Babesia microti (strain RI)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Piroplasmida; Babesiidae; Babesia; Babesia microti

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3595 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6LWT8|A0A1N6LWT8_BABMR Uncharacterized protein OS=Babesia microti (strain RI) OX=1133968 GN=BMR1_01G01895 PE=4 SV=1
MM1 pKa = 7.73SDD3 pKa = 4.64ANDD6 pKa = 3.57EE7 pKa = 4.34CDD9 pKa = 3.59VLAEE13 pKa = 4.6IILVGTSLGSVRR25 pKa = 11.84KK26 pKa = 9.79QYY28 pKa = 10.99FDD30 pKa = 3.62SKK32 pKa = 10.93RR33 pKa = 11.84LANLLDD39 pKa = 4.19CKK41 pKa = 10.89AVIYY45 pKa = 9.47YY46 pKa = 10.65LIDD49 pKa = 4.18ANLDD53 pKa = 3.39SSVAKK58 pKa = 10.22GLKK61 pKa = 9.93DD62 pKa = 3.49SEE64 pKa = 5.52LIKK67 pKa = 10.05EE68 pKa = 4.27WKK70 pKa = 9.79KK71 pKa = 10.49EE72 pKa = 4.09GVFKK76 pKa = 10.89LYY78 pKa = 10.29PNSTNEE84 pKa = 3.83IILPQLIVDD93 pKa = 5.13GIGIGDD99 pKa = 3.86YY100 pKa = 11.49EE101 pKa = 4.3MVQDD105 pKa = 5.61FEE107 pKa = 6.4DD108 pKa = 5.14DD109 pKa = 3.73GDD111 pKa = 4.11LEE113 pKa = 4.84YY114 pKa = 10.97IMTRR118 pKa = 11.84LMCPACFKK126 pKa = 11.08DD127 pKa = 3.58KK128 pKa = 10.14TAEE131 pKa = 3.91QVFCNNCGVTLTNLIPQEE149 pKa = 4.42YY150 pKa = 9.52IDD152 pKa = 5.47DD153 pKa = 3.87NTIQRR158 pKa = 11.84IYY160 pKa = 10.06MGDD163 pKa = 3.15IYY165 pKa = 10.9EE166 pKa = 4.29EE167 pKa = 4.89EE168 pKa = 4.46YY169 pKa = 10.72TDD171 pKa = 4.87SEE173 pKa = 4.49

Molecular weight: 19.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7IPZ2|I7IPZ2_BABMR Uncharacterized protein OS=Babesia microti (strain RI) OX=1133968 GN=BMR1_02g00875 PE=4 SV=1
MM1 pKa = 7.76GSPDD5 pKa = 3.36EE6 pKa = 4.23NGRR9 pKa = 11.84VYY11 pKa = 10.94VGNLPSEE18 pKa = 4.34CDD20 pKa = 3.03QRR22 pKa = 11.84EE23 pKa = 3.99IEE25 pKa = 4.23EE26 pKa = 4.13EE27 pKa = 3.92FEE29 pKa = 4.1KK30 pKa = 10.75FGKK33 pKa = 9.77IKK35 pKa = 10.64RR36 pKa = 11.84CDD38 pKa = 3.46VKK40 pKa = 10.89RR41 pKa = 11.84GANGSSFAFVEE52 pKa = 4.75FEE54 pKa = 4.55DD55 pKa = 4.72PRR57 pKa = 11.84DD58 pKa = 3.52AKK60 pKa = 10.98DD61 pKa = 3.74AIKK64 pKa = 10.7EE65 pKa = 3.77KK66 pKa = 10.68DD67 pKa = 3.28GYY69 pKa = 8.55EE70 pKa = 4.19FKK72 pKa = 10.87GSRR75 pKa = 11.84LRR77 pKa = 11.84VEE79 pKa = 4.84VPFSDD84 pKa = 2.59RR85 pKa = 11.84GYY87 pKa = 9.86SRR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84PTPRR95 pKa = 11.84RR96 pKa = 11.84GHH98 pKa = 4.88YY99 pKa = 8.22TVEE102 pKa = 4.21VLGLPPSGSWQDD114 pKa = 4.13LKK116 pKa = 11.74DD117 pKa = 3.54HH118 pKa = 6.3MRR120 pKa = 11.84DD121 pKa = 3.3AGEE124 pKa = 4.73CGHH127 pKa = 7.15ADD129 pKa = 3.49VFRR132 pKa = 11.84GGVGEE137 pKa = 3.97VSFFSRR143 pKa = 11.84RR144 pKa = 11.84DD145 pKa = 2.89MDD147 pKa = 3.85AAIEE151 pKa = 4.14MFDD154 pKa = 3.04GSTFRR159 pKa = 11.84SHH161 pKa = 6.76EE162 pKa = 4.15GEE164 pKa = 3.97KK165 pKa = 10.92AKK167 pKa = 9.41ITVRR171 pKa = 11.84EE172 pKa = 3.68KK173 pKa = 9.98RR174 pKa = 11.84SRR176 pKa = 11.84YY177 pKa = 9.13RR178 pKa = 11.84NSSRR182 pKa = 11.84SGSRR186 pKa = 11.84PRR188 pKa = 11.84RR189 pKa = 11.84RR190 pKa = 11.84YY191 pKa = 9.43RR192 pKa = 11.84SRR194 pKa = 11.84TRR196 pKa = 11.84SRR198 pKa = 11.84SRR200 pKa = 11.84SYY202 pKa = 10.96RR203 pKa = 11.84SRR205 pKa = 11.84TRR207 pKa = 11.84SFSRR211 pKa = 11.84SRR213 pKa = 11.84SHH215 pKa = 6.15SRR217 pKa = 11.84TRR219 pKa = 11.84SRR221 pKa = 11.84SYY223 pKa = 10.4SRR225 pKa = 11.84RR226 pKa = 11.84RR227 pKa = 3.29

Molecular weight: 26.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3595

0

3595

1580787

31

4337

439.7

49.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.005 ± 0.042

2.125 ± 0.021

5.811 ± 0.027

5.564 ± 0.034

4.47 ± 0.025

4.926 ± 0.039

2.408 ± 0.014

8.058 ± 0.044

7.209 ± 0.046

10.01 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.206 ± 0.014

6.408 ± 0.043

3.881 ± 0.027

3.338 ± 0.02

4.392 ± 0.03

8.383 ± 0.033

5.377 ± 0.022

5.506 ± 0.026

0.956 ± 0.009

3.967 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski