Human rotavirus A

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222NY09|A0A222NY09_9REOV RNA-directed RNA polymerase (Fragment) OS=Human rotavirus A OX=10941 GN=VP1 PE=4 SV=1
MM1 pKa = 7.26YY2 pKa = 10.32GIEE5 pKa = 4.31YY6 pKa = 6.88TTLLIFLISIILLNYY21 pKa = 8.54ILKK24 pKa = 10.16SVTRR28 pKa = 11.84IMDD31 pKa = 3.48YY32 pKa = 10.32IIYY35 pKa = 9.99RR36 pKa = 11.84FLLIFVALFTLTKK49 pKa = 9.29AQNYY53 pKa = 8.48GLNIPITGSMDD64 pKa = 3.33TVYY67 pKa = 11.2SNSTRR72 pKa = 11.84EE73 pKa = 4.1EE74 pKa = 4.04VFLTSTLCLYY84 pKa = 10.82YY85 pKa = 8.95PTEE88 pKa = 4.43ASTQISDD95 pKa = 4.87GEE97 pKa = 4.42WKK99 pKa = 10.54DD100 pKa = 3.71SLSQMLLTKK109 pKa = 9.97GWPTGSVYY117 pKa = 10.45FKK119 pKa = 9.95EE120 pKa = 4.2YY121 pKa = 11.11SNIVDD126 pKa = 4.4FSVDD130 pKa = 3.21PQLYY134 pKa = 9.83CDD136 pKa = 3.92YY137 pKa = 11.01NLVLMKK143 pKa = 10.38YY144 pKa = 9.67DD145 pKa = 3.61QNLEE149 pKa = 3.95LDD151 pKa = 3.67MSEE154 pKa = 4.77LADD157 pKa = 5.13LILNEE162 pKa = 4.24WLCNPMDD169 pKa = 3.29ITLYY173 pKa = 10.57YY174 pKa = 8.41YY175 pKa = 10.05QQSGEE180 pKa = 4.25SNKK183 pKa = 8.88WISMGSSCTVKK194 pKa = 10.28VCPLNTQTLGIGCQTTNVDD213 pKa = 3.15SFEE216 pKa = 4.26TVAEE220 pKa = 4.06NEE222 pKa = 4.25KK223 pKa = 10.37LAIMDD228 pKa = 3.58VVDD231 pKa = 6.03GINHH235 pKa = 7.52KK236 pKa = 10.62INLTTTTCTIRR247 pKa = 11.84NCKK250 pKa = 9.83KK251 pKa = 10.18LGPRR255 pKa = 11.84EE256 pKa = 4.01NVAVIQVGGANILDD270 pKa = 3.6ITADD274 pKa = 3.64PTTNPQIEE282 pKa = 4.06RR283 pKa = 11.84MMRR286 pKa = 11.84VNWKK290 pKa = 9.46RR291 pKa = 11.84WWQVFYY297 pKa = 10.68TIVDD301 pKa = 3.85YY302 pKa = 11.03INQIVQVMSKK312 pKa = 10.17RR313 pKa = 11.84SRR315 pKa = 11.84SLNSAAFYY323 pKa = 11.0YY324 pKa = 10.27RR325 pKa = 11.84VV326 pKa = 3.19

Molecular weight: 37.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5KR58|Q5KR58_9REOV Non-structural glycoprotein 4 OS=Human rotavirus A OX=10941 GN=NSP4 PE=3 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.64LACFCYY9 pKa = 9.75PHH11 pKa = 7.6LEE13 pKa = 3.86NDD15 pKa = 3.51SYY17 pKa = 12.02KK18 pKa = 10.72FIPFNNLAIKK28 pKa = 10.63CMLTAKK34 pKa = 9.91VDD36 pKa = 3.91KK37 pKa = 10.63KK38 pKa = 11.12DD39 pKa = 3.04QDD41 pKa = 3.13KK42 pKa = 10.47FYY44 pKa = 11.27NSIVYY49 pKa = 9.72GIAPPPQFRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 6.98NTSDD65 pKa = 2.85NSRR68 pKa = 11.84GMNYY72 pKa = 7.27EE73 pKa = 3.71TIMFNKK79 pKa = 9.36VAILICEE86 pKa = 4.24ALNSIKK92 pKa = 9.46VTQSEE97 pKa = 4.68VASVLSRR104 pKa = 11.84VVSVRR109 pKa = 11.84HH110 pKa = 5.93LEE112 pKa = 3.89NLVLRR117 pKa = 11.84KK118 pKa = 9.59EE119 pKa = 4.02NHH121 pKa = 5.63QDD123 pKa = 3.26VLFHH127 pKa = 7.13SKK129 pKa = 10.35EE130 pKa = 3.99LLLKK134 pKa = 10.55SVLIAIGQSKK144 pKa = 10.17EE145 pKa = 3.74IEE147 pKa = 4.2TTATAEE153 pKa = 3.87GGEE156 pKa = 4.08IVFQNAAFTMWKK168 pKa = 8.49LTYY171 pKa = 9.82LDD173 pKa = 4.31HH174 pKa = 8.04KK175 pKa = 10.88LMPILDD181 pKa = 3.78QNFIEE186 pKa = 5.0YY187 pKa = 10.32KK188 pKa = 8.98ITLNEE193 pKa = 4.63DD194 pKa = 3.16KK195 pKa = 10.28PISDD199 pKa = 3.74VCVKK203 pKa = 10.44EE204 pKa = 4.33IVAEE208 pKa = 4.51LRR210 pKa = 11.84WQYY213 pKa = 11.68NRR215 pKa = 11.84FAVITHH221 pKa = 6.3GKK223 pKa = 7.43GHH225 pKa = 5.73YY226 pKa = 9.48RR227 pKa = 11.84VVKK230 pKa = 8.49YY231 pKa = 10.96SSVANHH237 pKa = 7.18ADD239 pKa = 3.4RR240 pKa = 11.84VFATYY245 pKa = 10.85KK246 pKa = 10.88NNAKK250 pKa = 10.33SGNTTDD256 pKa = 6.24FNLLDD261 pKa = 3.63QRR263 pKa = 11.84IILANWYY270 pKa = 10.38AFTSSMKK277 pKa = 10.12QGNTIDD283 pKa = 3.22VCRR286 pKa = 11.84RR287 pKa = 11.84LLFPKK292 pKa = 9.92MKK294 pKa = 10.14QEE296 pKa = 4.0KK297 pKa = 10.31NPFKK301 pKa = 10.91GLSTDD306 pKa = 2.99RR307 pKa = 11.84KK308 pKa = 8.05MDD310 pKa = 3.68EE311 pKa = 4.12VSHH314 pKa = 6.23VGG316 pKa = 3.18

Molecular weight: 36.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

3097

175

479

281.5

32.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.488 ± 0.64

1.744 ± 0.468

6.426 ± 0.495

5.747 ± 0.652

3.972 ± 0.454

3.358 ± 0.343

1.614 ± 0.355

7.846 ± 0.352

7.039 ± 1.063

8.783 ± 0.478

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.841 ± 0.317

7.427 ± 0.403

3.294 ± 0.452

4.133 ± 0.421

4.747 ± 0.316

7.653 ± 1.013

6.522 ± 0.625

6.103 ± 0.326

1.356 ± 0.211

4.908 ± 0.564
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski