Candidatus Phytoplasma solani

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Candidatus Phytoplasma; 16SrXII (Stolbur group)

Average proteome isoelectric point is 8.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 631 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A421NUU5|A0A421NUU5_9MOLU Uncharacterized protein OS=Candidatus Phytoplasma solani OX=69896 GN=PSSA1_v1c5670 PE=4 SV=1
MM1 pKa = 7.21QKK3 pKa = 10.55AKK5 pKa = 10.29EE6 pKa = 4.12YY7 pKa = 8.64QTTYY11 pKa = 9.28YY12 pKa = 10.38QPDD15 pKa = 4.18GTTVDD20 pKa = 4.5YY21 pKa = 9.48ITEE24 pKa = 4.52FDD26 pKa = 3.54PSTRR30 pKa = 11.84KK31 pKa = 9.07IVKK34 pKa = 8.61ATIFQQDD41 pKa = 3.5GTTVDD46 pKa = 4.39SISDD50 pKa = 4.02FDD52 pKa = 4.44PSTGEE57 pKa = 3.82PVKK60 pKa = 8.89TTTYY64 pKa = 10.63QDD66 pKa = 3.57DD67 pKa = 3.98GTTVDD72 pKa = 5.78FISDD76 pKa = 4.2FDD78 pKa = 4.33PSTGEE83 pKa = 3.83LVKK86 pKa = 8.92TTFYY90 pKa = 10.99QSDD93 pKa = 3.59GTIFDD98 pKa = 4.82IINFF102 pKa = 3.86

Molecular weight: 11.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A421NUL7|A0A421NUL7_9MOLU Putative transposase OS=Candidatus Phytoplasma solani OX=69896 GN=tra5 PE=4 SV=1
MM1 pKa = 7.09KK2 pKa = 10.0QVHH5 pKa = 5.52YY6 pKa = 10.4CGTGRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.97SAVARR18 pKa = 11.84VILTNGTGQITINTKK33 pKa = 9.66NFEE36 pKa = 4.38SYY38 pKa = 11.02LPLPATRR45 pKa = 11.84LDD47 pKa = 3.43MLQPLTVTDD56 pKa = 3.22KK57 pKa = 11.39RR58 pKa = 11.84EE59 pKa = 4.16AYY61 pKa = 9.84DD62 pKa = 3.2VRR64 pKa = 11.84VNVNGGGLCAQAGAIRR80 pKa = 11.84LGIARR85 pKa = 11.84ALLEE89 pKa = 4.82SIPEE93 pKa = 3.8LRR95 pKa = 11.84TILKK99 pKa = 10.16KK100 pKa = 10.77AGLLTRR106 pKa = 11.84DD107 pKa = 3.44ARR109 pKa = 11.84CVEE112 pKa = 4.03RR113 pKa = 11.84KK114 pKa = 10.02KK115 pKa = 11.23YY116 pKa = 10.13GLKK119 pKa = 9.63KK120 pKa = 10.31ARR122 pKa = 11.84RR123 pKa = 11.84APQFSKK129 pKa = 10.95RR130 pKa = 3.57

Molecular weight: 14.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

631

0

631

190526

29

2236

301.9

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.695 ± 0.095

0.932 ± 0.031

4.599 ± 0.06

6.109 ± 0.134

5.624 ± 0.108

4.313 ± 0.09

1.971 ± 0.047

9.412 ± 0.094

10.891 ± 0.118

10.529 ± 0.107

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.713 ± 0.04

6.902 ± 0.103

3.165 ± 0.056

5.719 ± 0.139

2.793 ± 0.065

5.473 ± 0.055

6.011 ± 0.113

4.764 ± 0.092

0.621 ± 0.026

3.762 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski