Pythium nunn virus 1
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5ZAM9|A0A2Z5ZAM9_9VIRU Putative capsid protein OS=Pythium nunn virus 1 OX=2083275 GN=CP PE=4 SV=1
MM1 pKa = 7.68 NYY3 pKa = 9.83 NARR6 pKa = 11.84 DD7 pKa = 3.43 VGEE10 pKa = 4.28 AVSNGQPPRR19 pKa = 11.84 RR20 pKa = 11.84 AGDD23 pKa = 3.64 NPNKK27 pKa = 10.42 AFAQRR32 pKa = 11.84 RR33 pKa = 11.84 QGNRR37 pKa = 11.84 PKK39 pKa = 10.83 VKK41 pKa = 9.1 NTPSGPKK48 pKa = 7.61 TTTEE52 pKa = 3.57 AFRR55 pKa = 11.84 WIEE58 pKa = 3.98 PLPEE62 pKa = 4.18 VTPMPAVLEE71 pKa = 4.65 TITEE75 pKa = 4.45 SIPAGSIEE83 pKa = 5.22 LNFEE87 pKa = 4.26 LPSTVASPFTQSVEE101 pKa = 4.28 SVLMRR106 pKa = 11.84 TDD108 pKa = 3.84 LPSASIYY115 pKa = 7.73 QARR118 pKa = 11.84 DD119 pKa = 3.06 KK120 pKa = 11.56 LEE122 pKa = 3.97 ALSYY126 pKa = 11.02 YY127 pKa = 9.79 KK128 pKa = 10.4 ACRR131 pKa = 11.84 QLYY134 pKa = 8.14 STLSDD139 pKa = 3.59 PQKK142 pKa = 11.05 AALQPLKK149 pKa = 10.65 AIYY152 pKa = 9.49 YY153 pKa = 8.49 DD154 pKa = 3.63 KK155 pKa = 11.42 SHH157 pKa = 6.98 IPNHH161 pKa = 5.51 MSAALSMIGNLEE173 pKa = 4.13 SKK175 pKa = 9.92 MGLIEE180 pKa = 4.3 VKK182 pKa = 10.14 HH183 pKa = 6.19 APVLFKK189 pKa = 10.55 RR190 pKa = 11.84 WLIAGLCIDD199 pKa = 4.9 PDD201 pKa = 3.96 TTYY204 pKa = 10.42 TAATVAPNSLVFKK217 pKa = 10.44 DD218 pKa = 4.29 VYY220 pKa = 11.01 SKK222 pKa = 11.7 ALIDD226 pKa = 3.61 EE227 pKa = 4.63 KK228 pKa = 11.39 ANEE231 pKa = 4.06 RR232 pKa = 11.84 LKK234 pKa = 10.62 EE235 pKa = 3.85 IHH237 pKa = 6.57 NEE239 pKa = 3.82 TFKK242 pKa = 11.04 IKK244 pKa = 10.7 VGTTDD249 pKa = 3.37 IEE251 pKa = 4.48 VSAPIPPPTDD261 pKa = 2.77 AGYY264 pKa = 11.4 NSIPDD269 pKa = 4.53 DD270 pKa = 3.93 YY271 pKa = 11.23 PHH273 pKa = 7.16 ADD275 pKa = 3.23 EE276 pKa = 5.08 MRR278 pKa = 11.84 DD279 pKa = 3.45 LVSIIQMATRR289 pKa = 11.84 DD290 pKa = 4.0 YY291 pKa = 11.28 VRR293 pKa = 11.84 SQPVPHH299 pKa = 6.79 GRR301 pKa = 11.84 DD302 pKa = 3.03 LDD304 pKa = 3.99 TALSHH309 pKa = 7.18 LGLQVAPAGYY319 pKa = 9.98 SDD321 pKa = 3.52 QDD323 pKa = 3.31 LRR325 pKa = 11.84 TAFEE329 pKa = 4.5 DD330 pKa = 4.74 AIEE333 pKa = 4.55 TYY335 pKa = 9.22 LTTEE339 pKa = 4.68 IIHH342 pKa = 6.01 IQTVFHH348 pKa = 6.66 LGEE351 pKa = 4.42 PPATEE356 pKa = 4.12 RR357 pKa = 11.84 GFASQLVASDD367 pKa = 4.07 DD368 pKa = 3.45 VTARR372 pKa = 11.84 FGLPLSDD379 pKa = 3.98 ADD381 pKa = 3.76 KK382 pKa = 11.48 ALGFMFNPRR391 pKa = 11.84 HH392 pKa = 5.53 SFEE395 pKa = 4.18 FNPRR399 pKa = 11.84 FVAYY403 pKa = 9.87 SRR405 pKa = 11.84 RR406 pKa = 11.84 AKK408 pKa = 9.56 RR409 pKa = 11.84 TTASQFASNDD419 pKa = 3.08 LRR421 pKa = 11.84 HH422 pKa = 6.6 IGTDD426 pKa = 3.0
Molecular weight: 47.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.717
IPC2_protein 5.626
IPC_protein 5.664
Toseland 6.109
ProMoST 5.881
Dawson 5.893
Bjellqvist 5.919
Wikipedia 5.893
Rodwell 5.881
Grimsley 6.3
Solomon 5.893
Lehninger 5.893
Nozaki 6.148
DTASelect 6.351
Thurlkill 6.338
EMBOSS 6.3
Sillero 6.249
Patrickios 4.279
IPC_peptide 5.919
IPC2_peptide 6.249
IPC2.peptide.svr19 6.235
Protein with the highest isoelectric point:
>tr|A0A2Z5ZAM9|A0A2Z5ZAM9_9VIRU Putative capsid protein OS=Pythium nunn virus 1 OX=2083275 GN=CP PE=4 SV=1
MM1 pKa = 6.44 TTKK4 pKa = 9.37 KK5 pKa = 8.77 TRR7 pKa = 11.84 AEE9 pKa = 3.85 VQEE12 pKa = 3.98 FLKK15 pKa = 10.36 TIAGKK20 pKa = 9.51 PGLRR24 pKa = 11.84 VVPDD28 pKa = 4.26 PNAYY32 pKa = 9.02 PYY34 pKa = 10.61 NVEE37 pKa = 4.08 EE38 pKa = 4.35 TRR40 pKa = 11.84 MNTDD44 pKa = 3.02 PFVRR48 pKa = 11.84 KK49 pKa = 9.74 SMKK52 pKa = 8.84 LWSRR56 pKa = 11.84 EE57 pKa = 3.69 TYY59 pKa = 8.36 EE60 pKa = 3.99 AQRR63 pKa = 11.84 GFTKK67 pKa = 10.58 RR68 pKa = 11.84 ATLSNSLYY76 pKa = 9.97 TLQKK80 pKa = 8.74 YY81 pKa = 9.89 ASPQRR86 pKa = 11.84 LKK88 pKa = 11.03 SQISHH93 pKa = 5.97 EE94 pKa = 4.25 FEE96 pKa = 4.22 SCIEE100 pKa = 4.01 SAIAYY105 pKa = 7.45 ARR107 pKa = 11.84 HH108 pKa = 5.34 VFIPQDD114 pKa = 3.05 KK115 pKa = 10.1 LLRR118 pKa = 11.84 YY119 pKa = 9.4 SLAHH123 pKa = 6.02 GTDD126 pKa = 3.0 NMNLDD131 pKa = 3.56 SAAGFSYY138 pKa = 10.75 PGMKK142 pKa = 9.65 KK143 pKa = 10.47 AEE145 pKa = 4.33 VVEE148 pKa = 4.17 EE149 pKa = 4.63 AYY151 pKa = 10.44 DD152 pKa = 3.5 VAAYY156 pKa = 8.71 LQHH159 pKa = 5.05 MTQRR163 pKa = 11.84 GKK165 pKa = 10.35 SVYY168 pKa = 10.01 IPPCKK173 pKa = 9.79 VALRR177 pKa = 11.84 GHH179 pKa = 7.15 LSSQEE184 pKa = 4.07 EE185 pKa = 4.33 QKK187 pKa = 10.86 SRR189 pKa = 11.84 PVWVYY194 pKa = 9.26 PVEE197 pKa = 4.22 VTILEE202 pKa = 4.51 SKK204 pKa = 9.14 WAIPFYY210 pKa = 10.78 EE211 pKa = 4.04 HH212 pKa = 7.6 LEE214 pKa = 4.29 RR215 pKa = 11.84 NVEE218 pKa = 4.34 TVHH221 pKa = 6.79 FGANAMPKK229 pKa = 10.37 LSQLLMGGLADD240 pKa = 3.61 HH241 pKa = 6.9 SEE243 pKa = 4.08 AAEE246 pKa = 4.08 ITLDD250 pKa = 3.13 WSNFDD255 pKa = 3.58 SSIPNWLINQAFDD268 pKa = 4.85 IIWEE272 pKa = 4.32 SFDD275 pKa = 3.26 NQYY278 pKa = 11.41 AYY280 pKa = 10.66 HH281 pKa = 6.83 EE282 pKa = 4.89 GEE284 pKa = 3.76 AVYY287 pKa = 10.28 GGEE290 pKa = 3.93 VMEE293 pKa = 4.95 RR294 pKa = 11.84 KK295 pKa = 9.8 NINLFRR301 pKa = 11.84 WIKK304 pKa = 10.37 EE305 pKa = 3.61 YY306 pKa = 10.75 FINTKK311 pKa = 10.1 IMLPSGDD318 pKa = 3.69 VVRR321 pKa = 11.84 KK322 pKa = 6.49 THH324 pKa = 7.05 GIPSGSFFTQAVGSIVNYY342 pKa = 10.35 IMVKK346 pKa = 10.57 ALDD349 pKa = 5.03 LYY351 pKa = 10.26 FSWGARR357 pKa = 11.84 RR358 pKa = 11.84 IRR360 pKa = 11.84 ILGDD364 pKa = 3.23 DD365 pKa = 3.98 SSFLIPLFSEE375 pKa = 4.81 KK376 pKa = 10.24 KK377 pKa = 10.03 CEE379 pKa = 3.98 AKK381 pKa = 10.5 AIQNAAWKK389 pKa = 10.49 AFGVTLKK396 pKa = 10.71 LPKK399 pKa = 10.21 LRR401 pKa = 11.84 ISTTQKK407 pKa = 8.99 QRR409 pKa = 11.84 KK410 pKa = 7.56 FLGYY414 pKa = 10.19 QITGHH419 pKa = 6.59 RR420 pKa = 11.84 LFRR423 pKa = 11.84 DD424 pKa = 3.21 DD425 pKa = 6.49 DD426 pKa = 3.98 EE427 pKa = 4.41 FLKK430 pKa = 11.06 LVLYY434 pKa = 8.8 PEE436 pKa = 4.93 RR437 pKa = 11.84 DD438 pKa = 3.55 VEE440 pKa = 4.31 TLEE443 pKa = 4.15 QSASRR448 pKa = 11.84 VLAYY452 pKa = 11.12 YY453 pKa = 9.8 MLGGVNDD460 pKa = 3.78 EE461 pKa = 4.85 VYY463 pKa = 10.98 CSFFWDD469 pKa = 3.5 YY470 pKa = 11.17 LGRR473 pKa = 11.84 YY474 pKa = 6.48 PAVIGRR480 pKa = 11.84 EE481 pKa = 3.97 LRR483 pKa = 11.84 LTRR486 pKa = 11.84 GMKK489 pKa = 10.34 RR490 pKa = 11.84 MFKK493 pKa = 10.02 FVLRR497 pKa = 11.84 QEE499 pKa = 4.21 VDD501 pKa = 3.39 VLRR504 pKa = 11.84 VPDD507 pKa = 4.2 FTKK510 pKa = 10.71 LDD512 pKa = 3.84 PTFVPYY518 pKa = 11.24 LMTLGDD524 pKa = 4.5 RR525 pKa = 11.84 PFSSNN530 pKa = 3.07
Molecular weight: 61.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.13
IPC2_protein 8.229
IPC_protein 8.141
Toseland 8.565
ProMoST 8.595
Dawson 8.975
Bjellqvist 8.873
Wikipedia 9.136
Rodwell 9.063
Grimsley 8.96
Solomon 9.048
Lehninger 9.004
Nozaki 8.873
DTASelect 8.785
Thurlkill 8.858
EMBOSS 9.092
Sillero 9.033
Patrickios 4.406
IPC_peptide 9.033
IPC2_peptide 7.556
IPC2.peptide.svr19 7.625
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
956
426
530
478.0
54.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.473 ± 1.267
0.628 ± 0.108
5.544 ± 0.703
6.276 ± 0.439
4.812 ± 0.561
5.021 ± 0.383
2.301 ± 0.192
5.335 ± 0.204
5.649 ± 0.651
8.577 ± 0.407
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.51 ± 0.272
3.766 ± 0.154
6.172 ± 1.236
3.661 ± 0.096
6.172 ± 0.207
7.218 ± 0.201
6.067 ± 0.826
6.172 ± 0.527
1.255 ± 0.537
4.393 ± 0.596
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here