Chandiru virus
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2W3Q3|F2W3Q3_9VIRU Nonstructural protein OS=Chandiru virus OX=629725 PE=4 SV=1
MM1 pKa = 7.93 SNMLNNVFDD10 pKa = 3.97 IPHH13 pKa = 6.19 ITRR16 pKa = 11.84 SATDD20 pKa = 3.22 RR21 pKa = 11.84 SKK23 pKa = 11.57 VYY25 pKa = 10.74 VEE27 pKa = 3.76 YY28 pKa = 10.58 SAYY31 pKa = 9.99 NGYY34 pKa = 9.32 WGTPICRR41 pKa = 11.84 YY42 pKa = 9.78 KK43 pKa = 10.84 GAEE46 pKa = 4.29 FPCSSFTVSDD56 pKa = 3.33 KK57 pKa = 10.84 TSFRR61 pKa = 11.84 LRR63 pKa = 11.84 SFIDD67 pKa = 3.03 KK68 pKa = 10.62 GQLPRR73 pKa = 11.84 SWGKK77 pKa = 9.57 PFLQVKK83 pKa = 9.59 GKK85 pKa = 10.26 SPSFFDD91 pKa = 3.31 TTIEE95 pKa = 4.51 KK96 pKa = 9.97 ISHH99 pKa = 6.52 LNLEE103 pKa = 4.68 RR104 pKa = 11.84 QLKK107 pKa = 8.9 WSEE110 pKa = 4.12 PNLKK114 pKa = 9.92 EE115 pKa = 4.11 ALSWPLGVPTLAFFKK130 pKa = 10.8 LSLVEE135 pKa = 4.31 SYY137 pKa = 10.81 EE138 pKa = 4.2 FNWEE142 pKa = 4.1 EE143 pKa = 4.05 KK144 pKa = 10.17 CFLMTLIMRR153 pKa = 11.84 SGDD156 pKa = 3.18 GAQIDD161 pKa = 3.99 DD162 pKa = 4.52 SLVNLYY168 pKa = 10.46 KK169 pKa = 10.75 KK170 pKa = 10.69 LIRR173 pKa = 11.84 EE174 pKa = 4.05 LGEE177 pKa = 4.03 RR178 pKa = 11.84 DD179 pKa = 3.46 IPCLNFTGQNIEE191 pKa = 4.0 KK192 pKa = 9.61 EE193 pKa = 3.86 IAYY196 pKa = 9.34 VQVLRR201 pKa = 11.84 MLTALPYY208 pKa = 10.31 DD209 pKa = 4.01 YY210 pKa = 11.08 YY211 pKa = 11.1 EE212 pKa = 5.13 SDD214 pKa = 3.74 FHH216 pKa = 8.58 SSLYY220 pKa = 10.77 DD221 pKa = 3.52 YY222 pKa = 11.14 VKK224 pKa = 10.47 EE225 pKa = 3.83 LSEE228 pKa = 4.7 VITPHH233 pKa = 7.08 LLGNRR238 pKa = 11.84 KK239 pKa = 6.41 WTPLHH244 pKa = 5.75 TMSPRR249 pKa = 11.84 YY250 pKa = 8.23 TRR252 pKa = 11.84 IADD255 pKa = 3.47 SWDD258 pKa = 3.26 SDD260 pKa = 3.74 LDD262 pKa = 3.8 SEE264 pKa = 5.02 EE265 pKa = 5.14 DD266 pKa = 3.63 MEE268 pKa = 6.22 IDD270 pKa = 3.88 GPAARR275 pKa = 11.84 WGEE278 pKa = 4.33 GNN280 pKa = 3.61
Molecular weight: 32.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.191
IPC2_protein 5.296
IPC_protein 5.245
Toseland 5.245
ProMoST 5.41
Dawson 5.27
Bjellqvist 5.385
Wikipedia 5.156
Rodwell 5.194
Grimsley 5.194
Solomon 5.27
Lehninger 5.232
Nozaki 5.41
DTASelect 5.563
Thurlkill 5.283
EMBOSS 5.232
Sillero 5.486
Patrickios 3.973
IPC_peptide 5.283
IPC2_peptide 5.486
IPC2.peptide.svr19 5.481
Protein with the highest isoelectric point:
>tr|F2W3Q3|F2W3Q3_9VIRU Nonstructural protein OS=Chandiru virus OX=629725 PE=4 SV=1
MM1 pKa = 8.05 SYY3 pKa = 10.78 EE4 pKa = 4.0 KK5 pKa = 10.71 LAVDD9 pKa = 4.1 IAGHH13 pKa = 6.45 EE14 pKa = 4.11 IDD16 pKa = 5.31 ADD18 pKa = 4.22 TIKK21 pKa = 10.88 AWVQAFAYY29 pKa = 10.12 QGFDD33 pKa = 2.93 AKK35 pKa = 10.46 RR36 pKa = 11.84 VMEE39 pKa = 4.34 LLVEE43 pKa = 4.34 RR44 pKa = 11.84 GGDD47 pKa = 3.5 DD48 pKa = 2.96 WVEE51 pKa = 4.08 DD52 pKa = 3.91 AKK54 pKa = 11.56 QMIILCLTRR63 pKa = 11.84 GNKK66 pKa = 7.9 PSKK69 pKa = 10.39 MMVKK73 pKa = 9.95 MSEE76 pKa = 3.84 KK77 pKa = 10.19 GKK79 pKa = 10.72 KK80 pKa = 8.77 IVQALVKK87 pKa = 10.1 RR88 pKa = 11.84 YY89 pKa = 8.3 SLKK92 pKa = 10.6 EE93 pKa = 4.01 GNPSRR98 pKa = 11.84 DD99 pKa = 3.73 DD100 pKa = 3.48 LTLSRR105 pKa = 11.84 VTAALAGYY113 pKa = 6.71 TCQATEE119 pKa = 4.08 YY120 pKa = 10.23 VEE122 pKa = 4.87 EE123 pKa = 4.16 FLPVTGKK130 pKa = 10.35 NMDD133 pKa = 4.67 DD134 pKa = 3.44 LSKK137 pKa = 10.8 NYY139 pKa = 8.62 PRR141 pKa = 11.84 AMMHH145 pKa = 6.7 PSFAGLIDD153 pKa = 4.3 PKK155 pKa = 10.81 LPPDD159 pKa = 3.93 VLSTICDD166 pKa = 3.36 AFSLFMVQFSRR177 pKa = 11.84 TINPRR182 pKa = 11.84 NRR184 pKa = 11.84 GLSVSEE190 pKa = 4.14 VASTFDD196 pKa = 3.54 RR197 pKa = 11.84 PINAAMNSSFISGEE211 pKa = 3.72 QRR213 pKa = 11.84 KK214 pKa = 9.36 SFLRR218 pKa = 11.84 NLGILDD224 pKa = 4.11 EE225 pKa = 4.86 NMQPSNPVKK234 pKa = 10.34 AAAKK238 pKa = 9.76 VFRR241 pKa = 11.84 GLKK244 pKa = 9.69
Molecular weight: 27.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.021
IPC2_protein 8.053
IPC_protein 8.039
Toseland 8.521
ProMoST 8.697
Dawson 8.887
Bjellqvist 8.873
Wikipedia 8.916
Rodwell 9.019
Grimsley 8.448
Solomon 9.077
Lehninger 9.063
Nozaki 9.063
DTASelect 8.726
Thurlkill 8.858
EMBOSS 9.077
Sillero 9.077
Patrickios 4.902
IPC_peptide 9.063
IPC2_peptide 7.541
IPC2.peptide.svr19 7.631
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3932
244
2088
983.0
111.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.705 ± 0.556
2.721 ± 0.762
5.722 ± 0.606
6.943 ± 0.268
4.73 ± 0.202
5.977 ± 0.421
2.314 ± 0.275
7.35 ± 0.586
7.248 ± 0.288
8.85 ± 0.208
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.077 ± 0.321
4.171 ± 0.399
3.662 ± 0.379
3.077 ± 0.245
4.959 ± 0.162
9.613 ± 0.255
4.807 ± 0.365
5.671 ± 0.191
1.348 ± 0.18
3.052 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here