Amino acid dipepetide frequency for Clostridium phage CPD7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
1.046AlaCys: 1.046 ± 0.294
2.79AlaAsp: 2.79 ± 0.718
2.441AlaGlu: 2.441 ± 0.795
1.569AlaPhe: 1.569 ± 0.497
5.405AlaGly: 5.405 ± 2.829
1.221AlaHis: 1.221 ± 0.472
2.964AlaIle: 2.964 ± 0.68
3.487AlaLys: 3.487 ± 0.593
2.964AlaLeu: 2.964 ± 0.531
0.872AlaMet: 0.872 ± 0.468
2.616AlaAsn: 2.616 ± 0.751
1.395AlaPro: 1.395 ± 0.404
1.569AlaGln: 1.569 ± 0.711
0.697AlaArg: 0.697 ± 0.256
1.221AlaSer: 1.221 ± 0.321
1.569AlaThr: 1.569 ± 0.464
1.744AlaVal: 1.744 ± 0.54
0.0AlaTrp: 0.0 ± 0.0
2.441AlaTyr: 2.441 ± 0.537
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.349CysCys: 0.349 ± 0.193
0.697CysAsp: 0.697 ± 0.294
1.221CysGlu: 1.221 ± 0.414
1.569CysPhe: 1.569 ± 0.679
1.046CysGly: 1.046 ± 0.469
0.349CysHis: 0.349 ± 0.263
0.523CysIle: 0.523 ± 0.362
1.046CysLys: 1.046 ± 0.428
1.046CysLeu: 1.046 ± 0.507
0.349CysMet: 0.349 ± 0.243
0.872CysAsn: 0.872 ± 0.441
0.349CysPro: 0.349 ± 0.261
0.349CysGln: 0.349 ± 0.282
0.174CysArg: 0.174 ± 0.245
0.872CysSer: 0.872 ± 0.482
1.046CysThr: 1.046 ± 0.326
0.523CysVal: 0.523 ± 0.284
0.0CysTrp: 0.0 ± 0.0
1.395CysTyr: 1.395 ± 0.486
0.0CysXaa: 0.0 ± 0.0
Asp
1.744AspAla: 1.744 ± 0.625
1.046AspCys: 1.046 ± 0.43
2.616AspAsp: 2.616 ± 0.531
4.185AspGlu: 4.185 ± 0.917
3.662AspPhe: 3.662 ± 0.723
6.277AspGly: 6.277 ± 1.316
0.872AspHis: 0.872 ± 0.352
5.057AspIle: 5.057 ± 0.786
5.754AspLys: 5.754 ± 1.156
5.929AspLeu: 5.929 ± 1.017
0.872AspMet: 0.872 ± 0.413
4.359AspAsn: 4.359 ± 0.857
1.046AspPro: 1.046 ± 0.46
0.872AspGln: 0.872 ± 0.392
3.662AspArg: 3.662 ± 0.686
3.487AspSer: 3.487 ± 0.782
2.616AspThr: 2.616 ± 0.572
2.79AspVal: 2.79 ± 0.763
1.395AspTrp: 1.395 ± 0.964
4.185AspTyr: 4.185 ± 0.913
0.0AspXaa: 0.0 ± 0.0
Glu
1.918GluAla: 1.918 ± 0.594
2.267GluCys: 2.267 ± 0.676
3.836GluAsp: 3.836 ± 0.547
5.754GluGlu: 5.754 ± 1.466
2.267GluPhe: 2.267 ± 0.793
5.057GluGly: 5.057 ± 0.981
1.046GluHis: 1.046 ± 0.402
8.195GluIle: 8.195 ± 1.102
8.37GluLys: 8.37 ± 1.376
10.462GluLeu: 10.462 ± 2.001
3.139GluMet: 3.139 ± 0.885
8.021GluAsn: 8.021 ± 1.458
2.092GluPro: 2.092 ± 0.601
3.487GluGln: 3.487 ± 0.943
4.185GluArg: 4.185 ± 0.788
2.79GluSer: 2.79 ± 0.665
3.487GluThr: 3.487 ± 0.724
4.359GluVal: 4.359 ± 0.681
0.523GluTrp: 0.523 ± 0.295
3.836GluTyr: 3.836 ± 0.737
0.0GluXaa: 0.0 ± 0.0
Phe
2.616PheAla: 2.616 ± 0.75
0.697PheCys: 0.697 ± 0.428
3.313PheAsp: 3.313 ± 0.93
3.662PheGlu: 3.662 ± 0.835
1.395PhePhe: 1.395 ± 0.586
2.79PheGly: 2.79 ± 0.489
0.697PheHis: 0.697 ± 0.332
4.708PheIle: 4.708 ± 1.355
2.964PheLys: 2.964 ± 0.522
3.662PheLeu: 3.662 ± 0.965
1.395PheMet: 1.395 ± 0.442
2.616PheAsn: 2.616 ± 0.588
1.569PhePro: 1.569 ± 0.647
1.744PheGln: 1.744 ± 0.432
2.441PheArg: 2.441 ± 0.684
2.092PheSer: 2.092 ± 0.576
2.964PheThr: 2.964 ± 0.673
2.441PheVal: 2.441 ± 0.64
0.174PheTrp: 0.174 ± 0.198
2.092PheTyr: 2.092 ± 0.583
0.0PheXaa: 0.0 ± 0.0
Gly
2.964GlyAla: 2.964 ± 0.741
1.221GlyCys: 1.221 ± 0.595
4.708GlyAsp: 4.708 ± 1.06
5.929GlyGlu: 5.929 ± 1.188
2.616GlyPhe: 2.616 ± 0.611
4.534GlyGly: 4.534 ± 0.927
0.697GlyHis: 0.697 ± 0.538
4.708GlyIle: 4.708 ± 0.874
5.057GlyLys: 5.057 ± 1.032
6.452GlyLeu: 6.452 ± 0.973
0.697GlyMet: 0.697 ± 0.31
3.487GlyAsn: 3.487 ± 0.72
5.754GlyPro: 5.754 ± 3.465
1.046GlyGln: 1.046 ± 0.38
2.267GlyArg: 2.267 ± 0.644
3.139GlySer: 3.139 ± 0.604
2.616GlyThr: 2.616 ± 0.839
7.149GlyVal: 7.149 ± 1.111
0.349GlyTrp: 0.349 ± 0.22
3.313GlyTyr: 3.313 ± 0.626
0.0GlyXaa: 0.0 ± 0.0
His
0.174HisAla: 0.174 ± 0.212
0.174HisCys: 0.174 ± 0.176
0.523HisAsp: 0.523 ± 0.334
1.221HisGlu: 1.221 ± 0.5
1.046HisPhe: 1.046 ± 0.344
1.395HisGly: 1.395 ± 0.684
0.349HisHis: 0.349 ± 0.281
1.569HisIle: 1.569 ± 0.552
0.523HisLys: 0.523 ± 0.296
1.395HisLeu: 1.395 ± 0.552
0.174HisMet: 0.174 ± 0.212
1.569HisAsn: 1.569 ± 0.594
0.174HisPro: 0.174 ± 0.158
0.0HisGln: 0.0 ± 0.0
0.174HisArg: 0.174 ± 0.216
1.395HisSer: 1.395 ± 0.464
0.523HisThr: 0.523 ± 0.452
0.872HisVal: 0.872 ± 0.384
0.349HisTrp: 0.349 ± 0.243
1.046HisTyr: 1.046 ± 0.445
0.0HisXaa: 0.0 ± 0.0
Ile
1.918IleAla: 1.918 ± 0.511
0.697IleCys: 0.697 ± 0.403
6.277IleAsp: 6.277 ± 1.034
7.149IleGlu: 7.149 ± 1.317
3.662IlePhe: 3.662 ± 0.914
5.231IleGly: 5.231 ± 1.69
0.697IleHis: 0.697 ± 0.298
4.708IleIle: 4.708 ± 1.217
7.672IleLys: 7.672 ± 1.349
4.01IleLeu: 4.01 ± 0.728
2.092IleMet: 2.092 ± 0.495
7.498IleAsn: 7.498 ± 1.454
2.441IlePro: 2.441 ± 0.68
2.79IleGln: 2.79 ± 0.776
2.964IleArg: 2.964 ± 0.796
4.534IleSer: 4.534 ± 0.839
3.836IleThr: 3.836 ± 0.711
3.662IleVal: 3.662 ± 0.81
0.872IleTrp: 0.872 ± 0.273
3.662IleTyr: 3.662 ± 0.965
0.0IleXaa: 0.0 ± 0.0
Lys
4.708LysAla: 4.708 ± 0.979
1.046LysCys: 1.046 ± 0.529
5.231LysAsp: 5.231 ± 1.108
10.636LysGlu: 10.636 ± 1.764
2.964LysPhe: 2.964 ± 0.573
7.149LysGly: 7.149 ± 1.078
1.395LysHis: 1.395 ± 0.526
8.195LysIle: 8.195 ± 1.093
6.8LysLys: 6.8 ± 1.229
8.893LysLeu: 8.893 ± 1.257
2.092LysMet: 2.092 ± 0.641
7.847LysAsn: 7.847 ± 1.127
1.569LysPro: 1.569 ± 0.442
2.441LysGln: 2.441 ± 0.75
3.139LysArg: 3.139 ± 0.9
5.231LysSer: 5.231 ± 0.885
3.313LysThr: 3.313 ± 0.704
4.708LysVal: 4.708 ± 0.989
1.046LysTrp: 1.046 ± 0.401
3.836LysTyr: 3.836 ± 0.824
0.0LysXaa: 0.0 ± 0.0
Leu
2.441LeuAla: 2.441 ± 0.594
0.697LeuCys: 0.697 ± 0.276
5.057LeuAsp: 5.057 ± 1.078
7.672LeuGlu: 7.672 ± 1.346
3.662LeuPhe: 3.662 ± 1.057
5.754LeuGly: 5.754 ± 1.206
1.569LeuHis: 1.569 ± 0.491
5.231LeuIle: 5.231 ± 1.063
10.288LeuLys: 10.288 ± 1.774
6.626LeuLeu: 6.626 ± 1.153
1.918LeuMet: 1.918 ± 0.614
6.975LeuAsn: 6.975 ± 1.465
2.092LeuPro: 2.092 ± 0.559
3.487LeuGln: 3.487 ± 0.606
2.964LeuArg: 2.964 ± 0.739
4.185LeuSer: 4.185 ± 0.675
3.662LeuThr: 3.662 ± 0.797
2.964LeuVal: 2.964 ± 0.57
0.523LeuTrp: 0.523 ± 0.237
4.359LeuTyr: 4.359 ± 0.798
0.0LeuXaa: 0.0 ± 0.0
Met
1.221MetAla: 1.221 ± 0.47
0.174MetCys: 0.174 ± 0.161
1.744MetAsp: 1.744 ± 0.451
1.569MetGlu: 1.569 ± 0.623
0.872MetPhe: 0.872 ± 0.462
0.872MetGly: 0.872 ± 0.439
0.0MetHis: 0.0 ± 0.0
1.569MetIle: 1.569 ± 0.459
2.79MetLys: 2.79 ± 0.785
1.221MetLeu: 1.221 ± 0.413
1.046MetMet: 1.046 ± 0.472
1.395MetAsn: 1.395 ± 0.492
0.523MetPro: 0.523 ± 0.321
0.523MetGln: 0.523 ± 0.276
0.697MetArg: 0.697 ± 0.333
1.395MetSer: 1.395 ± 0.449
1.395MetThr: 1.395 ± 0.399
1.395MetVal: 1.395 ± 0.554
0.174MetTrp: 0.174 ± 0.161
1.395MetTyr: 1.395 ± 0.414
0.0MetXaa: 0.0 ± 0.0
Asn
2.616AsnAla: 2.616 ± 0.704
0.523AsnCys: 0.523 ± 0.358
5.231AsnAsp: 5.231 ± 1.342
5.405AsnGlu: 5.405 ± 0.96
3.139AsnPhe: 3.139 ± 0.846
5.057AsnGly: 5.057 ± 1.098
0.872AsnHis: 0.872 ± 0.385
7.149AsnIle: 7.149 ± 1.819
8.195AsnLys: 8.195 ± 1.111
4.708AsnLeu: 4.708 ± 0.767
1.221AsnMet: 1.221 ± 0.368
5.929AsnAsn: 5.929 ± 1.09
2.267AsnPro: 2.267 ± 0.568
2.441AsnGln: 2.441 ± 0.625
4.01AsnArg: 4.01 ± 1.007
6.103AsnSer: 6.103 ± 1.172
2.79AsnThr: 2.79 ± 0.922
4.708AsnVal: 4.708 ± 0.893
1.046AsnTrp: 1.046 ± 0.317
4.708AsnTyr: 4.708 ± 0.952
0.0AsnXaa: 0.0 ± 0.0
Pro
3.836ProAla: 3.836 ± 2.299
0.174ProCys: 0.174 ± 0.212
1.395ProAsp: 1.395 ± 0.279
2.79ProGlu: 2.79 ± 0.792
0.697ProPhe: 0.697 ± 0.392
0.174ProGly: 0.174 ± 0.161
0.523ProHis: 0.523 ± 0.3
2.441ProIle: 2.441 ± 0.751
1.918ProLys: 1.918 ± 0.753
1.744ProLeu: 1.744 ± 0.395
0.349ProMet: 0.349 ± 0.211
1.569ProAsn: 1.569 ± 0.53
0.697ProPro: 0.697 ± 0.393
1.744ProGln: 1.744 ± 0.5
1.046ProArg: 1.046 ± 0.454
2.441ProSer: 2.441 ± 0.715
1.046ProThr: 1.046 ± 0.516
2.616ProVal: 2.616 ± 0.878
0.0ProTrp: 0.0 ± 0.0
2.79ProTyr: 2.79 ± 0.615
0.0ProXaa: 0.0 ± 0.0
Gln
1.569GlnAla: 1.569 ± 0.604
0.349GlnCys: 0.349 ± 0.225
1.569GlnAsp: 1.569 ± 0.527
3.487GlnGlu: 3.487 ± 0.824
1.221GlnPhe: 1.221 ± 0.333
2.964GlnGly: 2.964 ± 0.746
0.697GlnHis: 0.697 ± 0.416
1.221GlnIle: 1.221 ± 0.569
1.918GlnLys: 1.918 ± 0.698
2.616GlnLeu: 2.616 ± 0.745
0.523GlnMet: 0.523 ± 0.294
2.79GlnAsn: 2.79 ± 0.789
0.697GlnPro: 0.697 ± 0.367
2.441GlnGln: 2.441 ± 0.588
1.569GlnArg: 1.569 ± 0.529
1.744GlnSer: 1.744 ± 0.522
1.395GlnThr: 1.395 ± 0.437
1.046GlnVal: 1.046 ± 0.596
0.872GlnTrp: 0.872 ± 0.386
1.395GlnTyr: 1.395 ± 0.471
0.0GlnXaa: 0.0 ± 0.0
Arg
1.569ArgAla: 1.569 ± 0.532
0.697ArgCys: 0.697 ± 0.327
2.092ArgAsp: 2.092 ± 0.653
4.534ArgGlu: 4.534 ± 0.923
2.79ArgPhe: 2.79 ± 0.72
2.79ArgGly: 2.79 ± 0.986
0.174ArgHis: 0.174 ± 0.203
3.139ArgIle: 3.139 ± 0.973
3.487ArgLys: 3.487 ± 0.857
3.662ArgLeu: 3.662 ± 0.78
1.046ArgMet: 1.046 ± 0.394
2.267ArgAsn: 2.267 ± 0.679
1.221ArgPro: 1.221 ± 0.431
0.523ArgGln: 0.523 ± 0.326
1.569ArgArg: 1.569 ± 0.362
1.395ArgSer: 1.395 ± 0.517
0.872ArgThr: 0.872 ± 0.416
2.79ArgVal: 2.79 ± 0.721
0.872ArgTrp: 0.872 ± 0.333
1.569ArgTyr: 1.569 ± 0.527
0.0ArgXaa: 0.0 ± 0.0
Ser
2.79SerAla: 2.79 ± 0.832
0.872SerCys: 0.872 ± 0.446
3.836SerAsp: 3.836 ± 0.985
4.534SerGlu: 4.534 ± 0.804
3.313SerPhe: 3.313 ± 0.975
2.441SerGly: 2.441 ± 0.789
0.697SerHis: 0.697 ± 0.395
3.662SerIle: 3.662 ± 1.042
4.882SerLys: 4.882 ± 1.152
4.359SerLeu: 4.359 ± 0.868
0.697SerMet: 0.697 ± 0.264
5.231SerAsn: 5.231 ± 0.946
0.697SerPro: 0.697 ± 0.473
1.569SerGln: 1.569 ± 0.469
1.395SerArg: 1.395 ± 0.552
2.616SerSer: 2.616 ± 0.899
1.918SerThr: 1.918 ± 0.698
2.964SerVal: 2.964 ± 0.705
0.349SerTrp: 0.349 ± 0.267
2.964SerTyr: 2.964 ± 0.793
0.0SerXaa: 0.0 ± 0.0
Thr
0.697ThrAla: 0.697 ± 0.265
0.697ThrCys: 0.697 ± 0.405
2.616ThrAsp: 2.616 ± 0.516
4.359ThrGlu: 4.359 ± 1.124
2.267ThrPhe: 2.267 ± 0.858
3.487ThrGly: 3.487 ± 0.818
0.349ThrHis: 0.349 ± 0.193
2.616ThrIle: 2.616 ± 0.551
4.01ThrLys: 4.01 ± 0.796
4.534ThrLeu: 4.534 ± 0.85
0.697ThrMet: 0.697 ± 0.361
3.836ThrAsn: 3.836 ± 1.034
1.744ThrPro: 1.744 ± 0.465
1.395ThrGln: 1.395 ± 0.455
1.918ThrArg: 1.918 ± 0.744
2.092ThrSer: 2.092 ± 0.502
4.185ThrThr: 4.185 ± 1.278
2.267ThrVal: 2.267 ± 0.454
0.697ThrTrp: 0.697 ± 0.343
1.918ThrTyr: 1.918 ± 0.489
0.0ThrXaa: 0.0 ± 0.0
Val
2.267ValAla: 2.267 ± 0.701
0.174ValCys: 0.174 ± 0.216
4.708ValAsp: 4.708 ± 0.655
3.662ValGlu: 3.662 ± 0.772
3.662ValPhe: 3.662 ± 1.12
2.092ValGly: 2.092 ± 0.757
1.046ValHis: 1.046 ± 0.438
4.882ValIle: 4.882 ± 0.713
6.8ValLys: 6.8 ± 1.016
3.836ValLeu: 3.836 ± 0.753
1.395ValMet: 1.395 ± 0.487
5.58ValAsn: 5.58 ± 0.738
1.046ValPro: 1.046 ± 0.565
1.221ValGln: 1.221 ± 0.611
2.092ValArg: 2.092 ± 0.459
2.092ValSer: 2.092 ± 0.706
3.487ValThr: 3.487 ± 0.936
2.616ValVal: 2.616 ± 1.074
0.349ValTrp: 0.349 ± 0.258
2.441ValTyr: 2.441 ± 0.572
0.0ValXaa: 0.0 ± 0.0
Trp
0.174TrpAla: 0.174 ± 0.157
0.0TrpCys: 0.0 ± 0.0
1.395TrpAsp: 1.395 ± 0.63
1.395TrpGlu: 1.395 ± 0.532
0.697TrpPhe: 0.697 ± 0.415
0.872TrpGly: 0.872 ± 0.322
0.174TrpHis: 0.174 ± 0.157
0.872TrpIle: 0.872 ± 0.427
0.174TrpLys: 0.174 ± 0.14
1.046TrpLeu: 1.046 ± 0.342
0.174TrpMet: 0.174 ± 0.177
0.174TrpAsn: 0.174 ± 0.176
0.0TrpPro: 0.0 ± 0.0
0.523TrpGln: 0.523 ± 0.23
0.523TrpArg: 0.523 ± 0.301
0.174TrpSer: 0.174 ± 0.212
0.523TrpThr: 0.523 ± 0.268
0.872TrpVal: 0.872 ± 0.477
0.0TrpTrp: 0.0 ± 0.0
0.697TrpTyr: 0.697 ± 0.479
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.79TyrAla: 2.79 ± 0.741
0.872TyrCys: 0.872 ± 0.362
2.79TyrAsp: 2.79 ± 0.696
3.662TyrGlu: 3.662 ± 1.034
2.964TyrPhe: 2.964 ± 0.749
3.313TyrGly: 3.313 ± 0.803
1.221TyrHis: 1.221 ± 0.386
2.964TyrIle: 2.964 ± 0.696
6.103TyrLys: 6.103 ± 0.979
2.964TyrLeu: 2.964 ± 0.592
1.046TyrMet: 1.046 ± 0.346
3.487TyrAsn: 3.487 ± 1.208
2.616TyrPro: 2.616 ± 0.751
1.918TyrGln: 1.918 ± 0.667
1.569TyrArg: 1.569 ± 0.429
2.79TyrSer: 2.79 ± 0.65
3.139TyrThr: 3.139 ± 0.952
2.964TyrVal: 2.964 ± 0.588
0.872TyrTrp: 0.872 ± 0.5
5.231TyrTyr: 5.231 ± 1.089
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 25 proteins (5736 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski