Paenibacillus dendritiformis C454

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; Paenibacillus dendritiformis

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5660 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H3SPR6|H3SPR6_9BACL Orn/Lys/Arg decarboxylase major region OS=Paenibacillus dendritiformis C454 OX=1131935 GN=PDENDC454_27868 PE=3 SV=1
MM1 pKa = 7.77SEE3 pKa = 3.97HH4 pKa = 5.45QHH6 pKa = 5.89NHH8 pKa = 5.9DD9 pKa = 4.09HH10 pKa = 7.84DD11 pKa = 4.82CGCGHH16 pKa = 6.58DD17 pKa = 4.46HH18 pKa = 6.53EE19 pKa = 6.97HH20 pKa = 7.6DD21 pKa = 3.83EE22 pKa = 4.85LVVTLVDD29 pKa = 3.61EE30 pKa = 4.85EE31 pKa = 4.71GNEE34 pKa = 3.43IDD36 pKa = 3.51MALVEE41 pKa = 4.35TFNVGEE47 pKa = 4.0QVYY50 pKa = 11.0ALLLEE55 pKa = 4.68RR56 pKa = 11.84NNPEE60 pKa = 3.38ADD62 pKa = 4.13GIIVRR67 pKa = 11.84VDD69 pKa = 3.42EE70 pKa = 4.55EE71 pKa = 4.93DD72 pKa = 3.33EE73 pKa = 5.09DD74 pKa = 4.12MVLNPIEE81 pKa = 5.68DD82 pKa = 3.75DD83 pKa = 4.09EE84 pKa = 4.31EE85 pKa = 4.42WEE87 pKa = 4.42RR88 pKa = 11.84VQQAYY93 pKa = 10.46DD94 pKa = 3.3QMVASQEE101 pKa = 4.16DD102 pKa = 3.45

Molecular weight: 11.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H3SKY6|H3SKY6_9BACL Histidine kinase OS=Paenibacillus dendritiformis C454 OX=1131935 GN=PDENDC454_21102 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 8.7PTFKK6 pKa = 10.81PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.2NGRR28 pKa = 11.84KK29 pKa = 9.14VIAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5660

0

5660

1785742

22

3077

315.5

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.081 ± 0.048

0.872 ± 0.01

5.168 ± 0.025

6.969 ± 0.037

4.002 ± 0.026

7.485 ± 0.031

2.274 ± 0.018

6.398 ± 0.029

4.816 ± 0.031

9.995 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.987 ± 0.016

3.345 ± 0.022

4.289 ± 0.022

3.904 ± 0.021

5.816 ± 0.028

5.8 ± 0.024

5.071 ± 0.024

6.932 ± 0.027

1.334 ± 0.014

3.464 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski