Amino acid dipepetide frequency for Roseobacter phage CRP-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.552AlaAla: 9.552 ± 1.58
0.356AlaCys: 0.356 ± 0.126
5.518AlaAsp: 5.518 ± 0.664
6.348AlaGlu: 6.348 ± 0.696
2.551AlaPhe: 2.551 ± 0.407
7.772AlaGly: 7.772 ± 0.768
1.009AlaHis: 1.009 ± 0.32
3.975AlaIle: 3.975 ± 0.467
6.526AlaLys: 6.526 ± 0.809
6.704AlaLeu: 6.704 ± 0.93
3.738AlaMet: 3.738 ± 0.546
5.933AlaAsn: 5.933 ± 0.692
3.085AlaPro: 3.085 ± 0.428
3.797AlaGln: 3.797 ± 1.012
4.984AlaArg: 4.984 ± 0.737
5.992AlaSer: 5.992 ± 0.707
7.001AlaThr: 7.001 ± 1.273
4.568AlaVal: 4.568 ± 0.55
1.543AlaTrp: 1.543 ± 0.37
2.67AlaTyr: 2.67 ± 0.384
0.0AlaXaa: 0.0 ± 0.0
Cys
0.415CysAla: 0.415 ± 0.173
0.0CysCys: 0.0 ± 0.0
0.534CysAsp: 0.534 ± 0.187
0.356CysGlu: 0.356 ± 0.172
0.297CysPhe: 0.297 ± 0.139
0.593CysGly: 0.593 ± 0.212
0.178CysHis: 0.178 ± 0.102
0.297CysIle: 0.297 ± 0.131
0.178CysLys: 0.178 ± 0.103
0.415CysLeu: 0.415 ± 0.173
0.237CysMet: 0.237 ± 0.115
0.475CysAsn: 0.475 ± 0.2
0.178CysPro: 0.178 ± 0.099
0.297CysGln: 0.297 ± 0.138
0.356CysArg: 0.356 ± 0.141
0.415CysSer: 0.415 ± 0.172
0.534CysThr: 0.534 ± 0.246
0.356CysVal: 0.356 ± 0.138
0.059CysTrp: 0.059 ± 0.069
0.119CysTyr: 0.119 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
5.696AspAla: 5.696 ± 0.607
0.237AspCys: 0.237 ± 0.106
4.153AspAsp: 4.153 ± 0.572
4.212AspGlu: 4.212 ± 0.642
2.611AspPhe: 2.611 ± 0.437
4.865AspGly: 4.865 ± 0.835
0.593AspHis: 0.593 ± 0.198
4.806AspIle: 4.806 ± 0.595
3.026AspLys: 3.026 ± 0.622
5.043AspLeu: 5.043 ± 0.457
2.492AspMet: 2.492 ± 0.391
3.322AspAsn: 3.322 ± 0.6
3.026AspPro: 3.026 ± 0.452
2.255AspGln: 2.255 ± 0.477
2.611AspArg: 2.611 ± 0.471
3.085AspSer: 3.085 ± 0.491
4.984AspThr: 4.984 ± 0.511
4.153AspVal: 4.153 ± 0.587
0.831AspTrp: 0.831 ± 0.206
2.255AspTyr: 2.255 ± 0.326
0.0AspXaa: 0.0 ± 0.0
Glu
6.645GluAla: 6.645 ± 0.816
0.475GluCys: 0.475 ± 0.164
4.331GluAsp: 4.331 ± 0.648
6.348GluGlu: 6.348 ± 1.13
2.373GluPhe: 2.373 ± 0.497
4.331GluGly: 4.331 ± 0.582
1.009GluHis: 1.009 ± 0.276
3.738GluIle: 3.738 ± 0.508
2.433GluLys: 2.433 ± 0.467
5.399GluLeu: 5.399 ± 0.778
1.483GluMet: 1.483 ± 0.291
2.551GluAsn: 2.551 ± 0.311
2.195GluPro: 2.195 ± 0.429
4.331GluGln: 4.331 ± 0.619
3.738GluArg: 3.738 ± 0.446
2.67GluSer: 2.67 ± 0.39
3.322GluThr: 3.322 ± 0.495
4.212GluVal: 4.212 ± 0.551
1.483GluTrp: 1.483 ± 0.323
2.907GluTyr: 2.907 ± 0.563
0.0GluXaa: 0.0 ± 0.0
Phe
2.966PheAla: 2.966 ± 0.405
0.415PheCys: 0.415 ± 0.182
3.085PheAsp: 3.085 ± 0.416
1.483PheGlu: 1.483 ± 0.305
1.068PhePhe: 1.068 ± 0.229
2.788PheGly: 2.788 ± 0.44
0.415PheHis: 0.415 ± 0.207
1.78PheIle: 1.78 ± 0.396
2.314PheLys: 2.314 ± 0.313
2.017PheLeu: 2.017 ± 0.38
0.949PheMet: 0.949 ± 0.22
2.788PheAsn: 2.788 ± 0.411
1.127PhePro: 1.127 ± 0.272
1.187PheGln: 1.187 ± 0.333
1.543PheArg: 1.543 ± 0.336
2.373PheSer: 2.373 ± 0.412
2.67PheThr: 2.67 ± 0.453
2.551PheVal: 2.551 ± 0.388
0.119PheTrp: 0.119 ± 0.086
1.839PheTyr: 1.839 ± 0.27
0.0PheXaa: 0.0 ± 0.0
Gly
6.645GlyAla: 6.645 ± 0.815
0.415GlyCys: 0.415 ± 0.149
4.806GlyAsp: 4.806 ± 0.593
3.441GlyGlu: 3.441 ± 0.405
3.382GlyPhe: 3.382 ± 0.431
5.636GlyGly: 5.636 ± 0.768
1.246GlyHis: 1.246 ± 0.312
3.916GlyIle: 3.916 ± 0.451
4.628GlyLys: 4.628 ± 0.787
4.153GlyLeu: 4.153 ± 0.497
2.077GlyMet: 2.077 ± 0.299
4.568GlyAsn: 4.568 ± 1.088
1.661GlyPro: 1.661 ± 0.288
3.441GlyGln: 3.441 ± 0.697
2.907GlyArg: 2.907 ± 0.515
5.577GlySer: 5.577 ± 1.005
7.535GlyThr: 7.535 ± 1.765
4.806GlyVal: 4.806 ± 0.612
0.653GlyTrp: 0.653 ± 0.189
3.678GlyTyr: 3.678 ± 0.769
0.0GlyXaa: 0.0 ± 0.0
His
1.483HisAla: 1.483 ± 0.318
0.119HisCys: 0.119 ± 0.087
1.187HisAsp: 1.187 ± 0.269
0.831HisGlu: 0.831 ± 0.288
0.237HisPhe: 0.237 ± 0.125
0.949HisGly: 0.949 ± 0.221
0.237HisHis: 0.237 ± 0.114
0.534HisIle: 0.534 ± 0.179
0.89HisLys: 0.89 ± 0.206
1.187HisLeu: 1.187 ± 0.296
0.593HisMet: 0.593 ± 0.216
1.127HisAsn: 1.127 ± 0.245
1.009HisPro: 1.009 ± 0.261
0.415HisGln: 0.415 ± 0.15
0.771HisArg: 0.771 ± 0.223
1.068HisSer: 1.068 ± 0.275
0.949HisThr: 0.949 ± 0.239
1.424HisVal: 1.424 ± 0.291
0.297HisTrp: 0.297 ± 0.137
0.297HisTyr: 0.297 ± 0.163
0.0HisXaa: 0.0 ± 0.0
Ile
4.865IleAla: 4.865 ± 0.546
0.178IleCys: 0.178 ± 0.106
3.856IleAsp: 3.856 ± 0.646
3.204IleGlu: 3.204 ± 0.514
1.483IlePhe: 1.483 ± 0.298
2.966IleGly: 2.966 ± 0.6
0.534IleHis: 0.534 ± 0.214
2.373IleIle: 2.373 ± 0.363
3.619IleLys: 3.619 ± 0.593
2.195IleLeu: 2.195 ± 0.397
1.365IleMet: 1.365 ± 0.236
2.788IleAsn: 2.788 ± 0.391
2.433IlePro: 2.433 ± 0.512
2.136IleGln: 2.136 ± 0.405
3.085IleArg: 3.085 ± 0.513
2.551IleSer: 2.551 ± 0.448
2.966IleThr: 2.966 ± 0.526
3.263IleVal: 3.263 ± 0.465
0.356IleTrp: 0.356 ± 0.119
1.365IleTyr: 1.365 ± 0.267
0.0IleXaa: 0.0 ± 0.0
Lys
6.052LysAla: 6.052 ± 0.658
0.356LysCys: 0.356 ± 0.156
3.204LysAsp: 3.204 ± 0.502
4.094LysGlu: 4.094 ± 0.658
1.721LysPhe: 1.721 ± 0.277
2.907LysGly: 2.907 ± 0.518
1.246LysHis: 1.246 ± 0.279
2.611LysIle: 2.611 ± 0.37
4.212LysLys: 4.212 ± 0.789
4.568LysLeu: 4.568 ± 0.575
1.958LysMet: 1.958 ± 0.349
2.433LysAsn: 2.433 ± 0.373
2.551LysPro: 2.551 ± 0.429
3.322LysGln: 3.322 ± 0.596
2.433LysArg: 2.433 ± 0.495
3.204LysSer: 3.204 ± 0.461
3.5LysThr: 3.5 ± 0.564
3.797LysVal: 3.797 ± 0.539
1.009LysTrp: 1.009 ± 0.309
1.839LysTyr: 1.839 ± 0.304
0.0LysXaa: 0.0 ± 0.0
Leu
6.645LeuAla: 6.645 ± 0.711
0.356LeuCys: 0.356 ± 0.182
4.865LeuAsp: 4.865 ± 0.699
4.39LeuGlu: 4.39 ± 0.526
2.255LeuPhe: 2.255 ± 0.36
5.399LeuGly: 5.399 ± 0.728
1.246LeuHis: 1.246 ± 0.328
2.729LeuIle: 2.729 ± 0.478
3.678LeuLys: 3.678 ± 0.51
5.102LeuLeu: 5.102 ± 0.63
1.78LeuMet: 1.78 ± 0.392
4.212LeuAsn: 4.212 ± 0.509
3.144LeuPro: 3.144 ± 0.488
2.67LeuGln: 2.67 ± 0.397
4.034LeuArg: 4.034 ± 0.643
5.696LeuSer: 5.696 ± 0.464
5.399LeuThr: 5.399 ± 0.668
3.263LeuVal: 3.263 ± 0.386
0.831LeuTrp: 0.831 ± 0.247
2.729LeuTyr: 2.729 ± 0.383
0.0LeuXaa: 0.0 ± 0.0
Met
2.848MetAla: 2.848 ± 0.521
0.178MetCys: 0.178 ± 0.102
1.661MetAsp: 1.661 ± 0.314
2.551MetGlu: 2.551 ± 0.5
0.831MetPhe: 0.831 ± 0.297
2.551MetGly: 2.551 ± 0.336
0.534MetHis: 0.534 ± 0.212
1.009MetIle: 1.009 ± 0.276
1.721MetLys: 1.721 ± 0.334
2.255MetLeu: 2.255 ± 0.402
0.831MetMet: 0.831 ± 0.225
1.602MetAsn: 1.602 ± 0.351
0.949MetPro: 0.949 ± 0.294
1.602MetGln: 1.602 ± 0.381
2.017MetArg: 2.017 ± 0.306
2.373MetSer: 2.373 ± 0.41
1.958MetThr: 1.958 ± 0.401
1.721MetVal: 1.721 ± 0.331
0.356MetTrp: 0.356 ± 0.168
0.949MetTyr: 0.949 ± 0.231
0.0MetXaa: 0.0 ± 0.0
Asn
5.102AsnAla: 5.102 ± 0.841
0.593AsnCys: 0.593 ± 0.236
2.788AsnAsp: 2.788 ± 0.393
2.848AsnGlu: 2.848 ± 0.33
1.839AsnPhe: 1.839 ± 0.283
4.806AsnGly: 4.806 ± 0.838
1.009AsnHis: 1.009 ± 0.26
3.263AsnIle: 3.263 ± 0.51
2.67AsnLys: 2.67 ± 0.502
4.034AsnLeu: 4.034 ± 0.487
1.899AsnMet: 1.899 ± 0.388
3.5AsnAsn: 3.5 ± 0.666
2.551AsnPro: 2.551 ± 0.44
1.958AsnGln: 1.958 ± 0.371
1.78AsnArg: 1.78 ± 0.29
2.966AsnSer: 2.966 ± 0.574
4.687AsnThr: 4.687 ± 1.562
3.797AsnVal: 3.797 ± 0.514
0.712AsnTrp: 0.712 ± 0.216
1.78AsnTyr: 1.78 ± 0.241
0.0AsnXaa: 0.0 ± 0.0
Pro
3.263ProAla: 3.263 ± 0.5
0.237ProCys: 0.237 ± 0.152
2.67ProAsp: 2.67 ± 0.436
3.441ProGlu: 3.441 ± 0.574
1.839ProPhe: 1.839 ± 0.338
0.771ProGly: 0.771 ± 0.211
0.653ProHis: 0.653 ± 0.15
1.483ProIle: 1.483 ± 0.281
2.195ProLys: 2.195 ± 0.469
1.78ProLeu: 1.78 ± 0.335
1.009ProMet: 1.009 ± 0.269
1.78ProAsn: 1.78 ± 0.29
1.068ProPro: 1.068 ± 0.364
1.78ProGln: 1.78 ± 0.682
1.246ProArg: 1.246 ± 0.239
2.966ProSer: 2.966 ± 0.463
3.738ProThr: 3.738 ± 0.554
2.848ProVal: 2.848 ± 0.389
0.415ProTrp: 0.415 ± 0.176
1.543ProTyr: 1.543 ± 0.401
0.0ProXaa: 0.0 ± 0.0
Gln
4.628GlnAla: 4.628 ± 1.082
0.178GlnCys: 0.178 ± 0.099
1.839GlnAsp: 1.839 ± 0.344
3.204GlnGlu: 3.204 ± 0.627
2.136GlnPhe: 2.136 ± 0.467
2.966GlnGly: 2.966 ± 0.44
0.653GlnHis: 0.653 ± 0.217
1.958GlnIle: 1.958 ± 0.355
2.017GlnLys: 2.017 ± 0.411
3.738GlnLeu: 3.738 ± 0.561
1.78GlnMet: 1.78 ± 0.443
2.077GlnAsn: 2.077 ± 0.44
1.721GlnPro: 1.721 ± 0.519
3.441GlnGln: 3.441 ± 1.168
2.255GlnArg: 2.255 ± 0.421
2.551GlnSer: 2.551 ± 0.519
3.263GlnThr: 3.263 ± 0.698
2.67GlnVal: 2.67 ± 0.57
0.653GlnTrp: 0.653 ± 0.194
1.483GlnTyr: 1.483 ± 0.332
0.0GlnXaa: 0.0 ± 0.0
Arg
3.144ArgAla: 3.144 ± 0.511
0.178ArgCys: 0.178 ± 0.112
2.966ArgAsp: 2.966 ± 0.439
4.331ArgGlu: 4.331 ± 0.594
2.077ArgPhe: 2.077 ± 0.359
2.729ArgGly: 2.729 ± 0.446
0.534ArgHis: 0.534 ± 0.239
2.017ArgIle: 2.017 ± 0.295
3.738ArgLys: 3.738 ± 0.608
3.619ArgLeu: 3.619 ± 0.559
1.78ArgMet: 1.78 ± 0.296
2.67ArgAsn: 2.67 ± 0.323
1.483ArgPro: 1.483 ± 0.326
2.551ArgGln: 2.551 ± 0.358
3.204ArgArg: 3.204 ± 0.448
2.848ArgSer: 2.848 ± 0.524
2.551ArgThr: 2.551 ± 0.463
2.848ArgVal: 2.848 ± 0.47
0.712ArgTrp: 0.712 ± 0.167
2.195ArgTyr: 2.195 ± 0.371
0.0ArgXaa: 0.0 ± 0.0
Ser
5.636SerAla: 5.636 ± 0.821
0.415SerCys: 0.415 ± 0.151
3.975SerAsp: 3.975 ± 0.397
3.441SerGlu: 3.441 ± 0.613
2.67SerPhe: 2.67 ± 0.375
8.306SerGly: 8.306 ± 1.039
1.127SerHis: 1.127 ± 0.257
2.907SerIle: 2.907 ± 0.711
3.144SerLys: 3.144 ± 0.407
4.806SerLeu: 4.806 ± 0.583
1.305SerMet: 1.305 ± 0.32
3.382SerAsn: 3.382 ± 0.832
1.958SerPro: 1.958 ± 0.4
2.492SerGln: 2.492 ± 0.393
2.611SerArg: 2.611 ± 0.387
5.399SerSer: 5.399 ± 1.145
4.034SerThr: 4.034 ± 0.686
3.856SerVal: 3.856 ± 0.529
0.712SerTrp: 0.712 ± 0.247
2.492SerTyr: 2.492 ± 0.513
0.0SerXaa: 0.0 ± 0.0
Thr
7.713ThrAla: 7.713 ± 1.201
0.653ThrCys: 0.653 ± 0.182
4.687ThrAsp: 4.687 ± 0.548
4.153ThrGlu: 4.153 ± 0.552
2.729ThrPhe: 2.729 ± 0.326
7.357ThrGly: 7.357 ± 1.202
1.009ThrHis: 1.009 ± 0.262
2.788ThrIle: 2.788 ± 0.515
3.678ThrLys: 3.678 ± 0.386
5.221ThrLeu: 5.221 ± 0.744
2.195ThrMet: 2.195 ± 0.325
3.619ThrAsn: 3.619 ± 0.881
2.907ThrPro: 2.907 ± 0.527
2.729ThrGln: 2.729 ± 0.491
1.899ThrArg: 1.899 ± 0.349
5.399ThrSer: 5.399 ± 1.181
7.535ThrThr: 7.535 ± 1.716
5.458ThrVal: 5.458 ± 0.689
0.712ThrTrp: 0.712 ± 0.269
2.848ThrTyr: 2.848 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
6.23ValAla: 6.23 ± 0.49
0.475ValCys: 0.475 ± 0.166
5.221ValAsp: 5.221 ± 0.747
4.094ValGlu: 4.094 ± 0.542
1.721ValPhe: 1.721 ± 0.33
4.806ValGly: 4.806 ± 0.615
1.187ValHis: 1.187 ± 0.327
2.907ValIle: 2.907 ± 0.459
3.797ValLys: 3.797 ± 0.436
4.272ValLeu: 4.272 ± 0.537
1.483ValMet: 1.483 ± 0.311
3.026ValAsn: 3.026 ± 0.449
2.017ValPro: 2.017 ± 0.369
2.373ValGln: 2.373 ± 0.486
3.263ValArg: 3.263 ± 0.695
4.45ValSer: 4.45 ± 0.556
5.34ValThr: 5.34 ± 0.991
4.687ValVal: 4.687 ± 0.506
0.712ValTrp: 0.712 ± 0.18
1.899ValTyr: 1.899 ± 0.378
0.0ValXaa: 0.0 ± 0.0
Trp
1.246TrpAla: 1.246 ± 0.299
0.237TrpCys: 0.237 ± 0.12
0.653TrpAsp: 0.653 ± 0.149
0.653TrpGlu: 0.653 ± 0.207
0.534TrpPhe: 0.534 ± 0.182
0.415TrpGly: 0.415 ± 0.151
0.475TrpHis: 0.475 ± 0.161
0.415TrpIle: 0.415 ± 0.142
0.831TrpLys: 0.831 ± 0.192
0.949TrpLeu: 0.949 ± 0.3
0.297TrpMet: 0.297 ± 0.166
0.475TrpAsn: 0.475 ± 0.163
0.534TrpPro: 0.534 ± 0.179
0.415TrpGln: 0.415 ± 0.139
1.127TrpArg: 1.127 ± 0.297
0.593TrpSer: 0.593 ± 0.182
0.771TrpThr: 0.771 ± 0.209
1.365TrpVal: 1.365 ± 0.28
0.237TrpTrp: 0.237 ± 0.117
0.653TrpTyr: 0.653 ± 0.221
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.848TyrAla: 2.848 ± 0.348
0.237TyrCys: 0.237 ± 0.141
2.255TyrAsp: 2.255 ± 0.259
2.551TyrGlu: 2.551 ± 0.402
1.127TyrPhe: 1.127 ± 0.251
2.255TyrGly: 2.255 ± 0.411
0.712TyrHis: 0.712 ± 0.202
2.077TyrIle: 2.077 ± 0.381
1.78TyrLys: 1.78 ± 0.335
2.966TyrLeu: 2.966 ± 0.396
1.187TyrMet: 1.187 ± 0.284
2.255TyrAsn: 2.255 ± 0.539
1.127TyrPro: 1.127 ± 0.307
2.017TyrGln: 2.017 ± 0.45
2.314TyrArg: 2.314 ± 0.47
2.492TyrSer: 2.492 ± 0.385
2.551TyrThr: 2.551 ± 0.417
2.314TyrVal: 2.314 ± 0.48
0.475TyrTrp: 0.475 ± 0.175
1.068TyrTyr: 1.068 ± 0.245
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (16856 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski