Streptococcus satellite phage Javan205

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZI79|A0A4D5ZI79_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan205 OX=2558567 GN=JavanS205_0011 PE=4 SV=1
MM1 pKa = 7.39LVTYY5 pKa = 8.27PALFYY10 pKa = 11.35YY11 pKa = 10.56DD12 pKa = 3.96DD13 pKa = 4.12TDD15 pKa = 3.96GASAPYY21 pKa = 9.78FVTFPDD27 pKa = 4.21FEE29 pKa = 4.62YY30 pKa = 10.92SATQGEE36 pKa = 4.64DD37 pKa = 2.95MAEE40 pKa = 3.86AMAMASEE47 pKa = 4.17WLGITLADD55 pKa = 3.95YY56 pKa = 10.83IEE58 pKa = 4.56NGRR61 pKa = 11.84DD62 pKa = 3.2IPVPSPINTLSLVDD76 pKa = 4.05NNPFRR81 pKa = 11.84DD82 pKa = 3.86DD83 pKa = 3.58KK84 pKa = 11.4DD85 pKa = 3.43IEE87 pKa = 4.32LVYY90 pKa = 10.8DD91 pKa = 4.01PSKK94 pKa = 11.29SFISMVMVDD103 pKa = 3.25VAEE106 pKa = 4.14YY107 pKa = 10.49LGSQEE112 pKa = 4.15PVKK115 pKa = 10.18KK116 pKa = 9.12TLTIPRR122 pKa = 11.84WADD125 pKa = 3.11TLGRR129 pKa = 11.84DD130 pKa = 3.39LGLNFSQTLTDD141 pKa = 5.46AIADD145 pKa = 4.09KK146 pKa = 10.69KK147 pKa = 10.83LHH149 pKa = 5.85AA150 pKa = 5.36

Molecular weight: 16.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZGQ1|A0A4D5ZGQ1_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan205 OX=2558567 GN=JavanS205_0014 PE=4 SV=1
MM1 pKa = 6.55VTVKK5 pKa = 10.36RR6 pKa = 11.84LAIEE10 pKa = 5.67AITKK14 pKa = 9.36PMKK17 pKa = 10.75LKK19 pKa = 10.93GLTKK23 pKa = 10.43AVARR27 pKa = 11.84LDD29 pKa = 4.12GEE31 pKa = 4.08RR32 pKa = 11.84LDD34 pKa = 4.32INLEE38 pKa = 3.77ALFIEE43 pKa = 5.26FEE45 pKa = 4.26SQRR48 pKa = 11.84LEE50 pKa = 3.89LDD52 pKa = 3.79KK53 pKa = 11.19IAGTKK58 pKa = 9.44GGYY61 pKa = 9.59RR62 pKa = 11.84YY63 pKa = 10.0FFLCPRR69 pKa = 11.84CQKK72 pKa = 10.16RR73 pKa = 11.84CRR75 pKa = 11.84VLYY78 pKa = 10.13KK79 pKa = 10.42RR80 pKa = 11.84EE81 pKa = 3.28IAYY84 pKa = 9.4YY85 pKa = 10.69CRR87 pKa = 11.84MCQGIHH93 pKa = 5.97KK94 pKa = 8.33QTLNRR99 pKa = 11.84SKK101 pKa = 9.84TDD103 pKa = 3.25CQYY106 pKa = 11.19YY107 pKa = 9.02WEE109 pKa = 4.9LALKK113 pKa = 9.04EE114 pKa = 3.98ARR116 pKa = 11.84KK117 pKa = 9.3IDD119 pKa = 3.84PYY121 pKa = 9.97FTPKK125 pKa = 10.2RR126 pKa = 11.84GGYY129 pKa = 8.64MFDD132 pKa = 4.25GFPEE136 pKa = 4.16RR137 pKa = 11.84PKK139 pKa = 10.79YY140 pKa = 10.05MRR142 pKa = 11.84INTYY146 pKa = 9.23SKK148 pKa = 9.46HH149 pKa = 4.09YY150 pKa = 10.03RR151 pKa = 11.84RR152 pKa = 11.84FRR154 pKa = 11.84KK155 pKa = 9.77YY156 pKa = 10.2VEE158 pKa = 4.72KK159 pKa = 10.85GDD161 pKa = 4.1SLWLKK166 pKa = 10.73GLL168 pKa = 3.78

Molecular weight: 20.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2300

40

387

153.3

17.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.348 ± 0.687

0.696 ± 0.304

5.609 ± 0.691

8.522 ± 0.929

3.478 ± 0.398

4.87 ± 0.62

1.217 ± 0.357

6.435 ± 0.465

10.261 ± 0.603

10.435 ± 0.57

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.312

5.826 ± 0.607

2.739 ± 0.355

4.13 ± 0.402

4.87 ± 0.69

5.391 ± 1.01

5.652 ± 0.466

4.609 ± 0.506

1.174 ± 0.166

5.261 ± 0.394
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski