Actinomyces howellii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2533 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A448HH84|A0A448HH84_9ACTO Type-5 uracil-DNA glycosylase OS=Actinomyces howellii OX=52771 GN=NCTC11636_01532 PE=3 SV=1
MM1 pKa = 7.1TALNGLLEE9 pKa = 4.52AQGTVYY15 pKa = 9.23GTEE18 pKa = 3.99FLVDD22 pKa = 3.65LAGRR26 pKa = 11.84FEE28 pKa = 5.29DD29 pKa = 3.75NPYY32 pKa = 10.43DD33 pKa = 3.92ASSSGLEE40 pKa = 3.92PKK42 pKa = 10.72YY43 pKa = 10.62GGAYY47 pKa = 9.15EE48 pKa = 4.52GSSMDD53 pKa = 3.99PLYY56 pKa = 10.18GVCMAMSNNPEE67 pKa = 3.61AALTYY72 pKa = 7.16FTPDD76 pKa = 2.84GEE78 pKa = 4.53TGEE81 pKa = 4.54RR82 pKa = 11.84PGEE85 pKa = 3.85QSAEE89 pKa = 3.63RR90 pKa = 11.84WKK92 pKa = 11.15LLTEE96 pKa = 4.26RR97 pKa = 11.84TWDD100 pKa = 3.7PNVGLDD106 pKa = 3.6SLTGAVAAASAYY118 pKa = 10.6RR119 pKa = 11.84NTSDD123 pKa = 3.57STVPQADD130 pKa = 3.75ARR132 pKa = 11.84ATWLVGQAIPYY143 pKa = 7.82FAQDD147 pKa = 3.82DD148 pKa = 4.29APEE151 pKa = 4.09KK152 pKa = 9.84TEE154 pKa = 3.96TMKK157 pKa = 11.13EE158 pKa = 3.75NLAVVIGNSGEE169 pKa = 4.06EE170 pKa = 3.95FSAIASGQVIEE181 pKa = 5.89DD182 pKa = 3.67NVNDD186 pKa = 4.72LGLEE190 pKa = 4.63GYY192 pKa = 10.63VSTADD197 pKa = 3.85FEE199 pKa = 5.05SLVYY203 pKa = 10.53DD204 pKa = 4.76VIDD207 pKa = 3.84NEE209 pKa = 4.29NAAGTMAAGLGQYY222 pKa = 9.77HH223 pKa = 7.19HH224 pKa = 6.62GQIEE228 pKa = 4.64AIVAGSDD235 pKa = 3.5SPATGICDD243 pKa = 3.61QYY245 pKa = 11.52QGAAATMGYY254 pKa = 10.11LEE256 pKa = 4.78AVATRR261 pKa = 11.84RR262 pKa = 11.84AGDD265 pKa = 3.62DD266 pKa = 3.22EE267 pKa = 4.34AAKK270 pKa = 10.59QEE272 pKa = 4.19AKK274 pKa = 10.99GNVSTALSVFSTVLASGVGAVTGGTSWAVAGPLLYY309 pKa = 10.71GVGSTVAQPIVVDD322 pKa = 4.4EE323 pKa = 4.35VTEE326 pKa = 4.3DD327 pKa = 2.92WDD329 pKa = 4.2VPDD332 pKa = 4.11TVEE335 pKa = 5.83ADD337 pKa = 4.02DD338 pKa = 4.02IQNVLHH344 pKa = 6.11AQSYY348 pKa = 9.84VDD350 pKa = 3.33AARR353 pKa = 11.84YY354 pKa = 7.46GTLQSSTIAIGEE366 pKa = 4.38SIPTYY371 pKa = 10.71GGTEE375 pKa = 4.08PLCSQVDD382 pKa = 3.97GQVVVNAPDD391 pKa = 3.79PMTPDD396 pKa = 2.94AAAYY400 pKa = 8.9ILGWRR405 pKa = 11.84TAVTNSGSSDD415 pKa = 3.33QQMIDD420 pKa = 3.48LDD422 pKa = 4.05EE423 pKa = 4.42AVQTGWTKK431 pKa = 10.53GAEE434 pKa = 3.97QGKK437 pKa = 10.01EE438 pKa = 3.83GGEE441 pKa = 3.99

Molecular weight: 45.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S5EH41|A0A3S5EH41_9ACTO Peptidase M20 domain-containing protein 2 OS=Actinomyces howellii OX=52771 GN=abgB PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.4VHH17 pKa = 5.52GFRR20 pKa = 11.84KK21 pKa = 9.95RR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AVLAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.68GRR40 pKa = 11.84ARR42 pKa = 11.84LAAA45 pKa = 4.44

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2533

0

2533

897614

29

2012

354.4

37.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.723 ± 0.065

0.773 ± 0.015

5.857 ± 0.041

5.892 ± 0.048

2.302 ± 0.028

9.293 ± 0.045

1.983 ± 0.022

3.239 ± 0.034

1.441 ± 0.028

10.142 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.899 ± 0.02

1.596 ± 0.025

5.984 ± 0.046

2.854 ± 0.026

7.87 ± 0.054

5.982 ± 0.041

6.38 ± 0.046

9.391 ± 0.05

1.497 ± 0.019

1.901 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski