Ralstonia phage RSS30

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S6C3K7|S6C3K7_9VIRU Uncharacterized protein OS=Ralstonia phage RSS30 OX=1352227 PE=4 SV=1
MM1 pKa = 7.42LAIDD5 pKa = 4.08FWLWAGFLLPVLPAVIIFRR24 pKa = 11.84GLL26 pKa = 3.04

Molecular weight: 2.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S6B968|S6B968_9VIRU Uncharacterized protein OS=Ralstonia phage RSS30 OX=1352227 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84IEE4 pKa = 4.92KK5 pKa = 10.63YY6 pKa = 10.31LDD8 pKa = 3.22QAIEE12 pKa = 3.97RR13 pKa = 11.84HH14 pKa = 4.95GLKK17 pKa = 10.07NDD19 pKa = 3.29SKK21 pKa = 9.94LAEE24 pKa = 4.14MLGVVQSAVSHH35 pKa = 5.58YY36 pKa = 8.54RR37 pKa = 11.84TGRR40 pKa = 11.84RR41 pKa = 11.84TADD44 pKa = 3.23NEE46 pKa = 4.08VCLRR50 pKa = 11.84LAQLLEE56 pKa = 4.28MEE58 pKa = 4.78NPLPIIMAADD68 pKa = 3.4MDD70 pKa = 4.05RR71 pKa = 11.84AEE73 pKa = 4.39RR74 pKa = 11.84AGQHH78 pKa = 5.54SLWEE82 pKa = 4.24VFSTRR87 pKa = 11.84MAASNATAALLLVLVASATNFVAPSPAKK115 pKa = 10.1AAPLSHH121 pKa = 6.0STAQRR126 pKa = 11.84FVLCKK131 pKa = 8.49IARR134 pKa = 11.84RR135 pKa = 11.84LRR137 pKa = 11.84EE138 pKa = 3.81RR139 pKa = 11.84LQQALRR145 pKa = 11.84AVQTSPKK152 pKa = 9.14TAPCC156 pKa = 4.24

Molecular weight: 17.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

250

26

156

83.3

9.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.8 ± 1.165

2.4 ± 0.856

4.8 ± 1.565

4.0 ± 1.619

2.4 ± 2.206

4.0 ± 1.352

3.2 ± 1.141

4.4 ± 1.785

2.8 ± 0.996

13.6 ± 2.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.0 ± 0.159

2.8 ± 0.685

4.4 ± 0.86

5.6 ± 1.607

9.6 ± 1.448

5.2 ± 1.788

3.2 ± 1.7

6.0 ± 0.655

1.6 ± 1.508

1.2 ± 0.288
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski