Enterobacter sp. BIGb0383

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacter; unclassified Enterobacter

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4596 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N1ITK6|A0A3N1ITK6_9ENTR Lipid-A-disaccharide synthase OS=Enterobacter sp. BIGb0383 OX=2485118 GN=lpxB PE=3 SV=1
MM1 pKa = 7.71SDD3 pKa = 4.57DD4 pKa = 3.64IAVPLQFTDD13 pKa = 3.47AAASKK18 pKa = 10.04VKK20 pKa = 10.69NLIADD25 pKa = 3.67EE26 pKa = 5.25DD27 pKa = 4.1NPNMKK32 pKa = 10.11LRR34 pKa = 11.84VYY36 pKa = 9.16ITGGGCSGFQYY47 pKa = 11.03GFTFDD52 pKa = 4.26DD53 pKa = 3.82QVNEE57 pKa = 3.77GDD59 pKa = 3.64MTIEE63 pKa = 3.9KK64 pKa = 10.25QGVGLVVDD72 pKa = 4.57PMSLQYY78 pKa = 11.16LVGGAVDD85 pKa = 3.63YY86 pKa = 11.38TEE88 pKa = 4.64GLEE91 pKa = 4.02GSRR94 pKa = 11.84FIVTNPNATSTCGCGSSFSII114 pKa = 5.02

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N1INW7|A0A3N1INW7_9ENTR Flavin-dependent dehydrogenase OS=Enterobacter sp. BIGb0383 OX=2485118 GN=EDF81_3687 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4596

0

4596

1513582

26

4431

329.3

36.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.026 ± 0.036

1.012 ± 0.013

5.324 ± 0.028

5.534 ± 0.031

3.794 ± 0.027

7.595 ± 0.04

2.157 ± 0.018

5.615 ± 0.031

3.944 ± 0.034

11.036 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.019

3.634 ± 0.032

4.453 ± 0.029

4.525 ± 0.031

5.81 ± 0.036

6.089 ± 0.033

5.463 ± 0.033

7.084 ± 0.03

1.534 ± 0.016

2.705 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski