCC171 pKa = 5.1
Molecular weight: 16.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.821
IPC2_protein 3.605
IPC_protein 3.503
Toseland 3.3
ProMoST 3.757
Dawson 3.554
Bjellqvist 3.719
Wikipedia 3.592
Rodwell 3.35
Grimsley 3.223
Solomon 3.478
Lehninger 3.439
Nozaki 3.706
DTASelect 3.961
Thurlkill 3.414
EMBOSS 3.579
Sillero 3.643
Patrickios 1.863
IPC_peptide 3.478
IPC2_peptide 3.592
IPC2.peptide.svr19 3.68
Protein with the highest isoelectric point:
>tr|A0A6F8T2W0|A0A6F8T2W0_9GAMM Tryptophan/tyrosine permease OS=Legionella sp. TUM19329 OX=2708020 GN=TUM19329_08680 PE=4 SV=1
MM1 pKa = 7.16 KK2 pKa = 7.76 TTSRR6 pKa = 11.84 TEE8 pKa = 3.56 KK9 pKa = 10.38 KK10 pKa = 10.49 LYY12 pKa = 10.86 SFDD15 pKa = 3.42 SAPRR19 pKa = 11.84 CGARR23 pKa = 11.84 TKK25 pKa = 10.99 SNNGEE30 pKa = 4.11 PCRR33 pKa = 11.84 CPAIRR38 pKa = 11.84 GKK40 pKa = 9.57 SRR42 pKa = 11.84 CRR44 pKa = 11.84 VHH46 pKa = 7.16 GGARR50 pKa = 11.84 GSGAPHH56 pKa = 6.39 YY57 pKa = 10.35 NLNALKK63 pKa = 10.37 HH64 pKa = 5.97 GYY66 pKa = 6.18 TTGEE70 pKa = 3.54 AKK72 pKa = 10.55 AFRR75 pKa = 11.84 EE76 pKa = 4.47 DD77 pKa = 2.58 IRR79 pKa = 11.84 LAIKK83 pKa = 10.3 RR84 pKa = 11.84 SNRR87 pKa = 11.84 FIKK90 pKa = 10.52 EE91 pKa = 3.92 LVV93 pKa = 3.23
Molecular weight: 10.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 9.648
IPC_protein 10.16
Toseland 10.687
ProMoST 10.277
Dawson 10.774
Bjellqvist 10.438
Wikipedia 10.935
Rodwell 11.125
Grimsley 10.804
Solomon 10.862
Lehninger 10.833
Nozaki 10.687
DTASelect 10.423
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.701
Patrickios 10.862
IPC_peptide 10.862
IPC2_peptide 9.531
IPC2.peptide.svr19 8.56
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3144
0
3144
953758
39
4261
303.4
34.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.483 ± 0.042
1.188 ± 0.017
4.98 ± 0.031
5.962 ± 0.05
4.322 ± 0.033
6.009 ± 0.043
2.512 ± 0.023
7.376 ± 0.042
6.406 ± 0.05
10.873 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.447 ± 0.022
4.992 ± 0.034
3.989 ± 0.027
4.593 ± 0.039
4.293 ± 0.032
6.736 ± 0.031
5.377 ± 0.039
5.954 ± 0.038
1.129 ± 0.017
3.382 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here