Pomona leaf-nosed bat associated polyomavirus
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R3SYE4|A0A1R3SYE4_9POLY Late orf 1 OS=Pomona leaf-nosed bat associated polyomavirus OX=1885565 GN=LO1 PE=4 SV=1
MM1 pKa = 7.63 GIVASIVAAVTGVAEE16 pKa = 4.29 AVADD20 pKa = 4.0 AAATVAGAAGSAAEE34 pKa = 3.91 AAATSFEE41 pKa = 4.42 LTSSVGEE48 pKa = 4.07 AAGDD52 pKa = 3.85 TVPLLDD58 pKa = 4.45 GEE60 pKa = 4.54 EE61 pKa = 4.04 EE62 pKa = 4.68 TVFDD66 pKa = 4.48 SSWAEE71 pKa = 3.39 GGYY74 pKa = 10.65 ADD76 pKa = 4.74 EE77 pKa = 5.39 GAVNDD82 pKa = 3.65 PWNWEE87 pKa = 4.01 MEE89 pKa = 4.34 PEE91 pKa = 4.15 DD92 pKa = 4.32 LATGSGSRR100 pKa = 11.84 LSRR103 pKa = 11.84 SLATGLAAGAGVAAAAGGLAWAIGSAAASHH133 pKa = 6.33 SEE135 pKa = 3.75 AGQALLEE142 pKa = 4.21 SSQVIQRR149 pKa = 11.84 TLEE152 pKa = 4.02 EE153 pKa = 4.32 EE154 pKa = 4.77 GEE156 pKa = 4.34 QPSVPDD162 pKa = 3.12 IVMGFMTPEE171 pKa = 3.78 EE172 pKa = 4.24 YY173 pKa = 10.39 EE174 pKa = 3.97 RR175 pKa = 11.84 ALEE178 pKa = 4.05 EE179 pKa = 3.59 LHH181 pKa = 6.54 RR182 pKa = 11.84 RR183 pKa = 11.84 QVEE186 pKa = 4.43 GGFSPDD192 pKa = 3.1 SLRR195 pKa = 11.84 EE196 pKa = 3.98 AEE198 pKa = 4.12 NALVYY203 pKa = 10.17 IAPGSGIPYY212 pKa = 9.61 RR213 pKa = 11.84 VPDD216 pKa = 3.58 QSLLMDD222 pKa = 4.41 MAQSEE227 pKa = 4.65 QPAPWFLPGAAGQPMTRR244 pKa = 11.84 GMLRR248 pKa = 11.84 RR249 pKa = 11.84 AAEE252 pKa = 3.98 AARR255 pKa = 11.84 VIAEE259 pKa = 4.04 GAEE262 pKa = 3.85 RR263 pKa = 11.84 SQVFRR268 pKa = 11.84 LSADD272 pKa = 2.92 ARR274 pKa = 11.84 RR275 pKa = 11.84 EE276 pKa = 3.93 LARR279 pKa = 11.84 SGQALADD286 pKa = 4.22 LYY288 pKa = 11.18 RR289 pKa = 11.84 RR290 pKa = 11.84 GLRR293 pKa = 11.84 PSDD296 pKa = 3.39 LLYY299 pKa = 10.76 RR300 pKa = 11.84 QVLRR304 pKa = 11.84 GNRR307 pKa = 11.84 QVQEE311 pKa = 4.41 DD312 pKa = 3.41 IARR315 pKa = 11.84 RR316 pKa = 11.84 VAAEE320 pKa = 3.78 IAGQALDD327 pKa = 3.53 IGIEE331 pKa = 3.88 QGQQIVGNLIGSGVIATSLAAALTGAGHH359 pKa = 7.51 LLIDD363 pKa = 4.2 KK364 pKa = 10.1 YY365 pKa = 10.96 FGPSLPAGAVQIEE378 pKa = 4.17 NDD380 pKa = 4.38 AFDD383 pKa = 3.49 QAPNIVRR390 pKa = 11.84 ADD392 pKa = 3.19 GGLWRR397 pKa = 11.84 KK398 pKa = 9.4 GRR400 pKa = 11.84 ASYY403 pKa = 10.51 LVEE406 pKa = 3.85 EE407 pKa = 4.71 EE408 pKa = 4.52 GRR410 pKa = 11.84 LGTVDD415 pKa = 3.73 LSYY418 pKa = 10.96 YY419 pKa = 10.85 APEE422 pKa = 4.01 TTNRR426 pKa = 11.84 GLPSTEE432 pKa = 3.8 PWFHH436 pKa = 6.72 FPPRR440 pKa = 11.84 SPTQLQNLEE449 pKa = 4.38 MYY451 pKa = 10.63 LKK453 pKa = 10.75 NNWGGEE459 pKa = 3.92 NTYY462 pKa = 9.75 YY463 pKa = 10.66 QPTGEE468 pKa = 4.26 GTFLIGDD475 pKa = 4.55 LVLQEE480 pKa = 3.69 AQYY483 pKa = 10.68 RR484 pKa = 11.84 RR485 pKa = 11.84 KK486 pKa = 9.65 NARR489 pKa = 11.84 AKK491 pKa = 10.21 RR492 pKa = 11.84 RR493 pKa = 11.84 PRR495 pKa = 11.84 DD496 pKa = 3.52 TGPGTRR502 pKa = 11.84 TKK504 pKa = 9.59 RR505 pKa = 11.84 TRR507 pKa = 11.84 RR508 pKa = 11.84 ARR510 pKa = 11.84 NN511 pKa = 3.05
Molecular weight: 54.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.902
IPC2_protein 4.863
IPC_protein 4.787
Toseland 4.66
ProMoST 4.876
Dawson 4.724
Bjellqvist 4.863
Wikipedia 4.558
Rodwell 4.635
Grimsley 4.571
Solomon 4.724
Lehninger 4.673
Nozaki 4.825
DTASelect 4.94
Thurlkill 4.635
EMBOSS 4.584
Sillero 4.902
Patrickios 4.228
IPC_peptide 4.724
IPC2_peptide 4.902
IPC2.peptide.svr19 4.84
Protein with the highest isoelectric point:
>tr|A0A1R3T5J8|A0A1R3T5J8_9POLY Late orf 2 OS=Pomona leaf-nosed bat associated polyomavirus OX=1885565 GN=LO2 PE=4 SV=1
MM1 pKa = 7.78 LLLQLLGLRR10 pKa = 11.84 GQLPRR15 pKa = 11.84 LLLPALNSLVQLEE28 pKa = 4.58 KK29 pKa = 11.22 LLGTLFRR36 pKa = 11.84 SLMEE40 pKa = 4.02 KK41 pKa = 10.1 RR42 pKa = 11.84 KK43 pKa = 9.91 QSSTPLGPRR52 pKa = 11.84 GAMLMRR58 pKa = 11.84 GLLMTRR64 pKa = 11.84 GTGKK68 pKa = 9.78 WNLRR72 pKa = 11.84 TWLLVVAADD81 pKa = 5.04 CPDD84 pKa = 3.77 LLLPAWQLGQEE95 pKa = 3.98 WQLPRR100 pKa = 11.84 GAWPGQLVRR109 pKa = 11.84 LLLLILRR116 pKa = 11.84 LVKK119 pKa = 10.4 PSLRR123 pKa = 11.84 ALKK126 pKa = 10.49
Molecular weight: 14.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.161
IPC2_protein 10.175
IPC_protein 11.696
Toseland 11.886
ProMoST 12.354
Dawson 11.886
Bjellqvist 11.871
Wikipedia 12.354
Rodwell 11.696
Grimsley 11.93
Solomon 12.369
Lehninger 12.266
Nozaki 11.886
DTASelect 11.871
Thurlkill 11.886
EMBOSS 12.384
Sillero 11.886
Patrickios 11.433
IPC_peptide 12.369
IPC2_peptide 11.345
IPC2.peptide.svr19 9.396
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1645
44
616
329.0
36.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.119 ± 2.208
1.337 ± 0.511
4.681 ± 0.798
6.261 ± 1.056
4.134 ± 1.108
8.693 ± 1.164
1.824 ± 0.688
3.04 ± 0.303
4.012 ± 1.096
11.003 ± 1.878
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.614 ± 0.327
3.647 ± 0.799
6.383 ± 0.739
4.377 ± 0.426
6.261 ± 1.119
7.234 ± 0.578
5.532 ± 0.975
5.775 ± 0.587
1.641 ± 0.353
2.432 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here