Sesame yellow mosaic virus
Average proteome isoelectric point is 7.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4FSB4|A0A2Z4FSB4_9GEMI Replication enhancer OS=Sesame yellow mosaic virus OX=2231646 PE=3 SV=1
MM1 pKa = 7.76 AYY3 pKa = 9.26 SAPRR7 pKa = 11.84 IVVPIRR13 pKa = 11.84 AFSYY17 pKa = 9.79 KK18 pKa = 9.7 KK19 pKa = 10.07 RR20 pKa = 11.84 RR21 pKa = 11.84 FYY23 pKa = 11.1 SLKK26 pKa = 9.9 EE27 pKa = 3.66 FLFAPLEE34 pKa = 4.19 ALLKK38 pKa = 11.09 SGDD41 pKa = 4.01 FPVGDD46 pKa = 3.7 YY47 pKa = 11.34 RR48 pKa = 11.84 EE49 pKa = 4.2 FDD51 pKa = 3.84 LDD53 pKa = 3.8 NLIEE57 pKa = 4.68 FYY59 pKa = 10.69 RR60 pKa = 11.84 SEE62 pKa = 3.82 NRR64 pKa = 11.84 SIKK67 pKa = 10.3 EE68 pKa = 3.93 LFEE71 pKa = 5.0 DD72 pKa = 4.41 EE73 pKa = 4.48 YY74 pKa = 11.05 VVRR77 pKa = 11.84 RR78 pKa = 11.84 EE79 pKa = 3.87 EE80 pKa = 4.35 GKK82 pKa = 9.87 DD83 pKa = 3.1 AVYY86 pKa = 10.05 PRR88 pKa = 11.84 VRR90 pKa = 11.84 PEE92 pKa = 3.69 EE93 pKa = 4.13 PLPVQEE99 pKa = 4.52 VRR101 pKa = 11.84 RR102 pKa = 11.84 KK103 pKa = 9.24 EE104 pKa = 3.87 IPISDD109 pKa = 4.39 SEE111 pKa = 4.8 CPCCSKK117 pKa = 10.92 EE118 pKa = 4.1 KK119 pKa = 10.87 GVDD122 pKa = 3.56 EE123 pKa = 4.49 VV124 pKa = 3.8
Molecular weight: 14.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.152
IPC2_protein 5.321
IPC_protein 5.156
Toseland 5.003
ProMoST 5.219
Dawson 5.067
Bjellqvist 5.219
Wikipedia 4.889
Rodwell 4.991
Grimsley 4.914
Solomon 5.067
Lehninger 5.016
Nozaki 5.169
DTASelect 5.27
Thurlkill 4.991
EMBOSS 4.914
Sillero 5.245
Patrickios 4.202
IPC_peptide 5.08
IPC2_peptide 5.245
IPC2.peptide.svr19 5.339
Protein with the highest isoelectric point:
>tr|A0A2Z4FRS8|A0A2Z4FRS8_9GEMI C2 OS=Sesame yellow mosaic virus OX=2231646 PE=3 SV=1
MM1 pKa = 7.66 KK2 pKa = 9.68 MGNLISTCLSNSKK15 pKa = 10.14 ARR17 pKa = 11.84 SSSITPVISTYY28 pKa = 10.93 GILVPAEE35 pKa = 3.82 TSIQISRR42 pKa = 11.84 EE43 pKa = 4.0 LNPAPMSSPISRR55 pKa = 11.84 RR56 pKa = 11.84 TEE58 pKa = 3.51 ISSTGVNFRR67 pKa = 11.84 SMEE70 pKa = 4.25 DD71 pKa = 3.19 LQEE74 pKa = 4.11 EE75 pKa = 4.68 VNRR78 pKa = 11.84 RR79 pKa = 11.84 LMMHH83 pKa = 5.87 VRR85 pKa = 11.84 RR86 pKa = 11.84 HH87 pKa = 4.44
Molecular weight: 9.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.297
IPC_protein 10.058
Toseland 10.394
ProMoST 10.101
Dawson 10.511
Bjellqvist 10.233
Wikipedia 10.716
Rodwell 10.599
Grimsley 10.555
Solomon 10.657
Lehninger 10.628
Nozaki 10.409
DTASelect 10.218
Thurlkill 10.409
EMBOSS 10.804
Sillero 10.452
Patrickios 10.496
IPC_peptide 10.657
IPC2_peptide 9.385
IPC2.peptide.svr19 8.431
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1128
87
373
188.0
21.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.433 ± 0.499
1.507 ± 0.257
5.053 ± 0.485
5.94 ± 1.608
4.787 ± 0.54
5.762 ± 0.6
2.926 ± 0.778
6.383 ± 0.433
6.56 ± 0.593
7.181 ± 0.879
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.837 ± 1.044
5.319 ± 0.651
6.294 ± 0.62
3.901 ± 0.971
6.56 ± 1.225
8.156 ± 0.833
5.319 ± 1.064
5.496 ± 0.924
1.684 ± 0.42
3.901 ± 0.393
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here