Lactococcus phage 98202
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8BM51|A0A1P8BM51_9CAUD Resistance protein OS=Lactococcus phage 98202 OX=1868858 GN=DS98202_18 PE=4 SV=1
MM1 pKa = 7.55 KK2 pKa = 9.96 CDD4 pKa = 3.13 KK5 pKa = 10.52 CGNEE9 pKa = 4.13 IDD11 pKa = 4.27 CDD13 pKa = 4.25 CMGCHH18 pKa = 6.48 YY19 pKa = 7.79 FTQQLHH25 pKa = 5.69 PVSLGAYY32 pKa = 8.74 EE33 pKa = 4.65 SEE35 pKa = 4.45 EE36 pKa = 4.04 LTKK39 pKa = 10.76 DD40 pKa = 3.4 KK41 pKa = 11.0 QIEE44 pKa = 4.1 LL45 pKa = 3.9
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.499
IPC2_protein 4.685
IPC_protein 4.482
Toseland 4.342
ProMoST 4.609
Dawson 4.444
Bjellqvist 4.584
Wikipedia 4.329
Rodwell 4.329
Grimsley 4.253
Solomon 4.431
Lehninger 4.38
Nozaki 4.558
DTASelect 4.698
Thurlkill 4.368
EMBOSS 4.342
Sillero 4.609
Patrickios 0.54
IPC_peptide 4.431
IPC2_peptide 4.596
IPC2.peptide.svr19 4.521
Protein with the highest isoelectric point:
>tr|A0A1P8BM23|A0A1P8BM23_9CAUD Integrase OS=Lactococcus phage 98202 OX=1868858 GN=DS98202_01 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.35 FGMRR6 pKa = 11.84 KK7 pKa = 9.38 PSITKK12 pKa = 9.06 ILKK15 pKa = 10.36 AITTTKK21 pKa = 10.48 YY22 pKa = 9.62 KK23 pKa = 10.61 RR24 pKa = 11.84 KK25 pKa = 9.38 VKK27 pKa = 10.28 KK28 pKa = 10.44 ALIPGYY34 pKa = 9.54 GKK36 pKa = 10.74 KK37 pKa = 10.71 GMGWIKK43 pKa = 10.5 NPKK46 pKa = 7.92 KK47 pKa = 9.89 AAYY50 pKa = 9.48 NKK52 pKa = 9.59 VYY54 pKa = 10.79 KK55 pKa = 9.53 KK56 pKa = 7.59 TTFSIWGLFNN66 pKa = 4.27
Molecular weight: 7.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.153
IPC2_protein 10.555
IPC_protein 10.584
Toseland 11.096
ProMoST 10.584
Dawson 11.184
Bjellqvist 10.76
Wikipedia 11.286
Rodwell 11.959
Grimsley 11.199
Solomon 11.213
Lehninger 11.199
Nozaki 11.052
DTASelect 10.76
Thurlkill 11.067
EMBOSS 11.462
Sillero 11.082
Patrickios 11.681
IPC_peptide 11.213
IPC2_peptide 9.268
IPC2.peptide.svr19 7.801
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
11063
45
918
208.7
23.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.237 ± 0.306
0.597 ± 0.115
6.11 ± 0.187
7.041 ± 0.653
4.601 ± 0.185
6.309 ± 0.549
1.428 ± 0.182
7.105 ± 0.376
9.383 ± 0.654
7.873 ± 0.332
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.45 ± 0.164
6.183 ± 0.317
2.748 ± 0.205
4.239 ± 0.301
3.679 ± 0.292
6.463 ± 0.382
6.291 ± 0.276
5.785 ± 0.291
1.338 ± 0.122
4.14 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here