Epulopiscium sp. Nele67-Bin005

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Epulopiscium; unclassified Epulopiscium

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1702 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S9CJ83|A0A1S9CJ83_9FIRM Cytosine-specific methyltransferase OS=Epulopiscium sp. Nele67-Bin005 OX=1764963 GN=ATN36_04610 PE=3 SV=1
MM1 pKa = 7.36NISTSMMNSYY11 pKa = 11.07YY12 pKa = 9.78STEE15 pKa = 4.1LLSTSTNNQTTQTEE29 pKa = 4.52QYY31 pKa = 10.76SDD33 pKa = 3.27VATQLGTASSTSSNTSTYY51 pKa = 10.82NNWEE55 pKa = 4.04NYY57 pKa = 8.58VSVVEE62 pKa = 4.16PDD64 pKa = 3.23GTRR67 pKa = 11.84DD68 pKa = 3.67YY69 pKa = 11.77YY70 pKa = 10.72EE71 pKa = 3.87VSYY74 pKa = 11.5VDD76 pKa = 3.58SDD78 pKa = 4.0GNNVTEE84 pKa = 5.39KK85 pKa = 10.65IKK87 pKa = 10.7LDD89 pKa = 3.65QINGHH94 pKa = 5.11GTSFAEE100 pKa = 4.27LAVLSSAGYY109 pKa = 9.18ISQDD113 pKa = 2.41IGTLLEE119 pKa = 4.07EE120 pKa = 4.51QFMDD124 pKa = 3.7EE125 pKa = 4.46NGEE128 pKa = 3.68IDD130 pKa = 3.71FNKK133 pKa = 9.97TVDD136 pKa = 3.34SLRR139 pKa = 11.84TVGDD143 pKa = 4.01TIRR146 pKa = 11.84DD147 pKa = 3.77NLHH150 pKa = 6.03GGDD153 pKa = 3.87YY154 pKa = 8.86ATAMKK159 pKa = 10.45FMGEE163 pKa = 4.1GKK165 pKa = 8.17TLNDD169 pKa = 4.04FFNDD173 pKa = 3.27TDD175 pKa = 3.82EE176 pKa = 4.71NDD178 pKa = 3.55PMTHH182 pKa = 7.03FLRR185 pKa = 11.84FMQINSTYY193 pKa = 10.81SDD195 pKa = 3.83DD196 pKa = 4.57SLLEE200 pKa = 3.87MLAA203 pKa = 3.84

Molecular weight: 22.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S9CMF8|A0A1S9CMF8_9FIRM UPF0145 protein ATN36_01730 OS=Epulopiscium sp. Nele67-Bin005 OX=1764963 GN=ATN36_01730 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.49KK9 pKa = 7.5RR10 pKa = 11.84QRR12 pKa = 11.84SRR14 pKa = 11.84EE15 pKa = 3.65HH16 pKa = 6.35GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.25SGRR28 pKa = 11.84AVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84TKK37 pKa = 10.37GRR39 pKa = 11.84KK40 pKa = 9.16KK41 pKa = 9.64LTAA44 pKa = 4.2

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1702

0

1702

560602

37

2932

329.4

37.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.121 ± 0.066

1.035 ± 0.024

5.416 ± 0.052

7.675 ± 0.074

4.272 ± 0.041

6.041 ± 0.06

1.733 ± 0.028

9.224 ± 0.068

6.82 ± 0.073

9.256 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.636 ± 0.032

5.923 ± 0.056

3.129 ± 0.044

3.788 ± 0.039

3.093 ± 0.041

6.207 ± 0.059

6.169 ± 0.091

6.699 ± 0.053

0.691 ± 0.017

4.07 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski