Amino acid dipepetide frequency for Escherichia phage P13803

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.219AlaAla: 9.219 ± 0.758
1.072AlaCys: 1.072 ± 0.315
5.414AlaAsp: 5.414 ± 0.617
8.361AlaGlu: 8.361 ± 0.696
3.806AlaPhe: 3.806 ± 0.552
8.576AlaGly: 8.576 ± 1.454
1.822AlaHis: 1.822 ± 0.303
4.395AlaIle: 4.395 ± 0.63
5.199AlaLys: 5.199 ± 0.497
6.7AlaLeu: 6.7 ± 0.53
3.055AlaMet: 3.055 ± 0.418
2.626AlaAsn: 2.626 ± 0.372
3.591AlaPro: 3.591 ± 0.463
4.985AlaGln: 4.985 ± 0.711
5.628AlaArg: 5.628 ± 0.626
5.896AlaSer: 5.896 ± 0.502
5.467AlaThr: 5.467 ± 0.608
6.378AlaVal: 6.378 ± 0.634
1.608AlaTrp: 1.608 ± 0.302
2.626AlaTyr: 2.626 ± 0.312
0.0AlaXaa: 0.0 ± 0.0
Cys
1.179CysAla: 1.179 ± 0.271
0.214CysCys: 0.214 ± 0.115
0.697CysAsp: 0.697 ± 0.25
0.59CysGlu: 0.59 ± 0.196
0.643CysPhe: 0.643 ± 0.201
1.072CysGly: 1.072 ± 0.356
0.214CysHis: 0.214 ± 0.117
0.482CysIle: 0.482 ± 0.173
0.59CysLys: 0.59 ± 0.198
1.018CysLeu: 1.018 ± 0.293
0.322CysMet: 0.322 ± 0.135
0.429CysAsn: 0.429 ± 0.188
0.643CysPro: 0.643 ± 0.228
0.375CysGln: 0.375 ± 0.146
0.965CysArg: 0.965 ± 0.338
1.018CysSer: 1.018 ± 0.288
0.429CysThr: 0.429 ± 0.136
0.911CysVal: 0.911 ± 0.274
0.161CysTrp: 0.161 ± 0.104
0.375CysTyr: 0.375 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
6.11AspAla: 6.11 ± 0.668
0.536AspCys: 0.536 ± 0.192
3.538AspAsp: 3.538 ± 0.393
3.966AspGlu: 3.966 ± 0.436
1.983AspPhe: 1.983 ± 0.367
3.859AspGly: 3.859 ± 0.528
0.858AspHis: 0.858 ± 0.29
3.109AspIle: 3.109 ± 0.386
3.966AspLys: 3.966 ± 0.393
4.288AspLeu: 4.288 ± 0.518
1.608AspMet: 1.608 ± 0.305
2.412AspAsn: 2.412 ± 0.365
2.358AspPro: 2.358 ± 0.455
1.501AspGln: 1.501 ± 0.325
3.538AspArg: 3.538 ± 0.458
2.948AspSer: 2.948 ± 0.388
2.787AspThr: 2.787 ± 0.393
4.449AspVal: 4.449 ± 0.445
0.75AspTrp: 0.75 ± 0.265
1.608AspTyr: 1.608 ± 0.265
0.0AspXaa: 0.0 ± 0.0
Glu
6.539GluAla: 6.539 ± 0.644
0.965GluCys: 0.965 ± 0.266
2.519GluAsp: 2.519 ± 0.326
4.502GluGlu: 4.502 ± 0.488
2.626GluPhe: 2.626 ± 0.441
3.806GluGly: 3.806 ± 0.537
1.447GluHis: 1.447 ± 0.257
3.806GluIle: 3.806 ± 0.472
4.61GluLys: 4.61 ± 0.64
6.003GluLeu: 6.003 ± 0.603
2.251GluMet: 2.251 ± 0.385
2.626GluAsn: 2.626 ± 0.435
1.608GluPro: 1.608 ± 0.343
3.484GluGln: 3.484 ± 0.547
5.092GluArg: 5.092 ± 0.451
3.323GluSer: 3.323 ± 0.381
3.323GluThr: 3.323 ± 0.655
4.395GluVal: 4.395 ± 0.635
0.75GluTrp: 0.75 ± 0.177
2.251GluTyr: 2.251 ± 0.287
0.0GluXaa: 0.0 ± 0.0
Phe
3.002PheAla: 3.002 ± 0.445
0.75PheCys: 0.75 ± 0.215
2.305PheAsp: 2.305 ± 0.354
1.501PheGlu: 1.501 ± 0.337
1.018PhePhe: 1.018 ± 0.244
2.412PheGly: 2.412 ± 0.319
0.643PheHis: 0.643 ± 0.154
1.876PheIle: 1.876 ± 0.33
1.876PheLys: 1.876 ± 0.287
2.251PheLeu: 2.251 ± 0.288
1.394PheMet: 1.394 ± 0.279
1.93PheAsn: 1.93 ± 0.349
1.34PhePro: 1.34 ± 0.308
0.804PheGln: 0.804 ± 0.222
2.841PheArg: 2.841 ± 0.475
3.591PheSer: 3.591 ± 0.552
2.144PheThr: 2.144 ± 0.282
2.948PheVal: 2.948 ± 0.406
0.536PheTrp: 0.536 ± 0.189
0.911PheTyr: 0.911 ± 0.182
0.0PheXaa: 0.0 ± 0.0
Gly
6.646GlyAla: 6.646 ± 1.047
0.59GlyCys: 0.59 ± 0.222
4.77GlyAsp: 4.77 ± 0.94
5.842GlyGlu: 5.842 ± 1.171
3.055GlyPhe: 3.055 ± 0.38
5.36GlyGly: 5.36 ± 0.72
0.965GlyHis: 0.965 ± 0.212
4.127GlyIle: 4.127 ± 0.602
5.199GlyLys: 5.199 ± 0.808
5.038GlyLeu: 5.038 ± 0.411
2.09GlyMet: 2.09 ± 0.365
2.841GlyAsn: 2.841 ± 0.378
4.127GlyPro: 4.127 ± 2.606
3.109GlyGln: 3.109 ± 0.469
4.449GlyArg: 4.449 ± 0.486
4.234GlySer: 4.234 ± 0.539
3.752GlyThr: 3.752 ± 0.454
5.199GlyVal: 5.199 ± 0.552
0.911GlyTrp: 0.911 ± 0.186
2.519GlyTyr: 2.519 ± 0.328
0.0GlyXaa: 0.0 ± 0.0
His
1.876HisAla: 1.876 ± 0.198
0.107HisCys: 0.107 ± 0.067
0.697HisAsp: 0.697 ± 0.173
0.911HisGlu: 0.911 ± 0.248
0.697HisPhe: 0.697 ± 0.224
1.608HisGly: 1.608 ± 0.335
0.375HisHis: 0.375 ± 0.137
0.804HisIle: 0.804 ± 0.22
0.804HisLys: 0.804 ± 0.227
1.822HisLeu: 1.822 ± 0.425
0.536HisMet: 0.536 ± 0.148
0.697HisAsn: 0.697 ± 0.199
0.911HisPro: 0.911 ± 0.249
0.697HisGln: 0.697 ± 0.225
0.965HisArg: 0.965 ± 0.225
1.286HisSer: 1.286 ± 0.221
0.858HisThr: 0.858 ± 0.199
0.697HisVal: 0.697 ± 0.188
0.322HisTrp: 0.322 ± 0.165
0.697HisTyr: 0.697 ± 0.171
0.0HisXaa: 0.0 ± 0.0
Ile
4.931IleAla: 4.931 ± 0.457
0.965IleCys: 0.965 ± 0.283
3.591IleAsp: 3.591 ± 0.436
2.894IleGlu: 2.894 ± 0.45
1.233IlePhe: 1.233 ± 0.29
2.412IleGly: 2.412 ± 0.418
0.911IleHis: 0.911 ± 0.189
2.305IleIle: 2.305 ± 0.397
2.734IleLys: 2.734 ± 0.356
3.216IleLeu: 3.216 ± 0.493
1.126IleMet: 1.126 ± 0.248
3.216IleAsn: 3.216 ± 0.485
2.358IlePro: 2.358 ± 0.3
2.037IleGln: 2.037 ± 0.28
4.77IleArg: 4.77 ± 0.471
4.02IleSer: 4.02 ± 0.671
3.216IleThr: 3.216 ± 0.427
2.144IleVal: 2.144 ± 0.421
0.268IleTrp: 0.268 ± 0.139
1.072IleTyr: 1.072 ± 0.233
0.0IleXaa: 0.0 ± 0.0
Lys
6.325LysAla: 6.325 ± 0.504
0.697LysCys: 0.697 ± 0.254
2.948LysAsp: 2.948 ± 0.43
3.484LysGlu: 3.484 ± 0.45
1.447LysPhe: 1.447 ± 0.291
6.003LysGly: 6.003 ± 1.412
1.018LysHis: 1.018 ± 0.268
3.377LysIle: 3.377 ± 0.35
3.698LysLys: 3.698 ± 0.518
4.824LysLeu: 4.824 ± 0.533
1.554LysMet: 1.554 ± 0.263
3.323LysAsn: 3.323 ± 0.446
2.626LysPro: 2.626 ± 0.366
2.412LysGln: 2.412 ± 0.388
2.573LysArg: 2.573 ± 0.367
2.948LysSer: 2.948 ± 0.387
3.538LysThr: 3.538 ± 0.484
2.734LysVal: 2.734 ± 0.452
0.59LysTrp: 0.59 ± 0.162
1.608LysTyr: 1.608 ± 0.279
0.0LysXaa: 0.0 ± 0.0
Leu
8.308LeuAla: 8.308 ± 0.775
1.286LeuCys: 1.286 ± 0.321
3.538LeuAsp: 3.538 ± 0.494
3.966LeuGlu: 3.966 ± 0.551
2.894LeuPhe: 2.894 ± 0.399
4.449LeuGly: 4.449 ± 0.475
1.447LeuHis: 1.447 ± 0.3
3.591LeuIle: 3.591 ± 0.623
4.02LeuLys: 4.02 ± 0.502
6.593LeuLeu: 6.593 ± 0.501
2.198LeuMet: 2.198 ± 0.343
4.502LeuAsn: 4.502 ± 0.475
4.556LeuPro: 4.556 ± 0.504
3.323LeuGln: 3.323 ± 0.704
5.306LeuArg: 5.306 ± 0.472
6.218LeuSer: 6.218 ± 0.695
5.467LeuThr: 5.467 ± 0.52
5.092LeuVal: 5.092 ± 0.424
0.482LeuTrp: 0.482 ± 0.187
1.983LeuTyr: 1.983 ± 0.255
0.0LeuXaa: 0.0 ± 0.0
Met
2.894MetAla: 2.894 ± 0.421
0.161MetCys: 0.161 ± 0.091
1.126MetAsp: 1.126 ± 0.244
1.233MetGlu: 1.233 ± 0.218
0.804MetPhe: 0.804 ± 0.203
1.876MetGly: 1.876 ± 0.311
0.375MetHis: 0.375 ± 0.133
1.018MetIle: 1.018 ± 0.238
1.93MetLys: 1.93 ± 0.271
2.144MetLeu: 2.144 ± 0.307
0.965MetMet: 0.965 ± 0.285
2.037MetAsn: 2.037 ± 0.406
1.983MetPro: 1.983 ± 0.292
0.965MetGln: 0.965 ± 0.225
1.769MetArg: 1.769 ± 0.258
2.144MetSer: 2.144 ± 0.327
2.573MetThr: 2.573 ± 0.275
1.394MetVal: 1.394 ± 0.28
0.214MetTrp: 0.214 ± 0.086
0.643MetTyr: 0.643 ± 0.232
0.0MetXaa: 0.0 ± 0.0
Asn
5.199AsnAla: 5.199 ± 0.519
0.59AsnCys: 0.59 ± 0.213
2.037AsnAsp: 2.037 ± 0.303
2.787AsnGlu: 2.787 ± 0.382
1.662AsnPhe: 1.662 ± 0.346
3.806AsnGly: 3.806 ± 0.434
1.34AsnHis: 1.34 ± 0.288
2.305AsnIle: 2.305 ± 0.395
2.305AsnLys: 2.305 ± 0.365
3.27AsnLeu: 3.27 ± 0.405
1.018AsnMet: 1.018 ± 0.199
1.93AsnAsn: 1.93 ± 0.418
1.93AsnPro: 1.93 ± 0.321
1.983AsnGln: 1.983 ± 0.286
2.305AsnArg: 2.305 ± 0.436
2.412AsnSer: 2.412 ± 0.388
2.037AsnThr: 2.037 ± 0.37
2.626AsnVal: 2.626 ± 0.349
0.697AsnTrp: 0.697 ± 0.205
1.554AsnTyr: 1.554 ± 0.346
0.0AsnXaa: 0.0 ± 0.0
Pro
3.806ProAla: 3.806 ± 0.727
0.482ProCys: 0.482 ± 0.184
3.913ProAsp: 3.913 ± 0.543
4.931ProGlu: 4.931 ± 0.772
1.447ProPhe: 1.447 ± 0.219
3.645ProGly: 3.645 ± 0.612
0.322ProHis: 0.322 ± 0.13
0.858ProIle: 0.858 ± 0.22
2.519ProLys: 2.519 ± 0.885
3.377ProLeu: 3.377 ± 0.458
0.804ProMet: 0.804 ± 0.195
1.018ProAsn: 1.018 ± 0.223
1.394ProPro: 1.394 ± 0.31
2.09ProGln: 2.09 ± 0.595
2.412ProArg: 2.412 ± 0.453
2.68ProSer: 2.68 ± 0.402
2.358ProThr: 2.358 ± 0.349
4.985ProVal: 4.985 ± 0.564
0.536ProTrp: 0.536 ± 0.137
1.662ProTyr: 1.662 ± 0.345
0.0ProXaa: 0.0 ± 0.0
Gln
3.913GlnAla: 3.913 ± 0.584
0.75GlnCys: 0.75 ± 0.233
2.358GlnAsp: 2.358 ± 0.381
2.519GlnGlu: 2.519 ± 0.433
1.34GlnPhe: 1.34 ± 0.265
3.484GlnGly: 3.484 ± 0.885
1.018GlnHis: 1.018 ± 0.229
2.198GlnIle: 2.198 ± 0.455
3.109GlnLys: 3.109 ± 0.428
3.698GlnLeu: 3.698 ± 0.429
0.965GlnMet: 0.965 ± 0.224
1.822GlnAsn: 1.822 ± 0.339
1.822GlnPro: 1.822 ± 0.436
3.323GlnGln: 3.323 ± 0.825
2.519GlnArg: 2.519 ± 0.528
2.68GlnSer: 2.68 ± 0.289
1.662GlnThr: 1.662 ± 0.256
2.358GlnVal: 2.358 ± 0.55
0.59GlnTrp: 0.59 ± 0.153
1.233GlnTyr: 1.233 ± 0.298
0.0GlnXaa: 0.0 ± 0.0
Arg
4.449ArgAla: 4.449 ± 0.488
0.429ArgCys: 0.429 ± 0.174
3.859ArgAsp: 3.859 ± 0.563
5.092ArgGlu: 5.092 ± 0.698
2.948ArgPhe: 2.948 ± 0.448
4.77ArgGly: 4.77 ± 0.79
1.715ArgHis: 1.715 ± 0.253
3.484ArgIle: 3.484 ± 0.519
4.502ArgLys: 4.502 ± 0.603
5.092ArgLeu: 5.092 ± 0.475
1.608ArgMet: 1.608 ± 0.302
3.002ArgAsn: 3.002 ± 0.401
2.037ArgPro: 2.037 ± 0.333
2.734ArgGln: 2.734 ± 0.354
5.146ArgArg: 5.146 ± 0.584
4.02ArgSer: 4.02 ± 0.438
3.538ArgThr: 3.538 ± 0.53
3.538ArgVal: 3.538 ± 0.428
1.179ArgTrp: 1.179 ± 0.221
2.037ArgTyr: 2.037 ± 0.453
0.0ArgXaa: 0.0 ± 0.0
Ser
6.11SerAla: 6.11 ± 0.573
0.858SerCys: 0.858 ± 0.292
3.966SerAsp: 3.966 ± 0.416
4.181SerGlu: 4.181 ± 0.386
2.198SerPhe: 2.198 ± 0.356
5.521SerGly: 5.521 ± 0.623
1.018SerHis: 1.018 ± 0.219
2.412SerIle: 2.412 ± 0.305
2.787SerLys: 2.787 ± 0.406
6.271SerLeu: 6.271 ± 0.759
1.983SerMet: 1.983 ± 0.303
2.144SerAsn: 2.144 ± 0.38
3.323SerPro: 3.323 ± 0.508
2.948SerGln: 2.948 ± 0.36
4.395SerArg: 4.395 ± 0.54
3.377SerSer: 3.377 ± 0.569
3.484SerThr: 3.484 ± 0.425
4.395SerVal: 4.395 ± 0.485
0.804SerTrp: 0.804 ± 0.195
1.501SerTyr: 1.501 ± 0.393
0.0SerXaa: 0.0 ± 0.0
Thr
5.628ThrAla: 5.628 ± 0.444
0.322ThrCys: 0.322 ± 0.151
3.055ThrAsp: 3.055 ± 0.416
3.752ThrGlu: 3.752 ± 0.407
2.198ThrPhe: 2.198 ± 0.493
5.896ThrGly: 5.896 ± 0.713
0.804ThrHis: 0.804 ± 0.164
3.752ThrIle: 3.752 ± 0.498
2.573ThrLys: 2.573 ± 0.324
4.931ThrLeu: 4.931 ± 0.39
1.233ThrMet: 1.233 ± 0.25
1.501ThrAsn: 1.501 ± 0.261
3.538ThrPro: 3.538 ± 0.363
1.662ThrGln: 1.662 ± 0.359
2.787ThrArg: 2.787 ± 0.389
3.752ThrSer: 3.752 ± 0.471
4.02ThrThr: 4.02 ± 0.545
4.502ThrVal: 4.502 ± 0.523
0.911ThrTrp: 0.911 ± 0.202
0.965ThrTyr: 0.965 ± 0.261
0.0ThrXaa: 0.0 ± 0.0
Val
6.753ValAla: 6.753 ± 0.472
1.179ValCys: 1.179 ± 0.273
3.913ValAsp: 3.913 ± 0.542
3.43ValGlu: 3.43 ± 0.381
2.09ValPhe: 2.09 ± 0.303
3.216ValGly: 3.216 ± 0.549
0.482ValHis: 0.482 ± 0.151
3.43ValIle: 3.43 ± 0.456
3.698ValLys: 3.698 ± 0.417
5.574ValLeu: 5.574 ± 0.595
1.983ValMet: 1.983 ± 0.365
3.645ValAsn: 3.645 ± 0.393
3.002ValPro: 3.002 ± 0.38
2.251ValGln: 2.251 ± 0.336
4.556ValArg: 4.556 ± 1.03
4.985ValSer: 4.985 ± 0.501
4.824ValThr: 4.824 ± 0.498
3.806ValVal: 3.806 ± 0.501
0.75ValTrp: 0.75 ± 0.183
1.822ValTyr: 1.822 ± 0.33
0.0ValXaa: 0.0 ± 0.0
Trp
0.697TrpAla: 0.697 ± 0.164
0.161TrpCys: 0.161 ± 0.102
0.536TrpAsp: 0.536 ± 0.212
0.697TrpGlu: 0.697 ± 0.175
0.75TrpPhe: 0.75 ± 0.186
0.911TrpGly: 0.911 ± 0.239
0.214TrpHis: 0.214 ± 0.099
0.75TrpIle: 0.75 ± 0.239
0.697TrpLys: 0.697 ± 0.186
1.072TrpLeu: 1.072 ± 0.233
0.804TrpMet: 0.804 ± 0.162
0.536TrpAsn: 0.536 ± 0.185
0.59TrpPro: 0.59 ± 0.17
1.018TrpGln: 1.018 ± 0.223
0.911TrpArg: 0.911 ± 0.21
0.697TrpSer: 0.697 ± 0.189
0.643TrpThr: 0.643 ± 0.185
0.911TrpVal: 0.911 ± 0.204
0.322TrpTrp: 0.322 ± 0.152
0.322TrpTyr: 0.322 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.841TyrAla: 2.841 ± 0.329
0.214TyrCys: 0.214 ± 0.123
1.394TyrAsp: 1.394 ± 0.299
1.286TyrGlu: 1.286 ± 0.31
1.233TyrPhe: 1.233 ± 0.234
2.144TyrGly: 2.144 ± 0.389
0.375TyrHis: 0.375 ± 0.156
1.34TyrIle: 1.34 ± 0.263
0.911TyrLys: 0.911 ± 0.187
2.037TyrLeu: 2.037 ± 0.395
0.697TyrMet: 0.697 ± 0.192
1.554TyrAsn: 1.554 ± 0.253
1.501TyrPro: 1.501 ± 0.279
1.662TyrGln: 1.662 ± 0.307
2.251TyrArg: 2.251 ± 0.445
1.447TyrSer: 1.447 ± 0.253
1.662TyrThr: 1.662 ± 0.45
1.983TyrVal: 1.983 ± 0.316
0.858TyrTrp: 0.858 ± 0.204
1.233TyrTyr: 1.233 ± 0.26
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (18658 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski