Beihai narna-like virus 11

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KI60|A0A1L3KI60_9VIRU Uncharacterized protein OS=Beihai narna-like virus 11 OX=1922438 PE=4 SV=1
MM1 pKa = 7.52ASDD4 pKa = 3.41HH5 pKa = 6.22RR6 pKa = 11.84TDD8 pKa = 3.1KK9 pKa = 11.09GDD11 pKa = 3.34RR12 pKa = 11.84VEE14 pKa = 4.34AKK16 pKa = 9.65TYY18 pKa = 10.84LSLIDD23 pKa = 4.93VISQSLRR30 pKa = 11.84PSGDD34 pKa = 2.98GKK36 pKa = 10.91ASSCCRR42 pKa = 11.84CYY44 pKa = 10.25EE45 pKa = 4.12ASSLACQLIRR55 pKa = 11.84RR56 pKa = 11.84HH57 pKa = 5.9SGVLGSAVVKK67 pKa = 10.42KK68 pKa = 10.23RR69 pKa = 11.84SKK71 pKa = 10.48GKK73 pKa = 10.16EE74 pKa = 3.79VIQWPDD80 pKa = 3.62FPQGDD85 pKa = 4.39VVSSLYY91 pKa = 8.8FTRR94 pKa = 11.84SYY96 pKa = 11.52LEE98 pKa = 3.75VLIDD102 pKa = 4.25FDD104 pKa = 6.09DD105 pKa = 6.38RR106 pKa = 11.84LLKK109 pKa = 10.26WNWKK113 pKa = 7.67HH114 pKa = 6.22LSVIFEE120 pKa = 4.49EE121 pKa = 4.69CMSWEE126 pKa = 3.71PLNFIKK132 pKa = 10.45HH133 pKa = 6.11AKK135 pKa = 9.34FCTAVPMADD144 pKa = 3.36YY145 pKa = 11.3LNRR148 pKa = 11.84KK149 pKa = 8.38LKK151 pKa = 10.36NPRR154 pKa = 11.84PALVEE159 pKa = 3.69RR160 pKa = 11.84VGPYY164 pKa = 9.98FRR166 pKa = 11.84GPLKK170 pKa = 10.45RR171 pKa = 11.84YY172 pKa = 6.32LTNRR176 pKa = 11.84INTGRR181 pKa = 11.84GKK183 pKa = 10.04NLSLAWSILQGVKK196 pKa = 10.24RR197 pKa = 11.84GCAPASDD204 pKa = 4.87EE205 pKa = 4.2YY206 pKa = 11.38LQSEE210 pKa = 4.74YY211 pKa = 11.28NSYY214 pKa = 11.68ADD216 pKa = 5.23LLGEE220 pKa = 4.47PPSNPYY226 pKa = 10.48SDD228 pKa = 3.4YY229 pKa = 9.8TRR231 pKa = 11.84YY232 pKa = 10.3YY233 pKa = 10.37SEE235 pKa = 3.57IWDD238 pKa = 3.63GLRR241 pKa = 11.84VRR243 pKa = 11.84RR244 pKa = 11.84EE245 pKa = 3.61THH247 pKa = 5.42EE248 pKa = 4.51FYY250 pKa = 10.31PSKK253 pKa = 10.35SAKK256 pKa = 10.3LGFNRR261 pKa = 11.84SEE263 pKa = 4.17GGGVQGMVRR272 pKa = 11.84SMDD275 pKa = 3.69LEE277 pKa = 5.73AGLVDD282 pKa = 5.42MVDD285 pKa = 3.23TDD287 pKa = 3.11KK288 pKa = 11.67GVIEE292 pKa = 4.02RR293 pKa = 11.84RR294 pKa = 11.84GVNIPPLSDD303 pKa = 3.5LLTAEE308 pKa = 4.39SEE310 pKa = 4.47EE311 pKa = 4.17PCIVRR316 pKa = 11.84VQGLQEE322 pKa = 3.8PLKK325 pKa = 10.87VRR327 pKa = 11.84FITKK331 pKa = 9.43SPSVRR336 pKa = 11.84SYY338 pKa = 10.21VCKK341 pKa = 10.24PIQNALWNHH350 pKa = 5.85LKK352 pKa = 10.42EE353 pKa = 4.45SEE355 pKa = 4.05PFALIGHH362 pKa = 6.82PIRR365 pKa = 11.84RR366 pKa = 11.84EE367 pKa = 3.73DD368 pKa = 4.22LRR370 pKa = 11.84QLKK373 pKa = 10.0IKK375 pKa = 9.49SLRR378 pKa = 11.84FDD380 pKa = 4.71FGPQPFWVSGDD391 pKa = 3.67YY392 pKa = 10.74KK393 pKa = 10.92SATDD397 pKa = 5.5RR398 pKa = 11.84IDD400 pKa = 3.73LRR402 pKa = 11.84QTKK405 pKa = 10.11DD406 pKa = 3.24ALEE409 pKa = 4.34CALAEE414 pKa = 4.26LLLHH418 pKa = 5.63GHH420 pKa = 6.41IGLRR424 pKa = 11.84QADD427 pKa = 3.39ACRR430 pKa = 11.84RR431 pKa = 11.84EE432 pKa = 4.84LYY434 pKa = 9.91EE435 pKa = 3.82QTITFPLHH443 pKa = 5.85LQMPSVPQGNGQLMGSVLSFPILCVINLAAYY474 pKa = 6.84WSSMEE479 pKa = 4.58EE480 pKa = 4.2YY481 pKa = 10.11FQEE484 pKa = 4.56EE485 pKa = 4.76YY486 pKa = 10.69RR487 pKa = 11.84LQDD490 pKa = 3.64LPVLINGDD498 pKa = 3.96DD499 pKa = 3.9IGFQSNDD506 pKa = 2.45EE507 pKa = 5.05HH508 pKa = 7.37YY509 pKa = 10.86AIWQQKK515 pKa = 6.23IHH517 pKa = 6.14EE518 pKa = 4.65VGLKK522 pKa = 10.45LSIGKK527 pKa = 9.89NYY529 pKa = 9.69IHH531 pKa = 6.78PTYY534 pKa = 9.78FTINSEE540 pKa = 4.09LWNMCDD546 pKa = 3.0LTQVHH551 pKa = 6.67FFNLGCLTNMNRR563 pKa = 11.84RR564 pKa = 11.84TGRR567 pKa = 11.84EE568 pKa = 4.1CTKK571 pKa = 10.45SLPIAEE577 pKa = 4.81LWSEE581 pKa = 4.21VHH583 pKa = 6.84VGATNKK589 pKa = 9.82DD590 pKa = 3.56RR591 pKa = 11.84AWRR594 pKa = 11.84RR595 pKa = 11.84FVHH598 pKa = 5.69YY599 pKa = 9.62NKK601 pKa = 10.04PQIQSATLRR610 pKa = 11.84GRR612 pKa = 11.84LNLGLPYY619 pKa = 9.97TAGGLNCSIPCDD631 pKa = 3.67YY632 pKa = 10.68RR633 pKa = 11.84VTDD636 pKa = 3.78GQRR639 pKa = 11.84RR640 pKa = 11.84LQRR643 pKa = 11.84YY644 pKa = 8.03CEE646 pKa = 3.47RR647 pKa = 11.84LYY649 pKa = 11.03RR650 pKa = 11.84NEE652 pKa = 3.31GRR654 pKa = 11.84TLTGVKK660 pKa = 9.92GRR662 pKa = 11.84SHH664 pKa = 6.71MLHH667 pKa = 5.32KK668 pKa = 10.27HH669 pKa = 4.54GRR671 pKa = 11.84SNNMRR676 pKa = 11.84LYY678 pKa = 10.5DD679 pKa = 3.51GTYY682 pKa = 10.29EE683 pKa = 4.35VILSSSEE690 pKa = 3.82GQEE693 pKa = 3.34QRR695 pKa = 11.84VPVEE699 pKa = 4.23LLDD702 pKa = 4.35APRR705 pKa = 11.84EE706 pKa = 4.12DD707 pKa = 3.72IILPVKK713 pKa = 10.48IPTEE717 pKa = 3.97THH719 pKa = 6.72LSPFGPILADD729 pKa = 3.82DD730 pKa = 3.77EE731 pKa = 4.91VEE733 pKa = 4.5NKK735 pKa = 9.91FSYY738 pKa = 10.27PSRR741 pKa = 11.84KK742 pKa = 10.31DD743 pKa = 2.89FDD745 pKa = 3.75QAKK748 pKa = 9.44KK749 pKa = 9.53FAPGGSPKK757 pKa = 10.38NFCLTKK763 pKa = 10.26KK764 pKa = 10.35VPYY767 pKa = 9.87DD768 pKa = 3.3KK769 pKa = 10.99RR770 pKa = 11.84FVTGKK775 pKa = 9.74VIKK778 pKa = 8.6VTPYY782 pKa = 10.67

Molecular weight: 89.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KI60|A0A1L3KI60_9VIRU Uncharacterized protein OS=Beihai narna-like virus 11 OX=1922438 PE=4 SV=1
MM1 pKa = 7.81SNITKK6 pKa = 10.45AKK8 pKa = 9.27FFQRR12 pKa = 11.84PSLKK16 pKa = 10.45GLPKK20 pKa = 9.87QEE22 pKa = 4.26KK23 pKa = 7.44EE24 pKa = 4.29RR25 pKa = 11.84RR26 pKa = 11.84WKK28 pKa = 8.91QHH30 pKa = 4.93LMSLGGEE37 pKa = 4.19TPRR40 pKa = 11.84NSRR43 pKa = 11.84RR44 pKa = 11.84PSRR47 pKa = 11.84RR48 pKa = 11.84NKK50 pKa = 9.07VAVGRR55 pKa = 11.84MKK57 pKa = 10.62TMPEE61 pKa = 3.9DD62 pKa = 3.7EE63 pKa = 4.58RR64 pKa = 11.84CGLEE68 pKa = 4.07YY69 pKa = 9.76ATSLINPFDD78 pKa = 4.11FSLNPCVPRR87 pKa = 11.84MPSVPSRR94 pKa = 11.84KK95 pKa = 9.24LCTYY99 pKa = 11.04ASGLGSTSGTTSYY112 pKa = 11.48GGVTAYY118 pKa = 7.47MTAANDD124 pKa = 3.86TTSVAVTEE132 pKa = 4.38TAFTGLSLPAWNSTGASSKK151 pKa = 8.0TQNSPYY157 pKa = 10.52SGADD161 pKa = 3.6FSANGAQVRR170 pKa = 11.84LVSAGLKK177 pKa = 10.05VRR179 pKa = 11.84FTGTKK184 pKa = 9.07LNQGGVCFPFLEE196 pKa = 5.01PDD198 pKa = 3.43LGDD201 pKa = 3.47VTGFTANDD209 pKa = 2.84IAAYY213 pKa = 9.6DD214 pKa = 3.49QYY216 pKa = 11.5FQGIEE221 pKa = 4.02FDD223 pKa = 4.17SDD225 pKa = 3.18WVSITFTPRR234 pKa = 11.84HH235 pKa = 5.73PADD238 pKa = 3.73FDD240 pKa = 3.55FAAADD245 pKa = 3.63KK246 pKa = 10.18PLTGQHH252 pKa = 6.37PCMGILIMSAAPNQPFEE269 pKa = 4.65YY270 pKa = 9.97VWTAHH275 pKa = 5.95WEE277 pKa = 4.44VIGRR281 pKa = 11.84NARR284 pKa = 11.84GKK286 pKa = 7.74TLSHH290 pKa = 7.23TYY292 pKa = 10.29PNTDD296 pKa = 3.77RR297 pKa = 11.84IISGASQFPPRR308 pKa = 11.84ALSAIQNAPRR318 pKa = 11.84PEE320 pKa = 3.93SLAAQVGRR328 pKa = 11.84NLMAHH333 pKa = 6.49GAGVVEE339 pKa = 4.18SLGRR343 pKa = 11.84GAMNMVGAAVADD355 pKa = 3.99TMVSGAAGLLSIGVV369 pKa = 3.65

Molecular weight: 39.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1151

369

782

575.5

64.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.69 ± 2.125

2.085 ± 0.374

5.039 ± 0.499

5.3 ± 0.909

3.91 ± 0.357

7.732 ± 0.897

2.259 ± 0.324

4.431 ± 0.603

5.213 ± 0.726

9.209 ± 1.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.172 ± 0.553

4.257 ± 0.179

6.082 ± 0.355

3.823 ± 0.153

7.124 ± 0.595

7.993 ± 0.486

5.387 ± 1.266

5.995 ± 0.017

1.564 ± 0.107

3.736 ± 0.664
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski