Actinoplanes brasiliensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae;

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8372 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6JC80|A0A4R6JC80_9ACTN Putative phosphosugar-binding protein OS=Actinoplanes brasiliensis OX=52695 GN=C8E87_7581 PE=4 SV=1
MM1 pKa = 7.75SDD3 pKa = 2.77HH4 pKa = 7.29HH5 pKa = 7.4DD6 pKa = 4.23FGDD9 pKa = 3.9HH10 pKa = 7.33DD11 pKa = 5.64DD12 pKa = 4.34YY13 pKa = 11.52PIFDD17 pKa = 4.05TPEE20 pKa = 3.61AHH22 pKa = 6.96DD23 pKa = 3.44THH25 pKa = 8.4DD26 pKa = 5.2DD27 pKa = 3.83FGALDD32 pKa = 4.75PIAYY36 pKa = 9.01DD37 pKa = 3.5DD38 pKa = 3.95HH39 pKa = 8.49HH40 pKa = 7.38FDD42 pKa = 3.43TPEE45 pKa = 3.9FDD47 pKa = 3.85VPEE50 pKa = 4.1VHH52 pKa = 7.04HH53 pKa = 7.32DD54 pKa = 3.34ISADD58 pKa = 3.32EE59 pKa = 4.25TPPVDD64 pKa = 3.47EE65 pKa = 4.7HH66 pKa = 6.47TGLAADD72 pKa = 4.24EE73 pKa = 4.31VAAAEE78 pKa = 4.33PLDD81 pKa = 4.14VFPPALDD88 pKa = 3.63VGDD91 pKa = 4.32LPEE94 pKa = 4.88PVDD97 pKa = 3.75GFPWIDD103 pKa = 3.28TGSLGVVHH111 pKa = 7.02AAAAGHH117 pKa = 6.46AVDD120 pKa = 4.02PVRR123 pKa = 11.84PEE125 pKa = 3.72EE126 pKa = 3.82LAEE129 pKa = 3.99YY130 pKa = 10.49AGVEE134 pKa = 4.06LSPAQDD140 pKa = 2.87PWAALAADD148 pKa = 4.59EE149 pKa = 5.54DD150 pKa = 4.66PATSALARR158 pKa = 11.84WWQEE162 pKa = 3.47NN163 pKa = 3.32

Molecular weight: 17.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6JWW9|A0A4R6JWW9_9ACTN Putative transcriptional regulator OS=Actinoplanes brasiliensis OX=52695 GN=C8E87_3404 PE=3 SV=1
MM1 pKa = 7.61KK2 pKa = 10.21VRR4 pKa = 11.84NSLRR8 pKa = 11.84SLKK11 pKa = 10.24AKK13 pKa = 9.56PGSVVTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84QVVINRR30 pKa = 11.84KK31 pKa = 7.11NPRR34 pKa = 11.84WNGRR38 pKa = 11.84QGG40 pKa = 3.01

Molecular weight: 4.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8372

0

8372

2891309

29

18732

345.4

36.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.773 ± 0.044

0.676 ± 0.007

5.962 ± 0.022

5.235 ± 0.024

2.797 ± 0.014

9.268 ± 0.032

2.1 ± 0.012

3.532 ± 0.021

1.925 ± 0.022

10.407 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.734 ± 0.011

2.0 ± 0.018

6.164 ± 0.029

2.804 ± 0.015

8.039 ± 0.035

5.174 ± 0.024

6.119 ± 0.024

8.724 ± 0.027

1.556 ± 0.011

2.011 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski