Bos taurus papillomavirus 15
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5JF74|A0A0B5JF74_BPV3 E8 protein OS=Bos taurus papillomavirus 15 OX=1969700 GN=E5 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.38 GQDD5 pKa = 3.51 VVLQDD10 pKa = 4.08 LASEE14 pKa = 4.65 LEE16 pKa = 4.95 DD17 pKa = 3.08 IVSPINLDD25 pKa = 3.47 CEE27 pKa = 4.39 EE28 pKa = 4.55 EE29 pKa = 4.24 IEE31 pKa = 4.29 TEE33 pKa = 4.04 EE34 pKa = 5.12 VEE36 pKa = 4.47 VDD38 pKa = 4.11 FPNPYY43 pKa = 10.16 EE44 pKa = 4.09 ITAVCYY50 pKa = 10.43 VCEE53 pKa = 4.03 EE54 pKa = 4.21 TLRR57 pKa = 11.84 IAVVTTTTGIRR68 pKa = 11.84 DD69 pKa = 4.01 LQTLLLGSLSLLCAACSRR87 pKa = 11.84 DD88 pKa = 3.36 AFCNRR93 pKa = 11.84 RR94 pKa = 11.84 PQRR97 pKa = 11.84 NGFF100 pKa = 3.55
Molecular weight: 11.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.832
IPC2_protein 4.075
IPC_protein 3.973
Toseland 3.795
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.808
Rodwell 3.808
Grimsley 3.706
Solomon 3.91
Lehninger 3.872
Nozaki 4.05
DTASelect 4.177
Thurlkill 3.821
EMBOSS 3.821
Sillero 4.075
Patrickios 1.901
IPC_peptide 3.91
IPC2_peptide 4.062
IPC2.peptide.svr19 3.992
Protein with the highest isoelectric point:
>tr|A0A0B5JS20|A0A0B5JS20_BPV3 Minor capsid protein L2 OS=Bos taurus papillomavirus 15 OX=1969700 GN=L2 PE=3 SV=1
MM1 pKa = 8.14 DD2 pKa = 4.43 SLEE5 pKa = 4.14 ARR7 pKa = 11.84 FDD9 pKa = 3.76 ALQDD13 pKa = 3.41 QLLEE17 pKa = 4.52 IIEE20 pKa = 4.46 RR21 pKa = 11.84 DD22 pKa = 3.73 SNTLEE27 pKa = 3.85 VAIDD31 pKa = 3.46 YY32 pKa = 7.97 WSLLRR37 pKa = 11.84 KK38 pKa = 9.53 EE39 pKa = 4.07 NAFYY43 pKa = 11.11 YY44 pKa = 8.76 HH45 pKa = 6.94 ARR47 pKa = 11.84 MQGKK51 pKa = 7.27 TRR53 pKa = 11.84 LGLYY57 pKa = 7.31 TVPTARR63 pKa = 11.84 VAEE66 pKa = 4.66 HH67 pKa = 6.64 KK68 pKa = 10.61 AKK70 pKa = 10.38 EE71 pKa = 4.07 AIKK74 pKa = 10.25 ISLYY78 pKa = 10.73 LKK80 pKa = 10.21 SLQKK84 pKa = 10.58 SEE86 pKa = 4.09 FANLRR91 pKa = 11.84 WSLVDD96 pKa = 3.31 TSSEE100 pKa = 4.04 NFSARR105 pKa = 11.84 PEE107 pKa = 3.91 NTFKK111 pKa = 11.11 KK112 pKa = 9.81 KK113 pKa = 9.5 GQHH116 pKa = 3.94 VTVIYY121 pKa = 10.81 DD122 pKa = 3.41 SDD124 pKa = 4.39 SNNSMIYY131 pKa = 8.23 TAWGEE136 pKa = 3.73 IFYY139 pKa = 10.93 VDD141 pKa = 5.86 EE142 pKa = 4.36 EE143 pKa = 4.67 DD144 pKa = 3.27 TWHH147 pKa = 6.88 KK148 pKa = 10.95 AKK150 pKa = 10.83 SDD152 pKa = 3.21 VDD154 pKa = 3.77 YY155 pKa = 11.6 DD156 pKa = 4.21 GISYY160 pKa = 10.09 TDD162 pKa = 3.51 YY163 pKa = 10.6 LGEE166 pKa = 3.75 RR167 pKa = 11.84 HH168 pKa = 6.1 VYY170 pKa = 10.62 VNFHH174 pKa = 7.17 DD175 pKa = 4.41 DD176 pKa = 3.17 ARR178 pKa = 11.84 LYY180 pKa = 9.69 STLGQWEE187 pKa = 4.19 VHH189 pKa = 5.83 FEE191 pKa = 4.2 NKK193 pKa = 9.46 VLSPPVTSSIPPGPTDD209 pKa = 3.13 RR210 pKa = 11.84 RR211 pKa = 11.84 RR212 pKa = 11.84 PAEE215 pKa = 4.22 TGQHH219 pKa = 5.82 PSGLKK224 pKa = 9.45 TGLTARR230 pKa = 11.84 KK231 pKa = 9.77 SSITHH236 pKa = 5.82 SRR238 pKa = 11.84 DD239 pKa = 2.54 SRR241 pKa = 11.84 QRR243 pKa = 11.84 SRR245 pKa = 11.84 SHH247 pKa = 5.28 SRR249 pKa = 11.84 SRR251 pKa = 11.84 SRR253 pKa = 11.84 SRR255 pKa = 11.84 SPTKK259 pKa = 10.55 RR260 pKa = 11.84 PDD262 pKa = 3.0 SRR264 pKa = 11.84 GRR266 pKa = 11.84 GTEE269 pKa = 3.66 LQGPRR274 pKa = 11.84 RR275 pKa = 11.84 GRR277 pKa = 11.84 GGGGGGGDD285 pKa = 3.86 FGEE288 pKa = 4.94 PSPPSPGQVGEE299 pKa = 4.13 RR300 pKa = 11.84 HH301 pKa = 6.14 RR302 pKa = 11.84 SPSRR306 pKa = 11.84 KK307 pKa = 8.6 SASRR311 pKa = 11.84 VAQLIEE317 pKa = 4.04 EE318 pKa = 4.54 AKK320 pKa = 10.7 DD321 pKa = 3.55 PPVLLLEE328 pKa = 4.64 GGANILKK335 pKa = 9.99 CFRR338 pKa = 11.84 RR339 pKa = 11.84 RR340 pKa = 11.84 CTQTHH345 pKa = 5.17 PHH347 pKa = 7.12 RR348 pKa = 11.84 FQCMSTSWTWVSKK361 pKa = 9.55 TSTVKK366 pKa = 10.15 SRR368 pKa = 11.84 HH369 pKa = 5.14 RR370 pKa = 11.84 MLVAFTNCDD379 pKa = 3.06 QRR381 pKa = 11.84 KK382 pKa = 8.93 GFLLNVRR389 pKa = 11.84 LPKK392 pKa = 10.67 GVTAVKK398 pKa = 10.65 GCLDD402 pKa = 3.55 SLL404 pKa = 4.17
Molecular weight: 45.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.16
IPC2_protein 9.077
IPC_protein 9.077
Toseland 9.765
ProMoST 9.502
Dawson 10.014
Bjellqvist 9.706
Wikipedia 10.189
Rodwell 10.306
Grimsley 10.087
Solomon 10.043
Lehninger 9.999
Nozaki 9.838
DTASelect 9.692
Thurlkill 9.853
EMBOSS 10.189
Sillero 9.94
Patrickios 7.629
IPC_peptide 10.043
IPC2_peptide 8.39
IPC2.peptide.svr19 8.085
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2180
42
609
363.3
40.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.367 ± 0.634
1.972 ± 0.618
5.963 ± 0.368
6.468 ± 0.415
5.0 ± 0.428
6.789 ± 0.665
2.156 ± 0.32
4.817 ± 0.655
5.046 ± 0.92
8.44 ± 1.654
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.743 ± 0.302
4.817 ± 0.704
5.963 ± 1.164
4.312 ± 0.428
6.33 ± 0.751
7.936 ± 0.714
5.963 ± 0.419
6.468 ± 0.639
1.514 ± 0.312
2.936 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here