Amino acid dipepetide frequency for Microbacterium phage Rasovi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.861AlaAla: 10.861 ± 1.351
0.729AlaCys: 0.729 ± 0.276
6.56AlaAsp: 6.56 ± 0.707
7.07AlaGlu: 7.07 ± 0.851
2.989AlaPhe: 2.989 ± 0.46
9.695AlaGly: 9.695 ± 0.992
2.405AlaHis: 2.405 ± 0.37
4.665AlaIle: 4.665 ± 0.784
6.414AlaLys: 6.414 ± 0.904
8.383AlaLeu: 8.383 ± 0.672
2.989AlaMet: 2.989 ± 0.473
4.519AlaAsn: 4.519 ± 0.521
6.414AlaPro: 6.414 ± 0.806
4.519AlaGln: 4.519 ± 0.639
5.686AlaArg: 5.686 ± 0.706
5.686AlaSer: 5.686 ± 0.698
6.05AlaThr: 6.05 ± 0.858
7.216AlaVal: 7.216 ± 0.664
2.77AlaTrp: 2.77 ± 0.486
2.916AlaTyr: 2.916 ± 0.428
0.0AlaXaa: 0.0 ± 0.0
Cys
0.364CysAla: 0.364 ± 0.152
0.146CysCys: 0.146 ± 0.097
0.364CysAsp: 0.364 ± 0.155
0.146CysGlu: 0.146 ± 0.088
0.073CysPhe: 0.073 ± 0.077
0.802CysGly: 0.802 ± 0.362
0.073CysHis: 0.073 ± 0.06
0.437CysIle: 0.437 ± 0.159
0.146CysLys: 0.146 ± 0.105
0.948CysLeu: 0.948 ± 0.27
0.073CysMet: 0.073 ± 0.083
0.219CysAsn: 0.219 ± 0.125
0.364CysPro: 0.364 ± 0.173
0.073CysGln: 0.073 ± 0.066
0.292CysArg: 0.292 ± 0.13
0.219CysSer: 0.219 ± 0.13
0.437CysThr: 0.437 ± 0.237
0.437CysVal: 0.437 ± 0.17
0.073CysTrp: 0.073 ± 0.068
0.073CysTyr: 0.073 ± 0.066
0.0CysXaa: 0.0 ± 0.0
Asp
7.216AspAla: 7.216 ± 0.757
0.219AspCys: 0.219 ± 0.155
4.373AspAsp: 4.373 ± 0.574
4.592AspGlu: 4.592 ± 0.655
2.26AspPhe: 2.26 ± 0.349
5.613AspGly: 5.613 ± 0.673
0.656AspHis: 0.656 ± 0.185
2.187AspIle: 2.187 ± 0.37
1.895AspLys: 1.895 ± 0.383
5.904AspLeu: 5.904 ± 0.842
1.385AspMet: 1.385 ± 0.323
1.749AspAsn: 1.749 ± 0.378
4.957AspPro: 4.957 ± 0.67
3.134AspGln: 3.134 ± 0.643
3.572AspArg: 3.572 ± 0.496
3.353AspSer: 3.353 ± 0.406
4.519AspThr: 4.519 ± 0.528
3.426AspVal: 3.426 ± 0.52
1.02AspTrp: 1.02 ± 0.209
1.968AspTyr: 1.968 ± 0.37
0.0AspXaa: 0.0 ± 0.0
Glu
7.581GluAla: 7.581 ± 0.646
0.292GluCys: 0.292 ± 0.182
3.863GluAsp: 3.863 ± 0.644
4.155GluGlu: 4.155 ± 0.664
3.134GluPhe: 3.134 ± 0.619
5.904GluGly: 5.904 ± 0.623
1.749GluHis: 1.749 ± 0.267
2.916GluIle: 2.916 ± 0.411
2.478GluLys: 2.478 ± 0.476
5.175GluLeu: 5.175 ± 0.445
1.531GluMet: 1.531 ± 0.304
1.385GluAsn: 1.385 ± 0.289
3.353GluPro: 3.353 ± 0.525
2.405GluGln: 2.405 ± 0.356
4.009GluArg: 4.009 ± 0.547
3.28GluSer: 3.28 ± 0.558
4.738GluThr: 4.738 ± 0.605
4.884GluVal: 4.884 ± 0.601
1.895GluTrp: 1.895 ± 0.311
1.677GluTyr: 1.677 ± 0.293
0.0GluXaa: 0.0 ± 0.0
Phe
2.916PheAla: 2.916 ± 0.312
0.146PheCys: 0.146 ± 0.1
1.968PheAsp: 1.968 ± 0.319
2.26PheGlu: 2.26 ± 0.391
0.729PhePhe: 0.729 ± 0.258
3.499PheGly: 3.499 ± 0.486
0.583PheHis: 0.583 ± 0.193
1.239PheIle: 1.239 ± 0.412
1.02PheLys: 1.02 ± 0.285
2.187PheLeu: 2.187 ± 0.311
0.364PheMet: 0.364 ± 0.15
1.093PheAsn: 1.093 ± 0.353
1.166PhePro: 1.166 ± 0.335
1.166PheGln: 1.166 ± 0.32
1.968PheArg: 1.968 ± 0.461
1.677PheSer: 1.677 ± 0.333
2.333PheThr: 2.333 ± 0.485
2.114PheVal: 2.114 ± 0.599
0.583PheTrp: 0.583 ± 0.235
0.583PheTyr: 0.583 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
9.549GlyAla: 9.549 ± 1.007
0.729GlyCys: 0.729 ± 0.285
4.373GlyAsp: 4.373 ± 0.64
5.54GlyGlu: 5.54 ± 0.465
3.134GlyPhe: 3.134 ± 0.797
6.779GlyGly: 6.779 ± 0.701
1.822GlyHis: 1.822 ± 0.454
4.665GlyIle: 4.665 ± 0.673
4.519GlyLys: 4.519 ± 0.773
7.289GlyLeu: 7.289 ± 0.914
1.749GlyMet: 1.749 ± 0.433
2.77GlyAsn: 2.77 ± 0.441
4.446GlyPro: 4.446 ± 0.806
4.592GlyGln: 4.592 ± 0.58
4.884GlyArg: 4.884 ± 0.668
4.957GlySer: 4.957 ± 0.807
5.613GlyThr: 5.613 ± 0.674
5.831GlyVal: 5.831 ± 0.522
2.187GlyTrp: 2.187 ± 0.402
2.77GlyTyr: 2.77 ± 0.374
0.0GlyXaa: 0.0 ± 0.0
His
0.875HisAla: 0.875 ± 0.236
0.0HisCys: 0.0 ± 0.0
2.187HisAsp: 2.187 ± 0.517
0.875HisGlu: 0.875 ± 0.277
0.729HisPhe: 0.729 ± 0.236
1.604HisGly: 1.604 ± 0.367
0.292HisHis: 0.292 ± 0.164
0.292HisIle: 0.292 ± 0.138
0.729HisLys: 0.729 ± 0.204
1.385HisLeu: 1.385 ± 0.379
0.146HisMet: 0.146 ± 0.101
0.364HisAsn: 0.364 ± 0.16
2.041HisPro: 2.041 ± 0.36
0.219HisGln: 0.219 ± 0.112
1.02HisArg: 1.02 ± 0.256
0.51HisSer: 0.51 ± 0.202
0.656HisThr: 0.656 ± 0.288
1.458HisVal: 1.458 ± 0.312
0.292HisTrp: 0.292 ± 0.127
1.239HisTyr: 1.239 ± 0.321
0.0HisXaa: 0.0 ± 0.0
Ile
4.811IleAla: 4.811 ± 0.545
0.146IleCys: 0.146 ± 0.113
3.426IleAsp: 3.426 ± 0.493
2.624IleGlu: 2.624 ± 0.522
0.729IlePhe: 0.729 ± 0.226
4.155IleGly: 4.155 ± 0.803
0.948IleHis: 0.948 ± 0.273
1.458IleIle: 1.458 ± 0.336
1.968IleLys: 1.968 ± 0.542
2.697IleLeu: 2.697 ± 0.444
0.583IleMet: 0.583 ± 0.245
1.239IleAsn: 1.239 ± 0.338
2.333IlePro: 2.333 ± 0.43
2.187IleGln: 2.187 ± 0.327
3.134IleArg: 3.134 ± 0.552
1.531IleSer: 1.531 ± 0.294
3.936IleThr: 3.936 ± 0.554
2.77IleVal: 2.77 ± 0.503
1.749IleTrp: 1.749 ± 0.573
0.948IleTyr: 0.948 ± 0.237
0.0IleXaa: 0.0 ± 0.0
Lys
5.904LysAla: 5.904 ± 1.141
0.292LysCys: 0.292 ± 0.129
2.26LysAsp: 2.26 ± 0.348
2.333LysGlu: 2.333 ± 0.442
1.385LysPhe: 1.385 ± 0.29
3.426LysGly: 3.426 ± 0.498
0.729LysHis: 0.729 ± 0.224
2.697LysIle: 2.697 ± 0.525
2.114LysLys: 2.114 ± 0.382
4.228LysLeu: 4.228 ± 0.463
1.166LysMet: 1.166 ± 0.241
1.677LysAsn: 1.677 ± 0.393
2.77LysPro: 2.77 ± 0.506
0.875LysGln: 0.875 ± 0.216
2.478LysArg: 2.478 ± 0.485
2.77LysSer: 2.77 ± 0.417
2.916LysThr: 2.916 ± 0.584
2.989LysVal: 2.989 ± 0.464
0.875LysTrp: 0.875 ± 0.226
1.458LysTyr: 1.458 ± 0.364
0.0LysXaa: 0.0 ± 0.0
Leu
7.872LeuAla: 7.872 ± 0.652
0.656LeuCys: 0.656 ± 0.28
6.196LeuAsp: 6.196 ± 0.748
4.082LeuGlu: 4.082 ± 0.5
2.478LeuPhe: 2.478 ± 0.392
6.342LeuGly: 6.342 ± 0.565
0.875LeuHis: 0.875 ± 0.273
4.665LeuIle: 4.665 ± 0.64
2.843LeuLys: 2.843 ± 0.582
5.467LeuLeu: 5.467 ± 0.646
2.041LeuMet: 2.041 ± 0.485
2.551LeuAsn: 2.551 ± 0.423
3.353LeuPro: 3.353 ± 0.42
3.061LeuGln: 3.061 ± 0.464
5.248LeuArg: 5.248 ± 0.588
4.082LeuSer: 4.082 ± 0.564
5.175LeuThr: 5.175 ± 0.662
5.758LeuVal: 5.758 ± 0.583
1.385LeuTrp: 1.385 ± 0.518
2.114LeuTyr: 2.114 ± 0.332
0.0LeuXaa: 0.0 ± 0.0
Met
2.478MetAla: 2.478 ± 0.44
0.073MetCys: 0.073 ± 0.083
1.822MetAsp: 1.822 ± 0.355
1.093MetGlu: 1.093 ± 0.252
0.0MetPhe: 0.0 ± 0.0
1.458MetGly: 1.458 ± 0.311
0.364MetHis: 0.364 ± 0.162
0.802MetIle: 0.802 ± 0.184
0.51MetLys: 0.51 ± 0.219
1.604MetLeu: 1.604 ± 0.323
0.364MetMet: 0.364 ± 0.186
0.948MetAsn: 0.948 ± 0.273
2.041MetPro: 2.041 ± 0.348
0.583MetGln: 0.583 ± 0.203
1.677MetArg: 1.677 ± 0.352
1.385MetSer: 1.385 ± 0.255
1.385MetThr: 1.385 ± 0.268
1.968MetVal: 1.968 ± 0.347
0.219MetTrp: 0.219 ± 0.116
0.51MetTyr: 0.51 ± 0.207
0.0MetXaa: 0.0 ± 0.0
Asn
4.738AsnAla: 4.738 ± 0.602
0.219AsnCys: 0.219 ± 0.108
1.166AsnAsp: 1.166 ± 0.256
2.333AsnGlu: 2.333 ± 0.362
0.875AsnPhe: 0.875 ± 0.253
4.738AsnGly: 4.738 ± 0.752
0.364AsnHis: 0.364 ± 0.246
0.656AsnIle: 0.656 ± 0.243
1.166AsnLys: 1.166 ± 0.281
2.916AsnLeu: 2.916 ± 0.431
0.219AsnMet: 0.219 ± 0.114
1.02AsnAsn: 1.02 ± 0.316
2.551AsnPro: 2.551 ± 0.414
1.312AsnGln: 1.312 ± 0.28
2.26AsnArg: 2.26 ± 0.446
1.385AsnSer: 1.385 ± 0.368
1.968AsnThr: 1.968 ± 0.404
1.531AsnVal: 1.531 ± 0.297
1.166AsnTrp: 1.166 ± 0.286
0.802AsnTyr: 0.802 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
5.904ProAla: 5.904 ± 0.796
0.219ProCys: 0.219 ± 0.129
3.863ProAsp: 3.863 ± 0.58
5.613ProGlu: 5.613 ± 0.858
1.458ProPhe: 1.458 ± 0.311
5.831ProGly: 5.831 ± 0.797
0.729ProHis: 0.729 ± 0.249
2.187ProIle: 2.187 ± 0.542
3.572ProLys: 3.572 ± 0.735
2.77ProLeu: 2.77 ± 0.51
1.02ProMet: 1.02 ± 0.306
2.333ProAsn: 2.333 ± 0.418
3.426ProPro: 3.426 ± 0.796
1.822ProGln: 1.822 ± 0.466
2.333ProArg: 2.333 ± 0.327
3.28ProSer: 3.28 ± 0.509
4.519ProThr: 4.519 ± 0.656
4.082ProVal: 4.082 ± 0.519
0.948ProTrp: 0.948 ± 0.288
1.458ProTyr: 1.458 ± 0.279
0.0ProXaa: 0.0 ± 0.0
Gln
5.321GlnAla: 5.321 ± 0.703
0.364GlnCys: 0.364 ± 0.178
1.749GlnAsp: 1.749 ± 0.384
3.717GlnGlu: 3.717 ± 0.507
1.239GlnPhe: 1.239 ± 0.28
3.426GlnGly: 3.426 ± 0.444
0.51GlnHis: 0.51 ± 0.193
2.041GlnIle: 2.041 ± 0.354
2.187GlnLys: 2.187 ± 0.586
2.843GlnLeu: 2.843 ± 0.412
0.802GlnMet: 0.802 ± 0.199
1.604GlnAsn: 1.604 ± 0.29
0.875GlnPro: 0.875 ± 0.248
1.458GlnGln: 1.458 ± 0.28
1.604GlnArg: 1.604 ± 0.358
2.114GlnSer: 2.114 ± 0.364
2.26GlnThr: 2.26 ± 0.401
2.989GlnVal: 2.989 ± 0.443
1.239GlnTrp: 1.239 ± 0.286
1.166GlnTyr: 1.166 ± 0.297
0.0GlnXaa: 0.0 ± 0.0
Arg
5.394ArgAla: 5.394 ± 0.704
0.656ArgCys: 0.656 ± 0.22
3.426ArgAsp: 3.426 ± 0.449
4.301ArgGlu: 4.301 ± 0.705
1.531ArgPhe: 1.531 ± 0.358
3.717ArgGly: 3.717 ± 0.543
1.239ArgHis: 1.239 ± 0.245
2.551ArgIle: 2.551 ± 0.464
3.353ArgLys: 3.353 ± 0.536
4.155ArgLeu: 4.155 ± 0.661
1.312ArgMet: 1.312 ± 0.3
1.895ArgAsn: 1.895 ± 0.414
2.697ArgPro: 2.697 ± 0.394
2.697ArgGln: 2.697 ± 0.454
4.009ArgArg: 4.009 ± 0.523
2.989ArgSer: 2.989 ± 0.447
2.989ArgThr: 2.989 ± 0.386
6.487ArgVal: 6.487 ± 0.611
1.531ArgTrp: 1.531 ± 0.302
2.114ArgTyr: 2.114 ± 0.434
0.0ArgXaa: 0.0 ± 0.0
Ser
5.758SerAla: 5.758 ± 0.664
0.146SerCys: 0.146 ± 0.103
3.499SerAsp: 3.499 ± 0.562
3.572SerGlu: 3.572 ± 0.514
1.458SerPhe: 1.458 ± 0.342
5.686SerGly: 5.686 ± 0.545
0.51SerHis: 0.51 ± 0.22
1.822SerIle: 1.822 ± 0.46
2.187SerLys: 2.187 ± 0.33
3.134SerLeu: 3.134 ± 0.434
1.385SerMet: 1.385 ± 0.378
1.822SerAsn: 1.822 ± 0.368
3.572SerPro: 3.572 ± 0.595
1.385SerGln: 1.385 ± 0.291
3.134SerArg: 3.134 ± 0.52
2.989SerSer: 2.989 ± 0.543
4.301SerThr: 4.301 ± 0.646
3.936SerVal: 3.936 ± 0.477
1.895SerTrp: 1.895 ± 0.48
1.604SerTyr: 1.604 ± 0.309
0.0SerXaa: 0.0 ± 0.0
Thr
6.925ThrAla: 6.925 ± 0.75
0.437ThrCys: 0.437 ± 0.164
4.446ThrAsp: 4.446 ± 0.535
4.082ThrGlu: 4.082 ± 0.448
1.749ThrPhe: 1.749 ± 0.398
6.196ThrGly: 6.196 ± 0.586
0.802ThrHis: 0.802 ± 0.215
3.061ThrIle: 3.061 ± 0.437
2.989ThrLys: 2.989 ± 0.425
5.175ThrLeu: 5.175 ± 0.512
1.531ThrMet: 1.531 ± 0.3
2.333ThrAsn: 2.333 ± 0.437
5.175ThrPro: 5.175 ± 0.906
2.697ThrGln: 2.697 ± 0.492
4.082ThrArg: 4.082 ± 0.596
4.228ThrSer: 4.228 ± 0.692
3.79ThrThr: 3.79 ± 0.765
4.228ThrVal: 4.228 ± 0.479
1.749ThrTrp: 1.749 ± 0.409
1.02ThrTyr: 1.02 ± 0.218
0.0ThrXaa: 0.0 ± 0.0
Val
8.31ValAla: 8.31 ± 0.799
0.073ValCys: 0.073 ± 0.072
4.811ValAsp: 4.811 ± 0.662
5.03ValGlu: 5.03 ± 0.64
1.749ValPhe: 1.749 ± 0.313
5.102ValGly: 5.102 ± 0.772
1.385ValHis: 1.385 ± 0.236
2.916ValIle: 2.916 ± 0.424
3.28ValLys: 3.28 ± 0.457
5.904ValLeu: 5.904 ± 0.566
1.677ValMet: 1.677 ± 0.321
1.749ValAsn: 1.749 ± 0.343
3.645ValPro: 3.645 ± 0.663
2.989ValGln: 2.989 ± 0.465
4.446ValArg: 4.446 ± 0.561
4.884ValSer: 4.884 ± 0.709
5.102ValThr: 5.102 ± 0.603
5.54ValVal: 5.54 ± 0.79
1.677ValTrp: 1.677 ± 0.381
1.677ValTyr: 1.677 ± 0.374
0.0ValXaa: 0.0 ± 0.0
Trp
2.989TrpAla: 2.989 ± 0.664
0.073TrpCys: 0.073 ± 0.068
1.895TrpAsp: 1.895 ± 0.442
1.02TrpGlu: 1.02 ± 0.244
1.02TrpPhe: 1.02 ± 0.26
1.822TrpGly: 1.822 ± 0.352
0.292TrpHis: 0.292 ± 0.131
1.385TrpIle: 1.385 ± 0.347
0.802TrpLys: 0.802 ± 0.229
1.677TrpLeu: 1.677 ± 0.324
0.51TrpMet: 0.51 ± 0.211
1.385TrpAsn: 1.385 ± 0.575
0.656TrpPro: 0.656 ± 0.19
0.875TrpGln: 0.875 ± 0.249
1.531TrpArg: 1.531 ± 0.415
1.312TrpSer: 1.312 ± 0.29
2.041TrpThr: 2.041 ± 0.435
2.041TrpVal: 2.041 ± 0.366
0.583TrpTrp: 0.583 ± 0.247
0.875TrpTyr: 0.875 ± 0.248
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.77TyrAla: 2.77 ± 0.53
0.146TyrCys: 0.146 ± 0.112
1.749TyrAsp: 1.749 ± 0.285
1.968TyrGlu: 1.968 ± 0.402
0.875TyrPhe: 0.875 ± 0.187
2.405TyrGly: 2.405 ± 0.363
0.583TyrHis: 0.583 ± 0.183
0.729TyrIle: 0.729 ± 0.211
1.166TyrLys: 1.166 ± 0.387
2.405TyrLeu: 2.405 ± 0.359
0.364TyrMet: 0.364 ± 0.137
0.729TyrAsn: 0.729 ± 0.258
1.531TyrPro: 1.531 ± 0.276
1.458TyrGln: 1.458 ± 0.334
1.677TyrArg: 1.677 ± 0.357
1.239TyrSer: 1.239 ± 0.256
1.968TyrThr: 1.968 ± 0.381
2.26TyrVal: 2.26 ± 0.404
0.948TyrTrp: 0.948 ± 0.23
0.875TyrTyr: 0.875 ± 0.266
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (13720 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski