Geofilum rubicundum JCM 15548

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Marinilabiliaceae; Geofilum; Geofilum rubicundum

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4411 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E9LYJ4|A0A0E9LYJ4_9BACT Uncharacterized protein OS=Geofilum rubicundum JCM 15548 OX=1236989 GN=JCM15548_12462 PE=4 SV=1
MM1 pKa = 7.32AHH3 pKa = 7.44DD4 pKa = 4.1EE5 pKa = 4.57GEE7 pKa = 4.35VYY9 pKa = 10.83LIPEE13 pKa = 4.71FDD15 pKa = 4.44HH16 pKa = 7.3PDD18 pKa = 3.43DD19 pKa = 6.32AIDD22 pKa = 3.72FLKK25 pKa = 10.51DD26 pKa = 3.14YY27 pKa = 10.29YY28 pKa = 11.6VEE30 pKa = 4.13IFEE33 pKa = 4.83HH34 pKa = 6.62QLFSWITDD42 pKa = 3.54DD43 pKa = 6.07NLWPDD48 pKa = 3.47NLSWEE53 pKa = 4.5LFNSWFHH60 pKa = 6.1YY61 pKa = 10.21SIQSMVMDD69 pKa = 4.04TLGEE73 pKa = 4.17EE74 pKa = 4.07IEE76 pKa = 4.2KK77 pKa = 11.21DD78 pKa = 3.51EE79 pKa = 4.37FF80 pKa = 5.1

Molecular weight: 9.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E9M188|A0A0E9M188_9BACT Putative secreted protein OS=Geofilum rubicundum JCM 15548 OX=1236989 GN=JCM15548_13203 PE=4 SV=1
MM1 pKa = 7.84VYY3 pKa = 10.6DD4 pKa = 4.51GVLVSPDD11 pKa = 3.27VPLLHH16 pKa = 5.96STNRR20 pKa = 11.84AFRR23 pKa = 11.84YY24 pKa = 10.18GDD26 pKa = 3.48GLFEE30 pKa = 4.65TIRR33 pKa = 11.84FHH35 pKa = 8.42KK36 pKa = 10.6GEE38 pKa = 4.21PLHH41 pKa = 6.6WEE43 pKa = 3.65SHH45 pKa = 4.3YY46 pKa = 10.97QRR48 pKa = 11.84LLKK51 pKa = 10.68GMAVLKK57 pKa = 9.09MASGSFPSRR66 pKa = 11.84QSMRR70 pKa = 11.84DD71 pKa = 3.42SIIDD75 pKa = 3.63LVVKK79 pKa = 10.62NRR81 pKa = 11.84IFADD85 pKa = 2.95ARR87 pKa = 11.84VRR89 pKa = 11.84LTVFRR94 pKa = 11.84KK95 pKa = 10.5GEE97 pKa = 3.85GSTRR101 pKa = 11.84RR102 pKa = 11.84IEE104 pKa = 4.16CRR106 pKa = 11.84CRR108 pKa = 11.84GSSKK112 pKa = 10.2PRR114 pKa = 11.84IFTRR118 pKa = 11.84RR119 pKa = 11.84DD120 pKa = 2.41IHH122 pKa = 6.46FPKK125 pKa = 10.17RR126 pKa = 11.84DD127 pKa = 3.22

Molecular weight: 14.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4411

0

4411

1419689

37

2737

321.9

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.067 ± 0.035

0.73 ± 0.013

5.548 ± 0.026

6.638 ± 0.031

4.95 ± 0.03

7.033 ± 0.032

2.145 ± 0.018

6.768 ± 0.035

5.796 ± 0.039

9.64 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.017

5.049 ± 0.031

3.946 ± 0.022

3.763 ± 0.023

4.525 ± 0.024

6.523 ± 0.028

5.313 ± 0.03

6.715 ± 0.029

1.333 ± 0.018

3.874 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski