Amino acid dipepetide frequency for Lactobacillus phage CL1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.673AlaAla: 8.673 ± 1.187
0.413AlaCys: 0.413 ± 0.169
6.69AlaAsp: 6.69 ± 0.896
5.204AlaGlu: 5.204 ± 0.771
4.047AlaPhe: 4.047 ± 0.667
5.121AlaGly: 5.121 ± 1.038
1.239AlaHis: 1.239 ± 0.343
5.369AlaIle: 5.369 ± 0.949
5.451AlaLys: 5.451 ± 0.784
6.773AlaLeu: 6.773 ± 0.689
2.561AlaMet: 2.561 ± 0.544
5.451AlaAsn: 5.451 ± 1.016
1.487AlaPro: 1.487 ± 0.349
3.304AlaGln: 3.304 ± 0.607
3.799AlaArg: 3.799 ± 0.729
5.534AlaSer: 5.534 ± 0.743
5.947AlaThr: 5.947 ± 0.83
6.195AlaVal: 6.195 ± 1.062
1.239AlaTrp: 1.239 ± 0.457
3.139AlaTyr: 3.139 ± 0.622
0.0AlaXaa: 0.0 ± 0.0
Cys
0.413CysAla: 0.413 ± 0.185
0.083CysCys: 0.083 ± 0.082
0.33CysAsp: 0.33 ± 0.154
0.413CysGlu: 0.413 ± 0.195
0.248CysPhe: 0.248 ± 0.14
0.661CysGly: 0.661 ± 0.299
0.0CysHis: 0.0 ± 0.0
0.248CysIle: 0.248 ± 0.133
0.413CysLys: 0.413 ± 0.202
0.578CysLeu: 0.578 ± 0.26
0.0CysMet: 0.0 ± 0.0
0.165CysAsn: 0.165 ± 0.195
0.248CysPro: 0.248 ± 0.149
0.083CysGln: 0.083 ± 0.092
0.165CysArg: 0.165 ± 0.113
0.33CysSer: 0.33 ± 0.178
0.165CysThr: 0.165 ± 0.129
0.413CysVal: 0.413 ± 0.198
0.248CysTrp: 0.248 ± 0.154
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.543AspAla: 4.543 ± 0.683
0.083AspCys: 0.083 ± 0.084
5.782AspAsp: 5.782 ± 1.14
4.378AspGlu: 4.378 ± 0.938
2.561AspPhe: 2.561 ± 0.419
7.103AspGly: 7.103 ± 1.127
1.569AspHis: 1.569 ± 0.35
3.304AspIle: 3.304 ± 0.577
4.956AspLys: 4.956 ± 1.215
4.708AspLeu: 4.708 ± 0.595
1.735AspMet: 1.735 ± 0.311
2.973AspAsn: 2.973 ± 0.615
2.726AspPro: 2.726 ± 0.488
2.726AspGln: 2.726 ± 0.585
3.139AspArg: 3.139 ± 0.621
4.378AspSer: 4.378 ± 0.58
2.808AspThr: 2.808 ± 0.687
3.634AspVal: 3.634 ± 0.472
1.074AspTrp: 1.074 ± 0.245
2.973AspTyr: 2.973 ± 0.582
0.0AspXaa: 0.0 ± 0.0
Glu
3.882GluAla: 3.882 ± 0.668
0.165GluCys: 0.165 ± 0.117
2.891GluAsp: 2.891 ± 0.578
2.395GluGlu: 2.395 ± 0.561
1.404GluPhe: 1.404 ± 0.332
3.882GluGly: 3.882 ± 0.625
0.991GluHis: 0.991 ± 0.237
3.634GluIle: 3.634 ± 0.552
3.386GluLys: 3.386 ± 0.706
5.038GluLeu: 5.038 ± 0.714
1.817GluMet: 1.817 ± 0.468
3.056GluAsn: 3.056 ± 0.534
1.487GluPro: 1.487 ± 0.367
2.478GluGln: 2.478 ± 0.448
2.891GluArg: 2.891 ± 0.51
2.808GluSer: 2.808 ± 0.494
3.221GluThr: 3.221 ± 0.457
4.047GluVal: 4.047 ± 0.527
0.909GluTrp: 0.909 ± 0.268
2.065GluTyr: 2.065 ± 0.446
0.0GluXaa: 0.0 ± 0.0
Phe
2.973PheAla: 2.973 ± 0.512
0.165PheCys: 0.165 ± 0.167
2.478PheAsp: 2.478 ± 0.345
1.569PheGlu: 1.569 ± 0.33
2.065PhePhe: 2.065 ± 0.558
2.726PheGly: 2.726 ± 0.478
0.413PheHis: 0.413 ± 0.199
2.23PheIle: 2.23 ± 0.368
2.973PheLys: 2.973 ± 0.532
2.643PheLeu: 2.643 ± 0.575
1.239PheMet: 1.239 ± 0.313
2.148PheAsn: 2.148 ± 0.354
1.487PhePro: 1.487 ± 0.437
0.991PheGln: 0.991 ± 0.326
1.239PheArg: 1.239 ± 0.4
3.552PheSer: 3.552 ± 0.902
3.139PheThr: 3.139 ± 0.583
2.478PheVal: 2.478 ± 0.471
0.413PheTrp: 0.413 ± 0.218
1.156PheTyr: 1.156 ± 0.291
0.0PheXaa: 0.0 ± 0.0
Gly
4.956GlyAla: 4.956 ± 0.953
0.083GlyCys: 0.083 ± 0.079
4.791GlyAsp: 4.791 ± 1.251
2.973GlyGlu: 2.973 ± 0.534
2.313GlyPhe: 2.313 ± 0.476
4.212GlyGly: 4.212 ± 0.532
1.239GlyHis: 1.239 ± 0.326
6.69GlyIle: 6.69 ± 1.326
5.864GlyLys: 5.864 ± 0.669
5.121GlyLeu: 5.121 ± 0.973
2.23GlyMet: 2.23 ± 0.384
3.221GlyAsn: 3.221 ± 0.559
1.404GlyPro: 1.404 ± 0.265
2.23GlyGln: 2.23 ± 0.437
2.148GlyArg: 2.148 ± 0.431
5.617GlySer: 5.617 ± 0.744
5.286GlyThr: 5.286 ± 0.757
4.295GlyVal: 4.295 ± 0.628
0.909GlyTrp: 0.909 ± 0.303
2.973GlyTyr: 2.973 ± 0.558
0.0GlyXaa: 0.0 ± 0.0
His
0.826HisAla: 0.826 ± 0.306
0.33HisCys: 0.33 ± 0.178
1.322HisAsp: 1.322 ± 0.346
0.826HisGlu: 0.826 ± 0.254
0.496HisPhe: 0.496 ± 0.214
0.991HisGly: 0.991 ± 0.243
0.496HisHis: 0.496 ± 0.26
0.826HisIle: 0.826 ± 0.28
1.074HisLys: 1.074 ± 0.295
1.322HisLeu: 1.322 ± 0.339
0.33HisMet: 0.33 ± 0.18
0.661HisAsn: 0.661 ± 0.264
0.496HisPro: 0.496 ± 0.216
0.826HisGln: 0.826 ± 0.275
0.496HisArg: 0.496 ± 0.251
1.322HisSer: 1.322 ± 0.451
1.239HisThr: 1.239 ± 0.362
0.826HisVal: 0.826 ± 0.268
0.165HisTrp: 0.165 ± 0.104
1.074HisTyr: 1.074 ± 0.336
0.0HisXaa: 0.0 ± 0.0
Ile
6.443IleAla: 6.443 ± 0.707
0.413IleCys: 0.413 ± 0.207
4.13IleAsp: 4.13 ± 0.545
3.965IleGlu: 3.965 ± 0.723
1.982IlePhe: 1.982 ± 0.434
3.882IleGly: 3.882 ± 0.646
0.909IleHis: 0.909 ± 0.3
3.139IleIle: 3.139 ± 0.581
4.46IleLys: 4.46 ± 0.612
3.139IleLeu: 3.139 ± 0.463
1.404IleMet: 1.404 ± 0.362
3.965IleAsn: 3.965 ± 0.704
2.313IlePro: 2.313 ± 0.381
2.395IleGln: 2.395 ± 0.528
2.23IleArg: 2.23 ± 0.526
4.625IleSer: 4.625 ± 0.709
5.038IleThr: 5.038 ± 0.68
4.46IleVal: 4.46 ± 0.824
1.817IleTrp: 1.817 ± 0.675
1.735IleTyr: 1.735 ± 0.534
0.0IleXaa: 0.0 ± 0.0
Lys
6.938LysAla: 6.938 ± 0.985
0.496LysCys: 0.496 ± 0.197
3.634LysAsp: 3.634 ± 0.66
3.304LysGlu: 3.304 ± 0.526
2.478LysPhe: 2.478 ± 0.588
4.791LysGly: 4.791 ± 1.307
1.239LysHis: 1.239 ± 0.487
4.212LysIle: 4.212 ± 0.527
3.882LysLys: 3.882 ± 0.751
6.69LysLeu: 6.69 ± 0.91
2.23LysMet: 2.23 ± 0.468
3.056LysAsn: 3.056 ± 0.663
2.808LysPro: 2.808 ± 0.609
5.286LysGln: 5.286 ± 0.845
3.469LysArg: 3.469 ± 0.683
3.717LysSer: 3.717 ± 0.58
4.46LysThr: 4.46 ± 0.584
4.13LysVal: 4.13 ± 0.507
0.496LysTrp: 0.496 ± 0.232
2.395LysTyr: 2.395 ± 0.449
0.0LysXaa: 0.0 ± 0.0
Leu
6.773LeuAla: 6.773 ± 0.859
0.413LeuCys: 0.413 ± 0.173
4.625LeuAsp: 4.625 ± 0.662
3.717LeuGlu: 3.717 ± 0.52
2.478LeuPhe: 2.478 ± 0.468
4.13LeuGly: 4.13 ± 0.669
0.991LeuHis: 0.991 ± 0.318
5.369LeuIle: 5.369 ± 0.746
5.451LeuLys: 5.451 ± 0.921
5.451LeuLeu: 5.451 ± 0.587
2.065LeuMet: 2.065 ± 0.429
4.295LeuAsn: 4.295 ± 0.689
2.808LeuPro: 2.808 ± 0.494
3.965LeuGln: 3.965 ± 0.501
3.386LeuArg: 3.386 ± 0.583
6.195LeuSer: 6.195 ± 0.774
5.782LeuThr: 5.782 ± 0.565
4.543LeuVal: 4.543 ± 0.691
1.569LeuTrp: 1.569 ± 0.544
2.643LeuTyr: 2.643 ± 0.545
0.0LeuXaa: 0.0 ± 0.0
Met
2.148MetAla: 2.148 ± 0.482
0.0MetCys: 0.0 ± 0.0
1.156MetAsp: 1.156 ± 0.345
1.156MetGlu: 1.156 ± 0.294
0.413MetPhe: 0.413 ± 0.165
2.065MetGly: 2.065 ± 0.386
0.33MetHis: 0.33 ± 0.153
1.735MetIle: 1.735 ± 0.446
1.982MetLys: 1.982 ± 0.401
2.148MetLeu: 2.148 ± 0.498
0.826MetMet: 0.826 ± 0.274
2.23MetAsn: 2.23 ± 0.429
1.074MetPro: 1.074 ± 0.269
1.487MetGln: 1.487 ± 0.406
1.074MetArg: 1.074 ± 0.308
1.652MetSer: 1.652 ± 0.392
2.726MetThr: 2.726 ± 0.403
1.817MetVal: 1.817 ± 0.538
0.248MetTrp: 0.248 ± 0.195
0.909MetTyr: 0.909 ± 0.273
0.0MetXaa: 0.0 ± 0.0
Asn
5.038AsnAla: 5.038 ± 0.594
0.413AsnCys: 0.413 ± 0.2
4.46AsnAsp: 4.46 ± 0.663
2.643AsnGlu: 2.643 ± 0.498
1.9AsnPhe: 1.9 ± 0.439
5.534AsnGly: 5.534 ± 0.747
1.074AsnHis: 1.074 ± 0.319
2.395AsnIle: 2.395 ± 0.441
3.304AsnLys: 3.304 ± 0.678
3.469AsnLeu: 3.469 ± 0.423
1.652AsnMet: 1.652 ± 0.418
2.561AsnAsn: 2.561 ± 0.427
2.726AsnPro: 2.726 ± 0.394
2.561AsnGln: 2.561 ± 0.484
2.726AsnArg: 2.726 ± 0.477
3.221AsnSer: 3.221 ± 0.679
2.478AsnThr: 2.478 ± 0.729
2.726AsnVal: 2.726 ± 0.394
0.743AsnTrp: 0.743 ± 0.228
2.395AsnTyr: 2.395 ± 0.622
0.0AsnXaa: 0.0 ± 0.0
Pro
2.808ProAla: 2.808 ± 0.447
0.083ProCys: 0.083 ± 0.08
2.478ProAsp: 2.478 ± 0.481
3.056ProGlu: 3.056 ± 0.7
1.074ProPhe: 1.074 ± 0.319
1.322ProGly: 1.322 ± 0.357
0.496ProHis: 0.496 ± 0.199
2.395ProIle: 2.395 ± 0.378
3.386ProLys: 3.386 ± 0.612
1.9ProLeu: 1.9 ± 0.427
0.661ProMet: 0.661 ± 0.22
1.404ProAsn: 1.404 ± 0.365
0.248ProPro: 0.248 ± 0.137
1.322ProGln: 1.322 ± 0.291
0.826ProArg: 0.826 ± 0.261
2.726ProSer: 2.726 ± 0.571
1.817ProThr: 1.817 ± 0.394
2.726ProVal: 2.726 ± 0.504
0.826ProTrp: 0.826 ± 0.221
1.156ProTyr: 1.156 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
4.873GlnAla: 4.873 ± 0.601
0.248GlnCys: 0.248 ± 0.157
2.23GlnAsp: 2.23 ± 0.513
2.561GlnGlu: 2.561 ± 0.541
1.735GlnPhe: 1.735 ± 0.338
2.478GlnGly: 2.478 ± 0.452
0.909GlnHis: 0.909 ± 0.256
2.478GlnIle: 2.478 ± 0.458
2.395GlnLys: 2.395 ± 0.393
4.13GlnLeu: 4.13 ± 0.564
1.322GlnMet: 1.322 ± 0.326
2.065GlnAsn: 2.065 ± 0.394
1.487GlnPro: 1.487 ± 0.292
2.726GlnGln: 2.726 ± 0.485
1.9GlnArg: 1.9 ± 0.414
2.561GlnSer: 2.561 ± 0.441
3.139GlnThr: 3.139 ± 0.612
3.386GlnVal: 3.386 ± 0.535
1.239GlnTrp: 1.239 ± 0.241
2.23GlnTyr: 2.23 ± 0.351
0.0GlnXaa: 0.0 ± 0.0
Arg
2.726ArgAla: 2.726 ± 0.57
0.413ArgCys: 0.413 ± 0.227
1.487ArgAsp: 1.487 ± 0.309
2.643ArgGlu: 2.643 ± 0.448
2.23ArgPhe: 2.23 ± 0.517
2.23ArgGly: 2.23 ± 0.471
0.496ArgHis: 0.496 ± 0.239
2.395ArgIle: 2.395 ± 0.552
3.799ArgLys: 3.799 ± 0.586
3.965ArgLeu: 3.965 ± 0.597
0.991ArgMet: 0.991 ± 0.282
2.561ArgAsn: 2.561 ± 0.414
1.239ArgPro: 1.239 ± 0.267
2.561ArgGln: 2.561 ± 0.551
1.982ArgArg: 1.982 ± 0.538
1.9ArgSer: 1.9 ± 0.355
2.23ArgThr: 2.23 ± 0.358
2.23ArgVal: 2.23 ± 0.461
0.248ArgTrp: 0.248 ± 0.146
2.561ArgTyr: 2.561 ± 0.671
0.0ArgXaa: 0.0 ± 0.0
Ser
5.864SerAla: 5.864 ± 0.866
0.083SerCys: 0.083 ± 0.09
5.534SerAsp: 5.534 ± 0.757
2.891SerGlu: 2.891 ± 0.584
3.386SerPhe: 3.386 ± 0.64
5.864SerGly: 5.864 ± 0.97
0.496SerHis: 0.496 ± 0.202
3.799SerIle: 3.799 ± 0.61
5.617SerLys: 5.617 ± 0.744
4.708SerLeu: 4.708 ± 0.65
1.487SerMet: 1.487 ± 0.345
3.799SerAsn: 3.799 ± 0.569
1.982SerPro: 1.982 ± 0.461
3.304SerGln: 3.304 ± 0.676
2.065SerArg: 2.065 ± 0.389
6.03SerSer: 6.03 ± 0.913
4.543SerThr: 4.543 ± 0.681
4.13SerVal: 4.13 ± 0.552
1.239SerTrp: 1.239 ± 0.271
2.643SerTyr: 2.643 ± 0.458
0.0SerXaa: 0.0 ± 0.0
Thr
6.938ThrAla: 6.938 ± 0.765
0.33ThrCys: 0.33 ± 0.154
4.873ThrAsp: 4.873 ± 0.585
2.643ThrGlu: 2.643 ± 0.38
2.726ThrPhe: 2.726 ± 0.508
3.882ThrGly: 3.882 ± 0.516
1.404ThrHis: 1.404 ± 0.428
4.378ThrIle: 4.378 ± 0.565
3.304ThrLys: 3.304 ± 0.501
5.534ThrLeu: 5.534 ± 0.695
1.652ThrMet: 1.652 ± 0.325
2.808ThrAsn: 2.808 ± 0.487
2.891ThrPro: 2.891 ± 0.574
2.561ThrGln: 2.561 ± 0.501
2.313ThrArg: 2.313 ± 0.409
4.625ThrSer: 4.625 ± 0.646
4.791ThrThr: 4.791 ± 0.524
5.534ThrVal: 5.534 ± 0.69
0.743ThrTrp: 0.743 ± 0.24
2.726ThrTyr: 2.726 ± 0.571
0.0ThrXaa: 0.0 ± 0.0
Val
6.856ValAla: 6.856 ± 0.749
0.33ValCys: 0.33 ± 0.15
4.212ValAsp: 4.212 ± 0.554
3.717ValGlu: 3.717 ± 0.635
1.9ValPhe: 1.9 ± 0.384
4.378ValGly: 4.378 ± 0.656
0.578ValHis: 0.578 ± 0.212
4.212ValIle: 4.212 ± 0.634
4.295ValLys: 4.295 ± 0.734
4.791ValLeu: 4.791 ± 0.646
1.982ValMet: 1.982 ± 0.381
3.469ValAsn: 3.469 ± 0.509
1.982ValPro: 1.982 ± 0.433
2.395ValGln: 2.395 ± 0.628
2.478ValArg: 2.478 ± 0.466
4.956ValSer: 4.956 ± 0.812
4.543ValThr: 4.543 ± 0.529
3.139ValVal: 3.139 ± 0.422
1.239ValTrp: 1.239 ± 0.402
2.065ValTyr: 2.065 ± 0.569
0.0ValXaa: 0.0 ± 0.0
Trp
0.661TrpAla: 0.661 ± 0.223
0.248TrpCys: 0.248 ± 0.154
0.909TrpAsp: 0.909 ± 0.251
0.578TrpGlu: 0.578 ± 0.245
0.661TrpPhe: 0.661 ± 0.245
1.156TrpGly: 1.156 ± 0.384
0.413TrpHis: 0.413 ± 0.21
1.404TrpIle: 1.404 ± 0.277
0.826TrpLys: 0.826 ± 0.273
1.569TrpLeu: 1.569 ± 0.376
0.165TrpMet: 0.165 ± 0.13
1.817TrpAsn: 1.817 ± 0.839
0.083TrpPro: 0.083 ± 0.082
1.074TrpGln: 1.074 ± 0.315
0.661TrpArg: 0.661 ± 0.264
1.404TrpSer: 1.404 ± 0.612
1.156TrpThr: 1.156 ± 0.304
0.826TrpVal: 0.826 ± 0.268
0.248TrpTrp: 0.248 ± 0.138
0.578TrpTyr: 0.578 ± 0.238
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.304TyrAla: 3.304 ± 0.376
0.413TyrCys: 0.413 ± 0.197
3.139TyrAsp: 3.139 ± 0.438
1.817TyrGlu: 1.817 ± 0.453
1.982TyrPhe: 1.982 ± 0.312
2.148TyrGly: 2.148 ± 0.468
0.743TyrHis: 0.743 ± 0.251
2.23TyrIle: 2.23 ± 0.51
2.891TyrLys: 2.891 ± 0.58
2.973TyrLeu: 2.973 ± 0.596
0.743TyrMet: 0.743 ± 0.244
2.313TyrAsn: 2.313 ± 0.381
1.735TyrPro: 1.735 ± 0.443
1.817TyrGln: 1.817 ± 0.425
1.9TyrArg: 1.9 ± 0.372
2.313TyrSer: 2.313 ± 0.527
2.148TyrThr: 2.148 ± 0.47
1.982TyrVal: 1.982 ± 0.408
0.826TyrTrp: 0.826 ± 0.287
1.817TyrTyr: 1.817 ± 0.502
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12108 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski