Actinomyces slackii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2571 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S4WIV9|A0A3S4WIV9_9ACTO VTC domain OS=Actinomyces slackii OX=52774 GN=NCTC11923_00503 PE=4 SV=1
MM1 pKa = 7.45RR2 pKa = 11.84TLKK5 pKa = 10.56HH6 pKa = 6.05VLTLPMIAALALSVSGCSVVQSLIGGTHH34 pKa = 5.6SVDD37 pKa = 4.06LAVGDD42 pKa = 4.93CIKK45 pKa = 11.11SLDD48 pKa = 3.81GDD50 pKa = 3.95VTGSDD55 pKa = 3.33NQVGQVQVVDD65 pKa = 3.92CSEE68 pKa = 3.69PHH70 pKa = 6.54LYY72 pKa = 9.52EE73 pKa = 4.15VYY75 pKa = 11.09AEE77 pKa = 4.35VEE79 pKa = 4.12LSGDD83 pKa = 3.91SLPSATEE90 pKa = 3.94VEE92 pKa = 4.77TEE94 pKa = 4.73AEE96 pKa = 4.23DD97 pKa = 3.54ACFGSGFEE105 pKa = 4.6EE106 pKa = 4.62YY107 pKa = 10.56VGAPVTEE114 pKa = 4.24TEE116 pKa = 4.28YY117 pKa = 10.96VATYY121 pKa = 9.68LSPSQDD127 pKa = 2.43TWDD130 pKa = 3.65AGDD133 pKa = 3.78RR134 pKa = 11.84KK135 pKa = 8.76VTCVLSTLDD144 pKa = 3.85EE145 pKa = 4.72SEE147 pKa = 4.32LTASAKK153 pKa = 10.65DD154 pKa = 3.43SAKK157 pKa = 10.87

Molecular weight: 16.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A448K989|A0A448K989_9ACTO ?-D-glucose-1-phosphatase OS=Actinomyces slackii OX=52774 GN=NCTC11923_00032 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2571

0

2571

929870

32

4486

361.7

38.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.897 ± 0.067

0.767 ± 0.011

5.688 ± 0.041

5.92 ± 0.045

2.403 ± 0.027

9.118 ± 0.044

2.08 ± 0.024

4.134 ± 0.033

1.728 ± 0.028

10.129 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.096 ± 0.02

1.717 ± 0.022

5.869 ± 0.043

3.25 ± 0.028

7.474 ± 0.052

6.191 ± 0.043

5.966 ± 0.05

8.18 ± 0.051

1.479 ± 0.02

1.915 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski