Sulfitobacter sp. AM1-D1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Sulfitobacter

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4362 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0WEX3|A0A1J0WEX3_9RHOB Choline-sulfatase OS=Sulfitobacter sp. AM1-D1 OX=1917485 GN=BOO69_05085 PE=3 SV=1
MM1 pKa = 7.99LDD3 pKa = 3.96TFCKK7 pKa = 10.3GAVAVAAATAFWVGAAQADD26 pKa = 4.12NQSVLIMEE34 pKa = 5.13GSFFPSTTYY43 pKa = 11.05AKK45 pKa = 10.45PGDD48 pKa = 3.7NVIFTNLSGQVQYY61 pKa = 11.49LSGPEE66 pKa = 4.19DD67 pKa = 3.25SWEE70 pKa = 4.18SGPIGVDD77 pKa = 2.72ATYY80 pKa = 11.06RR81 pKa = 11.84LNLTHH86 pKa = 6.25STPLTFSNGQDD97 pKa = 3.3GEE99 pKa = 4.51GLVEE103 pKa = 4.9GEE105 pKa = 4.25ISYY108 pKa = 11.2DD109 pKa = 3.52PAPLNDD115 pKa = 3.36

Molecular weight: 12.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0WLZ2|A0A1J0WLZ2_9RHOB 1 2-phenylacetyl-CoA epoxidase subunit A OS=Sulfitobacter sp. AM1-D1 OX=1917485 GN=BOO69_18525 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.34SLSAA44 pKa = 3.83

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4362

0

4362

1380988

36

2149

316.6

34.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.354 ± 0.049

0.883 ± 0.012

6.325 ± 0.029

5.82 ± 0.033

3.674 ± 0.023

8.839 ± 0.039

2.041 ± 0.02

5.096 ± 0.028

2.954 ± 0.027

9.954 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.852 ± 0.016

2.565 ± 0.021

5.099 ± 0.026

3.157 ± 0.02

6.943 ± 0.039

4.98 ± 0.023

5.552 ± 0.024

7.347 ± 0.029

1.358 ± 0.015

2.206 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski