Brettanomyces naardenensis (Yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta;

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5175 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A448YND6|A0A448YND6_BRENA Ribosome assembly protein 3 OS=Brettanomyces naardenensis OX=13370 GN=BRENAR_LOCUS3161 PE=3 SV=1
MM1 pKa = 7.65GLFDD5 pKa = 3.97KK6 pKa = 11.33AKK8 pKa = 10.7DD9 pKa = 3.51AVNNNDD15 pKa = 3.48FRR17 pKa = 11.84NKK19 pKa = 10.7AEE21 pKa = 3.99GAFNSFKK28 pKa = 10.84GGKK31 pKa = 9.02NNNSNDD37 pKa = 3.68DD38 pKa = 3.78QNDD41 pKa = 3.06NSYY44 pKa = 10.46GQSGSNDD51 pKa = 3.28NNDD54 pKa = 3.46SYY56 pKa = 11.86GGSSNQNDD64 pKa = 3.14NSYY67 pKa = 11.26GGNSNQNDD75 pKa = 3.09NSYY78 pKa = 11.28GGNSSNDD85 pKa = 2.82NSYY88 pKa = 11.37GGNSSNDD95 pKa = 2.83NSYY98 pKa = 11.27GGRR101 pKa = 11.84SNQNDD106 pKa = 3.44DD107 pKa = 3.94SYY109 pKa = 12.03GGRR112 pKa = 11.84SNQNDD117 pKa = 3.43DD118 pKa = 4.1SYY120 pKa = 12.03GGNSNQNDD128 pKa = 3.09NSYY131 pKa = 11.26GGNSNQNDD139 pKa = 3.6DD140 pKa = 5.17SYY142 pKa = 12.12GNDD145 pKa = 3.09NNRR148 pKa = 11.84DD149 pKa = 3.25NNYY152 pKa = 10.7

Molecular weight: 16.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A448YRI0|A0A448YRI0_BRENA DEKNAAC104647 OS=Brettanomyces naardenensis OX=13370 GN=BRENAR_LOCUS4235 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.42QKK12 pKa = 10.0LAKK15 pKa = 9.31HH16 pKa = 5.36QKK18 pKa = 8.05QNRR21 pKa = 11.84PLPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TNNKK34 pKa = 8.41IRR36 pKa = 11.84YY37 pKa = 5.84NAKK40 pKa = 7.26RR41 pKa = 11.84THH43 pKa = 4.58WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.76LHH50 pKa = 6.06LL51 pKa = 4.34

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5164

11

5175

2563765

33

4964

495.4

55.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.32 ± 0.031

1.2 ± 0.012

6.213 ± 0.028

7.021 ± 0.041

4.262 ± 0.022

6.008 ± 0.034

1.918 ± 0.012

5.762 ± 0.025

6.555 ± 0.035

9.513 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.115 ± 0.012

4.443 ± 0.018

4.581 ± 0.027

3.807 ± 0.031

5.252 ± 0.025

8.875 ± 0.042

5.433 ± 0.029

6.314 ± 0.025

1.013 ± 0.011

3.395 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski