Amino acid dipepetide frequency for Siphonobacter aquaeclarae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.325AlaAla: 8.325 ± 0.091	0.743AlaCys: 0.743 ± 0.024	4.934AlaAsp: 4.934 ± 0.055	5.107AlaGlu: 5.107 ± 0.06	4.055AlaPhe: 4.055 ± 0.044	7.756AlaGly: 7.756 ± 0.071	1.376AlaHis: 1.376 ± 0.027	4.52AlaIle: 4.52 ± 0.064	3.67AlaLys: 3.67 ± 0.051	8.108AlaLeu: 8.108 ± 0.088	1.704AlaMet: 1.704 ± 0.033	3.189AlaAsn: 3.189 ± 0.052	3.26AlaPro: 3.26 ± 0.047	3.176AlaGln: 3.176 ± 0.047	4.483AlaArg: 4.483 ± 0.055	5.26AlaSer: 5.26 ± 0.058	4.659AlaThr: 4.659 ± 0.063	6.017AlaVal: 6.017 ± 0.072	1.202AlaTrp: 1.202 ± 0.027	3.078AlaTyr: 3.078 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.457CysAla: 0.457 ± 0.016	0.115CysCys: 0.115 ± 0.008	0.362CysAsp: 0.362 ± 0.014	0.366CysGlu: 0.366 ± 0.016	0.396CysPhe: 0.396 ± 0.013	0.605CysGly: 0.605 ± 0.022	0.205CysHis: 0.205 ± 0.01	0.402CysIle: 0.402 ± 0.016	0.259CysLys: 0.259 ± 0.013	0.875CysLeu: 0.875 ± 0.023	0.134CysMet: 0.134 ± 0.009	0.237CysAsn: 0.237 ± 0.014	0.335CysPro: 0.335 ± 0.015	0.279CysGln: 0.279 ± 0.012	0.49CysArg: 0.49 ± 0.017	0.445CysSer: 0.445 ± 0.017	0.414CysThr: 0.414 ± 0.016	0.452CysVal: 0.452 ± 0.015	0.121CysTrp: 0.121 ± 0.008	0.294CysTyr: 0.294 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	4.482AspAla: 4.482 ± 0.048	0.318AspCys: 0.318 ± 0.012	2.398AspAsp: 2.398 ± 0.043	3.152AspGlu: 3.152 ± 0.051	2.828AspPhe: 2.828 ± 0.043	4.467AspGly: 4.467 ± 0.065	0.941AspHis: 0.941 ± 0.021	3.056AspIle: 3.056 ± 0.034	2.568AspLys: 2.568 ± 0.044	5.237AspLeu: 5.237 ± 0.057	0.966AspMet: 0.966 ± 0.023	2.033AspAsn: 2.033 ± 0.037	2.654AspPro: 2.654 ± 0.04	1.817AspGln: 1.817 ± 0.033	3.162AspArg: 3.162 ± 0.047	2.707AspSer: 2.707 ± 0.039	2.579AspThr: 2.579 ± 0.04	3.509AspVal: 3.509 ± 0.042	0.973AspTrp: 0.973 ± 0.022	2.333AspTyr: 2.333 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	5.228GluAla: 5.228 ± 0.061	0.291GluCys: 0.291 ± 0.012	2.662GluAsp: 2.662 ± 0.04	3.972GluGlu: 3.972 ± 0.061	2.111GluPhe: 2.111 ± 0.036	3.784GluGly: 3.784 ± 0.045	1.011GluHis: 1.011 ± 0.027	3.61GluIle: 3.61 ± 0.048	4.167GluLys: 4.167 ± 0.06	5.54GluLeu: 5.54 ± 0.067	1.372GluMet: 1.372 ± 0.03	2.674GluAsn: 2.674 ± 0.043	2.05GluPro: 2.05 ± 0.037	2.321GluGln: 2.321 ± 0.04	3.161GluArg: 3.161 ± 0.048	2.948GluSer: 2.948 ± 0.044	3.351GluThr: 3.351 ± 0.045	3.983GluVal: 3.983 ± 0.055	0.853GluTrp: 0.853 ± 0.023	1.954GluTyr: 1.954 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	3.598PheAla: 3.598 ± 0.053	0.44PheCys: 0.44 ± 0.016	2.81PheAsp: 2.81 ± 0.044	2.505PheGlu: 2.505 ± 0.037	2.576PhePhe: 2.576 ± 0.041	3.738PheGly: 3.738 ± 0.046	0.935PheHis: 0.935 ± 0.028	2.179PheIle: 2.179 ± 0.039	1.727PheLys: 1.727 ± 0.037	4.929PheLeu: 4.929 ± 0.06	0.873PheMet: 0.873 ± 0.024	1.994PheAsn: 1.994 ± 0.035	2.117PhePro: 2.117 ± 0.032	1.789PheGln: 1.789 ± 0.032	3.352PheArg: 3.352 ± 0.041	3.628PheSer: 3.628 ± 0.041	2.854PheThr: 2.854 ± 0.044	3.189PheVal: 3.189 ± 0.046	0.735PheTrp: 0.735 ± 0.021	1.787PheTyr: 1.787 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	5.55GlyAla: 5.55 ± 0.071	0.662GlyCys: 0.662 ± 0.019	3.678GlyAsp: 3.678 ± 0.048	3.849GlyGlu: 3.849 ± 0.048	3.769GlyPhe: 3.769 ± 0.049	5.81GlyGly: 5.81 ± 0.073	1.341GlyHis: 1.341 ± 0.03	5.085GlyIle: 5.085 ± 0.058	5.076GlyLys: 5.076 ± 0.063	7.347GlyLeu: 7.347 ± 0.073	1.817GlyMet: 1.817 ± 0.035	3.496GlyAsn: 3.496 ± 0.054	2.253GlyPro: 2.253 ± 0.05	2.888GlyGln: 2.888 ± 0.046	4.011GlyArg: 4.011 ± 0.048	4.878GlySer: 4.878 ± 0.066	5.207GlyThr: 5.207 ± 0.076	5.223GlyVal: 5.223 ± 0.06	1.365GlyTrp: 1.365 ± 0.029	3.409GlyTyr: 3.409 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.362HisAla: 1.362 ± 0.03	0.174HisCys: 0.174 ± 0.009	0.861HisAsp: 0.861 ± 0.023	0.943HisGlu: 0.943 ± 0.027	1.154HisPhe: 1.154 ± 0.025	1.217HisGly: 1.217 ± 0.026	0.524HisHis: 0.524 ± 0.018	1.013HisIle: 1.013 ± 0.026	0.689HisLys: 0.689 ± 0.022	1.99HisLeu: 1.99 ± 0.033	0.323HisMet: 0.323 ± 0.013	0.668HisAsn: 0.668 ± 0.018	1.238HisPro: 1.238 ± 0.029	0.716HisGln: 0.716 ± 0.021	1.133HisArg: 1.133 ± 0.026	1.021HisSer: 1.021 ± 0.023	1.158HisThr: 1.158 ± 0.026	1.17HisVal: 1.17 ± 0.025	0.327HisTrp: 0.327 ± 0.013	0.841HisTyr: 0.841 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	4.836IleAla: 4.836 ± 0.057	0.503IleCys: 0.503 ± 0.018	3.062IleAsp: 3.062 ± 0.046	3.045IleGlu: 3.045 ± 0.04	2.165IlePhe: 2.165 ± 0.04	4.43IleGly: 4.43 ± 0.058	1.122IleHis: 1.122 ± 0.022	2.515IleIle: 2.515 ± 0.044	2.078IleLys: 2.078 ± 0.04	5.316IleLeu: 5.316 ± 0.061	0.763IleMet: 0.763 ± 0.021	2.197IleAsn: 2.197 ± 0.037	3.104IlePro: 3.104 ± 0.038	2.201IleGln: 2.201 ± 0.035	4.307IleArg: 4.307 ± 0.052	3.773IleSer: 3.773 ± 0.051	3.239IleThr: 3.239 ± 0.045	3.543IleVal: 3.543 ± 0.052	0.669IleTrp: 0.669 ± 0.018	1.77IleTyr: 1.77 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.655LysAla: 4.655 ± 0.061	0.193LysCys: 0.193 ± 0.011	2.934LysAsp: 2.934 ± 0.048	3.362LysGlu: 3.362 ± 0.056	1.654LysPhe: 1.654 ± 0.031	3.985LysGly: 3.985 ± 0.053	0.841LysHis: 0.841 ± 0.024	2.973LysIle: 2.973 ± 0.042	3.523LysLys: 3.523 ± 0.059	4.392LysLeu: 4.392 ± 0.056	1.252LysMet: 1.252 ± 0.028	2.482LysAsn: 2.482 ± 0.043	2.516LysPro: 2.516 ± 0.041	1.87LysGln: 1.87 ± 0.033	2.466LysArg: 2.466 ± 0.043	2.776LysSer: 2.776 ± 0.044	3.274LysThr: 3.274 ± 0.048	3.582LysVal: 3.582 ± 0.049	0.703LysTrp: 0.703 ± 0.022	1.93LysTyr: 1.93 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	8.723LeuAla: 8.723 ± 0.078	0.794LeuCys: 0.794 ± 0.02	4.852LeuAsp: 4.852 ± 0.056	5.245LeuGlu: 5.245 ± 0.067	4.977LeuPhe: 4.977 ± 0.062	6.63LeuGly: 6.63 ± 0.064	1.914LeuHis: 1.914 ± 0.033	5.333LeuIle: 5.333 ± 0.069	5.475LeuLys: 5.475 ± 0.06	11.136LeuLeu: 11.136 ± 0.118	1.952LeuMet: 1.952 ± 0.037	4.23LeuAsn: 4.23 ± 0.055	5.243LeuPro: 5.243 ± 0.062	3.503LeuGln: 3.503 ± 0.051	5.861LeuArg: 5.861 ± 0.063	7.341LeuSer: 7.341 ± 0.066	6.754LeuThr: 6.754 ± 0.061	6.456LeuVal: 6.456 ± 0.064	1.22LeuTrp: 1.22 ± 0.026	3.313LeuTyr: 3.313 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	1.76MetAla: 1.76 ± 0.038	0.1MetCys: 0.1 ± 0.008	0.978MetAsp: 0.978 ± 0.025	1.146MetGlu: 1.146 ± 0.025	0.594MetPhe: 0.594 ± 0.019	1.425MetGly: 1.425 ± 0.031	0.367MetHis: 0.367 ± 0.015	1.06MetIle: 1.06 ± 0.028	1.682MetLys: 1.682 ± 0.032	1.891MetLeu: 1.891 ± 0.036	0.459MetMet: 0.459 ± 0.019	0.989MetAsn: 0.989 ± 0.025	1.056MetPro: 1.056 ± 0.023	0.764MetGln: 0.764 ± 0.021	1.071MetArg: 1.071 ± 0.025	1.17MetSer: 1.17 ± 0.027	1.314MetThr: 1.314 ± 0.028	1.215MetVal: 1.215 ± 0.028	0.185MetTrp: 0.185 ± 0.009	0.537MetTyr: 0.537 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.422AsnAla: 3.422 ± 0.045	0.282AsnCys: 0.282 ± 0.013	2.042AsnAsp: 2.042 ± 0.04	2.044AsnGlu: 2.044 ± 0.037	1.866AsnPhe: 1.866 ± 0.038	3.709AsnGly: 3.709 ± 0.054	0.746AsnHis: 0.746 ± 0.019	2.203AsnIle: 2.203 ± 0.047	1.732AsnLys: 1.732 ± 0.036	4.202AsnLeu: 4.202 ± 0.058	0.75AsnMet: 0.75 ± 0.021	1.881AsnAsn: 1.881 ± 0.048	2.716AsnPro: 2.716 ± 0.041	1.737AsnGln: 1.737 ± 0.032	2.661AsnArg: 2.661 ± 0.043	2.342AsnSer: 2.342 ± 0.044	2.658AsnThr: 2.658 ± 0.045	2.826AsnVal: 2.826 ± 0.044	0.693AsnTrp: 0.693 ± 0.02	1.873AsnTyr: 1.873 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	4.961ProAla: 4.961 ± 0.062	0.239ProCys: 0.239 ± 0.013	3.223ProAsp: 3.223 ± 0.042	3.742ProGlu: 3.742 ± 0.048	2.191ProPhe: 2.191 ± 0.035	3.955ProGly: 3.955 ± 0.048	0.786ProHis: 0.786 ± 0.025	2.039ProIle: 2.039 ± 0.034	2.07ProLys: 2.07 ± 0.037	4.128ProLeu: 4.128 ± 0.053	0.877ProMet: 0.877 ± 0.02	1.823ProAsn: 1.823 ± 0.036	1.489ProPro: 1.489 ± 0.038	1.454ProGln: 1.454 ± 0.029	1.821ProArg: 1.821 ± 0.036	2.7ProSer: 2.7 ± 0.037	2.262ProThr: 2.262 ± 0.039	4.283ProVal: 4.283 ± 0.048	0.528ProTrp: 0.528 ± 0.019	1.644ProTyr: 1.644 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.352GlnAla: 3.352 ± 0.048	0.177GlnCys: 0.177 ± 0.011	1.592GlnAsp: 1.592 ± 0.028	2.163GlnGlu: 2.163 ± 0.04	1.663GlnPhe: 1.663 ± 0.029	2.28GlnGly: 2.28 ± 0.037	0.749GlnHis: 0.749 ± 0.023	2.079GlnIle: 2.079 ± 0.035	2.134GlnLys: 2.134 ± 0.036	4.026GlnLeu: 4.026 ± 0.047	0.736GlnMet: 0.736 ± 0.019	1.669GlnAsn: 1.669 ± 0.038	1.874GlnPro: 1.874 ± 0.032	1.887GlnGln: 1.887 ± 0.04	2.011GlnArg: 2.011 ± 0.035	2.221GlnSer: 2.221 ± 0.035	2.421GlnThr: 2.421 ± 0.034	2.865GlnVal: 2.865 ± 0.044	0.508GlnTrp: 0.508 ± 0.015	1.499GlnTyr: 1.499 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	3.686ArgAla: 3.686 ± 0.048	0.331ArgCys: 0.331 ± 0.015	2.684ArgAsp: 2.684 ± 0.037	3.686ArgGlu: 3.686 ± 0.055	3.139ArgPhe: 3.139 ± 0.041	3.148ArgGly: 3.148 ± 0.048	1.148ArgHis: 1.148 ± 0.026	3.742ArgIle: 3.742 ± 0.048	3.64ArgLys: 3.64 ± 0.047	6.163ArgLeu: 6.163 ± 0.073	1.414ArgMet: 1.414 ± 0.027	2.755ArgAsn: 2.755 ± 0.043	2.512ArgPro: 2.512 ± 0.037	2.754ArgGln: 2.754 ± 0.044	3.344ArgArg: 3.344 ± 0.049	3.21ArgSer: 3.21 ± 0.041	3.135ArgThr: 3.135 ± 0.039	3.638ArgVal: 3.638 ± 0.048	0.873ArgTrp: 0.873 ± 0.024	2.579ArgTyr: 2.579 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	5.18SerAla: 5.18 ± 0.051	0.518SerCys: 0.518 ± 0.019	3.226SerAsp: 3.226 ± 0.047	3.033SerGlu: 3.033 ± 0.045	3.414SerPhe: 3.414 ± 0.043	5.718SerGly: 5.718 ± 0.068	1.119SerHis: 1.119 ± 0.023	3.302SerIle: 3.302 ± 0.049	2.525SerLys: 2.525 ± 0.037	6.738SerLeu: 6.738 ± 0.067	1.151SerMet: 1.151 ± 0.024	2.329SerAsn: 2.329 ± 0.049	2.909SerPro: 2.909 ± 0.047	2.085SerGln: 2.085 ± 0.035	3.5SerArg: 3.5 ± 0.048	3.773SerSer: 3.773 ± 0.053	3.414SerThr: 3.414 ± 0.048	4.758SerVal: 4.758 ± 0.059	0.929SerTrp: 0.929 ± 0.027	2.606SerTyr: 2.606 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.26ThrAla: 5.26 ± 0.056	0.394ThrCys: 0.394 ± 0.015	3.526ThrAsp: 3.526 ± 0.047	3.143ThrGlu: 3.143 ± 0.047	2.935ThrPhe: 2.935 ± 0.047	5.555ThrGly: 5.555 ± 0.068	1.04ThrHis: 1.04 ± 0.024	3.24ThrIle: 3.24 ± 0.044	2.339ThrLys: 2.339 ± 0.036	6.218ThrLeu: 6.218 ± 0.063	0.904ThrMet: 0.904 ± 0.023	2.342ThrAsn: 2.342 ± 0.045	3.219ThrPro: 3.219 ± 0.039	1.96ThrGln: 1.96 ± 0.034	3.085ThrArg: 3.085 ± 0.04	3.508ThrSer: 3.508 ± 0.048	3.612ThrThr: 3.612 ± 0.067	4.494ThrVal: 4.494 ± 0.067	0.84ThrTrp: 0.84 ± 0.025	2.422ThrTyr: 2.422 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	5.705ValAla: 5.705 ± 0.056	0.572ValCys: 0.572 ± 0.018	3.409ValAsp: 3.409 ± 0.046	3.637ValGlu: 3.637 ± 0.044	3.522ValPhe: 3.522 ± 0.05	4.541ValGly: 4.541 ± 0.063	1.236ValHis: 1.236 ± 0.024	3.895ValIle: 3.895 ± 0.054	3.395ValLys: 3.395 ± 0.051	6.971ValLeu: 6.971 ± 0.068	1.348ValMet: 1.348 ± 0.027	2.991ValAsn: 2.991 ± 0.046	3.343ValPro: 3.343 ± 0.051	2.294ValGln: 2.294 ± 0.032	4.401ValArg: 4.401 ± 0.052	5.231ValSer: 5.231 ± 0.057	4.59ValThr: 4.59 ± 0.057	5.253ValVal: 5.253 ± 0.056	0.95ValTrp: 0.95 ± 0.023	2.506ValTyr: 2.506 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	1.018TrpAla: 1.018 ± 0.025	0.107TrpCys: 0.107 ± 0.008	0.721TrpAsp: 0.721 ± 0.02	0.858TrpGlu: 0.858 ± 0.023	0.671TrpPhe: 0.671 ± 0.019	1.056TrpGly: 1.056 ± 0.024	0.311TrpHis: 0.311 ± 0.013	0.802TrpIle: 0.802 ± 0.025	0.987TrpLys: 0.987 ± 0.024	1.589TrpLeu: 1.589 ± 0.034	0.403TrpMet: 0.403 ± 0.016	0.702TrpAsn: 0.702 ± 0.02	0.473TrpPro: 0.473 ± 0.019	0.677TrpGln: 0.677 ± 0.017	0.686TrpArg: 0.686 ± 0.017	0.84TrpSer: 0.84 ± 0.02	0.854TrpThr: 0.854 ± 0.021	0.941TrpVal: 0.941 ± 0.023	0.264TrpTrp: 0.264 ± 0.012	0.541TrpTyr: 0.541 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.065TyrAla: 3.065 ± 0.043	0.29TyrCys: 0.29 ± 0.013	2.225TyrAsp: 2.225 ± 0.036	2.045TyrGlu: 2.045 ± 0.032	2.075TyrPhe: 2.075 ± 0.033	2.905TyrGly: 2.905 ± 0.048	0.807TyrHis: 0.807 ± 0.019	1.592TyrIle: 1.592 ± 0.03	1.629TyrLys: 1.629 ± 0.035	4.154TyrLeu: 4.154 ± 0.048	0.563TyrMet: 0.563 ± 0.019	1.704TyrAsn: 1.704 ± 0.04	1.856TyrPro: 1.856 ± 0.025	1.733TyrGln: 1.733 ± 0.034	2.572TyrArg: 2.572 ± 0.039	2.455TyrSer: 2.455 ± 0.044	2.29TyrThr: 2.29 ± 0.042	2.392TyrVal: 2.392 ± 0.035	0.563TyrTrp: 0.563 ± 0.017	1.653TyrTyr: 1.653 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4964 proteins (1846786 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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