Escherichia phage CJ19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Nouzillyvirus; unclassified Nouzillyvirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H0X528|A0A6H0X528_9CAUD Uncharacterized protein OS=Escherichia phage CJ19 OX=2723904 GN=CJ19_078 PE=4 SV=1
MM1 pKa = 7.42VKK3 pKa = 10.19LFCVKK8 pKa = 9.03NTSKK12 pKa = 9.61TLPFTVNNPYY22 pKa = 10.09SAEE25 pKa = 3.98YY26 pKa = 10.2QGDD29 pKa = 4.1GNYY32 pKa = 10.33KK33 pKa = 10.05IYY35 pKa = 10.64GDD37 pKa = 3.72DD38 pKa = 3.66MTWIFAPIDD47 pKa = 3.6GALVEE52 pKa = 5.97FIIADD57 pKa = 3.49

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H0X4Q4|A0A6H0X4Q4_9CAUD Uncharacterized protein OS=Escherichia phage CJ19 OX=2723904 GN=CJ19_041 PE=4 SV=1
MM1 pKa = 7.95KK2 pKa = 10.13LRR4 pKa = 11.84CVKK7 pKa = 10.19IHH9 pKa = 7.21PGLAMLFNDD18 pKa = 4.39YY19 pKa = 10.6FQVGQVYY26 pKa = 8.26EE27 pKa = 4.2AKK29 pKa = 10.21RR30 pKa = 11.84RR31 pKa = 11.84PNRR34 pKa = 11.84HH35 pKa = 6.19LNVADD40 pKa = 4.84VIDD43 pKa = 4.95GYY45 pKa = 11.83NGLSWYY51 pKa = 10.12AVQKK55 pKa = 9.9GSKK58 pKa = 9.98YY59 pKa = 10.73NLNLASGPAVTFVLVKK75 pKa = 10.0GPRR78 pKa = 11.84RR79 pKa = 11.84LVVTEE84 pKa = 3.83KK85 pKa = 10.73RR86 pKa = 11.84VTNVLNDD93 pKa = 3.12KK94 pKa = 10.74RR95 pKa = 11.84KK96 pKa = 8.37FRR98 pKa = 11.84RR99 pKa = 11.84MCRR102 pKa = 11.84QYY104 pKa = 11.06GKK106 pKa = 10.74LRR108 pKa = 11.84FTEE111 pKa = 4.17PGMFCFIAFAAMKK124 pKa = 10.21KK125 pKa = 9.09NARR128 pKa = 11.84NHH130 pKa = 4.36QRR132 pKa = 3.03

Molecular weight: 15.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

14931

32

1127

189.0

21.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.245 ± 0.392

1.339 ± 0.17

6.235 ± 0.198

6.396 ± 0.293

3.931 ± 0.161

7.782 ± 0.257

1.855 ± 0.211

6.557 ± 0.176

7.079 ± 0.373

6.624 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.041 ± 0.216

4.949 ± 0.306

3.061 ± 0.172

3.764 ± 0.209

4.715 ± 0.249

6.631 ± 0.338

5.244 ± 0.242

7.287 ± 0.273

1.507 ± 0.107

3.724 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski