Sulfurifustis variabilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Acidiferrobacterales; Acidiferrobacteraceae; Sulfurifustis

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3857 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B4VFR7|A0A1B4VFR7_9GAMM Uncharacterized protein OS=Sulfurifustis variabilis OX=1675686 GN=SVA_3049 PE=4 SV=1
MM1 pKa = 7.28NRR3 pKa = 11.84RR4 pKa = 11.84NAANAALAFLIGILLTTTAEE24 pKa = 3.87AGFFVGAGVSNSSYY38 pKa = 11.06DD39 pKa = 3.9YY40 pKa = 11.57SDD42 pKa = 4.65LDD44 pKa = 3.37NSSGYY49 pKa = 10.61KK50 pKa = 9.77IFAGYY55 pKa = 9.12YY56 pKa = 8.1AEE58 pKa = 4.48SGVFVEE64 pKa = 4.92AATVNLGEE72 pKa = 4.29TDD74 pKa = 3.63ADD76 pKa = 3.8GAPAGFEE83 pKa = 4.18AGGLAAYY90 pKa = 9.58VGYY93 pKa = 10.31RR94 pKa = 11.84GEE96 pKa = 4.3PPSGGFGFFGKK107 pKa = 10.16IGAYY111 pKa = 10.38SFDD114 pKa = 3.32TDD116 pKa = 5.28LIVSGTTIATEE127 pKa = 4.21SSSGLAWALGISYY140 pKa = 10.49AFNANVAVRR149 pKa = 11.84GEE151 pKa = 3.98IEE153 pKa = 4.1QFVGVEE159 pKa = 4.27DD160 pKa = 4.48FATDD164 pKa = 3.38EE165 pKa = 4.78SITGGSVSIEE175 pKa = 3.63FRR177 pKa = 11.84FF178 pKa = 3.59

Molecular weight: 18.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B4V145|A0A1B4V145_9GAMM Phosphoribosylglycinamide synthetase OS=Sulfurifustis variabilis OX=1675686 GN=SVA_0383 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.46NLLLRR7 pKa = 11.84RR8 pKa = 11.84FHH10 pKa = 7.59ALRR13 pKa = 11.84TSHH16 pKa = 6.12NHH18 pKa = 4.59WLRR21 pKa = 11.84KK22 pKa = 7.57TVGLLLVVGGCFGFLPVLGYY42 pKa = 10.44WMLPLGLALLAVDD55 pKa = 4.54FPVARR60 pKa = 11.84RR61 pKa = 11.84LNRR64 pKa = 11.84RR65 pKa = 11.84LLVTWGRR72 pKa = 11.84WRR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84VTRR79 pKa = 11.84TAGPRR84 pKa = 11.84GPSVGRR90 pKa = 11.84QRR92 pKa = 11.84VTRR95 pKa = 11.84TPGIRR100 pKa = 11.84SGPP103 pKa = 3.53

Molecular weight: 11.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3857

0

3857

1182190

41

3988

306.5

33.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.305 ± 0.058

0.898 ± 0.016

5.293 ± 0.031

6.286 ± 0.043

3.474 ± 0.025

8.402 ± 0.037

2.31 ± 0.02

4.359 ± 0.027

2.928 ± 0.039

10.825 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.083 ± 0.019

2.309 ± 0.027

5.413 ± 0.03

2.892 ± 0.026

8.784 ± 0.053

4.682 ± 0.025

4.858 ± 0.036

7.852 ± 0.038

1.385 ± 0.02

2.66 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski