Solemya velesiana gill symbiont
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2304 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T2KSF8|A0A1T2KSF8_9GAMM Uncharacterized protein OS=Solemya velesiana gill symbiont OX=1918948 GN=BOW51_10330 PE=4 SV=1
MM1 pKa = 7.34 NIKK4 pKa = 9.76 VRR6 pKa = 11.84 EE7 pKa = 3.97 IMKK10 pKa = 9.76 KK11 pKa = 10.31 CGIVVCAALYY21 pKa = 10.13 IATPAFADD29 pKa = 3.49 DD30 pKa = 3.91 VEE32 pKa = 5.01 IFFNTSNVSGEE43 pKa = 4.6 DD44 pKa = 2.94 IGDD47 pKa = 3.93 PLVMFGLDD55 pKa = 3.35 YY56 pKa = 10.8 RR57 pKa = 11.84 PNVINANLCNAATLAEE73 pKa = 4.14 VQNCGWDD80 pKa = 3.71 DD81 pKa = 3.68 PTTTDD86 pKa = 5.32 DD87 pKa = 3.98 DD88 pKa = 4.28 DD89 pKa = 5.68 FYY91 pKa = 11.76 AAYY94 pKa = 10.98 YY95 pKa = 10.75 NVFTNEE101 pKa = 3.69 DD102 pKa = 3.54 VADD105 pKa = 4.51 GSLSFLEE112 pKa = 4.08 VLRR115 pKa = 11.84 ASLRR119 pKa = 11.84 YY120 pKa = 9.72 VLGEE124 pKa = 4.07 LDD126 pKa = 3.82 DD127 pKa = 3.97 VRR129 pKa = 11.84 IGLMLNHH136 pKa = 6.3 AQINNCEE143 pKa = 4.26 GASGTAGCSNGGYY156 pKa = 9.52 IAMGLLPIDD165 pKa = 4.4 AVDD168 pKa = 5.09 NSGGWYY174 pKa = 6.55 TTWLDD179 pKa = 3.28 NDD181 pKa = 3.79 GDD183 pKa = 4.35 GVHH186 pKa = 7.15 DD187 pKa = 4.42 VDD189 pKa = 6.02 AGDD192 pKa = 5.15 DD193 pKa = 3.78 PSTGLNILDD202 pKa = 4.31 GKK204 pKa = 10.01 LASILDD210 pKa = 4.09 LGTGAGAHH218 pKa = 5.84 SNQGKK223 pKa = 8.86 EE224 pKa = 4.4 LYY226 pKa = 10.32 FEE228 pKa = 4.87 LYY230 pKa = 9.98 RR231 pKa = 11.84 YY232 pKa = 9.85 LRR234 pKa = 11.84 GWDD237 pKa = 3.11 IYY239 pKa = 10.69 NGHH242 pKa = 6.46 VGYY245 pKa = 11.0 ADD247 pKa = 3.48 YY248 pKa = 11.14 DD249 pKa = 3.95 DD250 pKa = 6.02 RR251 pKa = 11.84 CDD253 pKa = 4.71 EE254 pKa = 5.55 DD255 pKa = 5.56 NLDD258 pKa = 4.53 DD259 pKa = 5.12 DD260 pKa = 4.78 TMPSCFDD267 pKa = 3.1 RR268 pKa = 11.84 RR269 pKa = 11.84 GNPEE273 pKa = 4.95 DD274 pKa = 5.59 DD275 pKa = 4.52 DD276 pKa = 5.5 DD277 pKa = 4.77 YY278 pKa = 11.84 PAIAWDD284 pKa = 3.58 ATTVGDD290 pKa = 3.81 VGIGATAPDD299 pKa = 4.27 YY300 pKa = 10.73 PVEE303 pKa = 4.11 EE304 pKa = 4.77 AGNTEE309 pKa = 4.48 YY310 pKa = 11.2 YY311 pKa = 10.86 SPFEE315 pKa = 4.42 DD316 pKa = 4.19 QSLICSGVYY325 pKa = 10.31 AINFMFGVSNQDD337 pKa = 2.84 NDD339 pKa = 3.6 SDD341 pKa = 5.32 NEE343 pKa = 4.1 IEE345 pKa = 5.85 DD346 pKa = 4.72 DD347 pKa = 4.33 DD348 pKa = 5.45 AGQISAEE355 pKa = 4.07 SAGMVEE361 pKa = 5.54 LDD363 pKa = 3.59 GSDD366 pKa = 4.57 PIDD369 pKa = 3.15 ISGNRR374 pKa = 11.84 NEE376 pKa = 4.65 FGTLIEE382 pKa = 4.03 WLYY385 pKa = 10.44 EE386 pKa = 4.2 GSDD389 pKa = 3.54 GSPRR393 pKa = 11.84 DD394 pKa = 3.59 LSDD397 pKa = 3.22 LAGVQNVTSYY407 pKa = 11.42 FMYY410 pKa = 10.24 KK411 pKa = 10.69 GNVQNTMNGYY421 pKa = 9.51 AVSGGSGQAYY431 pKa = 8.74 EE432 pKa = 4.61 VLDD435 pKa = 4.31 DD436 pKa = 4.41 PKK438 pKa = 11.85 AMVDD442 pKa = 3.83 TITNIFKK449 pKa = 10.63 EE450 pKa = 4.17 ILRR453 pKa = 11.84 QNTTFEE459 pKa = 4.31 APAVTVNSYY468 pKa = 10.73 NRR470 pKa = 11.84 LTHH473 pKa = 6.79 RR474 pKa = 11.84 DD475 pKa = 3.57 EE476 pKa = 5.45 LFFALFSQEE485 pKa = 4.06 EE486 pKa = 4.22 HH487 pKa = 6.26 QNWPGNLKK495 pKa = 10.17 KK496 pKa = 10.91 YY497 pKa = 10.52 KK498 pKa = 10.16 LGEE501 pKa = 3.91 ITTEE505 pKa = 3.73 VCTDD509 pKa = 3.72 LNGDD513 pKa = 4.34 GDD515 pKa = 4.07 TTDD518 pKa = 3.69 PGEE521 pKa = 4.53 CVTEE525 pKa = 3.97 PVQRR529 pKa = 11.84 LVDD532 pKa = 3.73 QNDD535 pKa = 3.41 NEE537 pKa = 4.84 AVNQSDD543 pKa = 4.33 GVFKK547 pKa = 10.58 ATTCSFWSNCSIDD560 pKa = 3.72 RR561 pKa = 11.84 DD562 pKa = 3.79 NDD564 pKa = 3.25 GTADD568 pKa = 3.69 ADD570 pKa = 3.84 GDD572 pKa = 3.93 IVEE575 pKa = 4.49 WGGAAEE581 pKa = 5.05 EE582 pKa = 4.32 ITLSRR587 pKa = 11.84 TLYY590 pKa = 10.2 TYY592 pKa = 10.6 IGTDD596 pKa = 3.0 RR597 pKa = 11.84 YY598 pKa = 10.45 AVHH601 pKa = 7.31 EE602 pKa = 4.45 DD603 pKa = 3.29 TSQITHH609 pKa = 5.93 EE610 pKa = 4.4 MLNVSLDD617 pKa = 3.67 EE618 pKa = 4.46 TSVHH622 pKa = 6.82 AGDD625 pKa = 5.88 DD626 pKa = 3.56 ICDD629 pKa = 3.4 VDD631 pKa = 4.45 GDD633 pKa = 3.97 SDD635 pKa = 4.62 GDD637 pKa = 4.06 FEE639 pKa = 5.01 CVDD642 pKa = 3.47 MDD644 pKa = 4.56 GDD646 pKa = 3.99 GDD648 pKa = 4.72 ADD650 pKa = 4.03 STDD653 pKa = 3.3 HH654 pKa = 7.73 DD655 pKa = 4.85 LLRR658 pKa = 11.84 EE659 pKa = 4.22 DD660 pKa = 4.84 LLQIARR666 pKa = 11.84 SYY668 pKa = 11.3 DD669 pKa = 3.53 PEE671 pKa = 4.33 TGEE674 pKa = 4.26 VLQNMGDD681 pKa = 4.24 PIHH684 pKa = 6.84 ARR686 pKa = 11.84 PAVIEE691 pKa = 3.92 YY692 pKa = 10.43 DD693 pKa = 3.52 ADD695 pKa = 3.85 LSNQGACPDD704 pKa = 3.94 LRR706 pKa = 11.84 IAMTTNEE713 pKa = 4.4 GQFHH717 pKa = 7.2 LIDD720 pKa = 4.26 AGGDD724 pKa = 3.63 SGCTDD729 pKa = 2.95 NDD731 pKa = 3.82 ANVGGTEE738 pKa = 4.19 VVSFMPEE745 pKa = 3.4 EE746 pKa = 4.49 LLPQLKK752 pKa = 9.35 QVNGPITVEE761 pKa = 4.02 GTGQTRR767 pKa = 11.84 FNTYY771 pKa = 10.49 GLDD774 pKa = 3.47 GSPVVWRR781 pKa = 11.84 YY782 pKa = 9.93 DD783 pKa = 3.12 ADD785 pKa = 4.68 AGDD788 pKa = 3.62 GTARR792 pKa = 11.84 DD793 pKa = 3.71 GVINYY798 pKa = 8.94 GADD801 pKa = 3.33 SDD803 pKa = 4.09 EE804 pKa = 4.59 DD805 pKa = 3.74 FVYY808 pKa = 10.84 LFLTQRR814 pKa = 11.84 RR815 pKa = 11.84 GGKK818 pKa = 9.85 GIWALDD824 pKa = 3.34 ITNPTSPEE832 pKa = 3.99 LMWKK836 pKa = 9.39 VWDD839 pKa = 4.14 GDD841 pKa = 3.88 LTSVNGTSAADD852 pKa = 3.57 VYY854 pKa = 11.57 SEE856 pKa = 5.77 LGQTWSTPKK865 pKa = 9.25 LTKK868 pKa = 10.35 IKK870 pKa = 10.65 DD871 pKa = 3.41 GEE873 pKa = 4.08 YY874 pKa = 10.77 DD875 pKa = 3.09 RR876 pKa = 11.84 GVLVFGGGYY885 pKa = 10.73 DD886 pKa = 3.71 PDD888 pKa = 3.66 QDD890 pKa = 3.8 DD891 pKa = 5.14 AYY893 pKa = 11.31 SFSDD897 pKa = 3.36 TQGRR901 pKa = 11.84 AIYY904 pKa = 10.53 VVDD907 pKa = 4.95 AYY909 pKa = 9.81 TGEE912 pKa = 4.28 PLWTVSGEE920 pKa = 4.25 AASTTPTQPSGGASGTMLIPEE941 pKa = 4.72 MDD943 pKa = 3.33 NSIPAEE949 pKa = 4.06 VQPADD954 pKa = 3.9 IDD956 pKa = 3.81 SDD958 pKa = 4.41 GYY960 pKa = 11.04 LDD962 pKa = 4.94 RR963 pKa = 11.84 IYY965 pKa = 11.18 AVDD968 pKa = 3.33 VVGRR972 pKa = 11.84 VFRR975 pKa = 11.84 IDD977 pKa = 5.0 FDD979 pKa = 3.67 ILPNGDD985 pKa = 3.57 STSTQMYY992 pKa = 8.52 GGGMVAALNDD1002 pKa = 3.78 SSCDD1006 pKa = 3.33 EE1007 pKa = 4.24 EE1008 pKa = 6.17 APTDD1012 pKa = 4.01 GVADD1016 pKa = 3.61 ACHH1019 pKa = 6.02 RR1020 pKa = 11.84 RR1021 pKa = 11.84 FYY1023 pKa = 10.94 NKK1025 pKa = 9.32 PDD1027 pKa = 3.26 VAVMVGYY1034 pKa = 8.61 PAAPYY1039 pKa = 9.18 VQVAVGSGYY1048 pKa = 10.22 RR1049 pKa = 11.84 AWPASVSGIQDD1060 pKa = 3.31 RR1061 pKa = 11.84 FYY1063 pKa = 11.69 VLFDD1067 pKa = 3.95 EE1068 pKa = 5.37 NVIDD1072 pKa = 4.33 PLLSSVYY1079 pKa = 9.13 GTGAGEE1085 pKa = 4.1 QYY1087 pKa = 10.81 HH1088 pKa = 5.12 WTEE1091 pKa = 5.01 DD1092 pKa = 3.24 SDD1094 pKa = 4.56 LANLTDD1100 pKa = 3.4 IPGDD1104 pKa = 3.41 VSDD1107 pKa = 4.3 YY1108 pKa = 11.51 SGAQTAAEE1116 pKa = 3.88 TALGNKK1122 pKa = 8.96 HH1123 pKa = 5.02 GWYY1126 pKa = 10.35 FNLEE1130 pKa = 4.14 LTTDD1134 pKa = 3.15 AGGTVTSHH1142 pKa = 6.36 EE1143 pKa = 4.54 KK1144 pKa = 10.62 VLSDD1148 pKa = 3.17 SVTFNGLILFTTYY1161 pKa = 9.86 IRR1163 pKa = 11.84 SSEE1166 pKa = 4.17 DD1167 pKa = 3.2 EE1168 pKa = 4.54 ANVCEE1173 pKa = 4.29 PSLGSGRR1180 pKa = 11.84 FYY1182 pKa = 11.31 AVNAFNGLPVGDD1194 pKa = 4.02 INQGVFFTEE1203 pKa = 3.84 DD1204 pKa = 2.94 TARR1207 pKa = 11.84 SDD1209 pKa = 3.27 FSEE1212 pKa = 4.06 ADD1214 pKa = 3.04 RR1215 pKa = 11.84 YY1216 pKa = 10.21 MNLGRR1221 pKa = 11.84 KK1222 pKa = 8.66 GIPTTPQIILTDD1234 pKa = 3.56 EE1235 pKa = 4.53 GPRR1238 pKa = 11.84 VIVTTEE1244 pKa = 4.69 LIPEE1248 pKa = 4.43 GLLDD1252 pKa = 4.61 SDD1254 pKa = 5.07 LFSKK1258 pKa = 10.21 KK1259 pKa = 7.04 WWLDD1263 pKa = 3.16 AKK1265 pKa = 10.96
Molecular weight: 137.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.732
IPC_protein 3.795
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.745
Nozaki 3.884
DTASelect 4.164
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.91
Patrickios 1.074
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A1T2KXJ6|A0A1T2KXJ6_9GAMM Phage-tail_3 domain-containing protein OS=Solemya velesiana gill symbiont OX=1918948 GN=BOW51_02575 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.08 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.92 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 NGRR28 pKa = 11.84 KK29 pKa = 9.4 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.1 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTPP44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2304
0
2304
693604
31
15124
301.0
33.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.078 ± 0.056
0.995 ± 0.027
5.965 ± 0.113
7.245 ± 0.056
3.664 ± 0.036
7.841 ± 0.084
2.266 ± 0.034
5.786 ± 0.039
4.354 ± 0.07
10.475 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.733 ± 0.046
3.323 ± 0.044
4.467 ± 0.044
3.858 ± 0.043
6.088 ± 0.097
5.866 ± 0.042
5.008 ± 0.092
7.148 ± 0.048
1.206 ± 0.021
2.634 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here