Bat Paramyxovirus Eid_hel/GH-M74a/GHA/2009
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I0E090|I0E090_9MONO Uncharacterized protein OS=Bat Paramyxovirus Eid_hel/GH-M74a/GHA/2009 OX=1112631 GN=C PE=4 SV=1
MM1 pKa = 7.94 DD2 pKa = 5.25 KK3 pKa = 10.97 EE4 pKa = 4.41 SLVDD8 pKa = 3.57 NGIKK12 pKa = 9.82 IIEE15 pKa = 4.93 FIQKK19 pKa = 10.15 NKK21 pKa = 11.08 DD22 pKa = 3.7 EE23 pKa = 4.19 IQKK26 pKa = 9.59 TYY28 pKa = 10.63 GRR30 pKa = 11.84 SQIQEE35 pKa = 3.5 PRR37 pKa = 11.84 TRR39 pKa = 11.84 EE40 pKa = 3.36 RR41 pKa = 11.84 AAAWEE46 pKa = 3.91 LFIRR50 pKa = 11.84 GEE52 pKa = 4.11 NPRR55 pKa = 11.84 PEE57 pKa = 4.81 GDD59 pKa = 2.96 RR60 pKa = 11.84 MAGVDD65 pKa = 3.44 QQDD68 pKa = 3.46 PTEE71 pKa = 4.41 RR72 pKa = 11.84 RR73 pKa = 11.84 DD74 pKa = 3.57 DD75 pKa = 4.01 DD76 pKa = 3.98 GRR78 pKa = 11.84 PNTLARR84 pKa = 11.84 DD85 pKa = 4.04 GEE87 pKa = 4.38 IGEE90 pKa = 4.68 SGPNYY95 pKa = 9.84 ISWSSGGDD103 pKa = 3.84 DD104 pKa = 3.33 IQLGPMVQDD113 pKa = 5.5 FEE115 pKa = 6.01 IDD117 pKa = 3.51 DD118 pKa = 4.18 NGIKK122 pKa = 10.43 LPNSRR127 pKa = 11.84 GSKK130 pKa = 9.04 SAPRR134 pKa = 11.84 CRR136 pKa = 11.84 SVDD139 pKa = 3.15 KK140 pKa = 11.16 SRR142 pKa = 11.84 ASDD145 pKa = 3.53 WEE147 pKa = 3.91 RR148 pKa = 11.84 SPASNIDD155 pKa = 3.38 HH156 pKa = 7.3 LSNLSGGGKK165 pKa = 10.04 DD166 pKa = 3.06 LGNYY170 pKa = 8.22 IKK172 pKa = 10.81 NVGVMVYY179 pKa = 9.25 PPTGAGVDD187 pKa = 3.7 RR188 pKa = 11.84 NSLTKK193 pKa = 9.84 PVSGVRR199 pKa = 11.84 QSRR202 pKa = 11.84 LSKK205 pKa = 9.65 LTWTKK210 pKa = 10.65 EE211 pKa = 4.09 SPLDD215 pKa = 3.59 QPEE218 pKa = 4.5 TVPEE222 pKa = 4.25 TLNDD226 pKa = 3.41 AYY228 pKa = 10.85 VIKK231 pKa = 10.31 EE232 pKa = 3.92 ATSKK236 pKa = 11.27 KK237 pKa = 10.17 EE238 pKa = 3.72 VTPEE242 pKa = 3.84 DD243 pKa = 4.74 GISILNPNAASFTPRR258 pKa = 11.84 STPAPKK264 pKa = 10.19 SVEE267 pKa = 3.87 KK268 pKa = 10.6 EE269 pKa = 3.81 PNQRR273 pKa = 11.84 QPAGGHH279 pKa = 6.52 PEE281 pKa = 4.59 DD282 pKa = 4.45 KK283 pKa = 10.1 GTPNRR288 pKa = 11.84 SEE290 pKa = 4.04 NPTTKK295 pKa = 9.45 STAIKK300 pKa = 9.63 NSKK303 pKa = 10.27 VITKK307 pKa = 10.63 AMVHH311 pKa = 5.97 TSPNEE316 pKa = 3.68 INQSARR322 pKa = 11.84 SMNIEE327 pKa = 4.63 FEE329 pKa = 4.49 PKK331 pKa = 10.25 SNPSTLTLEE340 pKa = 4.45 TVQPSNQQTNNTPASHH356 pKa = 7.41 PSGKK360 pKa = 6.65 TTRR363 pKa = 11.84 EE364 pKa = 4.08 LPKK367 pKa = 10.47 GAQPQSGTSRR377 pKa = 11.84 AANIKK382 pKa = 10.76 DD383 pKa = 3.41 PTTAPNKK390 pKa = 9.8 TSIQTKK396 pKa = 7.43 PARR399 pKa = 11.84 NASGGDD405 pKa = 3.34 GTNPQMASPPPPPVVTNPPLKK426 pKa = 10.48 KK427 pKa = 9.83 RR428 pKa = 11.84 VPKK431 pKa = 10.57 LSEE434 pKa = 4.21 PNTRR438 pKa = 11.84 DD439 pKa = 3.65 HH440 pKa = 7.1 LSHH443 pKa = 6.69 PTDD446 pKa = 3.26 EE447 pKa = 4.82 KK448 pKa = 10.8 RR449 pKa = 11.84 DD450 pKa = 3.66 HH451 pKa = 6.87 GGPAVKK457 pKa = 10.3 SKK459 pKa = 8.58 TQAHH463 pKa = 6.72 RR464 pKa = 11.84 GLNADD469 pKa = 3.51 NTQNCTDD476 pKa = 3.41 QPTPSSNKK484 pKa = 8.99 AQPPKK489 pKa = 10.05 TPKK492 pKa = 10.12 PEE494 pKa = 4.0 NPQAEE499 pKa = 4.75 PKK501 pKa = 9.2 PKK503 pKa = 10.2 KK504 pKa = 9.77 HH505 pKa = 6.5 VSFPNLDD512 pKa = 3.43 LTIEE516 pKa = 4.34 EE517 pKa = 4.43 EE518 pKa = 4.21 GGTAVDD524 pKa = 3.14 IMVNEE529 pKa = 4.17 TRR531 pKa = 11.84 EE532 pKa = 4.12 GVIDD536 pKa = 3.27 VHH538 pKa = 7.41 LIKK541 pKa = 10.58 EE542 pKa = 4.22 DD543 pKa = 3.71 NKK545 pKa = 10.34 ILNMDD550 pKa = 3.6 QDD552 pKa = 4.58 NPCSNQITSQQEE564 pKa = 3.81 TGVTKK569 pKa = 10.61 RR570 pKa = 11.84 GIGEE574 pKa = 3.94 NSSCIGTKK582 pKa = 9.73 EE583 pKa = 4.76 DD584 pKa = 3.45 SRR586 pKa = 11.84 QLSGAIQSAQKK597 pKa = 10.52 SRR599 pKa = 11.84 LSQRR603 pKa = 11.84 QEE605 pKa = 3.6 NASAEE610 pKa = 4.31 SARR613 pKa = 11.84 PGVTSVPSDD622 pKa = 3.48 DD623 pKa = 4.41 PEE625 pKa = 4.8 SEE627 pKa = 4.06 EE628 pKa = 4.05 VKK630 pKa = 10.05 TYY632 pKa = 10.26 RR633 pKa = 11.84 ASDD636 pKa = 4.16 AIIEE640 pKa = 4.01 QLNNDD645 pKa = 3.92 EE646 pKa = 4.4 AQDD649 pKa = 3.96 YY650 pKa = 11.3 YY651 pKa = 11.95 SFLDD655 pKa = 3.79 LMEE658 pKa = 4.64 NTDD661 pKa = 5.61 DD662 pKa = 5.85 DD663 pKa = 5.44 DD664 pKa = 5.81 LICEE668 pKa = 4.11 EE669 pKa = 4.82 TKK671 pKa = 10.71 FSLLNTARR679 pKa = 11.84 LISLNSRR686 pKa = 11.84 IEE688 pKa = 4.24 KK689 pKa = 10.06 IEE691 pKa = 3.92 EE692 pKa = 3.99 QIKK695 pKa = 10.21 KK696 pKa = 9.49 IPAMEE701 pKa = 4.6 KK702 pKa = 10.18 KK703 pKa = 10.69 LSDD706 pKa = 3.69 IEE708 pKa = 4.32 KK709 pKa = 10.79 LLLKK713 pKa = 10.06 TNTALSTIEE722 pKa = 3.82 GHH724 pKa = 5.73 ITSMMIMVPGKK735 pKa = 8.11 TVNEE739 pKa = 4.11 GEE741 pKa = 4.16 IQINEE746 pKa = 3.91 QLKK749 pKa = 9.89 PVIGRR754 pKa = 11.84 KK755 pKa = 9.93 DD756 pKa = 3.35 GVYY759 pKa = 10.98 DD760 pKa = 3.97 KK761 pKa = 10.6 MILPRR766 pKa = 11.84 VDD768 pKa = 3.45 KK769 pKa = 10.75 KK770 pKa = 10.97 ISLVTDD776 pKa = 4.14 RR777 pKa = 11.84 TPLCDD782 pKa = 4.89 DD783 pKa = 4.45 FKK785 pKa = 11.22 EE786 pKa = 4.39 DD787 pKa = 4.46 PYY789 pKa = 11.85 VLNEE793 pKa = 4.28 KK794 pKa = 10.59 LILDD798 pKa = 5.27 PINPSVTNASAFIPSYY814 pKa = 11.43 DD815 pKa = 3.9 NISPAVLRR823 pKa = 11.84 SLIRR827 pKa = 11.84 SNVEE831 pKa = 3.52 DD832 pKa = 3.74 RR833 pKa = 11.84 EE834 pKa = 4.44 TRR836 pKa = 11.84 VEE838 pKa = 4.69 LIEE841 pKa = 5.06 LVNQARR847 pKa = 11.84 NDD849 pKa = 3.71 SEE851 pKa = 4.76 LNEE854 pKa = 3.95 ILALVNDD861 pKa = 5.38 IIDD864 pKa = 4.05 SNQSGVV870 pKa = 3.08
Molecular weight: 95.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.271
IPC2_protein 5.245
IPC_protein 5.207
Toseland 5.156
ProMoST 5.347
Dawson 5.207
Bjellqvist 5.334
Wikipedia 5.067
Rodwell 5.118
Grimsley 5.08
Solomon 5.207
Lehninger 5.156
Nozaki 5.321
DTASelect 5.486
Thurlkill 5.156
EMBOSS 5.118
Sillero 5.397
Patrickios 4.609
IPC_peptide 5.207
IPC2_peptide 5.397
IPC2.peptide.svr19 5.401
Protein with the highest isoelectric point:
>tr|I0E092|I0E092_9MONO Fusion glycoprotein F0 OS=Bat Paramyxovirus Eid_hel/GH-M74a/GHA/2009 OX=1112631 GN=F PE=3 SV=1
MM1 pKa = 7.25 EE2 pKa = 6.66 AITNFMPYY10 pKa = 7.5 TWEE13 pKa = 4.35 FGGSLNKK20 pKa = 9.69 VEE22 pKa = 5.3 PEE24 pKa = 3.66 LDD26 pKa = 3.78 EE27 pKa = 4.7 NGSMIPKK34 pKa = 9.6 VRR36 pKa = 11.84 VLNPGYY42 pKa = 10.43 NDD44 pKa = 3.74 RR45 pKa = 11.84 KK46 pKa = 10.09 SSGYY50 pKa = 10.21 QYY52 pKa = 10.16 LICYY56 pKa = 8.97 GFVEE60 pKa = 5.43 DD61 pKa = 5.92 IRR63 pKa = 11.84 SEE65 pKa = 4.41 SNGDD69 pKa = 3.24 KK70 pKa = 10.71 KK71 pKa = 10.37 PTIRR75 pKa = 11.84 TTASFPLGVGRR86 pKa = 11.84 SDD88 pKa = 3.9 ASPEE92 pKa = 3.83 NLLDD96 pKa = 4.02 EE97 pKa = 4.45 VCKK100 pKa = 10.91 LSITVRR106 pKa = 11.84 RR107 pKa = 11.84 TSGAEE112 pKa = 3.56 EE113 pKa = 4.26 KK114 pKa = 10.25 IVYY117 pKa = 9.16 GVSGGILYY125 pKa = 10.24 LDD127 pKa = 3.59 PWKK130 pKa = 10.75 KK131 pKa = 9.98 VLKK134 pKa = 10.51 NGAIFTATKK143 pKa = 8.33 VCRR146 pKa = 11.84 NIEE149 pKa = 4.11 CVLVDD154 pKa = 2.93 KK155 pKa = 10.7 HH156 pKa = 5.78 QSLRR160 pKa = 11.84 IFFLSITKK168 pKa = 8.83 LTDD171 pKa = 2.71 RR172 pKa = 11.84 GYY174 pKa = 8.51 YY175 pKa = 8.3 TIPRR179 pKa = 11.84 RR180 pKa = 11.84 MLEE183 pKa = 3.61 FRR185 pKa = 11.84 YY186 pKa = 10.9 NNAIALNLLVTLAISTDD203 pKa = 3.48 TTKK206 pKa = 11.0 SGVRR210 pKa = 11.84 GIKK213 pKa = 10.43 NEE215 pKa = 4.22 DD216 pKa = 3.4 GFTLVTFMTHH226 pKa = 5.24 MGNFQRR232 pKa = 11.84 RR233 pKa = 11.84 AGKK236 pKa = 8.44 TYY238 pKa = 10.29 SLEE241 pKa = 4.02 YY242 pKa = 10.3 CSRR245 pKa = 11.84 KK246 pKa = 8.19 VDD248 pKa = 3.32 KK249 pKa = 10.64 MKK251 pKa = 10.56 MKK253 pKa = 10.57 FSLGAIGGLSFHH265 pKa = 6.03 VRR267 pKa = 11.84 IDD269 pKa = 3.57 GVISKK274 pKa = 10.54 RR275 pKa = 11.84 LFAEE279 pKa = 3.66 MGFRR283 pKa = 11.84 RR284 pKa = 11.84 NICYY288 pKa = 10.22 CLIDD292 pKa = 3.7 INPWINKK299 pKa = 6.94 LTWSNTCEE307 pKa = 3.95 IRR309 pKa = 11.84 NVAAVLQPSVPRR321 pKa = 11.84 DD322 pKa = 3.04 FMIYY326 pKa = 10.67 DD327 pKa = 4.21 DD328 pKa = 4.75 VFIDD332 pKa = 3.62 NTGKK336 pKa = 10.27 ILPSKK341 pKa = 10.14 HH342 pKa = 5.56 AA343 pKa = 4.26
Molecular weight: 38.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.667
IPC2_protein 8.551
IPC_protein 8.434
Toseland 8.975
ProMoST 8.946
Dawson 9.341
Bjellqvist 9.253
Wikipedia 9.502
Rodwell 9.516
Grimsley 9.355
Solomon 9.414
Lehninger 9.37
Nozaki 9.385
DTASelect 9.136
Thurlkill 9.238
EMBOSS 9.487
Sillero 9.414
Patrickios 4.609
IPC_peptide 9.399
IPC2_peptide 8.039
IPC2.peptide.svr19 7.724
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
5433
162
2250
776.1
87.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.822 ± 0.521
1.546 ± 0.305
5.393 ± 0.315
6.203 ± 0.442
3.258 ± 0.586
5.154 ± 0.446
1.859 ± 0.358
8.448 ± 0.674
6.35 ± 0.438
9.074 ± 0.648
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.558 ± 0.217
6.037 ± 0.438
4.859 ± 0.762
3.792 ± 0.298
5.503 ± 0.4
8.32 ± 0.308
6.534 ± 0.563
5.54 ± 0.142
0.92 ± 0.152
3.828 ± 0.583
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here