Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia;

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4708 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0LC95|G0LC95_ZOBGA Uncharacterized protein OS=Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij) OX=63186 GN=zobellia_2605 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.41GFKK5 pKa = 10.45YY6 pKa = 10.88YY7 pKa = 10.14IGTITIFSIMTCSLISCSNDD27 pKa = 3.35DD28 pKa = 5.88DD29 pKa = 5.36DD30 pKa = 7.22DD31 pKa = 4.8EE32 pKa = 5.41YY33 pKa = 11.55LGNWVGRR40 pKa = 11.84SVFDD44 pKa = 3.46GSPRR48 pKa = 11.84SGTSSFTIGNMGYY61 pKa = 8.46TGVGYY66 pKa = 10.68DD67 pKa = 3.57GDD69 pKa = 5.04DD70 pKa = 3.62YY71 pKa = 11.4LTSFWSYY78 pKa = 12.15DD79 pKa = 3.04MDD81 pKa = 5.72GDD83 pKa = 4.27FWSQKK88 pKa = 10.74ADD90 pKa = 4.28FIGSARR96 pKa = 11.84NAAVGFEE103 pKa = 4.14VDD105 pKa = 3.08GKK107 pKa = 11.09GYY109 pKa = 10.2IGSGYY114 pKa = 10.68DD115 pKa = 5.01GLDD118 pKa = 3.48EE119 pKa = 6.17LNDD122 pKa = 4.39FYY124 pKa = 11.59AYY126 pKa = 10.39DD127 pKa = 4.02PSSNSWQEE135 pKa = 3.81IASLPSTGRR144 pKa = 11.84RR145 pKa = 11.84SAVAFGMNGFGYY157 pKa = 10.39FGTGFDD163 pKa = 4.34GDD165 pKa = 3.78NDD167 pKa = 3.98RR168 pKa = 11.84KK169 pKa = 10.91DD170 pKa = 3.11FWKK173 pKa = 10.64YY174 pKa = 11.1DD175 pKa = 3.55PSTDD179 pKa = 2.98SWSEE183 pKa = 3.65LVGFGGDD190 pKa = 3.25KK191 pKa = 10.27RR192 pKa = 11.84RR193 pKa = 11.84SATTFTIGDD202 pKa = 3.7KK203 pKa = 10.82VYY205 pKa = 11.06LGTGVSNGIYY215 pKa = 10.32LDD217 pKa = 4.47DD218 pKa = 4.53FWAFDD223 pKa = 3.73ATSEE227 pKa = 4.09TWTKK231 pKa = 11.12KK232 pKa = 10.48LDD234 pKa = 3.73LDD236 pKa = 4.33EE237 pKa = 5.31EE238 pKa = 4.88DD239 pKa = 5.06DD240 pKa = 4.18YY241 pKa = 11.99SITRR245 pKa = 11.84SNAVGFTLNGYY256 pKa = 10.14GYY258 pKa = 8.96IACGTSSGTLASVWQYY274 pKa = 11.92DD275 pKa = 3.53PSTDD279 pKa = 3.1EE280 pKa = 4.53WEE282 pKa = 4.3LKK284 pKa = 9.96TDD286 pKa = 4.07FEE288 pKa = 4.65GTSRR292 pKa = 11.84QDD294 pKa = 2.87AVVFSNGSKK303 pKa = 10.5AVVGLGRR310 pKa = 11.84TGSLYY315 pKa = 10.9LDD317 pKa = 4.14DD318 pKa = 5.85LYY320 pKa = 11.51EE321 pKa = 4.15FFPFDD326 pKa = 4.08EE327 pKa = 4.99YY328 pKa = 11.59DD329 pKa = 4.75DD330 pKa = 4.28EE331 pKa = 4.99DD332 pKa = 3.69

Molecular weight: 36.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0L0S1|G0L0S1_ZOBGA GntR-type transcriptional regulator OS=Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij) OX=63186 GN=zobellia_329 PE=4 SV=1
MM1 pKa = 7.47NKK3 pKa = 9.56NLGYY7 pKa = 8.81TGYY10 pKa = 9.09WAEE13 pKa = 4.2RR14 pKa = 11.84RR15 pKa = 11.84FGFDD19 pKa = 2.25KK20 pKa = 10.71CRR22 pKa = 11.84YY23 pKa = 8.34KK24 pKa = 10.47VQEE27 pKa = 4.52GIFEE31 pKa = 4.22IFRR34 pKa = 11.84AIRR37 pKa = 11.84GVFGMLVRR45 pKa = 11.84RR46 pKa = 11.84FALISGFQPNLISLIKK62 pKa = 10.51LL63 pKa = 3.44

Molecular weight: 7.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4708

0

4708

1661998

25

6203

353.0

39.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.675 ± 0.029

0.739 ± 0.011

6.01 ± 0.054

6.659 ± 0.033

5.028 ± 0.025

7.096 ± 0.035

1.853 ± 0.021

6.995 ± 0.031

7.219 ± 0.053

9.233 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.22 ± 0.02

5.605 ± 0.036

3.74 ± 0.024

3.272 ± 0.018

3.754 ± 0.024

6.501 ± 0.029

5.77 ± 0.046

6.327 ± 0.029

1.197 ± 0.013

4.107 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski