Agrobacterium phage Milano

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Schmittlotzvirus; unclassified Schmittlotzvirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MG03|A0A482MG03_9CAUD Uncharacterized protein OS=Agrobacterium phage Milano OX=2557550 GN=Milano_090 PE=4 SV=1
MM1 pKa = 7.21ITPVCPSVPCGNPPVPLPPPPSPPPSTGGAIVVEE35 pKa = 4.43ASDD38 pKa = 4.26TIILTGDD45 pKa = 3.66GTADD49 pKa = 3.27NPLRR53 pKa = 11.84AEE55 pKa = 3.89LAFPLRR61 pKa = 11.84IDD63 pKa = 4.18LASTSYY69 pKa = 11.38DD70 pKa = 3.24GLSDD74 pKa = 4.05NDD76 pKa = 3.43FQMVYY81 pKa = 10.49SAVEE85 pKa = 3.73SFNFVNAEE93 pKa = 3.9STGAVSPILSTVLNVTVNDD112 pKa = 3.83ADD114 pKa = 3.87AGNITVDD121 pKa = 3.0NGVFSFALSEE131 pKa = 4.55SILVNPGDD139 pKa = 3.61VVKK142 pKa = 10.51IINSGPIVFDD152 pKa = 3.38YY153 pKa = 11.11LAITIVGNVTPP164 pKa = 4.88

Molecular weight: 16.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MHE7|A0A482MHE7_9CAUD Virion-associated protein OS=Agrobacterium phage Milano OX=2557550 GN=Milano_021 PE=4 SV=1
MM1 pKa = 7.38RR2 pKa = 11.84QSKK5 pKa = 6.43TARR8 pKa = 11.84PGEE11 pKa = 4.11MVRR14 pKa = 11.84AVVKK18 pKa = 10.61LKK20 pKa = 10.98GGAKK24 pKa = 8.7SARR27 pKa = 11.84VFIYY31 pKa = 10.34SRR33 pKa = 11.84YY34 pKa = 9.84NGIGDD39 pKa = 4.3FSPHH43 pKa = 6.53VDD45 pKa = 3.46DD46 pKa = 5.2AAIGVLEE53 pKa = 4.13NGKK56 pKa = 8.88PVLARR61 pKa = 11.84PFSIRR66 pKa = 11.84LDD68 pKa = 3.63RR69 pKa = 11.84EE70 pKa = 3.96LKK72 pKa = 7.98TRR74 pKa = 11.84NVFSIMASEE83 pKa = 4.59AIEE86 pKa = 3.99VQVIEE91 pKa = 4.57EE92 pKa = 4.5LDD94 pKa = 3.54FGQEE98 pKa = 3.28INTRR102 pKa = 11.84ARR104 pKa = 11.84IVIHH108 pKa = 6.45GKK110 pKa = 9.93ASQAFGRR117 pKa = 11.84IFRR120 pKa = 11.84NIRR123 pKa = 11.84RR124 pKa = 11.84FFRR127 pKa = 11.84RR128 pKa = 11.84KK129 pKa = 7.8TPSAA133 pKa = 3.57

Molecular weight: 15.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

127

0

127

21525

31

778

169.5

18.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.929 ± 0.272

1.412 ± 0.127

5.965 ± 0.178

6.425 ± 0.271

4.437 ± 0.156

7.726 ± 0.252

1.761 ± 0.128

5.58 ± 0.16

5.213 ± 0.237

6.987 ± 0.166

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.546 ± 0.136

4.451 ± 0.184

4.999 ± 0.227

3.261 ± 0.148

6.272 ± 0.264

5.552 ± 0.172

6.276 ± 0.25

7.517 ± 0.261

1.449 ± 0.088

3.243 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski