Tenacibaculum todarodis
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2636 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3JGQ9|A0A1L3JGQ9_9FLAO Uncharacterized protein OS=Tenacibaculum todarodis OX=1850252 GN=LPB136_02460 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.37 KK3 pKa = 10.18 LLPLIFLFLTFNLFAQKK20 pKa = 9.71 EE21 pKa = 4.13 ANIWYY26 pKa = 9.23 FGRR29 pKa = 11.84 NAGIDD34 pKa = 3.78 FSTTPPTALTDD45 pKa = 3.84 GQLNTLEE52 pKa = 4.77 GCSSFSDD59 pKa = 3.79 SNGNLLFYY67 pKa = 11.05 VGAPNSADD75 pKa = 3.13 NRR77 pKa = 11.84 LTVWDD82 pKa = 4.11 KK83 pKa = 11.06 NHH85 pKa = 7.17 NIMNYY90 pKa = 8.77 TNGNIGNNLLGHH102 pKa = 6.25 TSSTQSAMIIPKK114 pKa = 9.87 PEE116 pKa = 3.9 STTIFYY122 pKa = 10.85 LFTVDD127 pKa = 5.49 DD128 pKa = 4.96 GPGSGGAEE136 pKa = 3.81 GMNLYY141 pKa = 9.5 TIDD144 pKa = 3.42 MSLNGGLGQLIDD156 pKa = 4.13 EE157 pKa = 5.56 DD158 pKa = 4.57 GDD160 pKa = 3.85 GDD162 pKa = 4.24 FFEE165 pKa = 5.57 DD166 pKa = 4.89 LSDD169 pKa = 3.53 IRR171 pKa = 11.84 KK172 pKa = 9.56 NDD174 pKa = 3.03 WTEE177 pKa = 3.24 KK178 pKa = 8.59 VAAVRR183 pKa = 11.84 GANCNEE189 pKa = 3.71 FWVVSVVNNTFFSYY203 pKa = 10.38 QVTSSGVNLTPVTSPVSTPAQRR225 pKa = 11.84 RR226 pKa = 11.84 GYY228 pKa = 10.71 LKK230 pKa = 10.79 LSPDD234 pKa = 3.37 GSKK237 pKa = 10.66 LAIANQDD244 pKa = 3.4 SNAMVYY250 pKa = 10.63 SFDD253 pKa = 3.56 NSTGRR258 pKa = 11.84 VSNDD262 pKa = 3.47 GIILLNTGGDD272 pKa = 3.73 GQPYY276 pKa = 9.56 GVEE279 pKa = 4.03 FSIDD283 pKa = 3.38 SKK285 pKa = 11.32 KK286 pKa = 11.01 LYY288 pKa = 10.36 FSTVSSFRR296 pKa = 11.84 QDD298 pKa = 3.0 LSEE301 pKa = 4.58 PEE303 pKa = 3.52 VTYY306 pKa = 11.04 RR307 pKa = 11.84 LFQFDD312 pKa = 5.94 LIATDD317 pKa = 3.63 IPGSKK322 pKa = 10.17 SEE324 pKa = 4.33 IYY326 pKa = 10.43 RR327 pKa = 11.84 NNNGFRR333 pKa = 11.84 GALQLGPDD341 pKa = 3.48 GRR343 pKa = 11.84 IYY345 pKa = 10.56 ATIPQAYY352 pKa = 9.11 DD353 pKa = 3.65 DD354 pKa = 4.79 PNGDD358 pKa = 3.5 ARR360 pKa = 11.84 FLSVIEE366 pKa = 4.44 NPTANATDD374 pKa = 4.14 IIFTQNAIDD383 pKa = 3.78 LGGRR387 pKa = 11.84 TATQGLPPFISSLLLGIEE405 pKa = 4.43 LTDD408 pKa = 3.37 TTNNQIISDD417 pKa = 4.48 EE418 pKa = 3.98 IVQYY422 pKa = 11.12 CLTEE426 pKa = 4.44 NILIQPEE433 pKa = 4.22 AVTPQTGTTVDD444 pKa = 4.01 YY445 pKa = 10.7 EE446 pKa = 4.21 WTFDD450 pKa = 4.26 DD451 pKa = 4.52 GTTTSIISTTRR462 pKa = 11.84 DD463 pKa = 3.02 LTLNNIQFNNAGEE476 pKa = 4.19 YY477 pKa = 9.64 TLTVTLTDD485 pKa = 3.31 EE486 pKa = 4.97 CGNPTKK492 pKa = 11.03 LEE494 pKa = 3.96 GTFTIEE500 pKa = 4.17 VYY502 pKa = 11.3 NNLTPVAPTDD512 pKa = 4.44 INFCDD517 pKa = 4.39 DD518 pKa = 5.49 DD519 pKa = 6.71 GDD521 pKa = 4.03 GLQAFDD527 pKa = 4.52 FQTDD531 pKa = 3.96 LTPTILNGQDD541 pKa = 3.2 PTLVEE546 pKa = 3.92 VVYY549 pKa = 11.1 YY550 pKa = 8.24 PTEE553 pKa = 3.86 NDD555 pKa = 3.54 ALNNTAPITNPFTPTAFYY573 pKa = 10.64 DD574 pKa = 3.82 GPIWVRR580 pKa = 11.84 VHH582 pKa = 5.75 NQRR585 pKa = 11.84 APNACNEE592 pKa = 4.21 VYY594 pKa = 10.72 SFNLQITGEE603 pKa = 4.3 PVAQTPDD610 pKa = 4.57 DD611 pKa = 3.85 IVACDD616 pKa = 3.66 GDD618 pKa = 4.17 GTDD621 pKa = 5.12 SVDD624 pKa = 4.59 NDD626 pKa = 3.37 GLYY629 pKa = 11.1 DD630 pKa = 3.82 NFILNTRR637 pKa = 11.84 DD638 pKa = 3.5 AQILGALSEE647 pKa = 4.34 TQYY650 pKa = 10.32 TVSYY654 pKa = 8.11 HH655 pKa = 5.96 TSLADD660 pKa = 3.5 AQTSATTNAIDD671 pKa = 4.85 KK672 pKa = 7.54 NTNYY676 pKa = 10.14 QSGNDD681 pKa = 3.53 IIFVRR686 pKa = 11.84 VEE688 pKa = 3.63 NVDD691 pKa = 3.59 NVNCNDD697 pKa = 3.43 TSQSFEE703 pKa = 5.16 LIVNPLPTPVDD714 pKa = 3.74 VTILQCDD721 pKa = 3.67 DD722 pKa = 4.48 DD723 pKa = 6.7 ADD725 pKa = 4.06 LVADD729 pKa = 3.96 INLTLEE735 pKa = 4.1 QEE737 pKa = 4.65 NISTNHH743 pKa = 4.96 TNEE746 pKa = 3.63 TFEE749 pKa = 4.34 YY750 pKa = 8.49 FTTQAEE756 pKa = 4.75 AIAGTPVIANPIAYY770 pKa = 8.22 NASNGDD776 pKa = 3.78 QVWVRR781 pKa = 11.84 TISTEE786 pKa = 3.21 NCYY789 pKa = 10.48 RR790 pKa = 11.84 ITTIDD795 pKa = 3.54 IIISFASDD803 pKa = 3.07 VPYY806 pKa = 10.68 NRR808 pKa = 11.84 NFIEE812 pKa = 5.29 CDD814 pKa = 3.32 DD815 pKa = 4.44 FLDD818 pKa = 5.25 LDD820 pKa = 4.54 GNDD823 pKa = 3.61 TPGANDD829 pKa = 3.75 DD830 pKa = 3.82 TDD832 pKa = 4.98 GIATFDD838 pKa = 3.74 FSAATSEE845 pKa = 4.34 ILGHH849 pKa = 6.69 PSLAGALNLDD859 pKa = 3.35 VKK861 pKa = 10.89 YY862 pKa = 10.83 FEE864 pKa = 5.21 TIADD868 pKa = 4.06 RR869 pKa = 11.84 ASEE872 pKa = 4.05 INEE875 pKa = 3.53 ISDD878 pKa = 3.86 ISNHH882 pKa = 6.13 RR883 pKa = 11.84 NNNDD887 pKa = 3.37 PAYY890 pKa = 10.63 ANLQTIYY897 pKa = 11.0 AKK899 pKa = 10.38 IINTVNNDD907 pKa = 3.4 CTGLAEE913 pKa = 4.68 LTLQTTPVPVANAINPDD930 pKa = 3.61 NEE932 pKa = 4.32 YY933 pKa = 10.51 EE934 pKa = 4.1 LCDD937 pKa = 4.52 DD938 pKa = 5.14 FVDD941 pKa = 4.1 ADD943 pKa = 3.87 GSNGIIQSFDD953 pKa = 3.88 LEE955 pKa = 4.45 SQTAAILGSQDD966 pKa = 3.06 PLAYY970 pKa = 9.46 TITYY974 pKa = 9.58 HH975 pKa = 7.06 EE976 pKa = 4.53 SAADD980 pKa = 3.61 ATSGANPLASPYY992 pKa = 10.84 TNITRR997 pKa = 11.84 DD998 pKa = 3.09 RR999 pKa = 11.84 QTIYY1003 pKa = 11.26 ARR1005 pKa = 11.84 VSGNGCYY1012 pKa = 9.68 FDD1014 pKa = 5.42 RR1015 pKa = 11.84 YY1016 pKa = 10.82 SFDD1019 pKa = 2.99 IVVNSLPIANQLNMANTLEE1038 pKa = 4.19 VCDD1041 pKa = 5.14 DD1042 pKa = 4.41 DD1043 pKa = 7.03 ADD1045 pKa = 4.1 GSATNGFVQNIDD1057 pKa = 4.01 LEE1059 pKa = 4.66 TLTTTILGTQDD1070 pKa = 3.18 PTNFTVTYY1078 pKa = 7.19 HH1079 pKa = 5.98 TSYY1082 pKa = 11.87 ALAQTGAIPITGMYY1096 pKa = 10.63 SNTTQGGDD1104 pKa = 3.14 TVYY1107 pKa = 10.95 VRR1109 pKa = 11.84 IVNNTTTCVNDD1120 pKa = 3.49 TFNFQVLINAEE1131 pKa = 4.09 PLANPDD1137 pKa = 3.65 NLVSNLSEE1145 pKa = 4.66 CDD1147 pKa = 3.19 NDD1149 pKa = 5.25 ADD1151 pKa = 4.81 GDD1153 pKa = 4.11 DD1154 pKa = 4.32 ANGFVQSFDD1163 pKa = 4.47 LEE1165 pKa = 4.36 SQIPDD1170 pKa = 3.08 ILGDD1174 pKa = 3.53 PTVQDD1179 pKa = 3.37 EE1180 pKa = 4.74 DD1181 pKa = 4.08 DD1182 pKa = 4.39 FNVTFHH1188 pKa = 6.79 LTQADD1193 pKa = 3.92 ATDD1196 pKa = 4.2 GSAPQSSPFTNTDD1209 pKa = 2.6 INQQTIFVRR1218 pKa = 11.84 IEE1220 pKa = 3.74 NKK1222 pKa = 9.25 ATGCVNDD1229 pKa = 5.17 DD1230 pKa = 3.54 LTFDD1234 pKa = 4.17 VIVNSLPDD1242 pKa = 3.68 FVVTSPQIVCLNNPPLTLTVEE1263 pKa = 4.35 NPNDD1267 pKa = 3.14 VYY1269 pKa = 10.84 TYY1271 pKa = 10.46 QWFDD1275 pKa = 2.92 ASGNPLGTAITQDD1288 pKa = 3.33 VTVGGDD1294 pKa = 3.53 YY1295 pKa = 11.01 SVTATTTNGTMCPRR1309 pKa = 11.84 TRR1311 pKa = 11.84 TITVNEE1317 pKa = 4.23 SNIATITDD1325 pKa = 3.7 DD1326 pKa = 5.93 DD1327 pKa = 3.81 ITIVDD1332 pKa = 3.86 DD1333 pKa = 4.41 SEE1335 pKa = 4.67 NNSITINDD1343 pKa = 3.89 SNLGEE1348 pKa = 4.26 GDD1350 pKa = 3.89 YY1351 pKa = 11.29 EE1352 pKa = 4.31 FALIDD1357 pKa = 3.77 EE1358 pKa = 4.75 NGFTIVNYY1366 pKa = 9.71 QDD1368 pKa = 3.31 EE1369 pKa = 4.72 AFFDD1373 pKa = 4.08 GLNGGIYY1380 pKa = 9.41 TIHH1383 pKa = 7.17 IRR1385 pKa = 11.84 DD1386 pKa = 3.61 KK1387 pKa = 10.47 NGCGVTTHH1395 pKa = 6.44 TVPVIEE1401 pKa = 4.59 FPKK1404 pKa = 10.59 FFTPNNDD1411 pKa = 3.67 GIKK1414 pKa = 10.46 DD1415 pKa = 3.26 VWTIKK1420 pKa = 9.08 GTNATFFPINTVVIFNRR1437 pKa = 11.84 QGKK1440 pKa = 9.7 IINQFNINAYY1450 pKa = 9.53 GWDD1453 pKa = 3.26 GHH1455 pKa = 6.19 YY1456 pKa = 10.1 KK1457 pKa = 10.35 DD1458 pKa = 5.42 KK1459 pKa = 11.46 AMPSNNYY1466 pKa = 7.47 WFKK1469 pKa = 10.95 ATLVDD1474 pKa = 3.57 AKK1476 pKa = 10.84 GITRR1480 pKa = 11.84 TRR1482 pKa = 11.84 SGYY1485 pKa = 9.96 FSLIRR1490 pKa = 11.84 RR1491 pKa = 4.0
Molecular weight: 162.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.745
IPC_protein 3.795
Toseland 3.567
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.465
Solomon 3.795
Lehninger 3.757
Nozaki 3.897
DTASelect 4.19
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.923
Patrickios 0.884
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A1L3JFN5|A0A1L3JFN5_9FLAO Uncharacterized protein OS=Tenacibaculum todarodis OX=1850252 GN=LPB136_00550 PE=4 SV=1
MM1 pKa = 7.54 SVRR4 pKa = 11.84 KK5 pKa = 9.63 LKK7 pKa = 10.48 PVTPGQRR14 pKa = 11.84 FRR16 pKa = 11.84 VVNGFDD22 pKa = 5.49 AITTDD27 pKa = 3.56 KK28 pKa = 11.07 PEE30 pKa = 4.39 KK31 pKa = 10.58 SLLAPNKK38 pKa = 10.02 RR39 pKa = 11.84 SGGRR43 pKa = 11.84 NSQGRR48 pKa = 11.84 MTTRR52 pKa = 11.84 NIGGGHH58 pKa = 4.58 KK59 pKa = 9.49 QKK61 pKa = 10.8 YY62 pKa = 9.64 RR63 pKa = 11.84 IIDD66 pKa = 3.67 FKK68 pKa = 10.99 KK69 pKa = 10.17 DD70 pKa = 3.34 KK71 pKa = 10.76 QGIPATVKK79 pKa = 10.01 TIEE82 pKa = 4.0 YY83 pKa = 9.86 DD84 pKa = 3.47 PNRR87 pKa = 11.84 TAFIALVFYY96 pKa = 11.16 ADD98 pKa = 3.36 GEE100 pKa = 4.23 KK101 pKa = 10.28 RR102 pKa = 11.84 YY103 pKa = 10.74 VIAQNGLKK111 pKa = 10.19 VGQTIVSGRR120 pKa = 11.84 EE121 pKa = 3.72 ATPEE125 pKa = 3.74 IGNALYY131 pKa = 10.83 LSDD134 pKa = 4.56 IPLGTTISCIEE145 pKa = 4.03 LRR147 pKa = 11.84 PGQGAVMARR156 pKa = 11.84 SAGSFAQLMARR167 pKa = 11.84 DD168 pKa = 3.96 GKK170 pKa = 10.52 YY171 pKa = 9.12 ATVKK175 pKa = 10.42 LPSGEE180 pKa = 4.06 TRR182 pKa = 11.84 LILLTCMATIGVVSNSDD199 pKa = 3.21 HH200 pKa = 6.09 QLLVSGKK207 pKa = 9.49 AGRR210 pKa = 11.84 SRR212 pKa = 11.84 WLGRR216 pKa = 11.84 RR217 pKa = 11.84 PRR219 pKa = 11.84 TNPVRR224 pKa = 11.84 MNPVDD229 pKa = 3.43 HH230 pKa = 7.07 PMGGGEE236 pKa = 3.89 GRR238 pKa = 11.84 ASGGHH243 pKa = 5.13 PRR245 pKa = 11.84 SRR247 pKa = 11.84 NGIPAKK253 pKa = 10.28 GYY255 pKa = 7.21 KK256 pKa = 8.8 TRR258 pKa = 11.84 SKK260 pKa = 9.63 TKK262 pKa = 10.22 ASNKK266 pKa = 9.95 YY267 pKa = 9.56 IIEE270 pKa = 4.06 RR271 pKa = 11.84 RR272 pKa = 11.84 KK273 pKa = 10.1 KK274 pKa = 9.82
Molecular weight: 29.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.911
IPC_protein 10.745
Toseland 10.994
ProMoST 10.73
Dawson 11.052
Bjellqvist 10.774
Wikipedia 11.286
Rodwell 11.345
Grimsley 11.096
Solomon 11.213
Lehninger 11.184
Nozaki 10.965
DTASelect 10.774
Thurlkill 10.979
EMBOSS 11.403
Sillero 10.994
Patrickios 11.052
IPC_peptide 11.228
IPC2_peptide 9.663
IPC2.peptide.svr19 8.492
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2636
0
2636
903262
50
6670
342.7
38.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.134 ± 0.055
0.686 ± 0.016
5.419 ± 0.079
6.669 ± 0.053
5.385 ± 0.069
6.204 ± 0.053
1.599 ± 0.029
8.137 ± 0.054
8.714 ± 0.112
9.063 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.003 ± 0.034
6.674 ± 0.063
3.199 ± 0.039
3.221 ± 0.032
3.064 ± 0.044
6.506 ± 0.047
6.269 ± 0.166
6.285 ± 0.06
0.943 ± 0.019
3.824 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here