Marinobacterium stanieri

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Marinobacterium

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4329 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6XGG1|A0A1N6XGG1_9GAMM Uncharacterized protein OS=Marinobacterium stanieri OX=49186 GN=SAMN05421647_11414 PE=4 SV=1
MM1 pKa = 7.06STFFEE6 pKa = 4.74FKK8 pKa = 10.73DD9 pKa = 3.34NTVDD13 pKa = 3.84IFTSPSGNTFTEE25 pKa = 4.24VWSYY29 pKa = 11.47GGNDD33 pKa = 3.25QLTGNSGSDD42 pKa = 3.46YY43 pKa = 10.75FHH45 pKa = 7.42AGNGNDD51 pKa = 4.24YY52 pKa = 10.62LNGMAGDD59 pKa = 4.33DD60 pKa = 4.09VLDD63 pKa = 4.47GGAGHH68 pKa = 7.57DD69 pKa = 4.64DD70 pKa = 3.13IRR72 pKa = 11.84GGSGDD77 pKa = 4.45DD78 pKa = 4.22LLFGDD83 pKa = 5.09SGDD86 pKa = 3.62DD87 pKa = 3.34TLYY90 pKa = 11.3GGTGNDD96 pKa = 3.75LLRR99 pKa = 11.84GGEE102 pKa = 4.23GNDD105 pKa = 2.89EE106 pKa = 4.14YY107 pKa = 11.86ARR109 pKa = 11.84IQLRR113 pKa = 11.84SATLAYY119 pKa = 10.16AAA121 pKa = 4.94

Molecular weight: 12.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N6WQR9|A0A1N6WQR9_9GAMM ATP synthase subunit a OS=Marinobacterium stanieri OX=49186 GN=atpB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84QVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4329

0

4329

1420088

26

4828

328.0

36.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.77 ± 0.047

1.028 ± 0.013

5.777 ± 0.044

6.631 ± 0.036

3.637 ± 0.024

7.496 ± 0.051

2.275 ± 0.018

5.219 ± 0.029

3.649 ± 0.031

11.248 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.019

3.325 ± 0.027

4.514 ± 0.025

4.797 ± 0.037

5.938 ± 0.042

6.16 ± 0.029

5.073 ± 0.042

6.848 ± 0.029

1.344 ± 0.016

2.645 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski