Penaeus monodon circovirus VN11
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U6BMM7|U6BMM7_9CIRC Nonstructural protein 2 OS=Penaeus monodon circovirus VN11 OX=1419711 PE=4 SV=1
MM1 pKa = 7.47 VVEE4 pKa = 4.29 PAGDD8 pKa = 3.61 HH9 pKa = 6.13 LRR11 pKa = 11.84 AARR14 pKa = 11.84 AGHH17 pKa = 6.3 AGWGEE22 pKa = 3.84 EE23 pKa = 3.91 GLNLNDD29 pKa = 3.77 CRR31 pKa = 11.84 QDD33 pKa = 3.29 AVLVKK38 pKa = 9.98 QAAKK42 pKa = 10.6 LLVVDD47 pKa = 6.01 DD48 pKa = 3.66 ISEE51 pKa = 4.26 RR52 pKa = 11.84 KK53 pKa = 10.0 DD54 pKa = 3.22 EE55 pKa = 4.42 SLVSTVASDD64 pKa = 3.56 CDD66 pKa = 4.01 TRR68 pKa = 11.84 CAPAANLLGPPVVRR82 pKa = 11.84 LDD84 pKa = 3.58 ALEE87 pKa = 4.0 VANIRR92 pKa = 11.84 VAGEE96 pKa = 3.95 PVHH99 pKa = 5.83 VAVVEE104 pKa = 3.95 LACLCGHH111 pKa = 7.19 ALVALLLVVALLPIRR126 pKa = 11.84 AAKK129 pKa = 10.42 ASLCCVRR136 pKa = 11.84 APAEE140 pKa = 4.03 RR141 pKa = 11.84 HH142 pKa = 5.71 HH143 pKa = 7.11 LSLL146 pKa = 5.55
Molecular weight: 15.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.593
IPC2_protein 5.55
IPC_protein 5.55
Toseland 5.893
ProMoST 5.906
Dawson 5.804
Bjellqvist 5.817
Wikipedia 5.817
Rodwell 5.792
Grimsley 5.995
Solomon 5.804
Lehninger 5.804
Nozaki 6.071
DTASelect 6.262
Thurlkill 6.262
EMBOSS 6.224
Sillero 6.173
Patrickios 4.062
IPC_peptide 5.83
IPC2_peptide 6.198
IPC2.peptide.svr19 6.141
Protein with the highest isoelectric point:
>tr|U6BML2|U6BML2_9CIRC Replication-associated protein OS=Penaeus monodon circovirus VN11 OX=1419711 PE=4 SV=1
MM1 pKa = 7.66 PLRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 TYY8 pKa = 6.76 TAKK11 pKa = 10.24 RR12 pKa = 11.84 RR13 pKa = 11.84 FGRR16 pKa = 11.84 SYY18 pKa = 10.85 RR19 pKa = 11.84 KK20 pKa = 9.56 KK21 pKa = 10.13 SYY23 pKa = 10.39 YY24 pKa = 9.72 KK25 pKa = 9.23 KK26 pKa = 10.38 KK27 pKa = 9.92 SYY29 pKa = 11.0 KK30 pKa = 10.29 RR31 pKa = 11.84 MAAKK35 pKa = 9.87 ARR37 pKa = 11.84 KK38 pKa = 8.18 FNNGNVHH45 pKa = 7.04 RR46 pKa = 11.84 FTRR49 pKa = 11.84 YY50 pKa = 9.79 SNVGYY55 pKa = 9.66 LEE57 pKa = 4.43 CVQPYY62 pKa = 10.38 DD63 pKa = 2.87 WGAQEE68 pKa = 4.23 VGGGGAPGVAITGHH82 pKa = 6.41 CANKK86 pKa = 10.44 AFVFSLTDD94 pKa = 3.28 VVHH97 pKa = 6.77 NEE99 pKa = 3.9 EE100 pKa = 4.55 FGSLFDD106 pKa = 3.66 QYY108 pKa = 11.03 RR109 pKa = 11.84 ILSAVVEE116 pKa = 4.32 IQPLLTPASMTGTGGTQMIARR137 pKa = 11.84 WFYY140 pKa = 11.7 DD141 pKa = 3.18 HH142 pKa = 7.89 DD143 pKa = 5.45 DD144 pKa = 4.13 DD145 pKa = 5.12 STLGMSGADD154 pKa = 3.12 ADD156 pKa = 4.58 EE157 pKa = 4.43 YY158 pKa = 10.04 WGQRR162 pKa = 11.84 APLVKK167 pKa = 9.29 EE168 pKa = 4.02 TPFRR172 pKa = 11.84 FDD174 pKa = 3.31 RR175 pKa = 11.84 PIVIPVIPTTQTMTPQDD192 pKa = 4.45 DD193 pKa = 4.34 GNVYY197 pKa = 10.82 APVPGSTYY205 pKa = 10.53 KK206 pKa = 10.63 KK207 pKa = 10.58 RR208 pKa = 11.84 MWLDD212 pKa = 2.97 MTFPDD217 pKa = 4.13 VPHH220 pKa = 6.74 YY221 pKa = 10.45 GLKK224 pKa = 10.28 FQVLRR229 pKa = 11.84 RR230 pKa = 11.84 AQLAAEE236 pKa = 4.39 VGPIAQYY243 pKa = 9.98 RR244 pKa = 11.84 IKK246 pKa = 11.2 YY247 pKa = 9.28 NLEE250 pKa = 3.84 FKK252 pKa = 10.83 NPRR255 pKa = 3.35
Molecular weight: 29.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.843
IPC2_protein 9.311
IPC_protein 9.37
Toseland 9.677
ProMoST 9.604
Dawson 9.999
Bjellqvist 9.736
Wikipedia 10.218
Rodwell 10.204
Grimsley 10.101
Solomon 10.014
Lehninger 9.97
Nozaki 9.706
DTASelect 9.721
Thurlkill 9.809
EMBOSS 10.116
Sillero 9.911
Patrickios 4.902
IPC_peptide 10.014
IPC2_peptide 8.448
IPC2.peptide.svr19 8.227
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
667
146
266
222.3
25.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.096 ± 3.216
2.099 ± 0.892
5.247 ± 0.409
6.297 ± 1.483
3.748 ± 1.2
7.346 ± 0.615
2.849 ± 0.475
4.048 ± 0.887
6.297 ± 1.297
7.946 ± 2.278
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.849 ± 0.726
3.448 ± 0.24
5.697 ± 0.458
2.999 ± 0.665
6.747 ± 0.803
4.348 ± 0.096
5.547 ± 1.342
8.246 ± 1.771
1.949 ± 0.582
4.198 ± 1.393
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here