Xanthomonas phage Carpasina
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1GSQ5|A0A2S1GSQ5_9CAUD DNA ligase OS=Xanthomonas phage Carpasina OX=2163636 PE=4 SV=1
MM1 pKa = 7.24 YY2 pKa = 10.15 KK3 pKa = 10.09 VEE5 pKa = 4.81 LNPVPNQTVSFNLDD19 pKa = 2.97 GAFWQIHH26 pKa = 5.46 IFQSISFMCADD37 pKa = 3.22 ISLNGRR43 pKa = 11.84 RR44 pKa = 11.84 VISGVRR50 pKa = 11.84 CFGGFGLMPYY60 pKa = 10.44 SYY62 pKa = 8.0 MTAPNFGNFVFDD74 pKa = 6.08 FDD76 pKa = 5.77 ADD78 pKa = 3.41 WTGFGSTCSLYY89 pKa = 10.87 FLDD92 pKa = 4.32 AVEE95 pKa = 4.73 FKK97 pKa = 10.68 EE98 pKa = 4.38 FQSLMLSGEE107 pKa = 4.08
Molecular weight: 12.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.145
IPC2_protein 4.444
IPC_protein 4.279
Toseland 4.088
ProMoST 4.329
Dawson 4.253
Bjellqvist 4.507
Wikipedia 4.19
Rodwell 4.113
Grimsley 4.012
Solomon 4.24
Lehninger 4.202
Nozaki 4.38
DTASelect 4.584
Thurlkill 4.139
EMBOSS 4.19
Sillero 4.393
Patrickios 0.846
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.364
Protein with the highest isoelectric point:
>tr|A0A2S1GSU6|A0A2S1GSU6_9CAUD Uncharacterized protein OS=Xanthomonas phage Carpasina OX=2163636 PE=4 SV=1
MM1 pKa = 7.57 DD2 pKa = 3.4 QVAASGLIVAAASPSVKK19 pKa = 10.01 LYY21 pKa = 9.94 QKK23 pKa = 9.06 MRR25 pKa = 11.84 RR26 pKa = 11.84 GEE28 pKa = 3.89 LRR30 pKa = 11.84 TPLKK34 pKa = 10.18 AQSNRR39 pKa = 11.84 RR40 pKa = 11.84 QFAGIKK46 pKa = 10.11 AEE48 pKa = 4.21 SPKK51 pKa = 10.05 PQQAPALKK59 pKa = 9.03 PGLVSGEE66 pKa = 3.93
Molecular weight: 7.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.263
IPC2_protein 9.78
IPC_protein 10.306
Toseland 10.979
ProMoST 10.555
Dawson 11.023
Bjellqvist 10.672
Wikipedia 11.199
Rodwell 11.433
Grimsley 11.052
Solomon 11.155
Lehninger 11.125
Nozaki 10.935
DTASelect 10.672
Thurlkill 10.95
EMBOSS 11.359
Sillero 10.95
Patrickios 11.199
IPC_peptide 11.155
IPC2_peptide 9.209
IPC2.peptide.svr19 8.724
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
86
0
86
19008
55
942
221.0
24.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.238 ± 0.394
0.921 ± 0.085
5.898 ± 0.212
6.497 ± 0.325
3.967 ± 0.197
7.534 ± 0.374
1.563 ± 0.133
6.034 ± 0.265
5.871 ± 0.309
7.423 ± 0.193
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.699 ± 0.135
4.461 ± 0.221
4.24 ± 0.179
3.967 ± 0.144
5.324 ± 0.201
7.071 ± 0.253
5.492 ± 0.254
6.887 ± 0.197
1.268 ± 0.106
2.646 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here