Proteome-pI 2.0 - Proteome Isoelectric Point Database

Shuangao insect virus 10

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1L3KFV2|A0A1L3KFV2_9VIRU RNA replicase OS=Shuangao insect virus 10 OX=1923466 PE=3 SV=1

MM1 pKa = 7.86KK2 pKa = 10.66LFTPRR7 pKa = 11.84NNHH10 pKa = 5.01SHH12 pKa = 6.45PTAAMYY18 pKa = 10.48RR19 pKa = 11.84SGVNAFMTRR28 pKa = 11.84LVTSAGFIPYY38 pKa = 8.62QVSMSATDD46 pKa = 3.18KK47 pKa = 11.35CDD49 pKa = 3.3GTRR52 pKa = 11.84LFYY55 pKa = 10.48GQKK58 pKa = 10.63DD59 pKa = 3.17LAIPYY64 pKa = 9.63KK65 pKa = 10.09HH66 pKa = 7.17DD67 pKa = 3.7SVKK70 pKa = 10.3DD71 pKa = 3.13QHH73 pKa = 7.78VIIMTDD79 pKa = 2.41VDD81 pKa = 4.66YY82 pKa = 10.58YY83 pKa = 11.18TDD85 pKa = 3.4INKK88 pKa = 8.88WLSYY92 pKa = 10.09GRR94 pKa = 11.84PILMYY99 pKa = 9.93TMVPKK104 pKa = 10.03TVVGRR109 pKa = 11.84TQDD112 pKa = 3.3YY113 pKa = 10.32AYY115 pKa = 10.43RR116 pKa = 11.84IVNGRR121 pKa = 11.84LEE123 pKa = 3.92MHH125 pKa = 6.23VAGGSTYY132 pKa = 7.52THH134 pKa = 6.48EE135 pKa = 4.07LWDD138 pKa = 3.97YY139 pKa = 11.43QGDD142 pKa = 4.05TVSIVAEE149 pKa = 4.06DD150 pKa = 3.85GKK152 pKa = 10.8NNEE155 pKa = 4.2HH156 pKa = 6.6VICFHH161 pKa = 6.83LEE163 pKa = 3.69QRR165 pKa = 11.84DD166 pKa = 3.79IPGDD170 pKa = 3.8PDD172 pKa = 3.27HH173 pKa = 6.93KK174 pKa = 10.81FIVFTPYY181 pKa = 10.85AYY183 pKa = 9.62IPAPYY188 pKa = 9.58YY189 pKa = 8.8YY190 pKa = 10.69VCIEE194 pKa = 3.94PTPVRR199 pKa = 11.84RR200 pKa = 11.84KK201 pKa = 10.49CFGDD205 pKa = 3.59TINYY209 pKa = 9.24LFEE212 pKa = 6.01PITNTLSISEE222 pKa = 4.14NGSWNSVEE230 pKa = 5.41LDD232 pKa = 3.14ATLYY236 pKa = 10.89SAIQQRR242 pKa = 11.84LLQKK246 pKa = 7.17TTPTLIVDD254 pKa = 3.85VEE256 pKa = 4.17RR257 pKa = 11.84HH258 pKa = 4.98LRR260 pKa = 11.84AAEE263 pKa = 3.96HH264 pKa = 6.4PRR266 pKa = 11.84PAVAAPLVYY275 pKa = 10.59QLMNCTIDD283 pKa = 3.54KK284 pKa = 10.39NVVMTKK290 pKa = 10.56GCITTYY296 pKa = 10.56HH297 pKa = 6.87PLGTLVNEE305 pKa = 4.71DD306 pKa = 4.17GKK308 pKa = 10.16PSGSQVCTPMLTEE321 pKa = 4.7AGVFPSNSVASDD333 pKa = 3.2EE334 pKa = 4.21ATVRR338 pKa = 11.84GRR340 pKa = 11.84IDD342 pKa = 3.04KK343 pKa = 10.22VRR345 pKa = 11.84NNVVPPRR352 pKa = 11.84KK353 pKa = 9.75YY354 pKa = 10.56NVWRR358 pKa = 11.84NDD360 pKa = 3.72FITTIVKK367 pKa = 10.27HH368 pKa = 6.43PGTGGPIGTGDD379 pKa = 3.33VAEE382 pKa = 4.44VQNKK386 pKa = 8.4PMQRR390 pKa = 11.84ARR392 pKa = 11.84FVKK395 pKa = 10.16SEE397 pKa = 4.3CTLTTTPINEE407 pKa = 4.14LKK409 pKa = 10.88SFVKK413 pKa = 10.54VEE415 pKa = 4.49PYY417 pKa = 10.5TSINDD422 pKa = 3.46PRR424 pKa = 11.84NITTMIPEE432 pKa = 4.69LTTEE436 pKa = 4.44LSAYY440 pKa = 10.02SIPFKK445 pKa = 11.29DD446 pKa = 4.11EE447 pKa = 3.88VLKK450 pKa = 10.78DD451 pKa = 3.95LPWYY455 pKa = 10.5GPGKK459 pKa = 8.92TPIQAVEE466 pKa = 4.27KK467 pKa = 8.16LQEE470 pKa = 4.01MAMSDD475 pKa = 3.89KK476 pKa = 10.56PWIVTDD482 pKa = 4.22FSRR485 pKa = 11.84MDD487 pKa = 3.38GTISEE492 pKa = 4.35WMQHH496 pKa = 5.57FLTEE500 pKa = 4.29IMLRR504 pKa = 11.84WVRR507 pKa = 11.84DD508 pKa = 3.46EE509 pKa = 4.11YY510 pKa = 11.63KK511 pKa = 11.27DD512 pKa = 3.32MLRR515 pKa = 11.84YY516 pKa = 9.85LLLQVLIMKK525 pKa = 9.88GRR527 pKa = 11.84TKK529 pKa = 10.55HH530 pKa = 5.7GIKK533 pKa = 9.98YY534 pKa = 9.27QPGWGNRR541 pKa = 11.84SGSPVTTILNTLVSILIVYY560 pKa = 7.67MALRR564 pKa = 11.84SLGQNHH570 pKa = 5.62NKK572 pKa = 8.44AWSMIQFYY580 pKa = 9.52CLKK583 pKa = 10.51YY584 pKa = 10.74GDD586 pKa = 5.04DD587 pKa = 4.88GVDD590 pKa = 3.29ILCVEE595 pKa = 4.52GLDD598 pKa = 4.57DD599 pKa = 6.44AITSVAEE606 pKa = 4.02DD607 pKa = 3.93LGLKK611 pKa = 9.96IKK613 pKa = 10.64IEE615 pKa = 4.49VIPMGCTIPYY625 pKa = 8.88LGRR628 pKa = 11.84YY629 pKa = 8.21FVDD632 pKa = 4.29PSSMPDD638 pKa = 3.12SFQDD642 pKa = 3.52PLRR645 pKa = 11.84TIDD648 pKa = 4.48KK649 pKa = 10.45LHH651 pKa = 6.8LSSNTQVTARR661 pKa = 11.84QAQFNKK667 pKa = 9.74ASGYY671 pKa = 8.15ITTDD675 pKa = 2.69RR676 pKa = 11.84LTPIIGSYY684 pKa = 10.33CKK686 pKa = 10.52VVLDD690 pKa = 4.71SYY692 pKa = 11.78AGMSVRR698 pKa = 11.84GEE700 pKa = 4.19TSEE703 pKa = 4.51EE704 pKa = 4.05RR705 pKa = 11.84YY706 pKa = 10.53KK707 pKa = 10.57MSNAWPQYY715 pKa = 9.02EE716 pKa = 4.13SHH718 pKa = 6.89KK719 pKa = 10.89NHH721 pKa = 6.37ITQSMAEE728 pKa = 4.13LLKK731 pKa = 10.39IEE733 pKa = 3.97YY734 pKa = 10.15SEE736 pKa = 4.58LKK738 pKa = 10.53RR739 pKa = 11.84LDD741 pKa = 3.64EE742 pKa = 4.2MVSNVTNPEE751 pKa = 3.86EE752 pKa = 4.13FPVLFEE758 pKa = 4.04KK759 pKa = 10.12TMKK762 pKa = 10.5VNLPAIYY769 pKa = 10.59GEE771 pKa = 4.39VIVEE775 pKa = 4.05QGEE778 pKa = 4.95HH779 pKa = 6.43YY780 pKa = 10.86DD781 pKa = 3.61QDD783 pKa = 5.01DD784 pKa = 3.72IQSAAVRR791 pKa = 11.84PQSQPASDD799 pKa = 3.59QTSGGTTDD807 pKa = 3.44NSQGHH812 pKa = 6.61AEE814 pKa = 4.03QVARR818 pKa = 11.84PKK820 pKa = 10.62SKK822 pKa = 10.14RR823 pKa = 11.84RR824 pKa = 11.84PNRR827 pKa = 11.84GRR829 pKa = 11.84CSNTKK834 pKa = 6.76EE835 pKa = 4.01QRR837 pKa = 11.84GGASPNKK844 pKa = 10.12ANPKK848 pKa = 9.05PNARR852 pKa = 11.84SAKK855 pKa = 8.93QRR857 pKa = 11.84VARR860 pKa = 11.84GSRR863 pKa = 11.84RR864 pKa = 11.84QVV866 pKa = 2.68

Molecular weight: 97.67 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1L3KFV2|A0A1L3KFV2_9VIRU RNA replicase OS=Shuangao insect virus 10 OX=1923466 PE=3 SV=1

MM1 pKa = 7.86KK2 pKa = 10.66LFTPRR7 pKa = 11.84NNHH10 pKa = 5.01SHH12 pKa = 6.45PTAAMYY18 pKa = 10.48RR19 pKa = 11.84SGVNAFMTRR28 pKa = 11.84LVTSAGFIPYY38 pKa = 8.62QVSMSATDD46 pKa = 3.18KK47 pKa = 11.35CDD49 pKa = 3.3GTRR52 pKa = 11.84LFYY55 pKa = 10.48GQKK58 pKa = 10.63DD59 pKa = 3.17LAIPYY64 pKa = 9.63KK65 pKa = 10.09HH66 pKa = 7.17DD67 pKa = 3.7SVKK70 pKa = 10.3DD71 pKa = 3.13QHH73 pKa = 7.78VIIMTDD79 pKa = 2.41VDD81 pKa = 4.66YY82 pKa = 10.58YY83 pKa = 11.18TDD85 pKa = 3.4INKK88 pKa = 8.88WLSYY92 pKa = 10.09GRR94 pKa = 11.84PILMYY99 pKa = 9.93TMVPKK104 pKa = 10.03TVVGRR109 pKa = 11.84TQDD112 pKa = 3.3YY113 pKa = 10.32AYY115 pKa = 10.43RR116 pKa = 11.84IVNGRR121 pKa = 11.84LEE123 pKa = 3.92MHH125 pKa = 6.23VAGGSTYY132 pKa = 7.52THH134 pKa = 6.48EE135 pKa = 4.07LWDD138 pKa = 3.97YY139 pKa = 11.43QGDD142 pKa = 4.05TVSIVAEE149 pKa = 4.06DD150 pKa = 3.85GKK152 pKa = 10.8NNEE155 pKa = 4.2HH156 pKa = 6.6VICFHH161 pKa = 6.83LEE163 pKa = 3.69QRR165 pKa = 11.84DD166 pKa = 3.79IPGDD170 pKa = 3.8PDD172 pKa = 3.27HH173 pKa = 6.93KK174 pKa = 10.81FIVFTPYY181 pKa = 10.85AYY183 pKa = 9.62IPAPYY188 pKa = 9.58YY189 pKa = 8.8YY190 pKa = 10.69VCIEE194 pKa = 3.94PTPVRR199 pKa = 11.84RR200 pKa = 11.84KK201 pKa = 10.49CFGDD205 pKa = 3.59TINYY209 pKa = 9.24LFEE212 pKa = 6.01PITNTLSISEE222 pKa = 4.14NGSWNSVEE230 pKa = 5.41LDD232 pKa = 3.14ATLYY236 pKa = 10.89SAIQQRR242 pKa = 11.84LLQKK246 pKa = 7.17TTPTLIVDD254 pKa = 3.85VEE256 pKa = 4.17RR257 pKa = 11.84HH258 pKa = 4.98LRR260 pKa = 11.84AAEE263 pKa = 3.96HH264 pKa = 6.4PRR266 pKa = 11.84PAVAAPLVYY275 pKa = 10.59QLMNCTIDD283 pKa = 3.54KK284 pKa = 10.39NVVMTKK290 pKa = 10.56GCITTYY296 pKa = 10.56HH297 pKa = 6.87PLGTLVNEE305 pKa = 4.71DD306 pKa = 4.17GKK308 pKa = 10.16PSGSQVCTPMLTEE321 pKa = 4.7AGVFPSNSVASDD333 pKa = 3.2EE334 pKa = 4.21ATVRR338 pKa = 11.84GRR340 pKa = 11.84IDD342 pKa = 3.04KK343 pKa = 10.22VRR345 pKa = 11.84NNVVPPRR352 pKa = 11.84KK353 pKa = 9.75YY354 pKa = 10.56NVWRR358 pKa = 11.84NDD360 pKa = 3.72FITTIVKK367 pKa = 10.27HH368 pKa = 6.43PGTGGPIGTGDD379 pKa = 3.33VAEE382 pKa = 4.44VQNKK386 pKa = 8.4PMQRR390 pKa = 11.84ARR392 pKa = 11.84FVKK395 pKa = 10.16SEE397 pKa = 4.3CTLTTTPINEE407 pKa = 4.14LKK409 pKa = 10.88SFVKK413 pKa = 10.54VEE415 pKa = 4.49PYY417 pKa = 10.5TSINDD422 pKa = 3.46PRR424 pKa = 11.84NITTMIPEE432 pKa = 4.69LTTEE436 pKa = 4.44LSAYY440 pKa = 10.02SIPFKK445 pKa = 11.29DD446 pKa = 4.11EE447 pKa = 3.88VLKK450 pKa = 10.78DD451 pKa = 3.95LPWYY455 pKa = 10.5GPGKK459 pKa = 8.92TPIQAVEE466 pKa = 4.27KK467 pKa = 8.16LQEE470 pKa = 4.01MAMSDD475 pKa = 3.89KK476 pKa = 10.56PWIVTDD482 pKa = 4.22FSRR485 pKa = 11.84MDD487 pKa = 3.38GTISEE492 pKa = 4.35WMQHH496 pKa = 5.57FLTEE500 pKa = 4.29IMLRR504 pKa = 11.84WVRR507 pKa = 11.84DD508 pKa = 3.46EE509 pKa = 4.11YY510 pKa = 11.63KK511 pKa = 11.27DD512 pKa = 3.32MLRR515 pKa = 11.84YY516 pKa = 9.85LLLQVLIMKK525 pKa = 9.88GRR527 pKa = 11.84TKK529 pKa = 10.55HH530 pKa = 5.7GIKK533 pKa = 9.98YY534 pKa = 9.27QPGWGNRR541 pKa = 11.84SGSPVTTILNTLVSILIVYY560 pKa = 7.67MALRR564 pKa = 11.84SLGQNHH570 pKa = 5.62NKK572 pKa = 8.44AWSMIQFYY580 pKa = 9.52CLKK583 pKa = 10.51YY584 pKa = 10.74GDD586 pKa = 5.04DD587 pKa = 4.88GVDD590 pKa = 3.29ILCVEE595 pKa = 4.52GLDD598 pKa = 4.57DD599 pKa = 6.44AITSVAEE606 pKa = 4.02DD607 pKa = 3.93LGLKK611 pKa = 9.96IKK613 pKa = 10.64IEE615 pKa = 4.49VIPMGCTIPYY625 pKa = 8.88LGRR628 pKa = 11.84YY629 pKa = 8.21FVDD632 pKa = 4.29PSSMPDD638 pKa = 3.12SFQDD642 pKa = 3.52PLRR645 pKa = 11.84TIDD648 pKa = 4.48KK649 pKa = 10.45LHH651 pKa = 6.8LSSNTQVTARR661 pKa = 11.84QAQFNKK667 pKa = 9.74ASGYY671 pKa = 8.15ITTDD675 pKa = 2.69RR676 pKa = 11.84LTPIIGSYY684 pKa = 10.33CKK686 pKa = 10.52VVLDD690 pKa = 4.71SYY692 pKa = 11.78AGMSVRR698 pKa = 11.84GEE700 pKa = 4.19TSEE703 pKa = 4.51EE704 pKa = 4.05RR705 pKa = 11.84YY706 pKa = 10.53KK707 pKa = 10.57MSNAWPQYY715 pKa = 9.02EE716 pKa = 4.13SHH718 pKa = 6.89KK719 pKa = 10.89NHH721 pKa = 6.37ITQSMAEE728 pKa = 4.13LLKK731 pKa = 10.39IEE733 pKa = 3.97YY734 pKa = 10.15SEE736 pKa = 4.58LKK738 pKa = 10.53RR739 pKa = 11.84LDD741 pKa = 3.64EE742 pKa = 4.2MVSNVTNPEE751 pKa = 3.86EE752 pKa = 4.13FPVLFEE758 pKa = 4.04KK759 pKa = 10.12TMKK762 pKa = 10.5VNLPAIYY769 pKa = 10.59GEE771 pKa = 4.39VIVEE775 pKa = 4.05QGEE778 pKa = 4.95HH779 pKa = 6.43YY780 pKa = 10.86DD781 pKa = 3.61QDD783 pKa = 5.01DD784 pKa = 3.72IQSAAVRR791 pKa = 11.84PQSQPASDD799 pKa = 3.59QTSGGTTDD807 pKa = 3.44NSQGHH812 pKa = 6.61AEE814 pKa = 4.03QVARR818 pKa = 11.84PKK820 pKa = 10.62SKK822 pKa = 10.14RR823 pKa = 11.84RR824 pKa = 11.84PNRR827 pKa = 11.84GRR829 pKa = 11.84CSNTKK834 pKa = 6.76EE835 pKa = 4.01QRR837 pKa = 11.84GGASPNKK844 pKa = 10.12ANPKK848 pKa = 9.05PNARR852 pKa = 11.84SAKK855 pKa = 8.93QRR857 pKa = 11.84VARR860 pKa = 11.84GSRR863 pKa = 11.84RR864 pKa = 11.84QVV866 pKa = 2.68

Molecular weight: 97.67 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	866	866	866	866.0	97.67

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
5.427 ± 0.0	1.501 ± 0.0	5.774 ± 0.0	5.196 ± 0.0	2.54 ± 0.0	6.12 ± 0.0	2.425 ± 0.0	6.236 ± 0.0	5.658 ± 0.0	6.697 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
3.349 ± 0.0	4.619 ± 0.0	6.351 ± 0.0	4.273 ± 0.0	5.658 ± 0.0	6.697 ± 0.0	7.852 ± 0.0	7.621 ± 0.0	1.27 ± 0.0	4.734 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski