Exiguobacterium phage vB_EauS-123

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4B037|A0A0U4B037_9CAUD Uncharacterized protein OS=Exiguobacterium phage vB_EauS-123 OX=1775141 GN=EauS123_00049 PE=4 SV=1
MM1 pKa = 7.57TIALYY6 pKa = 10.74VFVGMIMFFVGYY18 pKa = 10.07VVGNRR23 pKa = 11.84SGYY26 pKa = 10.19SEE28 pKa = 4.13GLYY31 pKa = 10.72DD32 pKa = 4.55AGEE35 pKa = 4.22FFADD39 pKa = 4.91AISIQVKK46 pKa = 10.41RR47 pKa = 11.84ITDD50 pKa = 3.35QDD52 pKa = 3.62GSDD55 pKa = 3.71EE56 pKa = 5.1DD57 pKa = 3.67EE58 pKa = 4.48TKK60 pKa = 11.06

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4B023|A0A0U4B023_9CAUD Uncharacterized protein OS=Exiguobacterium phage vB_EauS-123 OX=1775141 GN=EauS123_00033 PE=4 SV=1
MM1 pKa = 7.75KK2 pKa = 10.58YY3 pKa = 8.76YY4 pKa = 9.55TLKK7 pKa = 10.54EE8 pKa = 3.55AAEE11 pKa = 4.4AYY13 pKa = 9.84GININTLRR21 pKa = 11.84YY22 pKa = 9.51RR23 pKa = 11.84LFMDD27 pKa = 3.53IRR29 pKa = 11.84AEE31 pKa = 4.06RR32 pKa = 11.84DD33 pKa = 3.09LHH35 pKa = 6.11GSRR38 pKa = 11.84KK39 pKa = 9.77GGKK42 pKa = 7.95NWIITDD48 pKa = 3.66EE49 pKa = 4.05YY50 pKa = 10.46MKK52 pKa = 10.7AKK54 pKa = 9.29EE55 pKa = 3.7WRR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84KK60 pKa = 9.78

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

9522

34

754

167.1

18.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.446 ± 0.447

0.525 ± 0.102

6.333 ± 0.283

7.677 ± 0.444

4.012 ± 0.268

6.375 ± 0.476

1.617 ± 0.19

6.427 ± 0.296

7.393 ± 0.414

8.118 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.204

5.114 ± 0.246

2.836 ± 0.223

4.001 ± 0.248

5.566 ± 0.514

5.692 ± 0.322

6.522 ± 0.399

6.753 ± 0.27

1.061 ± 0.129

3.844 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski