Marinomonas phage CPP1m

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Colwellvirinae; Murciavirus; Marinomonas virus CPP1m

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W5S8V1|A0A1W5S8V1_9CAUD Uncharacterized protein OS=Marinomonas phage CPP1m OX=1965370 PE=4 SV=1
MM1 pKa = 7.86ADD3 pKa = 3.05SSLSYY8 pKa = 10.64TEE10 pKa = 3.94YY11 pKa = 10.63TGDD14 pKa = 3.76GATKK18 pKa = 10.54DD19 pKa = 3.61FALSVSGEE27 pKa = 4.19DD28 pKa = 2.37IGYY31 pKa = 9.51IRR33 pKa = 11.84TGDD36 pKa = 2.78IHH38 pKa = 7.81GYY40 pKa = 7.98VDD42 pKa = 3.77EE43 pKa = 4.99VEE45 pKa = 4.36VSVSIDD51 pKa = 3.17VNTPHH56 pKa = 7.22IVTFEE61 pKa = 4.04SAPALGSSILISRR74 pKa = 11.84EE75 pKa = 3.84MEE77 pKa = 4.21DD78 pKa = 3.36EE79 pKa = 4.15SPYY82 pKa = 9.73VTFQRR87 pKa = 11.84GSDD90 pKa = 3.62FSNTNVNNSLRR101 pKa = 11.84HH102 pKa = 5.41LLYY105 pKa = 10.87LIQEE109 pKa = 4.16WRR111 pKa = 11.84NGFLPSYY118 pKa = 10.41HH119 pKa = 6.36SLKK122 pKa = 10.55SDD124 pKa = 4.0LDD126 pKa = 4.22AGDD129 pKa = 4.8NIITNVKK136 pKa = 10.14SPEE139 pKa = 4.25LGSDD143 pKa = 3.35AVNLEE148 pKa = 4.09TLEE151 pKa = 4.49SYY153 pKa = 10.89SADD156 pKa = 2.95LTAALEE162 pKa = 4.07AAIAAQGAVDD172 pKa = 4.33ASQDD176 pKa = 3.44SVTQSISDD184 pKa = 3.26RR185 pKa = 11.84VLTLEE190 pKa = 4.64GGSEE194 pKa = 4.16VTIGTSYY201 pKa = 10.59RR202 pKa = 11.84YY203 pKa = 9.09PIKK206 pKa = 10.17RR207 pKa = 11.84TAQEE211 pKa = 4.03GQTTFDD217 pKa = 3.89LVLDD221 pKa = 3.75VDD223 pKa = 4.28TNSIVSINGATQVPLEE239 pKa = 4.25AYY241 pKa = 8.03EE242 pKa = 4.34INGNTLTFAEE252 pKa = 4.75GLEE255 pKa = 4.24EE256 pKa = 5.37GDD258 pKa = 3.78VVLVLAGFDD267 pKa = 3.69YY268 pKa = 11.39SPVEE272 pKa = 4.13VVGEE276 pKa = 4.1GDD278 pKa = 3.14WVYY281 pKa = 11.27SAIGGEE287 pKa = 4.38VVLDD291 pKa = 3.44TTTAFDD297 pKa = 5.14SILLFINGVAQTPNYY312 pKa = 9.82AFSASGTVITLAEE325 pKa = 4.11ALEE328 pKa = 4.6AGDD331 pKa = 4.32LVYY334 pKa = 10.71AILKK338 pKa = 7.39NTT340 pKa = 3.71

Molecular weight: 36.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W5SAB5|A0A1W5SAB5_9CAUD Capsid and scaffold protein OS=Marinomonas phage CPP1m OX=1965370 PE=4 SV=1
MM1 pKa = 7.51TDD3 pKa = 3.88KK4 pKa = 11.07LIQAISSAAACNKK17 pKa = 9.79NNPNNLPNIRR27 pKa = 11.84KK28 pKa = 8.81EE29 pKa = 3.91LIKK32 pKa = 10.07RR33 pKa = 11.84QKK35 pKa = 10.16GICPISGINLKK46 pKa = 10.38AVAASNVVVDD56 pKa = 4.7HH57 pKa = 6.64DD58 pKa = 4.64HH59 pKa = 5.53EE60 pKa = 4.65TGIIRR65 pKa = 11.84AALPRR70 pKa = 11.84ALNGLEE76 pKa = 3.89GKK78 pKa = 9.71LVNLCIRR85 pKa = 11.84WGRR88 pKa = 11.84CKK90 pKa = 10.39SKK92 pKa = 10.84RR93 pKa = 11.84DD94 pKa = 3.45IIQLLRR100 pKa = 11.84SMADD104 pKa = 3.36YY105 pKa = 10.88LEE107 pKa = 4.64HH108 pKa = 7.06HH109 pKa = 7.09LTPQTEE115 pKa = 4.88WIHH118 pKa = 5.42PTHH121 pKa = 6.66LTPLQKK127 pKa = 10.03RR128 pKa = 11.84AKK130 pKa = 9.87ANEE133 pKa = 3.73AARR136 pKa = 11.84KK137 pKa = 9.06RR138 pKa = 11.84RR139 pKa = 11.84AAKK142 pKa = 10.22KK143 pKa = 9.82EE144 pKa = 3.81RR145 pKa = 3.57

Molecular weight: 16.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

13937

39

1401

278.7

31.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.785 ± 0.343

0.969 ± 0.154

6.429 ± 0.211

8.007 ± 0.388

3.588 ± 0.142

6.845 ± 0.312

1.765 ± 0.177

5.597 ± 0.204

6.91 ± 0.369

8.194 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.233

4.807 ± 0.236

3.214 ± 0.146

3.451 ± 0.253

4.843 ± 0.217

7.555 ± 0.398

5.776 ± 0.319

6.465 ± 0.249

1.177 ± 0.132

3.853 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski